Adenoviral Plasmids
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pAdTrack
Type of vector: Mammalian expression,Adenoviral
Backbone size (bp): 8320
Bacteria resistance: Kanamycin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: Yes
Principal Investigator: Bert Vogelstein
Additional Documents: AdEasy Protocol
Comments: Vector pAdTrack is used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.
Max insert size = 5.9kb with pAdEasy1 and 8.6kb with pAdEasy2. < br>Article: A simplified system for generating recombinant adenoviruses. He TC et al. (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. Pubmed)
pShuttle
GenBank/Entrez ID of insert: AF334399
Vector backbone: pShuttle
Type of vector: Mammalian expression,Adenoviral
Backbone size (bp): 6736
Bacteria resistance: Kanamycin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: Yes
Comments: Vector pShuttle is used for expression of transgenes under a chosen (non-CMV) promoter when no GFP tracer is desired.
Max insert size = 7.5kb with pAdEasy1 and 10.2kb with pAdEasy2.
Article: A simplified system for generating recombinant adenoviruses. He TC et al. (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. Pubmed)
pShuttle-CMV
GenBank/Entrez ID of insert: AF334399
Vector backbone: pShuttle-CMV
Type of vector: Mammalian expression,Adenoviral
Backbone size (bp): 7452
5' Sequencing primer: CMV-F (List of Sequencing Primers)
Bacteria resistance: Kanamycin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH10B
Principal Investigator: Bert Vogelstein
Comments: Vector pShuttle-CMV is used for expression of transgenes under a CMV promoter when no GFP tracer is desired. Max insert size = 6.6kb with pAdEasy1 and 9.3kb with pAdEasy2.
Article: A simplified system for generating recombinant adenoviruses. He TC et al. (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. Pubmed)
pAdTrack-CMV
Vector backbone: pAdTrack-CMV
Type of vector: Mammalian expression,Adenoviral
Backbone size (bp): 9220
5' Sequencing primer: n/a (List of Sequencing Primers)
Bacteria resistance: Kanamycin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH10B
Principal Investigator: Bert Vogelstein
Additional Documents: AdEasy Protocol (PDF)
Comments: Vector pAdTrack-CMV is used for expression of transgenes under a CMV promoter when a GFP tracer is desired.
Max insert size = 5.0kb with pAdEasy1 and 7.7kb with pAdEasy2.
Article: A simplified system for generating recombinant adenoviruses. He TC et al. (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. Pubmed)
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