r[>'r[>'a)ID FJ475055; SV 1; circular; other DNA; STD; SYN; 5746 BP. AC FJ475055; DT 29-DEC-2008 (Rel. 98, Created) DT 29-DEC-2008 (Rel. 98, Last updated, Version 1) DE Cloning vector AbVec-hIgG1, complete sequence. KW . OS Cloning vector AbVec-hIgG1 OC other sequences; artificial sequences; vectors. RN [1] RP 1-5746 RA Wilson P.C.; RT "Antibody variable gene expression vector for human IgG1 heavy chain"; RL Unpublished. RN [2] RP 1-5746 RA Wilson P.C.; RT ; RL Submitted (18-NOV-2008) to the EMBL/GenBank/DDBJ databases. RL Medicine/Rheumatology, The University of Chicago, BSLC/JFK Bldg. 924 East RL 57th St., Rm 414, Chicago, IL 60637, USA FH Key Location/Qualifiers FT source 1..5746 FT /organism="Cloning vector AbVec-hIgG1" FT /mol_type="other DNA" FT /note="expression cloning vector for human antibody FT variable genes" FT /db_xref="taxon:583342" FT promoter 1..618 FT /note="CMV" FT misc_feature 929..973 FT /note="murine IgG1 signal peptide; derived from FT DEC205/immunoglobluin gamma fusion protein in GenBank FT Accession Number DQ407610" FT misc_feature 974..991 FT /note="heavy chain variable gene cloning site; flanked by FT AgeI and SalI sites" FT gene <992..1984 FT /gene="IgG1" FT CDS <992..1984 FT /codon_start=1 FT /transl_table=11 FT /gene="IgG1" FT /product="IgG1" FT /note="Cgamma-1; derived from Homo sapiens" FT /protein_id="ACK87036.1" FT /translation="RSTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGA FT LTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCD FT KTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVD FT GVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKA FT KGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVL FT DSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK" FT gene 3709..4569 FT /gene="Amp_Res" FT CDS 3709..4569 FT /codon_start=1 FT /transl_table=11 FT /gene="Amp_Res" FT /product="beta-lactamase" FT /note="confers resistance to ampicillin" FT /protein_id="ACK87037.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" SQ Sequence 5746 BP; 1400 A; 1564 C; 1399 G; 1383 T; 0 other; ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca 60 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 120 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 180 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 240 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 300 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 360 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 420 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 480 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 540 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt 600 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga 660 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt 720 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta 780 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac 840 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc 900 acctcggttc tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt 960 agcaactgca accggtgtac actcgagcgt acggtcgacc aagggcccat cggtcttccc 1020 cctggcaccc tcctccaaga gcacctctgg gggcacagcg gccctgggct gcctggtcaa 1080 ggactacttc cccgaacctg tgacggtctc gtggaactca ggcgccctga ccagcggcgt 1140 gcacaccttc ccggctgtcc tacagtcctc aggactctac tccctcagca gcgtggtgac 1200 cgtgccctcc agcagcttgg gcacccagac ctacatctgc aacgtgaatc acaagcccag 1260 caacaccaag gtggacaaga aagttgagcc caaatcttgt gacaaaactc acacatgccc 1320 accgtgccca gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc 1380 caaggacacc ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag 1440 ccacgaagac cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc 1500 caagacaaag ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac 1560 cgtcctgcac caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc 1620 cctcccagcc cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca 1680 ggtgtacacc ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg 1740 cctggtcaaa ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc 1800 ggagaacaac tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta 1860 cagcaagctc accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt 1920 gatgcatgag gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa 1980 atgaagcttg gccgccatgg cccaacttgt ttattgcagc ttataatggt tacaaataaa 2040 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 2100 tgtccaaact catcaatgta tcttatcatg tctggatcga tcgggaatta attcggcgca 2160 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga 2220 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 2280 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 2340 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 2400 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 2460 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 2520 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctgttaaca 2580 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 2640 ttaatcgcct tgcagcacat ccccccttcg ccagctggcg taatagcgaa gaggcccgca 2700 ccgatcgccc ttcccaacag ttgcgtagcc tgaatggcga atggcgcctg atgcggtatt 2760 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc 2820 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 2880 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 2940 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 3000 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc 3060 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 3120 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg 3180 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 3240 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc 3300 tgatgccgca tagttaagcc aactccgcta tcgctacgtg actgggtcat ggctgcgccc 3360 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 3420 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 3480 ccgaaacgcg cgaggcagta ttcttgaaga cgaaagggcc tcgtgatacg cctattttta 3540 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 3600 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 3660 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 3720 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 3780 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 3840 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 3900 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgatgacgcc 3960 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 4020 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 4080 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 4140 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 4200 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc agcagcaatg 4260 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 4320 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 4380 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 4440 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 4500 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 4560 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 4620 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 4680 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 4740 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 4800 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 4860 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 4920 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 4980 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 5040 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 5100 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg 5160 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 5220 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 5280 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 5340 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 5400 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 5460 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 5520 cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccagctggca cgacaggttt 5580 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttacct cactcattag 5640 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 5700 taacaatttc acacaggaaa cagctatgac catgattacg aattaa 5746 r AbVec-hIgG1"Times New Roman! r[>'$SacI(9),SpeI(29),NdeI(264),NcoI(390) Arialr\[\QiFiF r[>'2SacI(598),EagI(685),ClaI(914),EcoRI(920),NcoI(928) Arialr!))|a r[>'+XhoI(983),SalI(995),ApaI(1008),Bsu36I(1169) Arialr#Hmm r[>';Bsu36I(1411),Bsu36I(1453),XmaI(1702),SmaI(1704),SexAI(1723) Arialr5>GGg r[>'\HindIII(1985),NcoI(1996),SfiI(1998),ClaI(2138),NcoI(2165),KpnI(2208),PvuII(2230),SexAI(2321) ArialZrMJ r[>',NcoI(2461),SfiI(2507),HpaI(2577),PvuII(2675) Arial r&&g r[>'ScaI Arialr~uk8k r[>'PvuII Arialr(`&V#L@>Lr[>'r$8CMV_fwd_primer"Times New Romanr[>'rPcpCEP_fwd_primer"Times New Romanr[>'rRj LNCX_primer"Times New Romanr[>'Cr<M Sp6_primer"Times New Romanr[>'CɆrORF 2"Times New Romanr[>'CrIgG1"Times New Romanr[>'Cr/BEBV_rev_primer"Times New Romanr[>'rpBABE_3_primer"Times New Romanr[>'r  SV40_promoter"Times New Romanr[>'CɆrs  SV40_origin"Times New Romanr[>'Cr SV40pro_F_primer"Times New Romanr[>'Cr , M13_forward20_primer"Times New Romanr[>'CɆr% ; M13_pUC_fwd_primer"Times New Romanr[>'Cr D  f1_origin"Times New Romanr[>'Crs  pGEX_3_primer"Times New Romanr[>'CɆr7S AmpR_promoter"Times New Romanr[>'Cr}Amp_Res"Times New Romanr[>'CɆr}ORF 1"Times New Roman(r[>'CɆrt pBR322_origin"Times New Romanr[>'CɆr1 lac_promoter"Times New Romanr[>'Cr?UM13_pUC_rev_primer"Times New Romanr[>'CrTfM13_reverse_primer"Times New Roman r[>'test"Times New RomanGr~uKxu# r[>'test"Times New Roman: 21rs' r[>'test"Times New Roman=rG[}++)