Sequence of 6xHisGFP

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Sequence of 6xHisGFP

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Sequence of 6xHis GFP: 5271 BP; 1382 A; 1249 C; 1324 G; 1316 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtttgacagc ttatcatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc 60

1 caaactgtcg aatagtagct gacgtgccac gtggttacga agaccgcagt ccgtcggtag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggaagctgtg gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc 120

61 ccttcgacac cataccgaca cgtccagcat ttagtgacgt attaagcaca gcgagttccg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcactcccgt tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc 180

121 cgtgagggca agacctatta caaaaaacgc ggctgtagta ttgccaagac cgtttataag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgaaatgagc tgttgacaat taatcatccg gctcgtataa tgtgtggaat tgtgagcgga 240

181 actttactcg acaactgtta attagtaggc cgagcatatt acacacctta acactcgcct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 taacaatttc acacaggaaa cagcgccgct gagaaaaagc gaagcggcac tgctctttaa 300

241 attgttaaag tgtgtccttt gtcgcggcga ctctttttcg cttcgccgtg acgagaaatt 300

M13_pUC_rev_pri .... .......... .......... rrnB_ATS ..........

.......... .......... .......... .......... .......... ..........

301 caatttatca gacaatctgt gtgggcactc gaccggaatt atcgattaac tttattatta 360

301 gttaaatagt ctgttagaca cacccgtgag ctggccttaa tagctaattg aaataataat 360

.......... rrnB_ATS .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aaaattaaag aggtatatat taatgtatcg attaaataag gaggaataaa ccatgggggg 420

361 ttttaatttc tccatatata attacatagc taatttattc ctccttattt ggtacccccc 420

....... pTrcHis_fwd_pri ....... minicis .......... ..........

.......... .......... NheI(449) .......... .......... ..........

421 ttctcatcat catcatcatc atggtatggc tagcaaagga gaagaacttt tcactggagt 480

421 aagagtagta gtagtagtag taccataccg atcgtttcct cttcttgaaa agtgacctca 480

.......... 6xHis OFR 2 ......... GFP(variant) .........

.......... .......... .......... .......... .......... ..........

481 tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg 540

481 acagggttaa gaacaactta atctaccact acaattaccc gtgtttaaaa gacagtcacc 540

..... GFP_R_primer OFR 2 GFP(variant) ..... ....... GFPfusionREV_pri

.......... .......... .......... .......... .......... ..........

541 agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg 600

541 tctcccactt ccactacgtt gtatgccttt tgaatgggaa tttaaataaa cgtgatgacc 600

.......... ...... OFR 2 GFP(variant) ...... .......... ..........

.......... MscI(619) .......... .......... .......... ..........

601 aaaactacct gttccatggc caacacttgt cactactttc tcttatggtg ttcaatgctt 660

601 ttttgatgga caaggtaccg gttgtgaaca gtgatgaaag agaataccac aagttacgaa 660

.......... ...... OFR 2 GFP(variant) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttcaagatac ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg 720

661 aagttctatg ggtctagtat actttgccgt actgaaaaag ttctcacggt acgggcttcc 720

.......... ...... OFR 2 GFP(variant) ...... .......... ..........

.......... .......... .......... .......... .......... PmlI(773)

721 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga 780

721 aatacatgtc ctttcttgat ataaaaagtt tctactgccc ttgatgttct gtgcacgact 780

.......... ...... OFR 2 GFP(variant) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa 840

781 tcagttcaaa cttccactat gggaacaatt atcttagctc aattttccat aactaaaatt 840

.......... ...... OFR 2 GFP(variant) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata 900

841 tcttctacct ttgtaagaac ctgtgtttaa ccttatgttg atattgagtg tgttacatat 900

.......... ...... OFR 2 GFP(variant) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

901 catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat 960

901 gtagtaccgt ctgtttgttt tcttacctta gtttcaattg aagttttaat ctgtgttgta 960

.......... ...... OFR 2 GFP(variant) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg 1020

961 acttctacct tcgcaagttg atcgtctggt aatagttgtt ttatgaggtt aaccgctacc 1020

.......... ...... OFR 2 GFP(variant) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc 1080

1021 gggacaggaa aatggtctgt tggtaatgga caggtgtgtt agacgggaaa gctttctagg 1080

.......... ...... OFR 2 GFP(variant) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca 1140

1081 gttgcttttc tctctggtgt accaggaaga actcaaacat tgtcgacgac cctaatgtgt 1140

.......... ...... OFR 2 GFP(variant) GFP_F_pri ...... ..........

.......... .......... .......... .......... .......... ..........

1141 tggcatggat gaactataca aataaatgtc cagacttcca attgacacta aagtgtccga 1200

1141 accgtaccta cttgatatgt ttatttacag gtctgaaggt taactgtgat ttcacaggct 1200

...... OFR 2 GFP(variant) ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 acaattacta aaatctcagg gttcctggtt aaattcaggc tgagatatta tttatatatt 1260

1201 tgttaatgat tttagagtcc caaggaccaa tttaagtccg actctataat aaatatataa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tatagattca ttaaaactgt atgaataatt tattgatgtt attgatagag gttattttct 1320

1261 atatctaagt aattttgaca tacttattaa ataactacaa taactatctc caataaaaga 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tattaaacag gctacttgga gtgtattctt aattctatat taattacaat ttgatttgac 1380

1321 ataatttgtc cgatgaacct cacataagaa ttaagatata attaatgtta aactaaactg 1380

.......... .......... .......... .......... .......... ..........

...... SacII(1399) EcoRI(1409) HindIII(1416) ...... ..........

1381 ttgctcaaaa agcggccgcg gcggccgcga attcgaagct tggctgtttt ggcggatgag 1440

1381 aacgagtttt tcgccggcgc cgccggcgct taagcttcga accgacaaaa ccgcctactc 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga 1500

1441 tcttctaaaa gtcggactat gtctaattta gtcttgcgtc ttcgccagac tattttgtct 1500

.......... . pBAD_rev_pri pTrcHis_rev_pri . .......... ..........

.......... .......... .......... .......... .......... ..........

1501 atttgcctgg cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga 1560

1501 taaacggacc gccgtcatcg cgccaccagg gtggactggg gtacggcttg agtcttcact 1560

.......... .......... ....... rrnB_terminator ....... ..........

.......... .......... .......... .......... .......... ..........

1561 aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg aactgccagg 1620

1561 ttgcggcatc gcggctacca tcacacccca gaggggtacg ctctcatccc ttgacggtcc 1620

.......... ....... rrnB_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 1680

1621 gtagtttatt ttgctttccg agtcagcttt ctgacccgga aagcaaaata gacaacaaac 1680

.......... . rrnB_terminator rrnB_T1_term . .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag 1740

1681 agccacttgc gagaggactc atcctgttta ggcggccctc gcctaaactt gcaacgcttc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag 1800

1741 gttgccgggc ctcccaccgc ccgtcctgcg ggcggtattt gacggtccgt agtttaattc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctttt tgtttatttt 1860

1801 gtcttccggt aggactgcct accggaaaaa cgcaaagatg tttgagaaaa acaaataaaa 1860

......... rrnB_T2_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 1920

1861 agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt tacgaagtta 1920

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt a ttccctttt 1980

1921 ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa taagggaaaa 1980

.......... .......... ....... Ampicillin OFR 3 ....... ..........

.......... .......... .......... .......... .......... ..........

1981 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 2040

1981 aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt cattttctac 2040

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 2100

2041 gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg tcgccattct 2100

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 2160

2101 aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa tttcaagacg 2160

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac 2220

2161 atacaccgcg ccataatagg gcacaactgc ggcccgttct cgttgagcca gcggcgtatg 2220

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 2280

2221 tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta gaatgcctac 2280

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 2340

2281 cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg tgacgccggt 2340

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 2400

2341 tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac gtgttgtacc 2400

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 2460

2401 ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg tatggtttgc 2460

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... FspI(2505) ..........

2461 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 2520

2461 tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt gataattgac 2520

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 2580

2521 cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc cgcctatttc 2580

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 2640

2581 aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga ctatttagac 2640

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 2700

2641 ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta ccattcggga 2700

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 2760

2701 gggcatagca tcaatagatg tgctgcccct cagtccgttg atacctactt gctttatctg 2760

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 2820

2761 tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg gttcaaatga 2820

.......... ....... Ampicillin OFR 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 2880

2821 gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag atccacttct 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 2940

2881 aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag gtgactcgca 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 3000

2941 gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac gcgcattaga 3000

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3001 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 3060

3001 cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc ctagttctcg 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 3120

3061 atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt ttatgacagg 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 3180

3121 aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc ggatgtatgg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 3240

3181 agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc acagaatggc 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 3300

3241 ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact tgccccccaa 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 3360

3301 gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg gatgtcgcac 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 3420

3361 tcgatactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata ggccattcgc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 3480

3421 cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg accatagaaa 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 3540

3481 tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact acgagcagtc 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 3600

3541 cccccgcctc ggataccttt ttgcggtcgt tgcgccggaa aaatgccaag gaccggaaaa 3600

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 3660

3601 cgaccggaaa acgagtgtac aagaaaggac gcaatagggg actaagacac ctattggcat 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 3720

3661 aatggcggaa actcactcga ctatggcgag cggcgtcggc ttgctggctc gcgtcgctca 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 3780

3721 gtcactcgct ccttcgcctt ctcgcggact acgccataaa agaggaatgc gtagacacgc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa 3840

3781 cataaagtgt ggcgtatacc acgtgagagt catgttagac gagactacgg cgtatcaatt 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc 3900

3841 cggtcatatg tgaggcgata gcgatgcact gacccagtac cgacgcgggg ctgtgggcgg 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 3960

3901 ttgtgggcga ctgcgcggga ctgcccgaac agacgagggc cgtaggcgaa tgtctgttcg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 4020

3961 acactggcag aggccctcga cgtacacagt ctccaaaagt ggcagtagtg gctttgcgcg 4020

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcatttacgt tgacaccatc 4080

4021 ctccgtcgtc tagttaagcg cgcgcttccg cttcgccgta cgtaaatgca actgtggtag 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag tcaattcagg 4140

4081 cttaccgcgt tttggaaagc gccataccgt actatcgcgg gccttctctc agttaagtcc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 4200

4141 caccacttac actttggtca ttgcaatatg ctacagcgtc tcatacggcc acagagaata 4200

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 4260

4201 gtctggcaaa gggcgcacca cttggtccgg tcggtgcaaa gacgcttttg cgcccttttt 4260

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 4320

4261 caccttcgcc gctaccgcct cgacttaatg taagggttgg cgcaccgtgt tgttgaccgc 4320

.......... ......... lacI Orf frame 1 ......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 4380

4321 ccgtttgtca gcaacgacta accgcaacgg tggaggtcag accgggacgt gcgcggcagc 4380

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 4440

4381 gtttaacagc gccgctaatt tagagcgcgg ctagttgacc cacggtcgca ccaccacagc 4440

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 4500

4441 taccatcttg cttcgccgca gcttcggaca tttcgccgcc acgtgttaga agagcgcgtt 4500

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... BclI(4514) .......... .......... .......... ..........

4501 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 4560

4501 gcgcagtcac ccgactagta attgataggc gacctactgg tcctacggta acgacacctt 4560

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 4620

4561 cgacggacgt gattacaagg ccgcaataaa gaactacaga gactggtctg tgggtagttg 4620

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 4680

4621 tcataataaa agagggtact tctgccatgc gctgacccgc acctcgtaga ccagcgtaac 4680

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... ApaI(4711) .......... ..........

4681 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 4740

4681 ccagtggtcg tttagcgcga caatcgcccg ggtaattcaa gacagagccg cgcagacgca 4740

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 4800

4741 gaccgaccga ccgtatttat agagtgagcg ttagtttaag tcggctatcg ccttgccctt 4800

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 4860

4801 ccgctgacct cacggtacag gccaaaagtt gtttggtacg tttacgactt actcccgtag 4860

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 4920

4861 caagggtgac gctacgacca acggttgcta gtctaccgcg acccgcgtta cgcgcggtaa 4920

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... ......... EcoRV(4950) ......... .......... ..........

4921 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 4980

4921 tggctcaggc ccgacgcgca accacgccta tagagccatc accctatgct gctatggctt 4980

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 5040

4981 ctgtcgagta caatataggg cggcaattgg tggtagtttg tcctaaaagc ggacgacccc 5040

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 5100

5041 gtttggtcgc acctggcgaa cgacgttgag agagtcccgg tccgccactt cccgttagtc 5100

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... NarI(5140) .......... ..........

5101 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 5160

5101 gacaacgggc agagtgacca cttttctttt tggtgggacc gcgggttatg cgtttggcgg 5160

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 5220

5161 agaggggcgc gcaaccggct aagtaattac gtcgaccgtg ctgtccaaag ggctgacctt 5220

.......... ....... lacI Orf frame 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 agcgggcagt gagcgcaacg caattaatgt gagttagcgc gaattgatct g......... 5271

5221 tcgcccgtca ctcgcgttgc gttaattaca ctcaatcgcg cttaactaga c......... 5271

lacI Orf frame 1 .... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-5271 Reference author: Kitts P.; Reference title: "p6xHis-GFP, complete sequence"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-5271 Reference author: Holtz A., Lou Y.; Reference title: ; Reference location: Submitted (18-FEB-1997) to the EMBL/GenBank/DDBJ databases. Reference location: CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA Reference location: 94303-4230, USA