zRzRPID L08784; SV 1; circular; genomic DNA; STD; SYN; 2964 BP. AC L08784; DT 10-OCT-1993 (Rel. 37, Created) DT 04-MAR-2000 (Rel. 63, Last updated, Version 2) DE BlueScribe KS Minus cloning vector. KW . OS synthetic construct OC other sequences; artificial sequences. RN [1] RP 1-2964 RA Gilbert W.; RT "Obtained from VecBase 3.0"; RL Unpublished. CC These data and their annotation were supplied to GenBank by Will CC Gilbert under the auspices of the GenBank Currator Program. CC BlueScribe KS Minus - Cloning vector CC ENTRY BLUEKSM #TYPE DNA CIRCULAR CC TITLE BlueScribe KS Minus - Cloning vector CC DATE 28-JAN-1987 CC #sequence 02-FEB-1987 CC #sequence 04-MAR-1987 CC #sequence 03-APR-1987 CC ACCESSION VB0077 CC SOURCE artificial CC REFERENCE CC #number 1 CC #authors Fernandez J.M., Short J.M., Renshaw M., Huse W., Sorge CC J. CC #journal Gene (1987) in press CC #citation Sequence data from StrataGene CC #comment sequence correction according to Stratagene COMMENT CC Obtained from StrataGene on floppy disc. CC Revised 02-FEB-1987 by F. Pfeiffer: CC 1409/10 'AT' to 'TA' to match revised sequence of PBR322 CC Revised 4-MAR-1987 to match sequence of pUC19 on request CC of Stratagene CC Polylinker region revised 03-APR-1987 according to Stratagene CC COMMENT CC The stand shown corresponds to pUC19c. CC As in the published sequence of pUC19c, The M13mp19 lacZ region CC is on the complementary strand. CC COMMENT CC This vector contains the f1 origin so that the minus strand CC can be obtained upon f1 superinfection. CC KEYWORDS CC CROSSREFERENCE CC #parent CC VecBase(3):BlueM13m CC #parent CC VecBase(3):pUC19, VecSource(3):PromT7, VecSource(3):PromT3, CC VecSource(3):bGalKS, GenBank(50):PF1 CC #brother CC VecBase(3):BlueKSp, VecBase(3):BlueSKm CC PARENT CC Features of BlueKSm (2964 bp) CC residue source CC 3- 458 5488-5943 phage f1 CC 460- 624 236- 400 pUC19c CC 626- 645 1- 20 T7 promoter CC 653- 760 108- 1 (c) BlueKS-polylinker CC 772- 791 20- 1 (c) T3 promoter CC 795-2964 448-2617 pUC19c CC Conflict (cfl) and Mutations (mut): none CC PARENT CC Features of BlueKSm (2964 bp) CC residue source CC 3- 458 5488-5943 phage f1 CC 460- 624 449- 285 (c) pUC19 CC 626- 645 1- 20 T7 promoter CC 653- 760 108- 1 (c) BlueKS-polylinker CC 772- 791 20- 1 (c) T3 promoter CC 795-1031 237- 1 (c) pUC19 CC 1032-2964 2686- 754 (c) pUC19 CC Conflict (cfl) and Mutations (mut): none CC FEATURE CC 643 start of T7-RNA synthesis CC 774 (c) start of T3-RNA synthesis CC 1976-2764 789-1 (c) Ap-R; b-lactamase CC POLYLINKER CC KpnI-DraII-ApaI-XhoI-SalI-ClaI-HindIII-EcoRV-EcoRI-PstI- CC SmaI-BamHI-SpeI-XbaI-NotI-XmaIII-BstXI-SacII-SacI SELECTION CC #resistance Ap CC #indicator beta-galactosidase CC SUMMARY BlueKSm #length 2964 #checksum 1589. FH Key Location/Qualifiers FT source 1..2964 FT /organism="synthetic construct" FT /mol_type="genomic DNA" FT /db_xref="taxon:32630" SQ Sequence 2964 BP; 708 A; 756 C; 735 G; 765 T; 0 other; ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac 60 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc 120 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt 180 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg 240 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag 300 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt 360 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt 420 taacgcgaat tttaacaaaa tattaacgtt tacaatttcg cgccattcgc cattcaggct 480 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 540 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 600 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca 660 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tcgatatcaa 720 gcttatcgat accgtcgacc tcgagggggg gcccggtacc cagcttttgt tccctttagt 780 gagggttaat tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt 840 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg 900 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg 960 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc 1020 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 1080 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 1140 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 1200 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 1260 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 1320 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 1380 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 1440 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 1500 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 1560 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 1620 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 1680 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 1740 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 1800 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 1860 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 1920 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat 1980 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct 2040 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg 2100 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag 2160 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta 2220 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg 2280 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg 2340 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct 2400 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta 2460 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg 2520 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc 2580 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg 2640 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga 2700 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg 2760 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat 2820 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc 2880 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca 2940 catttccccg aaaagtgcca cctg 2964 BlueScribe KS - "Times New RomanzRC F f1_origin"Times New RomanzRC nlacZ_a"Times New RomanzRCɆ Lb M13_pUC_f"Times New RomanzR [kM13_f20"Times New Roman3zRC r T7_promoter"Times New RomanzRC pBluescriptSK_primer"Times New RomanzRCɆ pBluescriptKS_primer"Times New RomanzR  T3_promoter"Times New RomanzRC (:M13_r"Times New RomanzRC 9O M13_pUC_r"Times New RomanzRCɆ ]z lac_promoter"Times New RomanzR  pBR322_origin"Times New RomanzRC   Orf frame 2"Times New Roman<zRC   Ampicillin"Times New RomanzRCɆ ; W  AmpR_promoter"Times New Roman zRͿSacI(657), SacII(664), XbaI(677), NotI(670), EagI(670), BamHI(689), SpeI(683), SmaI(697), PstI(705), EcoRI(707), EcoRV(715), HindII(719), ClaI(726), XhoI(740), SalI(734), ApaI(753), KpnI(759)"Times New Roman Q]kkJ zRtest"Times New Romana Gn&c h_;  zRtest"Times New Roman F&