Sequence of AbVec-hIgKappa

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Sequence of AbVec-hIgKappa

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Sequence of AbVec-hIgKappa: 5074 BP; 1254 A; 1317 C; 1223 G; 1280 T; 0 other;

SacI(9) .......... .......... .......... .......... ..........

1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca 60

1 aagctcgagc gggctgtaac taataactga tcaataatta tcattagtta atgccccagt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 120

61 aatcaagtat cgggtatata cctcaaggcg caatgtattg aatgccattt accgggcgga 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 180

121 ccgactggcg ggttgctggg ggcgggtaac tgcagttatt actgcataca agggtatcat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 240

181 tgcggttatc cctgaaaggt aactgcagtt acccacctca taaatgccat ttgacgggtg 240

.......... .......... .......... .......... .......... ..........

.......... .......... NdeI(264) .......... .......... ..........

241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 300

241 aaccgtcatg tagttcacat agtatacggt tcatgcgggg gataactgca gttactgcca 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 360

301 tttaccgggc ggaccgtaat acgggtcatg tactggaata ccctgaaagg atgaaccgtc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 420

361 atgtagatgc ataatcagta gcgataatgg taccactacg ccaaaaccgt catgtagtta 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 480

421 cccgcaccta tcgccaaact gagtgcccct aaaggttcag aggtggggta actgcagtta 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 540

481 ccctcaaaca aaaccgtggt tttagttgcc ctgaaaggtt ttacagcatt gttgaggcgg 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt 600

541 ggtaactgcg tttacccgcc atccgcacat gccaccctcc agatatattc gtctcgagca 600

........ CMV_fwd_primer ........ .......... ....... pCEP_fwd_primer

.......... .......... .......... .......... .......... ..........

601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga 660

601 aatcacttgg cagtctagcg gacctctgcg gtaggtgcga caaaactgga ggtatcttct 660

pCEP_fwd_primer LNCX_primer .. .......... .......... ..........

.......... .......... EagI(685) .......... .......... ..........

661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt 720

661 gtggccctgg ctaggtcgga ggcgccggcc cttgccacgt aaccttgcgc ctaaggggca 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta 780

721 cggttctcac tgcattcatg gcggatatct cagatatccg ggtgggggaa ccgaagcaat 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac 840

781 cttgcgccga tgttaattat gtattggaat acatagtatg tgtatgctaa atccactgtg 840

.......... .......... .......... .......... .......... Sp6_primer

.......... .......... .......... .......... .......... ..........

841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc 900

841 atatcttatt gtaggtgaaa cggaaagaga ggtgtccaca ggtgagggtc caggttgacg 900

.......... .......... .......... .......... .......... ..........

..... ClaI(914) EcoRI(920) ..... .......... .......... ..........

901 acctcggttc tatcgattga attccaccat gggatggtca tgtatcatcc tttttctagt 960

901 tggagccaag atagctaact taaggtggta ccctaccagt acatagtagg aaaaagatca 960

.......... .......... .......... .......... .......... ..........

.......... .......... XhoI(983) .......... .......... ..........

961 agcaactgca accggtgtac actcgagcgt acggtggctg caccatctgt cttcatcttc 1020

961 tcgttgacgt tggccacatg tgagctcgca tgccaccgac gtggtagaca gaagtagaag 1020

.......... .......... .......... .......... IgKappa ..........

.......... .......... .......... .......... .......... ..........

1021 ccgccatctg atgagcagtt gaaatctgga actgcctctg ttgtgtgcct gctgaataac 1080

1021 ggcggtagac tactcgtcaa ctttagacct tgacggagac aacacacgga cgacttattg 1080

.......... .......... IgKappa .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttctatccca gagaggccaa agtacagtgg aaggtggata acgccctcca atcgggtaac 1140

1081 aagatagggt ctctccggtt tcatgtcacc ttccacctat tgcgggaggt tagcccattg 1140

.......... .......... IgKappa .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tcccaggaga gtgtcacaga gcaggacagc aaggacagca cctacagcct cagcagcacc 1200

1141 agggtcctct cacagtgtct cgtcctgtcg ttcctgtcgt ggatgtcgga gtcgtcgtgg 1200

.......... .......... IgKappa .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctgacgctga gcaaagcaga ctacgagaaa cacaaagtct acgcctgcga agtcacccat 1260

1201 gactgcgact cgtttcgtct gatgctcttt gtgtttcaga tgcggacgct tcagtgggta 1260

.......... .......... IgKappa .......... .......... ..........

.......... SacI(1274) .......... .......... .......... ..........

1261 cagggcctga gctcgcccgt cacaaagagc ttcaacaggg gagagtgtta gaagcttggc 1320

1261 gtcccggact cgagcgggca gtgtttctcg aagttgtccc ctctcacaat cttcgaaccg 1320

.......... .......... IgKappa .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cgccatggcc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 1380

1321 gcggtaccgg gttgaacaaa taacgtcgaa tattaccaat gtttatttcg ttatcgtagt 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 1440

1381 gtttaaagtg tttatttcgt aaaaaaagtg acgtaagatc aacaccaaac aggtttgagt 1440

.......... .......... .......... .......... ........ EBV_rev_primer

.......... .......... ClaI(1466) .......... .......... ..........

1441 tcaatgtatc ttatcatgtc tggatcgatc gggaattaat tcggcgcagc accatggcct 1500

1441 agttacatag aatagtacag acctagctag cccttaatta agccgcgtcg tggtaccgga 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... KpnI(1536) ......... PvuII(1558)

1501 gaaataacct ctgaaagagg aacttggtta ggtaccttct gaggcggaaa gaaccagctg 1560

1501 ctttattgga gactttctcc ttgaaccaat ccatggaaga ctccgccttt cttggtcgac 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg 1620

1561 accttacaca cagtcaatcc cacacctttc aggggtccga ggggtcgtcc gtcttcatac 1620

........ pBABE_3_primer ...... SV40_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

1621 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca 1680

1621 gtttcgtacg tagagttaat cagtcgttgg tccacacctt tcaggggtcc gaggggtcgt 1680

.......... ........ SV40_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact 1740

1681 ccgtcttcat acgtttcgta cgtagagtta atcagtcgtt ggtatcaggg cggggattga 1740

.......... ........ SV40_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 1800

1741 ggcgggtagg gcggggattg aggcgggtca aggcgggtaa gaggcggggt accgactgat 1800

.......... ....... SV40_promoter SV40_origin ....... ..........

.......... .......... .......... .......... .......... ..........

1801 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 1860

1801 taaaaaaaat aaatacgtct ccggctccgg cggagccgga gactcgataa ggtcttcatc 1860

SV40_origin SV40pro_F_primer SV40_promoter ........ ..........

.......... .......... .......... .......... HpaI(1905) ..........

1861 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tgttaacagc ttggcactgg 1920

1861 actcctccga aaaaacctcc ggatccgaaa acgtttttcg acaattgtcg aaccgtgacc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg 1980

1921 ggcagcaaaa tgttgcagca ctgacccttt tgggaccgca atgggttgaa ttagcggaac 1980

M13_forward20_primer M13_pUC_fwd_primer .. .......... ..........

.......... ......... PvuII(2003) ......... .......... ..........

1981 cagcacatcc ccccttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt 2040

1981 gtcgtgtagg ggggaagcgg tcgaccgcat tatcgcttct ccgggcgtgg ctagcgggaa 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cccaacagtt gcgtagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc 2100

2041 gggttgtcaa cgcatcggac ttaccgctta ccgcggacta cgccataaaa gaggaatgcg 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg 2160

2101 tagacacgcc ataaagtgtg gcgtatgcag tttcgttggt atcatgcgcg ggacatcgcc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc 2220

2161 gcgtaattcg cgccgcccac accaccaatg cgcgtcgcac tggcgatgtg aacggtcgcg 2220

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc 2280

2221 ggatcgcggg cgaggaaagc gaaagaaggg aaggaaagag cggtgcaagc ggccgaaagg 2280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct 2340

2281 ggcagttcga gatttagccc ccgagggaaa tcccaaggct aaatcacgaa atgccgtgga 2340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac 2400

2341 gctggggttt tttgaactaa acccactacc aagtgcatca cccggtagcg ggactatctg 2400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac 2460

2401 ccaaaaagcg ggaaactgca acctcaggtg caagaaatta tcacctgaga acaaggtttg 2460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat 2520

2461 accttgttgt gagttgggat agagcccgat aagaaaacta aatattccct aaaacggcta 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa 2580

2521 aagccggata accaattttt tactcgacta aattgttttt aaattgcgct taaaattgtt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata 2640

2581 ttataattgc aaatgttaaa ataccacgtg agagtcatgt tagacgagac tacggcgtat 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gttaagccaa ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca 2700

2641 caattcggtt gaggcgatag cgatgcactg acccagtacc gacgcggggc tgtgggcggt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 2760

2701 tgtgggcgac tgcgcgggac tgcccgaaca gacgagggcc gtaggcgaat gtctgttcga 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 2820

2761 cactggcaga ggccctcgac gtacacagtc tccaaaagtg gcagtagtgg ctttgcgcgc 2820

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aggcagtatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 2880

2821 tccgtcataa gaacttctgc tttcccggag cactatgcgg ataaaaatat ccaattacag 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 2940

2881 tactattatt accaaagaat ctgcagtcca ccgtgaaaag cccctttaca cgcgccttgg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 3000

2941 ggataaacaa ataaaaagat ttatgtaagt ttatacatag gcgagtactc tgttattggg 3000

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

3001 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 3060

3001 actatttacg aagttattat aactttttcc ttctcatact cataagttgt aaaggcacag 3060

.......... .......... .......... ........ Amp_Res ORF 1 ........

.......... .......... .......... .......... .......... ..........

3061 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 3120

3061 cgggaataag ggaaaaaacg ccgtaaaacg gaaggacaaa aacgagtggg tctttgcgac 3120

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 3180

3121 cactttcatt ttctacgact tctagtcaac ccacgtgctc acccaatgta gcttgaccta 3180

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 3240

3181 gagttgtcgc cattctagga actctcaaaa gcggggcttc ttgcaaaagg ttactactcg 3240

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acttttaaag ttctgctatg tggcgcggta ttatcccgtg atgacgccgg gcaagagcaa 3300

3241 tgaaaatttc aagacgatac accgcgccat aatagggcac tactgcggcc cgttctcgtt 3300

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... ScaI(3344) ..........

3301 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 3360

3301 gagccagcgg cgtatgtgat aagagtctta ctgaaccaac tcatgagtgg tcagtgtctt 3360

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 3420

3361 ttcgtagaat gcctaccgta ctgtcattct cttaatacgt cacgacggta ttggtactca 3420

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 3480

3421 ctattgtgac gccggttgaa tgaagactgt tgctagcctc ctggcttcct cgattggcga 3480

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 3540

3481 aaaaacgtgt tgtaccccct agtacattga gcggaactag caacccttgg cctcgactta 3540

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gaagccatac caaacgacga gcgtgacacc acgatgccag cagcaatggc aacaacgttg 3600

3541 cttcggtatg gtttgctgct cgcactgtgg tgctacggtc gtcgttaccg ttgttgcaac 3600

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 3660

3601 gcgtttgata attgaccgct tgatgaatga gatcgaaggg ccgttgttaa ttatctgacc 3660

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 3720

3661 tacctccgcc tatttcaacg tcctggtgaa gacgcgagcc gggaaggccg accgaccaaa 3720

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 3780

3721 taacgactat ttagacctcg gccactcgca cccagagcgc catagtaacg tcgtgacccc 3780

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 3840

3781 ggtctaccat tcgggagggc atagcatcaa tagatgtgct gcccctcagt ccgttgatac 3840

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 3900

3841 ctacttgctt tatctgtcta gcgactctat ccacggagtg actaattcgt aaccattgac 3900

.......... ........ Amp_Res ORF 1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 3960

3901 agtctggttc aaatgagtat atatgaaatc taactaaatt ttgaagtaaa aattaaattt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 4020

3961 tcctagatcc acttctagga aaaactatta gagtactggt tttagggaat tgcactcaaa 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 4080

4021 agcaaggtga ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa 4080

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4081 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 4140

4081 aaagacgcgc attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 4200

4141 aacggcctag ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 4260

4201 tatggtttat gacaggaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 4320

4261 cgtggcggat gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 4380

4321 ttcagcacag aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4440

4381 ccgacttgcc ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag aaaggcggac 4500

4441 tctatggatg tcgcactcgt aactctttcg cggtgcgaag ggcttccctc tttccgcctg 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4560

4501 tccataggcc attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4620

4561 ttgcggacca tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 4680

4621 aacactacga gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 4740

4681 gccaaggacc ggaaaacgac cggaaaacga gtgtacaaga aaggacgcaa taggggacta 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 4800

4741 agacacctat tggcataatg gcggaaactc actcgactat ggcgagcggc gtcggcttgc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct 4860

4801 tggctcgcgt cgctcagtca ctcgctcctt cgccttctcg cgggttatgc gtttggcgga 4860

.......... .......... .......... .......... .......... ..........

.......... .......... ......... PvuII(4893) ......... ..........

4861 ctccccgcgc gttggccgat tcattaatcc agctggcacg acaggtttcc cgactggaaa 4920

4861 gaggggcgcg caaccggcta agtaattagg tcgaccgtgc tgtccaaagg gctgaccttt 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcgggcagtg agcgcaacgc aattaatgtg agttacctca ctcattaggc accccaggct 4980

4921 cgcccgtcac tcgcgttgcg ttaattacac tcaatggagt gagtaatccg tggggtccga 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac 5040

4981 aatgtgaaat acgaaggccg agcatacaac acaccttaac actcgcctat tgttaaagtg 5040

......... lac_promoter ......... .......... ...... M13_pUC_rev_primer

.......... .......... .......... .......... .......... ..........

5041 acaggaaaca gctatgacca tgattacgaa ttaa...... .......... .......... 5074

5041 tgtcctttgt cgatactggt actaatgctt aatt...... .......... .......... 5074

...... M13_reverse_primer ...... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-5074 Reference author: Wilson P.C.; Reference title: "Ig-kappa expression vector for expression-cloning of antibody variable Reference title: region genes"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-5074 Reference author: Wilson P.C., Wilson P.C., Wilson P.C.; Reference title: ; Reference location: Submitted (18-NOV-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Medicine/Rheumatology, The University of Chicago, BSLC/JFK Bldg. 924 East Reference location: 57th St., Rm 414, Chicago, IL 60637, USA