Sequence of BSIITKSMinus

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Sequence of BSIITKSMinus

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Sequence of BSII TKS- : 2997 BP; 719 A; 762 C; 745 G; 771 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 60

1 gactgcgcgg gacatcgccg cgtaattcgc gccgcccaca ccaccaatgc gcgtcgcact 60

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 120

61 ggcgatgtga acggtcgcgg gatcgcgggc gaggaaagcg aaagaaggga aggaaagagc 120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 180

121 ggtgcaagcg gccgaaaggg gcagttcgag atttagcccc cgagggaaat cccaaggcta 180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 240

181 aatcacgaaa tgccgtggag ctggggtttt ttgaactaat cccactacca agtgcatcac 240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 300

241 ccggtagcgg gactatctgc caaaaagcgg gaaactgcaa cctcaggtgc aagaaattat 300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 360

301 cacctgagaa caaggtttga ccttgttgtg agttgggata gagccagata agaaaactaa 360

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 420

361 atattcccta aaacggctaa agccggataa ccaatttttt actcgactaa attgttttta 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 480

421 aattgcgctt aaaattgttt tataattgcg aatgttaaag gtaagcggta agtccgacgc 480

.......... .......... .......... .......... .......... lacZ_a

.......... .......... .......... .......... .......... ..........

481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540

481 gttgacaacc cttcccgcta gccacgcccg gagaagcgat aatgcggtcg accgctttcc 540

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600

541 ccctacacga cgttccgcta attcaaccca ttgcggtccc aaaagggtca gtgctgcaac 600

.......... .......... lacZ_a .......... .......... M13_pUC_f

.......... .......... .......... .......... .......... ..........

601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 660

601 attttgctgc cggtcactcg cgcgcattat gctgagtgat atcccgctta acctcgaggt 660

lacZ_a M13_f20 ...... .......... T7_pro .......... ..........

SacII NotI,EagI XbaI SpeI83) BamHI(689) SmaI(697)

661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcggactgtg 720

661 ggcgccaccg ccggcgagat cttgatcacc tagggggccc gacgtcctta agcctgacac 720

.......... ..... pBluescriptSK_primer ..... .......... ..........

BglII ........ EcoRV(751),HindIII(755) ClaI(762) SalI(770)

721 tgtcagatct gactaagggt cggaattcga tatcaagctt atcgataccg tcgacctcga 780

721 acagtctaga ctgattccca gccttaagct atagttcgaa tagctatggc agctggagct 780

.......... .......... .......... .......... ..... pBluescriptKS_primer

ApaI(789) KpnI(795) .......... .......... .......... ..........

781 gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt 840

781 cccccccggg ccatgggtcg aaaacaaggg aaatcactcc caattaacgc gcgaaccgca 840

.......... .......... .......... T3_pro .......... ..........

.......... .......... .......... .......... .......... ..........

841 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 900

841 ttagtaccag tatcgacaaa ggacacactt taacaatagg cgagtgttaa ggtgtgttgt 900

.......... M13_r .......... M13_pUC_r .......... ..........

.......... .......... .......... .......... .......... ..........

901 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 960

901 atgctcggcc ttcgtatttc acatttcgga ccccacggat tactcactcg attgagtgta 960

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 1020

961 attaacgcaa cgcgagtgac gggcgaaagg tcagcccttt ggacagcacg gtcgacgtaa 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 1080

1021 ttacttagcc ggttgcgcgc ccctctccgc caaacgcata acccgcgaga aggcgaagga 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 1140

1081 gcgagtgact gagcgacgcg agccagcaag ccgacgccgc tcgccatagt cgagtgagtt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 1200

1141 tccgccatta tgccaatagg tgtcttagtc ccctattgcg tcctttcttg tacactcgtt 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 1260

1201 ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg 1260

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1261 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 1320

1261 aggcgggggg actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 1380

1321 gtcctgatat ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 1440

1381 gctgggacgg cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 1500

1441 gagtatcgag tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 1560

1501 cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 1620

1561 tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 1680

1621 cgtctcgctc catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 1740

1681 tgtgatcttc ctgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 1800

1741 ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 1860

1801 cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 1920

1861 gccccagact gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tactctaata 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 1980

1921 gtttttccta gaagtggatc taggaaaatt taatttttac ttcaaaattt agttagattt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 2040

1981 catatatact catttgaacc agactgtcaa tggttacgaa ttagtcactc cgtggataga 2040

.......... .......... .......... .......... Ampicillin ..........

.......... .......... .......... .......... .......... ..........

2041 cagcgatctg tctatttcgt tcatccatag ttgcctaaat ccccaaagtg tagataacta 2100

2041 gtcgctagac agataaagca agtaggtatc aacggattta ggggtttcac a tctattgat 2100

.......... .......... Ampicillin ......... Orf frame 2 .........

.......... .......... .......... .......... .......... ..........

2101 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 2160

2101 gctatgccct cccgaatggt agaccggggt cacgacgtta ctatggcgct ctgggtgcga 2160

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 2220

2161 gtggccgagg tctaaatagt cgttatttgg tcggtcggcc ttcccggctc gcgtcttcac 2220

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 2280

2221 caggacgttg aaataggcgg aggtaggtca gataattaac aacggccctt cgatctcatt 2280

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 2340

2281 catcaagcgg tcaattatca aacgcgttgc aacaacggta acgatgtccg tagcaccaca 2340

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 2400

2341 gtgcgagcag caaaccatac cgaagtaagt cgaggccaag ggttgctagt tccgctcaat 2400

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 2460

2401 gtactagggg gtacaacacg ttttttcgcc aatcgaggaa gccaggaggc tagcaacagt 2460

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 2520

2461 cttcattcaa ccggcgtcac aatagtgagt accaataccg tcgtgacgta ttaagagaat 2520

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 2580

2521 gacagtacgg taggcattct acgaaaagac actgaccact catgagttgg ttcagtaaga 2580

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 2640

2581 ctcttatcac atacgccgct ggctcaacga gaacgggccg cagttatgcc ctattatggc 2640

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 2700

2641 gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt tgcaagaagc cccgcttttg 2700

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 2760

2701 agagttccta gaatggcgac aactctaggt caagctacat tgggtgagca cgtgggttga 2760

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 2820

2761 ctagaagtcg tagaaaatga aagtggtcgc aaagacccac tcgtttttgt ccttccgttt 2820

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 2880

2821 tacggcgttt tttcccttat tcccgctgtg cctttacaac ttatgagtat gagaaggaaa 2880

.......... .... Ampicillin Orf frame 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 2940

2881 aagttataat aacttcgtaa atagtcccaa taacagagta ctcgcctatg tataaactta 2940

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

2941 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac... 2997

2941 cataaatctt tttatttgtt tatccccaag gcgcgtgtaa aggggctttt cacggtg... 2997

.......... .......... .......... .......... .......... ..........