Sequence of BSBMinus

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Sequence of BSBMinus

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Sequence of BSB- : 3210 BP; 776 A; 813 C; 803 G; 818 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60

1 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 60

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120

61 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180

121 aaccgcccac agccccgacc gaattgatac gccgtagtct cgtctaacat gactctcacg 180

.......... .......... .......... .......... .......... ..........

NdeI(184) .......... .......... .......... .......... ..........

181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgac 240

181 tggtatacgc cacactttat ggcgtgtcta cgcattcctc ttttatggcg tagtccgctg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 300

241 cgcgggacat cgccgcgtaa ttcgcgccgc ccacaccacc aatgcgcgtc gcactggcga 300

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

301 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 360

301 tgtgaacggt cgcgggatcg cgggcgagga aagcgaaaga agggaaggaa agagcggtgc 360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 420

361 aagcggccga aaggggcagt tcgagattta gcccccgagg gaaatcccaa ggctaaatca 420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 480

421 cgaaatgccg tggagctggg gttttttgaa ctaatcccac taccaagtgc atcacccggt 480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 540

481 agcgggacta tctgccaaaa agcgggaaac tgcaacctca ggtgcaagaa attatcacct 540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 600

541 gagaacaagg tttgaccttg ttgtgagttg ggatagagcc agataagaaa actaaatatt 600

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 660

601 ccctaaaacg gctaaagccg gataaccaat tttttactcg actaaattgt ttttaaattg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gcgaatttta acaaaatatt aacgtttaca atttcgccat tcgccattca ggctgcgcaa 720

661 cgcttaaaat tgttttataa ttgcaaatgt taaagcggta agcggtaagt ccgacgcgtt 720

.......... .......... .......... .......... .......... lacZ_a

.......... .......... .......... .......... .......... ..........

721 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 780

721 gacaaccctt cccgctagcc acgcccggag aagcgataat gcggtcgacc gctttccccc 780

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 840

781 tacacgacgt tccgctaatt caacccattg cggtcccaaa agggtcagtg ctgcaacatt 840

.......... .......... lacZ_a .......... .......... M13_pUC_f

.......... .......... .......... .......... EcoRI(881) SacI(891)

841 aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc tcggtacccg 900

841 ttgctgccgg tcacttaaca ttatgctgag tgatatcccg cttaagctcg agccatgggc 900

lacZ_a M13_f20 ...... T7_pro .......... .......... ..........

BamHI(902) XbaI(908) ......... HindIII(938) ......... ..........

901 gggatcctct agagccaccc ccctggtgca ggcatgcaag cttttgttcc ctttagtgag 960

901 ccctaggaga tctcggtggg gggaccacgt ccgtacgttc gaaaacaagg gaaatcactc 960

.......... .......... .......... .......... .......... T3_pro

.......... .......... .......... .......... .......... ..........

961 ggttaattcc gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc 1020

961 ccaattaagg ctcgaaccgc attagtacca gtatcgacaa aggacacact ttaacaatag 1020

.......... .......... .......... M13_r .......... M13_pUC_r

.......... .......... .......... .......... .......... ..........

1021 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 1080

1021 gcgagtgtta aggtgtgttg tatgctcggc cttcgtattt cacatttcgg accccacgga 1080

.......... .......... ......... lac_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

1081 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 1140

1081 ttactcactc gattgagtgt aattaacgca acgcgagtga cgggcgaaag gtcagccctt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 1200

1141 tggacagcac ggtcgacgta attacttagc cggttgcgcg cccctctccg ccaaacgcat 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 1260

1201 aacccgcgag aaggcgaagg agcgagtgac tgagcgacgc gagccagcaa gccgacgccg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 1320

1261 ctcgccatag tcgagtgagt ttccgccatt atgccaatag gtgtcttagt cccctattgc 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 1380

1321 gtcctttctt gtacactcgt tttccggtcg ttttccggtc cttggcattt ttccggcgca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 1440

1381 acgaccgcaa aaaggtatcc gaggcggggg gactgctcgt agtgttttta gctgcgagtt 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 1500

1441 cagtctccac cgctttgggc tgtcctgata tttctatggt ccgcaaaggg ggaccttcga 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 1560

1501 gggagcacgc gagaggacaa ggctgggacg gcgaatggcc tatggacagg cggaaagagg 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 1620

1561 gaagcccttc gcaccgcgaa agagtatcga gtgcgacatc catagagtca agccacatcc 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 1680

1621 agcaagcgag gttcgacccg acacacgtgc ttggggggca agtcgggctg gcgacgcgga 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 1740

1681 ataggccatt gatagcagaa ctcaggttgg gccattctgt gctgaatagc ggtgaccgtc 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 1800

1741 gtcggtgacc attgtcctaa tcgtctcgct ccatacatcc gccacgatgt ctcaagaact 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 1860

1801 tcaccaccgg attgatgccg atgtgatctt cctgtcataa accatagacg cgagacgact 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 1920

1861 tcggtcaatg gaagcctttt tctcaaccat cgagaactag gccgtttgtt tggtggcgac 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 1980

1921 catcgccacc aaaaaaacaa acgttcgtcg tctaatgcgc gtcttttttt cctagagttc 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 2040

1981 ttctaggaaa ctagaaaaga tgccccagac tgcgagtcac cttgcttttg agtgcaattc 2040

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 2100

2041 cctaaaacca gtactctaat agtttttcct agaagtggat ctaggaaaat ttaattttta 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 2160

2101 cttcaaaatt tagttagatt tcatatatac tcatttgaac cagactgtca atggttacga 2160

.......... .......... .......... .......... .... Orf frame 2 Ampicillin

.......... .......... .......... .......... .......... ..........

2161 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 2220

2161 attagtcact ccgtggatag agtcgctaga cagataaagc aagtaggtat caacggactg 2220

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 2280

2221 aggggcagca catctattga tgctatgccc tcccgaatgg tagaccgggg tcacgacgtt 2280

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 2340

2281 actatggcgc tctgggtgcg agtggccgag gtctaaatag tcgttatttg gtcggtcggc 2340

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 2400

2341 cttcccggct cgcgtcttca ccaggacgtt gaaataggcg gaggtaggtc agataattaa 2400

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 2460

2401 caacggccct tcgatctcat tcatcaagcg gtcaattatc aaacgcgttg caacaacggt 2460

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 2520

2461 aacgatgtcc gtagcaccac agtgcgagca gcaaaccata ccgaagtaag tcgaggccaa 2520

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 2580

2521 gggttgctag ttccgctcaa tgtactaggg ggtacaacac gttttttcgc caatcgagga 2580

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 2640

2581 agccaggagg ctagcaacag tcttcattca accggcgtca caatagtgag taccaatacc 2640

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 2700

2641 gtcgtgacgt attaagagaa tgacagtacg gtaggcattc tacgaaaaga cactgaccac 2700

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 2760

2701 tcatgagttg gttcagtaag actcttatca catacgccgc tggctcaacg agaacgggcc 2760

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 2820

2761 gcagttatgc cctattatgg cgcggtgtat cgtcttgaaa ttttcacgag tagtaacctt 2820

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 2880

2821 ttgcaagaag ccccgctttt gagagttcct agaatggcga caactctagg tcaagctaca 2880

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 2940

2881 ttgggtgagc acgtgggttg actagaagtc gtagaaaatg aaagtggtcg caaagaccca 2940

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 3000

2941 ctcgtttttg tccttccgtt ttacggcgtt ttttccctta ttcccgctgt gcctttacaa 3000

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 3060

3001 cttatgagta tgagaaggaa aaagttataa taacttcgta aatagtccca ataacagagt 3060

Orf frame 2 Ampicillin ........ .......... ........ AmpR_promoter

.......... .......... .......... .......... .......... ..........

3061 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 3120

3061 actcgcctat gtataaactt acataaatct ttttatttgt ttatccccaa ggcgcgtgta 3120

AmpR_promoter ...... .......... .......... .......... ..........

.......... ......... AatII(3145) ......... .......... ..........

3121 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 3180

3121 aaggggcttt tcacggtgga ctgcagattc tttggtaata atagtactgt aattggatat 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aaaataggcg tatcacgagg ccctttcgtc .......... .......... .......... 3210

3181 ttttatccgc atagtgctcc gggaaagcag .......... .......... .......... 3210

.......... .......... .......... .......... .......... ..........