Sequence of CMV-Brainbow-1.1

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Sequence of CMV-Brainbow-1.1_M

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Sequence of CMV-Brainbow-1.1 M : 8168 BP; 2083 A; 2196 C; 2097 G; 1792 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro .. CMV_fwd_pri CMV_pro .....

.......... .......... .......... .......... .......... ..........

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagctcga 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgagct 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gataacttcg tatagtatac cttatacgaa gttatcgagc tcataacttc gtataggata 660

601 ctattgaagc atatcatatg gaatatgctt caatagctcg agtattgaag catatcctat 660

.......... loxP loxP .......... .......... .......... loxP loxP

.......... .......... .......... .......... .......... ..........

661 ctttatacga agttataacg tcgagataac ttcgtatagc atacattata cgaagttata 720

661 gaaatatgct tcaatattgc agctctattg aagcatatcg tatgtaatat gcttcaatat 720

loxP loxP .......... .......... .......... loxP ..........

BglII(726) .......... .......... .......... .......... ..........

721 ccggcagatc tcgccaccat ggtgagtgtg attaaaccag agatgaagat gaagtacttc 780

721 ggccgtctag agcggtggta ccactcacac taatttggtc tctacttcta cttcatgaag 780

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

781 atggacggct ccgtcaatgg gcatgagttc acagttgaag gtgaaggcac aggcaaacct 840

781 tacctgccga ggcagttacc cgtactcaag tgtcaacttc cacttccgtg tccgtttgga 840

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tacgagggac atcaagagat gacactacgc gtcacaatgg ccaagggcgg gccaatgcct 900

841 atgctccctg tagttctcta ctgtgatgcg cagtgttacc ggttcccgcc cggttacgga 900

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttctcgtttg acttagtgtc acacacgttc tgttacggcc acagaccttt tactaaatat 960

901 aagagcaaac tgaatcacag tgtgtgcaag acaatgccgg tgtctggaaa atgatttata 960

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg 1020

961 ggtcttctct atggtctgat aaagtttgtt cgtaaaggac ttccggacag taccctttcc 1020

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcgttgcagt tcgaagatgg tgggttcgct gccgtcagtg cgcatataag ccttagagga 1080

1021 agcaacgtca agcttctacc acccaagcga cggcagtcac gcgtatattc ggaatctcct 1080

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aactgcttcg agcacaaatc caaatttgtt ggggttaact ttcctgccga tggtcctgtg 1140

1081 ttgacgaagc tcgtgtttag gtttaaacaa ccccaattga aaggacggct accaggacac 1140

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 atgcaaaacc aaagttctga ttgggagcca tcaaccgaga aaattactac ctgcgacgga 1200

1141 tacgttttgg tttcaagact aaccctcggt agttggctct tttaatgatg gacgctgcct 1200

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gttctgaagg gtgatgttac gatgtaccta aaacttgcgg gaggcggcaa tcacaaatgc 1260

1201 caagacttcc cactacaatg ctacatggat tttgaacgcc ctccgccgtt agtgtttacg 1260

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 caattcaaga ctacttacaa ggcggcaaaa aagattctta aaatgccaca aagccatttc 1320

1261 gttaagttct gatgaatgtt ccgccgtttt ttctaagaat tttacggtgt ttcggtaaag 1320

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atcgggcatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagctggt agaagatgca 1380

1321 tagcccgtag cggagcagtc cttttggctt ccgttgtaat gactcgacca tcttctacgt 1380

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtagctcatt gctgaagcgg ccgcgactct agatcataat cagccatacc acatttgtag 1440

1381 catcgagtaa cgacttcgcc ggcgctgaga tctagtatta gtcggtatgg tgtaaacatc 1440

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga 1500

1441 tccaaaatga acgaaatttt ttggagggtg tggaggggga cttggacttt gtattttact 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata 1560

1501 tacgttaaca acaacaattg aacaaataac gtcgaatatt accaatgttt atttcgttat 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca 1620

1561 cgtagtgttt aaagtgttta tttcgtaaaa aaagtgacgt aagatcaaca ccaaacaggt 1620

.......... .......... .......... .......... ......... EBV_rev_pri

.......... .......... .......... .......... .......... ..........

1621 aactcatcaa tgtatcataa cttcgtatag tataccttat acgaagttat aggatctccg 1680

1621 ttgagtagtt acatagtatt gaagcatatc atatggaata tgcttcaata tcctagaggc 1680

.......... .......... .......... loxP loxP .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccaccatgct gtgctgcatc agaagaacta aaccggttga gaagaatgaa gaggccgatc 1740

1681 ggtggtacga cacgacgtag tcttcttgat ttggccaact cttcttactt ctccggctag 1740

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aggagctgca gtcgacggta ccgcgggccc gggatccacc ggtagcatcc gccaccatgg 1800

1741 tcctcgacgt cagctgccat ggcgcccggg ccctaggtgg ccatcgtagg cggtggtacc 1800

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 1860

1801 actcgttccc gctcctccta ttgtaccggt agtagttcct caagtacgcg aagttccacg 1860

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 1920

1861 tgtacctccc gaggcacttg ccggtgctca agctctagct cccgctcccg ctcccggcgg 1920

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 1980

1921 ggatgctccc gtgggtctgg cggttcgact tccactggtt cccaccgggg gacgggaagc 1980

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 2040

1981 ggaccctgta ggacagggga gtcaagtaca tgccgaggtt ccggatgcac ttcgtggggc 2040

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 2100

2041 ggctgtaggg gctgatgaac ttcgacagga aggggctccc gaagttcacc ctcgcgcact 2100

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg 2160

2101 acttgaagct cctgccgccg caccactggc actgggtcct gaggagggac gtcctgccgc 2160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 2220

2161 tcaagtagat gttccacttc gacgcgccgt ggttgaaggg gaggctgccg gggcattacg 2220

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 2280

2221 tcttcttctg gtacccgacc ctccggagga ggctcgccta catggggctc ctgccgcggg 2280

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 2340

2281 acttcccgct ctagttcgtc tccgacttcg acttcctgcc gccggtgatg ctgcgactcc 2340

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 2400

2341 agttctggtg gatgttccgg ttcttcgggc acgtcgacgg gccgcggatg ttgcagttgt 2400

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 2460

2401 agttcaacct gtagtggagg gtgttgctcc tgatgtggta gcaccttgtc atgcttgcgc 2460

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... PmlI(2511)

2461 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaaaca cgtgcggccg 2520

2461 ggctcccggc ggtgaggtgg ccgccgtacc tgctcgacat gttcatttgt gcacgccggc 2520

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac 2580

2521 gctgagatct agtattagtc ggtatggtgt aaacatctcc aaaatgaacg aaattttttg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg 2640

2581 gagggtgtgg agggggactt ggactttgta ttttacttac gttaacaaca acaattgaac 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 2700

2641 aaataacgtc gaatattacc aatgtttatt tcgttatcgt agtgtttaaa gtgtttattt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc 2760

2701 cgtaaaaaaa gtgacgtaag atcaacacca aacaggtttg agtagttaca tagaattccg 2760

.......... .......... ......... EBV_rev_pri ......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtaaattgta agcgttaatg ctccgagggc cgccaccacc tgttcctgta ggaattggcc 2820

2761 catttaacat tcgcaattac gaggctcccg gcggtggtgg acaaggacat ccttaaccgg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... BclI(2873)

2821 gcgactctag gatctaggat aacttcgtat aggatacttt atacgaagtt attgatcaat 2880

2821 cgctgagatc ctagatccta ttgaagcata tcctatgaaa tatgcttcaa taactagtta 2880

.......... .......... .......... loxP loxP .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcacagccac catgctgtgc tgcatcagaa gaactaaacc ggttgagaag aatgaagagg 2940

2881 agtgtcggtg gtacgacacg acgtagtctt cttgatttgg ccaactcttc ttacttctcc 2940

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccgatcagga gctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg 3000

2941 ggctagtcct cgacgtcagc tgccatggcg cccgggccct aggtggccag cggtggtacc 3000

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 3060

3001 actcgttccc gctcctcgac aagtggcccc accacgggta ggaccagctc gacctgccgc 3060

.......... .......... ORF 3 EYFP .......... .......... EGFP_N_pri

.......... .......... .......... .......... .......... ..........

3061 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 3120

3061 tgcatttgcc ggtgttcaag tcgcacaggc cgctcccgct cccgctacgg tggatgccgt 3120

.......... .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 3180

3121 tcgactggga cttcaagtag acgtggtggc cgttcgacgg gcacgggacc gggtgggagc 3180

.......... .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgaccacctt cggctacggc ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc 3240

3181 actggtggaa gccgatgccg gacgtcacga agcgggcgat ggggctggtg tacttcgtcg 3240

.......... .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 3300

3241 tgctgaagaa gttcaggcgg tacgggcttc cgatgcaggt cctcgcgtgg tagaagaagt 3300

.......... .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 3360

3301 tcctgctgcc gttgatgttc tgggcgcggc tccacttcaa gctcccgctg tgggaccact 3360

.......... .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 3420

3361 tggcgtagct cgacttcccg tagctgaagt tcctcctgcc gttgtaggac cccgtgttcg 3420

.......... .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca 3480

3421 acctcatgtt gatgttgtcg gtgttgcaga tatagtaccg gctgttcgtc ttcttgccgt 3480

.......... .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 3540

3481 agttccactt gaagttctag gcggtgttgt agctcctgcc gtcgcacgtc gagcggctgg 3540

.......... .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 3600

3541 tgatggtcgt cttgtggggg tagccgctgc cggggcacga cgacgggctg ttggtgatgg 3600

.......... .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgagctacca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 3660

3601 actcgatggt caggttcgac tcgtttctgg ggttgctctt cgcgctagtg taccaggacg 3660

.......... .......... ORF 3 EYFP .......... .......... EGFP_C_pri

.......... .......... .......... .......... .......... ..........

3661 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg 3720

3661 acctcaagca ctggcggcgg ccctagtgag agccgtacct gctcgacatg ttcatttcgc 3720

EGFP_C_pri .......... ORF 3 EYFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gccgcgactc tagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 3780

3721 cggcgctgag atctagtatt agtcggtatg gtgtaaacat ctccaaaatg aacgaaattt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 3840

3781 tttggagggt gtggaggggg acttggactt tgtattttac ttacgttaac aacaacaatt 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 3900

3841 gaacaaataa cgtcgaatat taccaatgtt tatttcgtta tcgtagtgtt taaagtgttt 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 3960

3901 atttcgtaaa aaaagtgacg taagatcaac accaaacagg tttgagtagt tacatagaat 3960

.......... .......... ......... EBV_rev_pri ......... ..........

.......... .......... .......... .......... .......... ..........

3961 acgcgcgatt cgagataact tcgtatagca tacattatac gaagttatac cggaattaat 4020

3961 tgcgcgctaa gctctattga agcatatcgt atgtaatatg cttcaatatg gccttaatta 4020

.......... .......... .......... loxP .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcacagccac catgctgtgc tgcatcagaa gaactaaacc ggttgagaag aatgaagagg 4080

4021 agtgtcggtg gtacgacacg acgtagtctt cttgatttgg ccaactcttc ttacttctcc 4080

.......... .......... .......... ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccgatcagga gctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg 4140

4081 ggctagtcct cgacgtcagc tgccatggcg cccgggccct aggtggccag cggtggtacc 4140

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg 4200

4141 actcgttccc gctcctcgac aagtggcccc accacgggta ggaccagctc gacctgccgc 4200

.......... .......... ORF 3 ECFP .......... .......... EGFP_N_pri

.......... .......... .......... .......... .......... ..........

4201 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca 4260

4201 tgcatttgcc ggtgttcaag tcgcacaggc cgctcccgct cccgctacgg tggatgccgt 4260

.......... .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg 4320

4261 tcgactggga cttcaagtag acgtggtggc cgttcgacgg gcacgggacc gggtgggagc 4320

.......... .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tgaccaccct gacctggggc gtgcagtgct tcgcccgcta ccccgaccac atgaagcagc 4380

4321 actggtggga ctggaccccg cacgtcacga agcgggcgat ggggctggtg tacttcgtcg 4380

.......... .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca 4440

4381 tgctgaagaa gttcaggcgg tacgggcttc cgatgcaggt cctcgcgtgg tagaagaagt 4440

.......... .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga 4500

4441 tcctgctgcc gttgatgttc tgggcgcggc tccacttcaa gctcccgctg tgggaccact 4500

.......... .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc 4560

4501 tggcgtagct cgacttcccg tagctgaagt tcctcctgcc gttgtaggac cccgtgttcg 4560

.......... .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tggagtacaa cgccatcagc gacaacgtct atatcaccgc cgacaagcag aagaacggca 4620

4561 acctcatgtt gcggtagtcg ctgttgcaga tatagtggcg gctgttcgtc ttcttgccgt 4620

.......... .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc 4680

4621 agttccggtt gaagttctag gcggtgttgt agctcctgcc gtcgcacgtc gagcggctgg 4680

.......... .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc 4740

4681 tgatggtcgt cttgtggggg tagccgctgc cggggcacga cgacgggctg ttggtgatgg 4740

.......... .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc 4800

4741 actcgtgggt caggttcgac tcgtttctgg ggttgctctt cgcgctagtg taccaggacg 4800

.......... .......... ORF 3 ECFP .......... .......... EGFP_C_pri

.......... .......... .......... .......... .......... ..........

4801 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg 4860

4801 acctcaagca ctggcggcgg ccctagtgag agccgtacct gctcgacatg ttcatttcgc 4860

EGFP_C_pri .......... ORF 3 ECFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gccgcgactc tagatcaccg cgactctaga tcacgcgcga agttcctatt ctctagaaag 4920

4861 cggcgctgag atctagtggc gctgagatct agtgcgcgct tcaaggataa gagatctttc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tataggaact tctcgagtac tcaattgttg ttgttaactt gtttattgca gcttataatg 4980

4921 atatccttga agagctcatg agttaacaac aacaattgaa caaataacgt cgaatattac 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 5040

4981 caatgtttat ttcgttatcg tagtgtttaa agtgtttatt tcgtaaaaaa agtgacgtaa 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat 5100

5041 gatcaacacc aaacaggttt gagtagttac atagaattcc gcatttaaca ttcgcaatta 5100

......... EBV_rev_pri ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 5160

5101 taaaacaatt ttaagcgcaa tttaaaaaca atttagtcga gtaaaaaatt ggttatccgg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 5220

5161 ctttagccgt tttagggaat atttagtttt cttatctggc tctatcccaa ctcacaacaa 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 5280

5221 ggtcaaacct tgttctcagg tgataatttc ttgcacctga ggttgcagtt tcccgctttt 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 5340

5281 tggcagatag tcccgctacc gggtgatgca cttggtagtg ggattagttc aaaaaacccc 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 5400

5341 agctccacgg catttcgtga tttagccttg ggatttccct cgggggctaa atctcgaact 5400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 5460

5401 gcccctttcg gccgcttgca ccgctctttc cttcccttct ttcgctttcc tcgcccgcga 5460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 5520

5461 tcccgcgacc gttcacatcg ccagtgcgac gcgcattggt ggtgtgggcg gcgcgaatta 5520

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 5580

5521 cgcggcgatg tcccgcgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 5640

5581 acaaataaaa agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt 5640

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5641 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg 5700

5641 tacgaagtta ttataacttt ttccttctca ggactccgcc tttcttggtc gacaccttac 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 5760

5701 acacagtcaa tcccacacct ttcaggggtc cgaggggtcg tccgtcttca tacgtttcgt 5760

pBABE_3_pri ........ SV40_enh SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

5761 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 5820

5761 acgtagagtt aatcagtcgt tggtccacac ctttcagggg tccgaggggt cgtccgtctt 5820

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 5880

5821 catacgtttc gtacgtagag ttaatcagtc gttggtatca gggcggggat tgaggcgggt 5880

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 5940

5881 agggcgggga ttgaggcggg tcaaggcggg taagaggcgg ggtaccgact gattaaaaaa 5940

..... SV40_enh SV40_pro SV40_origin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 6000

5941 aataaatacg tctccggctc cggcggagcc ggagactcga taaggtcttc atcactcctc 6000

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... .......... .......... ClaI(6032) .......... ..........

6001 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt 6060

6001 cgaaaaaacc tccggatccg aaaacgtttc tagctagttc tctgtcctac tcctagcaaa 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 6120

6061 gcgtactaac ttgttctacc taacgtgcgt ccaagaggcc ggcgaaccca cctctccgat 6120

.......... .......... ....... ORF 1 NeoR/KanR ....... ..........

.......... .......... .......... .......... .......... ..........

6121 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 6180

6121 aagccgatac tgacccgtgt tgtctgttag ccgacgagac tacggcggca caaggccgac 6180

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 6240

6181 agtcgcgtcc ccgcgggcca agaaaaacag ttctggctgg acaggccacg ggacttactt 6240

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 6300

6241 gacgttctgc tccgtcgcgc cgatagcacc gaccggtgct gcccgcaagg aacgcgtcga 6300

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 6360

6301 cacgagctgc aacagtgact tcgcccttcc ctgaccgacg ataacccgct tcacggcccc 6360

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 6420

6361 gtcctagagg acagtagagt ggaacgagga cggctctttc ataggtagta ccgactacgt 6420

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 6480

6421 tacgccgccg acgtatgcga actaggccga tggacgggta agctggtggt tcgctttgta 6480

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 6540

6481 gcgtagctcg ctcgtgcatg agcctacctt cggccagaac agctagtcct actagacctg 6540

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 6600

6541 cttctcgtag tccccgagcg cggtcggctt gacaagcggt ccgagttccg ctcgtacggg 6600

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 6660

6601 ctgccgctcc tagagcagca ctgggtaccg ctacggacga acggcttata gtaccacctt 6660

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 6720

6661 ttaccggcga aaagacctaa gtagctgaca ccggccgacc cacaccgcct ggcgatagtc 6720

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 6780

6721 ctgtatcgca accgatgggc actataacga cttctcgaac cgccgcttac ccgactggcg 6780

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 6840

6781 aaggagcacg aaatgccata gcggcgaggg ctaagcgtcg cgtagcggaa gatagcggaa 6840

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 6900

6841 gaactgctca agaagactcg ccctgagacc ccaagcttta ctggctggtt cgctgcgggt 6900

ORF 1 NeoR/KanR .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 6960

6901 tggacggtag tgctctaaag ctaaggtggc ggcggaagat actttccaac ccgaagcctt 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 7020

6961 agcaaaaggc cctgcggccg acctactagg aggtcgcgcc cctagagtac gacctcaaga 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 7080

7021 agcgggtggg atccccctcc gattgacttt gtgccttcct ctgttatggc cttccttggg 7080

.......... .......... .......... TK_PA_term .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata 7140

7081 cgcgatactg ccgttatttt tctgtcttat tttgcgtgcc acaacccagc aaacaagtat 7140

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 7200

7141 ttgcgcccca agccagggtc ccgaccgtga gacagctatg gggtggctct ggggtaaccc 7200

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 7260

7201 cggttatgcg ggcgcaaaga aggaaaaggg gtggggtggg gggttcaagc ccacttccgg 7260

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata 7320

7261 gtcccgagcg tcggttgcag ccccgccgtc cgggacggta tcggagtcca atgagtatat 7320

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 7380

7321 atgaaatcta actaaatttt gaagtaaaaa ttaaattttc ctagatccac ttctaggaaa 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 7440

7381 aactattaga gtactggttt tagggaattg cactcaaaag caaggtgact cgcagtctgg 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 7500

7441 ggcatctttt ctagtttcct agaagaactc taggaaaaaa agacgcgcat tagacgacga 7500

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

7501 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 7560

7501 acgtttgttt ttttggtggc gatggtcgcc accaaacaaa cggcctagtt ctcgatggtt 7560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7561 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 7620

7561 gagaaaaagg cttccattga ccgaagtcgt ctcgcgtcta tggtttatga caggaagatc 7620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7621 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 7680

7621 acatcggcat caatccggtg gtgaagttct tgagacatcg tggcggatgt atggagcgag 7680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 7740

7681 acgattagga caatggtcac cgacgacggt caccgctatt cagcacagaa tggcccaacc 7740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7741 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 7800

7741 tgagttctgc tatcaatggc ctattccgcg tcgccagccc gacttgcccc ccaagcacgt 7800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7801 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 7860

7801 gtgtcgggtc gaacctcgct tgctggatgt ggcttgactc tatggatgtc gcactcgata 7860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7861 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 7920

7861 ctctttcgcg gtgcgaaggg cttccctctt tccgcctgtc cataggccat tcgccgtccc 7920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7921 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 7980

7921 agccttgtcc tctcgcgtgc tccctcgaag gtcccccttt gcggaccata gaaatatcag 7980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 8040

7981 gacagcccaa agcggtggag actgaactcg cagctaaaaa cactacgagc agtccccccg 8040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8041 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 8100

8041 cctcggatac ctttttgcgg tcgttgcgcc ggaaaaatgc caaggaccgg aaaacgaccg 8100

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 8160

8101 gaaaacgagt gtacaagaaa ggacgcaata ggggactaag acacctattg gcataatggc 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 ccatgcat.. .......... .......... .......... .......... .......... 8168

8161 ggtacgta.. .......... .......... .......... .......... .......... 8168

.......... .......... .......... .......... .......... ..........