{]{]{1 tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccg 51 gagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccg 101 tcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatg 151 cggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaata 201 ccgcacagatgcgtaaggagaaaataccgcatcaggcgacgcgccctgta 251 gcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgct 301 acacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctt 351 tctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctcc 401 ctttagggttccgatttagtgctttacggcacctcgaccccaaaaaactt 451 gattagggtgatggttcacgtagtgggccatcgccctgatagacggtttt 501 tcgccctttgacgttggagtccacgttctttaatagtggactcttgttcc 551 aaactggaacaacactcaaccctatctcggtctattcttttgatttataa 601 gggattttgccgatttcggcctattggttaaaaaatgagctgatttaaca 651 aaaatttaacgcgaattttaacaaaatattaacgtttacaatttcgccat 701 tcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctc 751 ttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaa 801 gttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggcc 851 agtgaattgtaatacgactcactatagggcgaattcgagctcggtacccg 901 gggatcctctagagccacccccctggtgcaggcatgcaagcttttgttcc 951 ctttagtgagggttaattccgagcttggcgtaatcatggtcatagctgtt 1001 tcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccg 1051 gaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcaca 1101 ttaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtg 1151 ccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgta 1201 ttgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgtt 1251 cggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatc 1301 cacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagc 1351 aaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccatagg 1401 ctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtg 1451 gcgaaacccgacaggactataaagataccaggcgtttccccctggaagct 1501 ccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtcc 1551 gcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtag 1601 gtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacg 1651 aaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtctt 1701 gagtccaacccggtaagacacgacttatcgccactggcagcagccactgg 1751 taacaggattagcagagcgaggtatgtaggcggtgctacagagttcttga 1801 agtggtggcctaactacggctacactagaaggacagtatttggtatctgc 1851 gctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatc 1901 cggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagc 1951 agattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttct 2001 acggggtctgacgctcagtggaacgaaaactcacgttaagggattttggt 2051 catgagattatcaaaaaggatcttcacctagatccttttaaattaaaaat 2101 gaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagt 2151 taccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcg 2201 ttcatccatagttgcctgactccccgtcgtgtagataactacgatacggg 2251 agggcttaccatctggccccagtgctgcaatgataccgcgagacccacgc 2301 tcaccggctccagatttatcagcaataaaccagccagccggaagggccga 2351 gcgcagaagtggtcctgcaactttatccgcctccatccagtctattaatt 2401 gttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaac 2451 gttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtat 2501 ggcttcattcagctccggttcccaacgatcaaggcgagttacatgatccc 2551 ccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtc 2601 agaagtaagttggccgcagtgttatcactcatggttatggcagcactgca 2651 taattctcttactgtcatgccatccgtaagatgcttttctgtgactggtg 2701 agtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgc 2751 tcttgcccggcgtcaatacgggataataccgcgccacatagcagaacttt 2801 aaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaagga 2851 tcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaac 2901 tgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaac 2951 aggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgtt 3001 gaatactcatactcttcctttttcaatattattgaagcatttatcagggt 3051 tattgtctcatgagcggatacatatttgaatgtatttagaaaaataaaca 3101 aataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaag 3151 aaaccattattatcatgacattaacctataaaaataggcgtatcacgagg 3201 ccctttcgtc BSB- "Times New Roman{]C 3 pGEX_3_primer"Times New Roman{]C 2 f1_origin"Times New Roman{]CɆ XlacZ_a"Times New Roman{] 6L M13_pUC_f"Times New Roman{]C EUM13_f20"Times New Roman3{]C \nT7_pro"Times New Roman{]CɆ T3_pro"Times New Roman{] M13_r"Times New Roman{]C  M13_pUC_r"Times New Roman{]C + lac_promoter"Times New Roman{]CɆ ` pBR322_origin"Times New Roman{] f  Orf frame 2"Times New Roman<{]C f  Ampicillin"Times New Roman{]C   AmpR_promoter"Times New Roman {]NdeI"Times New Roman   } {]PEcoRI(881), SacI(891), KpnI(897), SmaI(899), BamHI(902), XbaI(908), HindIII(938)"Times New Roman/ Ym {]AatII"Times New RomanI  J:D~#oe~ {]test"Times New Roman W/&&