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pAAV-Syn-AlstR-IRES2-EGFP - Gene/insert name: AlstR; Alternative names: allatostatin receptor; Species of gene(s): D. melanogaster (fly); Vector backbone: pAAV-Syn-IRES2-EGFP (Search Vector Database); 5' Sequencing primer: pBS-KS (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Plasmid Provided I...
pACAGW-ChR2-Venus-AAV - Gene/insert name: Channelrhodopsin-2; Alternative names: ChR2; Channelrhodopsin; Gene/insert aliases: COP4; Species of gene(s): Chlamydomonas reinhardtii; Fusion proteins or tags: Venus; Terminal: C terminal on insert; Vector backbone: N/A (Search Vector Database); Cloning site 5': BamHI; Site destroyed during cloning: No; Cloning site 3': EcoRI; Sit...
pAdEasy-1 - Gene/insert name: none; GenBank/Entrez ID of insert: AY370909; Vector backbone: pAdEasy-1 (Search Vector Database); 5' Sequencing primer: n/a (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: Low Copy; Grow in standard E. coli @ 37C: Yes; Plasmid Provided In: DH10B; Principal Investigator: Bert Vogelstein; Comments: Recombine plasmids from the shut...
pAdEasy-2 - Gene/insert name: none; GenBank/Entrez ID of insert: AY370909; Vector backbone: pAdEasy-2 (Search Vector Database); 5' Sequencing primer: n/a (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: Low Copy; Grow in standard E. coli @ 37C: Yes; Plasmid Provided In: DH10B; Principal Investigator: Bert Vogelstein; Comments: Recombine plasmids from the shut...
pAdTrack -
Bacteria resistance: Kanamycin;
High or low copy: Low Copy ;
Grow in standard E. coli @ 37C: Yes;
Principal Investigator: Bert Vogelstein ;
Additional Documents: AdEasy Protocol ;
Comments: Vector pAdTrack is used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.
Max insert size = 5.9kb with pAdEasy1 and 8.6kb with pAdEasy2. < b...
Vector backbone: pAdTrack-CMV -
5' Sequencing primer: n/a (List of Sequencing Primers) ;
Bacteria resistance: Kanamycin ;
High or low copy: Low Copy ;
Grow in standard E. coli @ 37C: Yes;
Plasmid Provided In: DH10B ;
Principal Investigator: Bert Vogelstein ;
Additional Documents: AdEasy Protocol (PDF); Article: A simplified system for generating recombinant adenoviruses. H...
pShuttle - GenBank/Entrez ID of insert: AF334399 ;
Vector backbone: pShuttle;
Bacteria resistance: Kanamycin;
High or low copy: Low Copy ;
Grow in standard E. coli @ 37C: Yes; Article: A simplified system for generating recombinant adenoviruses. He TC et al. (Proc Natl Acad Sci U S A. 1998 Mar 3. 95(5):2509-14. Pubmed)
pShuttle-CMV - GenBank/Entrez ID of insert: AF334399 ;
Vector backbone: pShuttle-CMV ;
5' Sequencing primer: CMV-F (List of Sequencing Primers) ;
Bacteria resistance: Kanamycin ;
High or low copy: Low Copy ;
Grow in standard E. coli @ 37C: Yes;
Plasmid Provided In: DH10B ;
Principal Investigator: Bert Vogelstein; Article: A simplified system for generating recombinant a...
pShuttle-CMV-EGFP-C - Gene/insert name: C domain of N-WASP; Alternative names: EGFP-C; WASL; GenBank/Entrez ID of insert: NM_174219; Gene/insert aliases: WASL, WASL; Species of gene(s): B. taurus (bovine); Relevant mutations/deletions: The BglII site in the MCS of pShuttle-CMV was filled and the vector was religated in order to remove this BglII site.; Fusion proteins or tags: EGFP;...
pShuttle-CMV-EGFP-CA - Gene/insert name: CA domains of N-WASP; Alternative names: EGFP-CA; WASL; GenBank/Entrez ID of insert: NM_174219; Gene/insert aliases: WASL, WASL; Species of gene(s): B. taurus (bovine); Relevant mutations/deletions: The BglII site in the MCS of pShuttle-CMV was filled and the vector was religated in order to remove this BglII site.; Fusion proteins or tags: ...
