Sequence of Carnegie2

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Sequence of Carnegie2

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Sequence of Carnegie 2 : 3622 BP; 977 A; 858 C; 834 G; 953 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt 60

1 gtactacttt attgtattcc accagggcag ctatcggctt cgaatggctt catatgtgaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg 120

61 tttaagtcac gtgcaaacga acaactctcc tttccaacac acgcctgctt aaaaaaaaac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag 180

121 ttttgtaatt gggaatgcac cttatttttt tttactttat aacgtttaaa acgacgtttc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg 240

181 gacactgacc tcattttaat taagtgcacg gcttcacacg ataattctct tttaacaccc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg 300

241 tcgtctcgga acccacgtcg gaaccacttt tgagggttta aacactatgg gtgaaattac 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg 360

301 taagcgtcac cttccgacgt ggacgttttc cagtctgtaa attttcctcc gctgagttgc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata 420

361 gtctacggca tggatcattt cactatctcg gacttggtct tttctatttt cttccgatat 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca 480

421 ggtcaccctc atgtgtttgt ctcattcaaa cttatcattt tttttagtaa atacatttgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa 540

481 tattgcactg acacgcaatc caggacaagt aacaaattac ttttattctc gaactccctt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... EcoRI(588) ..........

541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttcccgttaa 600

541 ttttaagcat gaaacctcat gctttacgca gcaaatctcg tcgtcggctt aagggcaatt 600

.......... .......... .......... .......... .......... ..........

BamHI(604) SalI(610) PstI(620) .......... .......... ..........

601 cggggatccg tcgacctgca gccaagcttt gcgtactcgc aaattattaa aaataaaact 660

601 gcccctaggc agctggacgt cggttcgaaa cgcatgagcg tttaataatt tttattttga 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttaaaaataa tttcgtctaa ttaatattat gagttaattc aaaccccacg gacatgctaa 720

661 aatttttatt aaagcagatt aattataata ctcaattaag tttggggtgc ctgtacgatt 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gggttaatca acaatcatat cgctgtctca ctcagactca atacgacact cagaatacta 780

721 cccaattagt tgttagtata gcgacagagt gagtctgagt tatgctgtga gtcttatgat 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttcctttcac tcgcacttat tgcaagcata cgttaagtgg atgtctcttg ccgacgggac 840

781 aaggaaagtg agcgtgaata acgttcgtat gcaattcacc tacagagaac ggctgccctg 840

.......... .......... .......... .......... .......... ..........

.......... .......... MscI(868) .......... .......... ..........

841 caccttatgt tatttcatca tggtctggcc attctcatcg tgagcttccg ggtgctcgca 900

841 gtggaataca ataaagtagt accagaccgg taagagtagc actcgaaggc ccacgagcgt 900

.......... .......... .......... .......... .......... ..........

.......... .......... ApaI(924) .......... .......... ..........

901 tatctggctc taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac 960

901 atagaccgag attctgaagc ccgggctgcg ttcctcatcg gctgtatata ggctttattg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgcttgtttt tttttttacc attattacca tcgtgtttac tgtttattgc cccctcaaaa 1020

961 acgaacaaaa aaaaaaatgg taataatggt agcacaaatg acaaataacg ggggagtttt 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agctaatgta attatatttg tgccaataaa aacaagatat gacctataga atacaagtat 1080

1021 tcgattacat taatataaac acggttattt ttgttctata ctggatatct tatgttcata 1080

.......... .......... .......... .......... .......... ..........

BstBI(1088) ........ .......... .......... .......... ..........

1081 ttccccttcg aacatcccca caagtagact ttggatttgt cttctaacca aaagacttac 1140

1081 aaggggaagc ttgtaggggt gttcatctga aacctaaaca gaagattggt tttctgaatg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 acacctgcat accttacatc aaaaactcgt ttatcgctac ataaaacacc gggatatatt 1200

1141 tgtggacgta tggaatgtag tttttgagca aatagcgatg tattttgtgg ccctatataa 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttttatatac atacttttca aatcgcgcgc cctcttcata attcacctcc accacaccac 1260

1201 aaaatatatg tatgaaaagt ttagcgcgcg ggagaagtat taagtggagg tggtgtggtg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gtttcgtagt tgctctttcg ctgtctccca cccgctctcc gcaacacatt caccttttgt 1320

1261 caaagcatca acgagaaagc gacagagggt gggcgagagg cgttgtgtaa gtggaaaaca 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcgacgacct tggagcgact gtcgttagtt ccgcgcgatt cggtgcggta tttcacaccg 1380

1321 agctgctgga acctcgctga cagcaatcaa ggcgcgctaa gccacgccat aaagtgtggc 1380

.......... .......... .......... .......... .......... ..........

NdeI(1382) .......... .......... .......... .......... ..........

1381 catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca 1440

1381 gtataccacg tgagagtcat gttagacgag actacggcgt atcaattcgg tcggggctgt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 1500

1441 gggcggttgt gggcgactgc gcgggactgc ccgaacagac gagggccgta ggcgaatgtc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 1560

1501 tgttcgacac tggcagaggc cctcgacgta cacagtctcc aaaagtggca gtagtggctt 1560

.......... ........ pGEX_3_primer ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 1620

1561 tgcgcgctct gctttcccgg agcactatgc ggataaaaat atccaattac agtactatta 1620

.......... .......... .......... .......... .......... ..........

......... AatII(1637) ......... .......... .......... ..........

1621 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 1680

1621 ttaccaaaga atctgcagtc caccgtgaaa agccccttta cacgcgcctt ggggataaac 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 1740

1681 aaataaaaag atttatgtaa gtttatacat aggcgagtac tctgttattg ggactattta 1740

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

1741 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 1800

1741 cgaagttatt ataacttttt ccttctcata ctcataagtt gtaaaggcac agcgggaata 1800

.......... .......... .......... .... Orf frame 2 Ampicillin ....

.......... .......... .......... .......... .......... ..........

1801 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 1860

1801 agggaaaaaa cgccgtaaaa cggaaggaca aaaacgagtg ggtctttgcg accactttca 1860

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 1920

1861 ttttctacga cttctagtca acccacgtgc tcacccaatg tagcttgacc tagagttgtc 1920

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 1980

1921 gccattctag gaactctcaa aagcggggct tcttgcaaaa ggttactact cgtgaaaatt 1980

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 2040

1981 tcaagacgat acaccgcgcc ataatagggc acaactgcgg cccgttctcg ttgagccagc 2040

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 2100

2041 ggcgtatgtg ataagagtct tactgaacca actcatgagt ggtcagtgtc ttttcgtaga 2100

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 2160

2101 atgcctaccg tactgtcatt ctcttaatac gtcacgacgg tattggtact cactattgtg 2160

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 2220

2161 acgccggttg aatgaagact gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt 2220

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 2280

2221 gttgtacccc ctagtacatt gagcggaact agcaaccctt ggcctcgact tacttcggta 2280

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... FspI(2333)

2281 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 2340

2281 tggtttgctg ctcgcactgt ggtgctacgg acatcgttac cgttgttgca acgcgtttga 2340

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 2400

2341 taattgaccg cttgatgaat gagatcgaag ggccgttgtt aattatctga cctacctccg 2400

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 2460

2401 cctatttcaa cgtcctggtg aagacgcgag ccgggaaggc cgaccgacca aataacgact 2460

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 2520

2461 atttagacct cggccactcg cacccagagc gccatagtaa cgtcgtgacc ccggtctacc 2520

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 2580

2521 attcgggagg gcatagcatc aatagatgtg ctgcccctca gtccgttgat acctacttgc 2580

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 2640

2581 tttatctgtc tagcgactct atccacggag tgactaattc gtaaccattg acagtctggt 2640

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 2700

2641 tcaaatgagt atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 2760

2701 ccacttctag gaaaaactat tagagtactg gttttaggga attgcactca aaagcaaggt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 2820

2761 gactcgcagt ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc 2820

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2821 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 2880

2821 gcattagacg acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 2940

2881 agttctcgat ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 3000

2941 atgacaggaa gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 3060

3001 atgtatggag cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 3120

3061 agaatggccc aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 3180

3121 ccccccaagc acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 3240

3181 tgtcgcactc gtaactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 3300

3241 ccattcgccg tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 3360

3301 catagaaata tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctcgtcaggg gggcggagcc tatggaaaaa cgccttcttc ttgaactcgg gctcggtgcc 3420

3361 gagcagtccc cccgcctcgg ataccttttt gcggaagaag aacttgagcc cgagccacgg 3420

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agtatacctc aaatggttgt cgtacctctc atggttccgt tacgccaacg agggtctgct 3480

3421 tcatatggag tttaccaaca gcatggagag taccaaggca atgcggttgc tcccagacga 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gattaaccaa tgggcggacg tggagccggg cgaaattagc tgcacatcgt cgaacaccac 3540

3481 ctaattggtt acccgcctgc acctcggccc gctttaatcg acgtgtagca gcttgtggtg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gtgccccagt tcgggcaagg tcatcctgga gacgcttaac ttctccgccg ccgatctgcc 3600

3541 cacggggtca agcccgttcc agtaggacct ctgcgaattg aagaggcggc ggctagacgg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctggactac gtgggtctgg cc........ .......... .......... .......... 3622

3601 cgacctgatg cacccagacc gg........ .......... .......... .......... 3622

.......... .......... .......... .......... .......... ..........