Sequence of AbVec-hIgG1

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Sequence of AbVec-hIgG1

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Sequence of AbVec-hIgG1: 5746 BP; 1400 A; 1564 C; 1399 G; 1383 T; 0 other;

SacI(9) .......... .......... .......... .......... ..........

1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca 60

1 aagctcgagc gggctgtaac taataactga tcaataatta tcattagtta atgccccagt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 120

61 aatcaagtat cgggtatata cctcaaggcg caatgtattg aatgccattt accgggcgga 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 180

121 ccgactggcg ggttgctggg ggcgggtaac tgcagttatt actgcataca agggtatcat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 240

181 tgcggttatc cctgaaaggt aactgcagtt acccacctca taaatgccat ttgacgggtg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 300

241 aaccgtcatg tagttcacat agtatacggt tcatgcgggg gataactgca gttactgcca 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 360

301 tttaccgggc ggaccgtaat acgggtcatg tactggaata ccctgaaagg atgaaccgtc 360

.......... .......... .......... .......... .......... ..........

.......... .......... NcoI(390) .......... .......... ..........

361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 420

361 atgtagatgc ataatcagta gcgataatgg taccactacg ccaaaaccgt catgtagtta 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 480

421 cccgcaccta tcgccaaact gagtgcccct aaaggttcag aggtggggta actgcagtta 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 540

481 ccctcaaaca aaaccgtggt tttagttgcc ctgaaaggtt ttacagcatt gttgaggcgg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt 600

541 ggtaactgcg tttacccgcc atccgcacat gccaccctcc agatatattc gtctcgagca 600

........ CMV_fwd_primer ........ .......... ....... pCEP_fwd_primer

.......... .......... .......... .......... .......... ..........

601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga 660

601 aatcacttgg cagtctagcg gacctctgcg gtaggtgcga caaaactgga ggtatcttct 660

pCEP_fwd_primer LNCX_primer .. .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt 720

661 gtggccctgg ctaggtcgga ggcgccggcc cttgccacgt aaccttgcgc ctaaggggca 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta 780

721 cggttctcac tgcattcatg gcggatatct cagatatccg ggtgggggaa ccgaagcaat 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac 840

781 cttgcgccga tgttaattat gtattggaat acatagtatg tgtatgctaa atccactgtg 840

.......... .......... .......... .......... .......... Sp6_primer

.......... .......... .......... .......... .......... ..........

841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc 900

841 atatcttatt gtaggtgaaa cggaaagaga ggtgtccaca ggtgagggtc caggttgacg 900

.......... .......... .......... .......... .......... ..........

..... ClaI(914) EcoRI(920) NcoI(928) ..... .......... ..........

901 acctcggttc tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt 960

901 tggagccaag atagctaact taaggtggta ccctaccagt acatagtagg aaaaagatca 960

.......... .......... .......... .......... ORF 2 ..........

.......... .......... XhoI(983) SalI(995) .......... ..........

961 agcaactgca accggtgtac actcgagcgt acggtcgacc aagggcccat cg gtcttccc 1020

961 tcgttgacgt tggccacatg tgagctcgca tgccagctgg ttcccgggta gccagaaggg 1020

.......... .......... ORF 2 .......... IgG1 ..........

.......... .......... .......... .......... .......... ..........

1021 cctggcaccc tcctccaaga gcacctctgg gggcacagcg gccctgggct gcctggtcaa 1080

1021 ggaccgtggg aggaggttct cgtggagacc cccgtgtcgc cgggacccga cggaccagtt 1080

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggactacttc cccgaacctg tgacggtctc gtggaactca ggcgccctga ccagcggcgt 1140

1081 cctgatgaag gggcttggac actgccagag caccttgagt ccgcgggact ggtcgccgca 1140

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... ......... Bsu36I(1169) ......... .......... ..........

1141 gcacaccttc ccggctgtcc tacagtcctc aggactctac tccctcagca gcgtggtgac 1200

1141 cgtgtggaag ggccgacagg atgtcaggag tcctgagatg agggagtcgt cgcaccactg 1200

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cgtgccctcc agcagcttgg gcacccagac ctacatctgc aacgtgaatc acaagcccag 1260

1201 gcacgggagg tcgtcgaacc cgtgggtctg gatgtagacg ttgcacttag tgttcgggtc 1260

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 caacaccaag gtggacaaga aagttgagcc caaatcttgt gacaaaactc acacatgccc 1320

1261 gttgtggttc cacctgttct ttcaactcgg gtttagaaca ctgttttgag tgtgtacggg 1320

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 accgtgccca gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc 1380

1321 tggcacgggt cgtggacttg aggacccccc tggcagtcag aaggagaagg ggggttttgg 1380

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 caaggacacc ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag 1440

1381 gttcctgtgg gagtactaga gggcctgggg actccagtgt acgcaccacc acctgcactc 1440

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccacgaagac cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc 1500

1441 ggtgcttctg ggactccagt tcaagttgac catgcacctg ccgcacctcc acgtattacg 1500

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 caagacaaag ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac 1560

1501 gttctgtttc ggcgccctcc tcgtcatgtt gtcgtgcatg gcacaccagt cgcaggagtg 1560

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgtcctgcac caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc 1620

1561 gcaggacgtg gtcctgaccg acttaccgtt cctcatgttc acgttccaga ggttgtttcg 1620

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cctcccagcc cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca 1680

1621 ggagggtcgg gggtagctct tttggtagag gtttcggttt cccgtcgggg ctcttggtgt 1680

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .... XmaI(1702),SmaI(1704) ... SexAI(1723) .........

1681 ggtgtacacc ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg 1740

1681 ccacatgtgg gacgggggta gggccctact cgactggttc ttggtccagt cggactggac 1740

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cctggtcaaa ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc 1800

1741 ggaccagttt ccgaagatag ggtcgctgta gcggcacctc accctctcgt tacccgtcgg 1800

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggagaacaac tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta 1860

1801 cctcttgttg atgttctggt gcggagggca cgacctgagg ctgccgagga agaaggagat 1860

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cagcaagctc accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt 1920

1861 gtcgttcgag tggcacctgt tctcgtccac cgtcgtcccc ttgcagaaga gtacgaggca 1920

.......... .......... ORF 2 IgG1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gatgcatgag gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa 1980

1921 ctacgtactc cgagacgtgt tggtgatgtg cgtcttctcg gagagggaca gaggcccatt 1980

.......... .......... ORF 2 IgG1 .......... .......... ..........

HindIII(1985) ...... .......... .......... .......... ..........

1981 atgaagcttg gccgccatgg cccaacttgt ttattgcagc ttataatggt tacaaataaa 2040

1981 tacttcgaac cggcggtacc gggttgaaca aataacgtcg aatattacca atgtttattt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 2100

2041 cgttatcgta gtgtttaaag tgtttatttc gtaaaaaaag tgacgtaaga tcaacaccaa 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tgtccaaact catcaatgta tcttatcatg tctggatcga tcgggaatta attcggcgca 2160

2101 acaggtttga gtagttacat agaatagtac agacctagct agcccttaat taagccgcgt 2160

EBV_rev_primer ...... .......... .......... .......... ..........

NcoI(2165) .......... .......... .......... .......... ..........

2161 gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggcgga 2220

2161 cgtggtaccg gactttattg gagactttct ccttgaacca atccatggaa gactccgcct 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca 2280

2221 ttcttggtcg acaccttaca cacagtcaat cccacacctt tcaggggtcc gaggggtcgt 2280

.......... ........ pBABE_3_primer ...... SV40_promoter ........

.......... .......... .......... ......... SexAI(2321) .........

2281 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca 2340

2281 ccgtcttcat acgtttcgta cgtagagtta atcagtcgtt ggtccacacc tttcaggggt 2340

.......... ........ SV40_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 2400

2341 ccgaggggtc gtccgtcttc atacgtttcg tacgtagagt taatcagtcg ttggtatcag 2400

.......... ........ SV40_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 2460

2401 ggcggggatt gaggcgggta gggcggggat tgaggcgggt caaggcgggt aagaggcggg 2460

.......... ....... SV40_promoter SV40_origin ....... ..........

NcoI(2461) .......... .......... .......... .......... ..........

2461 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 2520

2461 gtaccgactg attaaaaaaa ataaatacgt ctccggctcc ggcggagccg gagactcgat 2520

SV40_origin SV40_promoter SV40pro_F_primer ........ ..........

.......... .......... .......... .......... .......... ..........

2521 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctgttaaca 2580

2521 aaggtcttca tcactcctcc gaaaaaacct ccggatccga aaacgttttt cgacaattgt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac 2640

2581 cgaaccgtga ccggcagcaa aatgttgcag cactgaccct tttgggaccg caatgggttg 2640

. M13_forward20_primer M13_pUC_fwd_primer . .......... ..........

.......... .......... ......... PvuII(2675) ......... ..........

2641 ttaatcgcct tgcagcacat ccccccttcg ccagctggcg taatagcgaa gaggcccgca 2700

2641 aattagcgga acgtcgtgta ggggggaagc ggtcgaccgc attatcgctt ctccgggcgt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccgatcgccc ttcccaacag ttgcgtagcc tgaatggcga atggcgcctg atgcggtatt 2760

2701 ggctagcggg aagggttgtc aacgcatcgg acttaccgct taccgcggac tacgccataa 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc 2820

2761 aagaggaatg cgtagacacg ccataaagtg tggcgtatgc agtttcgttg gtatcatgcg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 2880

2821 cgggacatcg ccgcgtaatt cgcgccgccc acaccaccaa tgcgcgtcgc actggcgatg 2880

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

2881 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 2940

2881 tgaacggtcg cgggatcgcg ggcgaggaaa gcgaaagaag ggaaggaaag agcggtgcaa 2940

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 3000

2941 gcggccgaaa ggggcagttc gagatttagc ccccgaggga aatcccaagg ctaaatcacg 3000

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc 3060

3001 aaatgccgtg gagctggggt tttttgaact aaacccacta ccaagtgcat cacccggtag 3060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 3120

3061 cgggactatc tgccaaaaag cgggaaactg caacctcagg tgcaagaaat tatcacctga 3120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg atttataagg 3180

3121 gaacaaggtt tgaccttgtt gtgagttggg atagagcccg ataagaaaac taaatattcc 3180

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 3240

3181 ctaaaacggc taaagccgga taaccaattt tttactcgac taaattgttt ttaaattgcg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc 3300

3241 cttaaaattg ttttataatt gcaaatgtta aaataccacg tgagagtcat gttagacgag 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgatgccgca tagttaagcc aactccgcta tcgctacgtg actgggtcat ggctgcgccc 3360

3301 actacggcgt atcaattcgg ttgaggcgat agcgatgcac tgacccagta ccgacgcggg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct 3420

3361 gctgtgggcg gttgtgggcg actgcgcggg actgcccgaa cagacgaggg ccgtaggcga 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca 3480

3421 atgtctgttc gacactggca gaggccctcg acgtacacag tctccaaaag tggcagtagt 3480

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

3481 ccgaaacgcg cgaggcagta ttcttgaaga cgaaagggcc tcgtgatacg cctattttta 3540

3481 ggctttgcgc gctccgtcat aagaacttct gctttcccgg agcactatgc ggataaaaat 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 3600

3541 atccaattac agtactatta ttaccaaaga atctgcagtc caccgtgaaa agccccttta 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 3660

3601 cacgcgcctt ggggataaac aaataaaaag atttatgtaa gtttatacat aggcgagtac 3660

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

3661 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 3720

3661 tctgttattg ggactattta cgaagttatt ataacttttt ccttctcata ctcataagtt 3720

.......... .......... .......... .......... ........ Amp_Res ORF 1

.......... .......... .......... .......... .......... ..........

3721 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 3780

3721 gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa cggaaggaca aaaacgagtg 3780

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 3840

3781 ggtctttgcg accactttca ttttctacga cttctagtca acccacgtgc tcacccaatg 3840

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 3900

3841 tagcttgacc tagagttgtc gccattctag gaactctcaa aagcggggct tcttgcaaaa 3900

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgatgacgcc 3960

3901 ggttactact cgtgaaaatt tcaagacgat acaccgcgcc ataatagggc actactgcgg 3960

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ScaI(4016)

3961 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 4020

3961 cccgttctcg ttgagccagc ggcgtatgtg ataagagtct tactgaacca actcatgagt 4020

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 4080

4021 ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt ctcttaatac gtcacgacgg 4080

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 4140

4081 tattggtact cactattgtg acgccggttg aatgaagact gttgctagcc tcctggcttc 4140

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 4200

4141 ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt gagcggaact agcaaccctt 4200

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc agcagcaatg 4260

4201 ggcctcgact tacttcggta tggtttgctg ctcgcactgt ggtgctacgg tcgtcgttac 4260

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 4320

4261 cgttgttgca acgcgtttga taattgaccg cttgatgaat gagatcgaag ggccgttgtt 4320

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 4380

4321 aattatctga cctacctccg cctatttcaa cgtcctggtg aagacgcgag ccgggaaggc 4380

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 4440

4381 cgaccgacca aataacgact atttagacct cggccactcg cacccagagc gccatagtaa 4440

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 4500

4441 cgtcgtgacc ccggtctacc attcgggagg gcatagcatc aatagatgtg ctgcccctca 4500

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 4560

4501 gtccgttgat acctacttgc tttatctgtc tagcgactct atccacggag tgactaattc 4560

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 4620

4561 gtaaccattg acagtctggt tcaaatgagt atatatgaaa tctaactaaa ttttgaagta 4620

Amp_Res ORF 1 ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 4680

4621 aaaattaaat tttcctagat ccacttctag gaaaaactat tagagtactg gttttaggga 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 4740

4681 attgcactca aaagcaaggt gactcgcagt ctggggcatc ttttctagtt tcctagaaga 4740

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 4800

4741 actctaggaa aaaaagacgc gcattagacg acgaacgttt gtttttttgg tggcgatggt 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 4860

4801 cgccaccaaa caaacggcct agttctcgat ggttgagaaa aaggcttcca ttgaccgaag 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 4920

4861 tcgtctcgcg tctatggttt atgacaggaa gatcacatcg gcatcaatcc ggtggtgaag 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 4980

4921 ttcttgagac atcgtggcgg atgtatggag cgagacgatt aggacaatgg tcaccgacga 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 5040

4981 cggtcaccgc tattcagcac agaatggccc aacctgagtt ctgctatcaa tggcctattc 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 5100

5041 cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg ggtcgaacct cgcttgctgg 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg 5160

5101 atgtggcttg actctatgga tgtcgcactc gtaactcttt cgcggtgcga agggcttccc 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 5220

5161 tctttccgcc tgtccatagg ccattcgccg tcccagcctt gtcctctcgc gtgctccctc 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 5280

5221 gaaggtcccc ctttgcggac catagaaata tcaggacagc ccaaagcggt ggagactgaa 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 5340

5281 ctcgcagcta aaaacactac gagcagtccc cccgcctcgg ataccttttt gcggtcgttg 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 5400

5341 cgccggaaaa atgccaagga ccggaaaacg accggaaaac gagtgtacaa gaaaggacgc 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 5460

5401 aataggggac taagacacct attggcataa tggcggaaac tcactcgact atggcgagcg 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata 5520

5461 gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct cgcgggttat 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... PvuII(5565) .........

5521 cgcaaaccgc ctctccccgc gcgttggccg attcattaat ccagctggca cgacaggttt 5580

5521 gcgtttggcg gagaggggcg cgcaaccggc taagtaatta ggtcgaccgt gctgtccaaa 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttacct cactcattag 5640

5581 gggctgacct ttcgcccgtc actcgcgttg cgttaattac actcaatgga gtgagtaatc 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 5700

5641 cgtggggtcc gaaatgtgaa atacgaaggc cgagcataca acacacctta acactcgcct 5700

.......... ......... lac_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 taacaatttc acacaggaaa cagctatgac catgattacg aattaa.... .......... 5746

5701 attgttaaag tgtgtccttt gtcgatactg gtactaatgc ttaatt.... .......... 5746

M13_pUC_rev_primer M13_reverse_primer .... .......... ..........

References

Reference number: [1] Reference positions: 1-5746 Reference author: Wilson P.C.; Reference title: "Antibody variable gene expression vector for human IgG1 heavy chain"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-5746 Reference author: Wilson P.C.; Reference title: ; Reference location: Submitted (18-NOV-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Medicine/Rheumatology, The University of Chicago, BSLC/JFK Bldg. 924 East Reference location: 57th St., Rm 414, Chicago, IL 60637, USA