w’b}w’b}ÿ<ID L08782; SV 1; circular; genomic DNA; STD; SYN; 3204 BP. AC L08782; DT 10-OCT-1993 (Rel. 37, Created) DT 04-MAR-2000 (Rel. 63, Last updated, Version 2) DE BlueScribe M13 Minus cloning vector. KW . OS synthetic construct OC other sequences; artificial sequences. RN [1] RP 1-3204 RA Gilbert W.; RT "Obtained from VecBase 3.0"; RL Unpublished. CC These data and their annotation were supplied to GenBank by Will CC Gilbert under the auspices of the GenBank Currator Program. CC BlueScribe M13 Minus - Cloning vector CC ENTRY BLUEM13M #TYPE DNA CIRCULAR TITLE BlueScribe CC M13 Minus - Cloning vector CC DATE 27-MAR-1986 CC #sequence 16-DEC-1986 CC #sequence 04-MAR-1987 CC ACCESSION VB0043 CC SOURCE artificial CC REFERENCE CC #number 1 CC #authors Fernandez J.M., Short J.M., Renshaw M., Huse W., Sorge CC J. CC #journal Gene (1987) in press CC #citation Sequence data from StrataGene CC #comment sequence correction according to Stratagene COMMENT CC Obtained 28-JAN-1987 from StrataGene on floppy disc. CC Revised 2-FEB-1986 by F. Pfeiffer: CC 1580/1 'AT' to 'TA' to match revised sequence of PBR322 CC Revised 4-MAR-1987 to match sequence of pUC19 on request CC of Stratagene CC COMMENT CC The stand shown corresponds to pUC19c. CC As in the published sequence of pUC19c, The M13mp19 lacZ region CC is on the complementary strand. CC COMMENT CC Despite its name Blue M13 minus, this vector contains the CC phage f1 origin so that the minus strand can be obtained upon CC phage f1 superinfection. CC KEYWORDS CC CROSSREFERENCE CC #parent CC VecBase(3):Blue CC #parent CC VecBase(3):pUC19, VecSource(3):PromT7, VecSource(3):PromT3, CC GenBank(50):PF1 CC #brother CC VecBase(3):BlueM13p CC #offspring CC VecBase(3):BlueKSm, VecBase(3):BlueSKm CC PARENT CC Features of BlueM13m (3204 bp) CC residue source CC 1- 238 684- 447 (c) pUC19 CC 239- 694 5488-5943 phage f1 CC 695- 858 448- 285 (c) pUC19 CC 860- 879 1- 20 T7 promoter CC 881- 937 57- 1 (c) M13mp19/pUC19-polylinker CC 943- 962 20- 1 (c) T3 promoter CC 966-1202 237- 1 (c) pUC19 CC 1203-3204 2686- 685 (c) pUC19 CC Conflict (cfl) and Mutations (mut): none CC PARENT CC Features of BlueM13m (3204 bp) CC residue source CC 1- 238 1- 238 pUC19c CC 239- 694 5488-5943 phage f1 CC 695- 853 237- 395 pUC19c CC 860- 879 1- 20 T7 promoter CC 881- 937 57- 1 (c) M13mp19/pUC19-polylinker CC 943- 962 20- 1 (c) T3 promoter CC 971-3204 453-2686 pUC19c CC Conflict (cfl) and Mutations (mut): none CC FEATURE CC 2147-2935 789-1 (c) Ap-R; b-lactamase CC POLYLINKER HindIII-SphI-PstI-SalI-XbaI-BamHI-SmaI-KpnI-SacI-EcoRI CC SELECTION CC #resistance Ap CC #indicator beta-galactosidase CC SUMMARY BlueM13m #length 3204 #checksum 257. FH Key Location/Qualifiers FT source 1..3204 FT /organism="synthetic construct" FT /mol_type="genomic DNA" FT /db_xref="taxon:32630" SQ Sequence 3204 BP; 776 A; 808 C; 802 G; 818 T; 0 other; tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgac 240 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 300 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 360 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 420 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 480 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 540 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 600 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 660 gcgaatttta acaaaatatt aacgtttaca atttcgccat tcgccattca ggctgcgcaa 720 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 780 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 840 aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc tcggtacccg 900 gggatcctct agagtcgacc tgcaggcatg caagcttttg ttccctttag tgagggttaa 960 ttccgagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 1020 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 1080 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 1140 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 1200 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 1260 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 1320 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 1380 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1440 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1500 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1560 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1620 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1680 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1740 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1800 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 1860 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1920 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1980 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 2040 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 2100 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 2160 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 2220 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 2280 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 2340 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 2400 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 2460 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2520 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2580 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2640 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2700 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2760 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 2820 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 2880 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 2940 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 3000 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 3060 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 3120 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 3180 ggcgtatcac gaggcccttt cgtc 3204 „ Bluescribe M13 - 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