W[#'W[#'ÿ+$ID FJ475056; SV 1; circular; other DNA; STD; SYN; 5074 BP. AC FJ475056; DT 29-DEC-2008 (Rel. 98, Created) DT 29-DEC-2008 (Rel. 98, Last updated, Version 1) DE Cloning vector AbVec-hIgKappa, complete sequence. KW . OS Cloning vector AbVec-hIgKappa OC other sequences; artificial sequences; vectors. RN [1] RP 1-5074 RA Wilson P.C.; RT "Ig-kappa expression vector for expression-cloning of antibody variable RT region genes"; RL Unpublished. RN [2] RP 1-5074 RA Wilson P.C., Wilson P.C., Wilson P.C.; RT ; RL Submitted (18-NOV-2008) to the EMBL/GenBank/DDBJ databases. RL Medicine/Rheumatology, The University of Chicago, BSLC/JFK Bldg. 924 East RL 57th St., Rm 414, Chicago, IL 60637, USA FH Key Location/Qualifiers FT source 1..5074 FT /organism="Cloning vector AbVec-hIgKappa" FT /mol_type="other DNA" FT /db_xref="taxon:583343" FT promoter 1..618 FT /note="CMV" FT misc_feature 929..973 FT /note="murine IgG1 signal peptide; derived from FT DEC205/immunoglobluin gamma fusion protein in GenBank FT Accession Number DQ407610" FT gene <989..1311 FT /gene="IgKappa" FT CDS <989..1311 FT /codon_start=3 FT /transl_table=11 FT /gene="IgKappa" FT /product="Igkappa constant region" FT /note="derived from Homo sapiens" FT /protein_id="ACK87038.1" FT /translation="TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNA FT LQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG FT EC" FT gene 3037..3897 FT /gene="Amp_Res" FT CDS 3037..3897 FT /codon_start=1 FT /transl_table=11 FT /gene="Amp_Res" FT /product="beta-lactamase" FT /note="confers resistance to ampicillin" FT /protein_id="ACK87039.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRDDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" SQ Sequence 5074 BP; 1254 A; 1317 C; 1223 G; 1280 T; 0 other; ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca 60 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 120 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 180 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 240 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 300 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 360 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 420 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 480 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 540 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt 600 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga 660 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt 720 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta 780 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac 840 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc 900 acctcggttc tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt 960 agcaactgca accggtgtac actcgagcgt acggtggctg caccatctgt cttcatcttc 1020 ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac 1080 ttctatccca gagaggccaa agtacagtgg aaggtggata acgccctcca atcgggtaac 1140 tcccaggaga gtgtcacaga gcaggacagc aaggacagca cctacagcct cagcagcacc 1200 ctgacgctga gcaaagcaga ctacgagaaa cacaaagtct acgcctgcga agtcacccat 1260 cagggcctga gctcgcccgt cacaaagagc ttcaacaggg gagagtgtta gaagcttggc 1320 cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 1380 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 1440 tcaatgtatc ttatcatgtc tggatcgatc gggaattaat tcggcgcagc accatggcct 1500 gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg 1560 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 1620 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 1680 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 1740 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 1800 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 1860 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc ttggcactgg 1920 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 1980 cagcacatcc ccccttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 2040 cccaacagtt gcgtagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc 2100 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg 2160 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 2220 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 2280 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 2340 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac 2400 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 2460 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat 2520 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 2580 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata 2640 gttaagccaa ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca 2700 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 2760 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 2820 aggcagtatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 2880 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 2940 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 3000 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 3060 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 3120 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 3180 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 3240 acttttaaag ttctgctatg tggcgcggta ttatcccgtg atgacgccgg gcaagagcaa 3300 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 3360 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 3420 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 3480 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 3540 gaagccatac caaacgacga gcgtgacacc acgatgccag cagcaatggc aacaacgttg 3600 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 3660 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 3720 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 3780 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 3840 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 3900 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 3960 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 4020 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 4080 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 4140 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 4200 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 4260 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 4320 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 4380 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4440 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac 4500 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4560 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4620 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 4680 cggttcctgg ccttttgctg gccttttgct 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