GCGCID CARNEGIE1 preliminary; circular DNA; SYN; 3616 BP. XX AC M30841; ATCC37238; XX DT 15-MAR-1990 (Rel. 5, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE Drosophila/E.coli plasmid vector Carnegie 1 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RP 1-3616 RC pCIW1 from pUC8 & p6.1 RC pCIW2 from pCIW1 RC pCIW3 from pCIW2 RC pCIW4 from pCIW3 & linker RC pV9, pV10, pV11 from pCIW4 RC pV1 from pCIW4 & pUC8 RC Carnegie 1 from pCIW4 & pUC8 RC Carnegie 2 from Carnegie 1 & linker RC Carnegie 3 from Carnegie 1 & linker RC Carnegie 4 from Carnegie 1 & M13mp10 RC Carnegie 20 or car20 from Carnegie 2 & Drosophila rosy gene RA Rubin G.M., Spradling A.C.; RT "Vectors for P element-mediated gene transfer in Drosophila"; RL Nucleic Acids Res. 11:6341-6351(1983). XX CC This is a plasmid vector containing a non-autonomous P element from CC Drosophila melanogaster. It should be used with a helper plasmid such CC as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237). CC Medium is 1227 LB plus ampicillin. CC NM (Carnegie 1) CC CM (yes) CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (plasmid) CC SP () CC HO (E.coli K-12)(Drosophila melanogaster)(E.coli) CC CP () CC FN (cloning)(transposable element) CC SE () CC PA (pUC8)(Drosophila P element) CC BR () CC OF () CC OR () XX FH Key Location/Qualifiers FH FT misc_feature 0..0 FT /note="1. non-autonomous P element in white locus FT -> p6.1 FT 1. pUC8 SalI 2665bp 247..247 FT 2. p6.1 SalI-SalI, P element in white locus FT -> pCIW1 5300bp FT 1. pCIW1 BamHI FT BAL31 nuclease FT -> pCIW2 4600bp FT 1. pCIW2 SalI FT BAL31 nuclease FT -> pCIW3 3900bp FT 1. pCIW3 PvuII FT EcoRI linker 6bp gaattc FT -> pCIW4 3900bp FT 1. pCIW4 remove small EcoRI-HindIII FT 2. pUC8 EcoRI-HindIII 30bp 231..261, MCS FT -> Carnegie 1 3616bp" FT rep_origin 1..856 FT /note="ORI Drosophila P element" FT misc_recomb 856..857 FT /note="Drosophila P element DNA end/Drosophila white FT locus DNA start" FT misc_recomb 1358..1359 FT /note="Drosophila white locus DNA end/pUC8 DNA start" FT rep_origin 0..0 FT /note="ORI E. coli pMB1 (ColE1 and pBR322)" FT CDS 1763..2623 FT /note="ANT E. coli beta-lactamase gene (bla) FT ampicillin resistance gene (apr/amp)" FT misc_recomb 3388..3389 FT /note="pUC8 DNA end/Drosophila white locus DNA start" FT misc_binding 0..0 FT /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI" FT misc_binding 0..0 FT /note="SIT unique HindIII-PstI-SalI-BamHI-SmaI-EcoRI" XX SQ Sequence 3616 BP; 975 A; 857 C; 833 G; 951 T; 0 other; catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttcccgggga tccgtcgacc tgcagccaag ctttgcgtac tcgcaaatta ttaaaaataa aactttaaaa ataatttcgt ctaattaata ttatgagtta attcaaaccc cacggacatg ctaagggtta atcaacaatc atatcgctgt ctcactcaga ctcaatacga cactcagaat actattcctt tcactcgcac ttattgcaag catacgttaa gtggatgtct cttgccgacg ggaccacctt atgttatttc atcatggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgcctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggcc //  Carnegie 1 "Times New RomanGCC  pGEX_3_primer"Times New RomanGCC  AmpR_promoter"Times New RomanGCCɆ ?  Orf frame 2"Times New Roman<GC ?  Ampicillin"Times New RomanGCC  1  pBR322_origin"Times New Roman GC7EcoRI(588), SmaI(595), BamHI(598), SalI(604), PstI(614)"Times New RomanV  [eyy GCMscI"Times New Roman^ .-,%3 GCApaI"Times New Roman IIIBP GCBstBI"Times New Roman: t~ GCNdeI"Times New Roman`  2BP P GCAatII"Times New Roman_  6A_r_n GCFspI"Times New Roman  k GCtest"Times New RomanP 3Lddh GCtest"Times New Roman  ]8