kk~ 1 ctgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacg 51 cgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgc 101 tttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctc 151 taaatcgggggctccctttagggttccgatttagtgctttacggcacctc 201 gaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgcc 251 ctgatagacggtttttcgccctttgacgttggagtccacgttctttaata 301 gtggactcttgttccaaactggaacaacactcaaccctatctcggtctat 351 tcttttgatttataagggattttgccgatttcggcctattggttaaaaaa 401 tgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgc 451 ttacaatttccattcgccattcaggctgcgcaactgttgggaagggcgat 501 cggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgct 551 gcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttg 601 taaaacgacggccagtgagcgcgcgtaatacgactcactatagggcgaat 651 tggagctccaccgcggtggcggccgctctagaactagtggatcccccggg 701 ctgcaggaattcggactgtgtgtcagatctgactaagggtcggaattcga 751 tatcaagcttatcgataccgtcgacctcgagggggggcccggtacccagc 801 ttttgttccctttagtgagggttaattgcgcgcttggcgtaatcatggtc 851 atagctgtttcctgtgtgaaattgttatccgctcacaattccacacaaca 901 tacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagc 951 taactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaa 1001 cctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcg 1051 gtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgc 1101 tcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaat 1151 acggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaa 1201 aaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgttt 1251 ttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaag 1301 tcagaggtggcgaaacccgacaggactataaagataccaggcgtttcccc 1351 ctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgga 1401 tacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctc 1451 acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggct 1501 gtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaac 1551 tatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagc 1601 agccactggtaacaggattagcagagcgaggtatgtaggcggtgctacag 1651 agttcttgaagtggtggcctaactacggctacactagaaggacagtattt 1701 ggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtag 1751 ctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgttt 1801 gcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttg 1851 atcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagg 1901 gattttggtcatgagattatcaaaaaggatcttcacctagatccttttaa 1951 attaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttgg 2001 tctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctg 2051 tctatttcgttcatccatagttgcctaaatccccaaagtgtagataacta 2101 cgatacgggagggcttaccatctggccccagtgctgcaatgataccgcga 2151 gacccacgctcaccggctccagatttatcagcaataaaccagccagccgg 2201 aagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagt 2251 ctattaattgttgccgggaagctagagtaagtagttcgccagttaatagt 2301 ttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtc 2351 gtttggtatggcttcattcagctccggttcccaacgatcaaggcgagtta 2401 catgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccg 2451 atcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggc 2501 agcactgcataattctcttactgtcatgccatccgtaagatgcttttctg 2551 tgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcga 2601 ccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatag 2651 cagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaac 2701 tctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgt 2751 gcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtg 2801 agcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacac 2851 ggaaatgttgaatactcatactcttcctttttcaatattattgaagcatt 2901 tatcagggttattgtctcatgagcggatacatatttgaatgtatttagaa 2951 aaataaacaaataggggttccgcgcacatttccccgaaaagtgccac BSII TKS- "Times New RomankC G f1_origin"Times New RomankC flacZ_a"Times New RomankCɆ I_ M13_pUC_f"Times New Romank XhM13_f20"Times New Roman3kC rT7_pro"Times New RomankC pBluescriptSK_primer"Times New RomankCɆ  pBluescriptKS_primer"Times New Romank (;T3_pro"Times New RomankC L^M13_r"Times New RomankC ]s M13_pUC_r"Times New RomankCɆ lac_pro"Times New Romank > pBR322_origin"Times New RomankC 5  Ampicillin"Times New Roman<kC 5  Orf frame 2"Times New RomankCɆ _ {  AmpR_promoter"Times New Roman kSacI(657), SacII(664), NotI(670), EagI(670), XbaI(677), SpeI(683), BamHI(689), SmaI(697), PstI(705), BglII(725), EcoRV(751), HindIII(755), ClaI(762), SalI(770), XhoI(776), ApaI(789), KpnI(795) "Times New Roman h|6 ktest"Times New Roman iO8B8