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Backbone Size (bp): 3210
Catalog Number: 67724
Comments: Created by Moore, July 1995, under contract with NCBI. BSB+ (away from cloning site) and BSB- (toward cloning site) differ in their orientation of the intergenic region from bacteriophage f1. This vector contains the integenic region from bacteriophage f1 permitting synthesis of single-strand copies of the insert. A plasmid vector containing convenient priming sites for sequence analysis and synthesis of RNA in either direction from T3 and T7 promoters. Constructed from Bluescribe M13+ by insertion of a synthetic BstXI adapter between the PstI and XbaI sites of the polylinker region. Restriction digests of the clone give the following sizes (kb): NdeI/HindIII--2.5, 0.7; NdeI--3.2; PstI--uncut; BamHI--3.2; PvuII--2.7, 0.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, E.coli. Related vectors: BlueScribe M13+. (Information source: VectorDB.)