Sequence of Carnegie1

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Sequence of Carnegie1

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Sequence of Carnegie 1 : 3616 BP; 975 A; 857 C; 833 G; 951 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt 60

1 gtactacttt attgtattcc accagggcag ctatcggctt cgaatggctt catatgtgaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg 120

61 tttaagtcac gtgcaaacga acaactctcc tttccaacac acgcctgctt aaaaaaaaac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag 180

121 ttttgtaatt gggaatgcac cttatttttt tttactttat aacgtttaaa acgacgtttc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg 240

181 gacactgacc tcattttaat taagtgcacg gcttcacacg ataattctct tttaacaccc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg 300

241 tcgtctcgga acccacgtcg gaaccacttt tgagggttta aacactatgg gtgaaattac 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg 360

301 taagcgtcac cttccgacgt ggacgttttc cagtctgtaa attttcctcc gctgagttgc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata 420

361 gtctacggca tggatcattt cactatctcg gacttggtct tttctatttt cttccgatat 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca 480

421 ggtcaccctc atgtgtttgt ctcattcaaa cttatcattt tttttagtaa atacatttgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa 540

481 tattgcactg acacgcaatc caggacaagt aacaaattac ttttattctc gaactccctt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... EcoRI(588) ..........

541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttcccgggga 600

541 ttttaagcat gaaacctcat gctttacgca gcaaatctcg tcgtcggctt aagggcccct 600

.......... .......... .......... .......... .......... ..........

SalI(604) PstI(614) .......... .......... .......... ..........

601 tccgtcgacc tgcagccaag ctttgcgtac tcgcaaatta ttaaaaataa aactttaaaa 660

601 aggcagctgg acgtcggttc gaaacgcatg agcgtttaat aatttttatt ttgaaatttt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ataatttcgt ctaattaata ttatgagtta attcaaaccc cacggacatg ctaagggtta 720

661 tattaaagca gattaattat aatactcaat taagtttggg gtgcctgtac gattcccaat 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 atcaacaatc atatcgctgt ctcactcaga ctcaatacga cactcagaat actattcctt 780

721 tagttgttag tatagcgaca gagtgagtct gagttatgct gtgagtctta tgataaggaa 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tcactcgcac ttattgcaag catacgttaa gtggatgtct cttgccgacg ggaccacctt 840

781 agtgagcgtg aataacgttc gtatgcaatt cacctacaga gaacggctgc cctggtggaa 840

.......... .......... .......... .......... .......... ..........

.......... .......... MscI(862) .......... .......... ..........

841 atgttatttc atcatggtct ggccattctc atcgtgagct tccgggtgct cgcatatctg 900

841 tacaataaag tagtaccaga ccggtaagag tagcactcga aggcccacga gcgtatagac 900

.......... .......... .......... .......... .......... ..........

.......... ApaI(918) .......... .......... .......... ..........

901 gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa taactgcttg 960

901 cgagattctg aagcccgggc tgcgttcctc atcggctgta tataggcttt attgacgaac 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc aaaaagctaa 1020

961 aaaaaaaaaa atggtaataa tggtagcaca aatgacaaat aacgggggag tttttcgatt 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa gtatttcccc 1080

1021 acattaatat aaacacggtt atttttgttc tatactggat atcttatgtt cataaagggg 1080

.......... .......... .......... .......... .......... ..........

BstBI(1082) ........ .......... .......... .......... ..........

1081 ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac ttacacacct 1140

1081 aagcttgtag gggtgttcat ctgaaaccta aacagaagat tggttttctg aatgtgtgga 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata tattttttat 1200

1141 cgtatggaat gtagtttttg agcaaatagc gatgtatttt gtggccctat ataaaaaata 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca ccacgtttcg 1260

1201 tatgtatgaa aagtttagcg cgcgggagaa gtattaagtg gaggtggtgt ggtgcaaagc 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt ttgttcgacg 1320

1261 atcaacgaga aagcgacaga gggtgggcga gaggcgttgt gtaagtggaa aacaagctgc 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NdeI(1376)

1321 accttggagc gactgtcgtt agttccgcgc gattcggtgc ggtatttcac accgcatatg 1380

1321 tggaacctcg ctgacagcaa tcaaggcgcg ctaagccacg ccataaagtg tggcgtatac 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 1440

1381 cacgtgagag tcatgttaga cgagactacg gcgtatcaat tcggtcgggg ctgtgggcgg 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 1500

1441 ttgtgggcga ctgcgcggga ctgcccgaac agacgagggc cgtaggcgaa tgtctgttcg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 1560

1501 acactggcag aggccctcga cgtacacagt ctccaaaagt ggcagtagtg gctttgcgcg 1560

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 1620

1561 ctctgctttc ccggagcact atgcggataa aaatatccaa ttacagtact attattacca 1620

.......... .......... .......... .......... .......... ..........

......... AatII(1631) ......... .......... .......... ..........

1621 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 1680

1621 aagaatctgc agtccaccgt gaaaagcccc tttacacgcg ccttggggat aaacaaataa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 1740

1681 aaagatttat gtaagtttat acataggcga gtactctgtt attgggacta tttacgaagt 1740

.......... ........ AmpR_promoter ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 1800

1741 tattataact ttttccttct catactcata agttgtaaag gcacagcggg aataagggaa 1800

.......... .......... .......... .... Orf frame 2 Ampicillin ....

.......... .......... .......... .......... .......... ..........

1801 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 1860

1801 aaaacgccgt aaaacggaag gacaaaaacg agtgggtctt tgcgaccact ttcattttct 1860

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 1920

1861 acgacttcta gtcaacccac gtgctcaccc aatgtagctt gacctagagt tgtcgccatt 1920

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 1980

1921 ctaggaactc tcaaaagcgg ggcttcttgc aaaaggttac tactcgtgaa aatttcaaga 1980

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 2040

1981 cgatacaccg cgccataata gggcacaact gcggcccgtt ctcgttgagc cagcggcgta 2040

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 2100

2041 tgtgataaga gtcttactga accaactcat gagtggtcag tgtcttttcg tagaatgcct 2100

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 2160

2101 accgtactgt cattctctta atacgtcacg acggtattgg tactcactat tgtgacgccg 2160

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 2220

2161 gttgaatgaa gactgttgct agcctcctgg cttcctcgat tggcgaaaaa acgtgttgta 2220

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 2280

2221 ccccctagta cattgagcgg aactagcaac ccttggcctc gacttacttc ggtatggttt 2280

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... FspI(2327) ..........

2281 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 2340

2281 gctgctcgca ctgtggtgct acggacatcg ttaccgttgt tgcaacgcgt ttgataattg 2340

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 2400

2341 accgcttgat gaatgagatc gaagggccgt tgttaattat ctgacctacc tccgcctatt 2400

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 2460

2401 tcaacgtcct ggtgaagacg cgagccggga aggccgaccg accaaataac gactatttag 2460

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 2520

2461 acctcggcca ctcgcaccca gagcgccata gtaacgtcgt gaccccggtc taccattcgg 2520

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 2580

2521 gagggcatag catcaataga tgtgctgccc ctcagtccgt tgatacctac ttgctttatc 2580

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 2640

2581 tgtctagcga ctctatccac ggagtgacta attcgtaacc attgacagtc tggttcaaat 2640

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 2700

2641 gagtatatat gaaatctaac taaattttga agtaaaaatt aaattttcct agatccactt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 2760

2701 ctaggaaaaa ctattagagt actggtttta gggaattgca ctcaaaagca aggtgactcg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 2820

2761 cagtctgggg catcttttct agtttcctag aagaactcta ggaaaaaaag acgcgcatta 2820

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2821 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 2880

2821 gacgacgaac gtttgttttt ttggtggcga tggtcgccac caaacaaacg gcctagttct 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 2940

2881 cgatggttga gaaaaaggct tccattgacc gaagtcgtct cgcgtctatg gtttatgaca 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 3000

2941 ggaagatcac atcggcatca atccggtggt gaagttcttg agacatcgtg gcggatgtat 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 3060

3001 ggagcgagac gattaggaca atggtcaccg acgacggtca ccgctattca gcacagaatg 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 3120

3061 gcccaacctg agttctgcta tcaatggcct attccgcgtc gccagcccga cttgcccccc 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 3180

3121 aagcacgtgt gtcgggtcga acctcgcttg ctggatgtgg cttgactcta tggatgtcgc 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 3240

3181 actcgtaact ctttcgcggt gcgaagggct tccctctttc cgcctgtcca taggccattc 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 3300

3241 gccgtcccag ccttgtcctc tcgcgtgctc cctcgaaggt ccccctttgc ggaccataga 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 3360

3301 aatatcagga cagcccaaag cggtggagac tgaactcgca gctaaaaaca ctacgagcag 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aggggggcgg agcctatgga aaaacgcctt cttcttgaac tcgggctcgg tgccagtata 3420

3361 tccccccgcc tcggatacct ttttgcggaa gaagaacttg agcccgagcc acggtcatat 3420

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctcaaatgg ttgtcgtacc tctcatggtt ccgttacgcc aacgagggtc tgctgattaa 3480

3421 ggagtttacc aacagcatgg agagtaccaa ggcaatgcgg ttgctcccag acgactaatt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccaatgggcg gacgtggagc cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc 3540

3481 ggttacccgc ctgcacctcg gcccgcttta atcgacgtgt agcagcttgt ggtgcacggg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cagttcgggc aaggtcatcc tggagacgct taacttctcc gccgccgatc tgccgctgga 3600

3541 gtcaagcccg ttccagtagg acctctgcga attgaagagg cggcggctag acggcgacct 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ctacgtgggt ctggcc.... .......... .......... .......... .......... 3616

3601 gatgcaccca gaccgg.... .......... .......... .......... .......... 3616

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-3616 Reference comment: pCIW1 from pUC8 & p6.1 Reference comment: pCIW2 from pCIW1 Reference comment: pCIW3 from pCIW2 Reference comment: pCIW4 from pCIW3 & linker Reference comment: pV9, pV10, pV11 from pCIW4 Reference comment: pV1 from pCIW4 & pUC8 Reference comment: Carnegie 1 from pCIW4 & pUC8 Reference comment: Carnegie 2 from Carnegie 1 & linker Reference comment: Carnegie 3 from Carnegie 1 & linker Reference comment: Carnegie 4 from Carnegie 1 & M13mp10 Reference comment: Carnegie 20 or car20 from Carnegie 2 & Drosophila rosy gene Reference author: Rubin G.M., Spradling A.C.; Reference title: "Vectors for P element-mediated gene transfer in Drosophila"; Reference location: Nucleic Acids Res. 11:6341-6351(1983).