t(3t(3!ID U89936; SV 1; circular; genomic DNA; STD; SYN; 5271 BP. AC U89936; DT 19-JUN-1997 (Rel. 52, Created) DT 04-MAR-2000 (Rel. 63, Last updated, Version 5) DE Cloning vector p6xHis-GFP, complete sequence. KW . OS unidentified cloning vector OC other sequences; artificial sequences; vectors. RN [1] RP 1-5271 RA Kitts P.; RT "p6xHis-GFP, complete sequence"; RL Unpublished. RN [2] RP 1-5271 RA Holtz A., Lou Y.; RT ; RL Submitted (18-FEB-1997) to the EMBL/GenBank/DDBJ databases. RL CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA RL 94303-4230, USA CC This vector can be obtained from CLONTECH Laboratories, Inc., 1020 CC East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an CC order, call (415) 424-8222 or (800) 662-2566, extension 1. CC International customers, please contact your local distributor or CC subsidiary. For technical information, call (415) 424-8222 or (800) CC 662-2566, extension 3. CC This sequence has been compiled from information in the sequence CC databases, published literature and other sources, together with CC partial sequences obtained by CLONTECH. If you suspect there is an CC error in this sequence, please contact CLONTECH's Technical Support CC Department at (415) 424-8222 or (800) 662-2566, extension 3 or CC E-mail TECH@CLONTECH.COM. FH Key Location/Qualifiers FT source 1..5271 FT /organism="unidentified cloning vector" FT /mol_type="genomic DNA" FT /db_xref="taxon:45196" SQ Sequence 5271 BP; 1382 A; 1249 C; 1324 G; 1316 T; 0 other; gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa 300 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta 360 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg 420 ttctcatcat catcatcatc atggtatggc tagcaaagga gaagaacttt tcactggagt 480 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg 540 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg 600 aaaactacct gttccatggc caacacttgt cactactttc tcttatggtg ttcaatgctt 660 ttcaagatac ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg 720 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga 780 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa 840 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata 900 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat 960 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg 1020 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc 1080 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca 1140 tggcatggat gaactataca aataaatgtc cagacttcca attgacacta aagtgtccga 1200 acaattacta aaatctcagg gttcctggtt aaattcaggc tgagatatta tttatatatt 1260 tatagattca ttaaaactgt atgaataatt tattgatgtt attgatagag gttattttct 1320 tattaaacag gctacttgga gtgtattctt aattctatat taattacaat ttgatttgac 1380 ttgctcaaaa agcggccgcg gcggccgcga attcgaagct tggctgtttt ggcggatgag 1440 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga 1500 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga 1560 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg 1620 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1680 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 1740 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag 1800 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt tgtttatttt 1860 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1920 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 1980 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 2040 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 2100 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2160 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 2220 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2280 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2340 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2400 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2460 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2520 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2580 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2640 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2700 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2760 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2820 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2880 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2940 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 3000 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 3060 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 3120 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3180 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3240 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3300 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3360 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3420 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3480 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3540 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3600 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3660 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3720 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 3780 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 3840 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 3900 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 3960 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 4020 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc 4080 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg 4140 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 4200 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 4260 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 4320 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 4380 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 4440 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 4500 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 4560 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 4620 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 4680 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 4740 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 4800 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 4860 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 4920 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 4980 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 5040 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 5100 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 5160 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 5220 agcgggcagt gagcgcaacg caattaatgt gagttagcgc gaattgatct g 5271  6xHis GFP"Times New Roman!t(3CM13_pUC_rev_pri"Times New Romant(3CMrrnB_ATS"Times New Romant(3CɆrpTrcHis_fwd_pri"Times New Romant(3minicis"Times New Romant(3OFR 2"Times New Romant(3C6xHis"Times New Romant(3C GFP(variant)"Times New Romant(3C GFP_R_primer"Times New Roman<t(3CɆ GFPfusionREV_pri"Times New Roman<t(3CNb GFP_F_pri"Times New Roman<t(3CɆ pBAD_rev_pri"Times New Romant(3pTrcHis_rev_pri"Times New Romant(3C}rrnB_terminator"Times New Romant(3C[ rrnB_T1_term"Times New Romant(3CɆ % rrnB_T2_term"Times New Romant(3OkAmpR_pro"Times New 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