Sequence of BlueScribeM13Plus

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Sequence of BlueScribeM13Plus

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Sequence of BlueScribe M13 + : 3204 BP; 818 A; 788 C; 822 G; 776 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60

1 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 60

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120

61 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180

121 aaccgcccac agccccgacc gaattgatac gccgtagtct cgtctaacat gactctcacg 180

.......... .......... .......... .......... .......... ..........

NdeI(184) .......... .......... .......... .......... ..........

181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat 240

181 tggtatacgc cacactttat ggcgtgtcta cgcattcctc ttttatggcg tagtccttta 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt 300

241 acatttgcaa ttataaaaca attttaagcg caatttaaaa acaatttagt cgagtaaaaa 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg 360

301 attggttatc cggctttagc cgttttaggg aatatttagt tttcttatct ggctctatcc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt 420

361 caactcacaa caaggtcaaa ccttgttctc aggtgataat ttcttgcacc tgaggttgca 420

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc 480

421 gtttcccgct ttttggcaga tagtcccgct accgggtgat gcacttggta gtgggattag 480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg 540

481 ttcaaaaaac cccagctcca cggcatttcg tgatttagcc ttgggatttc cctcgggggc 540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa 600

541 taaatctcga actgcccctt tcggccgctt gcaccgctct ttccttccct tctttcgctt 600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc 660

601 tcctcgcccg cgatcccgcg accgttcaca tcgccagtgc gacgcgcatt ggtggtgtgg 660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa 720

661 gcggcgcgaa ttacgcggcg atgtcccgcg cagcgcggta agcggtaagt ccgacgcgtt 720

f1_origin .......... .......... .......... .......... lacZ_a

.......... .......... .......... .......... .......... ..........

721 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 780

721 gacaaccctt cccgctagcc acgcccggag aagcgataat gcggtcgacc gctttccccc 780

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 840

781 tacacgacgt tccgctaatt caacccattg cggtcccaaa agggtcagtg ctgcaacatt 840

.......... .......... lacZ_a .......... .......... M13_pUC_f

.......... .......... .......... .......... EcoRI(881) SacI(891)

841 aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc tcggtacccg 900

841 ttgctgccgg tcacttaaca ttatgctgag tgatatcccg cttaagctcg agccatgggc 900

lacZ_a M13_f20 ..... T7_promoter ......... .......... ..........

BamHI) XbaI(908) SalI(914) PstI(924) HindIII(932) ........

901 gggatcctct agagtcgacc tgcaggcatg caagcttttg ttccctttag tgagggttaa 960

901 ccctaggaga tctcagctgg acgtccgtac gttcgaaaac aagggaaatc actcccaatt 960

.......... .......... .......... .......... ......... T3_promoter

.......... .......... .......... .......... .......... ..........

961 ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 1020

961 aaggctcgaa ccgcattagt accagtatcg acaaaggaca cactttaaca ataggcgagt 1020

.......... .......... M13_rev ......... M13_pUC_rev .........

.......... .......... .......... .......... .......... ..........

1021 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 1080

1021 gttaaggtgt gttgtatgct cggccttcgt atttcacatt tcggacccca cggattactc 1080

.......... ......... lac_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 1140

1081 actcgattga gtgtaattaa cgcaacgcga gtgacgggcg aaaggtcagc cctttggaca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 1200

1141 gcacggtcga cgtaattact tagccggttg cgcgcccctc tccgccaaac gcataacccg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 1260

1201 cgagaaggcg aaggagcgag tgactgagcg acgcgagcca gcaagccgac gccgctcgcc 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 1320

1261 atagtcgagt gagtttccgc cattatgcca ataggtgtct tagtccccta ttgcgtcctt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 1380

1321 tcttgtacac tcgttttccg gtcgttttcc ggtccttggc atttttccgg cgcaacgacc 1380

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1381 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1440

1381 gcaaaaaggt atccgaggcg gggggactgc tcgtagtgtt tttagctgcg agttcagtct 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1500

1441 ccaccgcttt gggctgtcct gatatttcta tggtccgcaa agggggacct tcgagggagc 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1560

1501 acgcgagagg acaaggctgg gacggcgaat ggcctatgga caggcggaaa gagggaagcc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1620

1561 cttcgcaccg cgaaagagta tcgagtgcga catccataga gtcaagccac atccagcaag 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1680

1621 cgaggttcga cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1740

1681 cattgatagc agaactcagg ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1800

1741 gaccattgtc ctaatcgtct cgctccatac atccgccacg atgtctcaag aacttcacca 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 1860

1801 ccggattgat gccgatgtga tcttcctgtc ataaaccata gacgcgagac gacttcggtc 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1920

1861 aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1980

1921 caccaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 2040

1981 gaaactagaa aagatgcccc agactgcgag tcaccttgct tttgagtgca attccctaaa 2040

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 2100

2041 accagtactc taatagtttt tcctagaagt ggatctagga aaatttaatt tttacttcaa 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 2160

2101 aatttagtta gatttcatat atactcattt gaaccagact gtcaatggtt acgaattagt 2160

.......... .......... .......... .......... .... Orf frame 2 Ampicillin

.......... .......... .......... .......... .......... ..........

2161 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 2220

2161 cactccgtgg atagagtcgc tagacagata aagcaagtag gtatcaacgg actgaggggc 2220

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 2280

2221 agcacatcta ttgatgctat gccctcccga atggtagacc ggggtcacga cgttactatg 2280

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 2340

2281 gcgctctggg tgcgagtggc cgaggtctaa atagtcgtta tttggtcggt cggccttccc 2340

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 2400

2341 ggctcgcgtc ttcaccagga cgttgaaata ggcggaggta ggtcagataa ttaacaacgg 2400

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 2460

2401 cccttcgatc tcattcatca agcggtcaat tatcaaacgc gttgcaacaa cggtaacgat 2460

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2520

2461 gtccgtagca ccacagtgcg agcagcaaac cataccgaag taagtcgagg ccaagggttg 2520

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2580

2521 ctagttccgc tcaatgtact agggggtaca acacgttttt tcgccaatcg aggaagccag 2580

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2640

2581 gaggctagca acagtcttca ttcaaccggc gtcacaatag tgagtaccaa taccgtcgtg 2640

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2700

2641 acgtattaag agaatgacag tacggtaggc attctacgaa aagacactga ccactcatga 2700

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2760

2701 gttggttcag taagactctt atcacatacg ccgctggctc aacgagaacg ggccgcagtt 2760

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 2820

2761 atgccctatt atggcgcggt gtatcgtctt gaaattttca cgagtagtaa ccttttgcaa 2820

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 2880

2821 gaagccccgc ttttgagagt tcctagaatg gcgacaactc taggtcaagc tacattgggt 2880

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 2940

2881 gagcacgtgg gttgactaga agtcgtagaa aatgaaagtg gtcgcaaaga cccactcgtt 2940

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 3000

2941 tttgtccttc cgttttacgg cgttttttcc cttattcccg ctgtgccttt acaacttatg 3000

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 3060

3001 agtatgagaa ggaaaaagtt ataataactt cgtaaatagt cccaataaca gagtactcgc 3060

.......... .......... .......... .......... ........ AmpR_promoter

.......... .......... .......... .......... .......... ..........

3061 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 3120

3061 ctatgtataa acttacataa atctttttat ttgtttatcc ccaaggcgcg tgtaaagggg 3120

AmpR_promoter ...... .......... .......... .......... ..........

......... AatII(3139) ......... .......... .......... ..........

3121 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 3180

3121 cttttcacgg tggactgcag attctttggt aataatagta ctgtaattgg atatttttat 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggcgtatcac gaggcccttt cgtc...... .......... .......... .......... 3204

3181 ccgcatagtg ctccgggaaa gcag...... .......... .......... .......... 3204

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-3204 Reference author: Gilbert W.; Reference title: "Obtained from VecBase 3.0"; Reference location: Unpublished.