Dz?Dz?Y1 catgatgaaataacataaggtggtcccgtcgatagccgaagcttaccgaa 51 gtatacacttaaattcagtgcacgtttgcttgttgagaggaaaggttgtg 101 tgcggacgaatttttttttgaaaacattaacccttacgtggaataaaaaa 151 aaatgaaatattgcaaattttgctgcaaagctgtgactggagtaaaatta 201 attcacgtgccgaagtgtgctattaagagaaaattgtgggagcagagcct 251 tgggtgcagccttggtgaaaactcccaaatttgtgatacccactttaatg 301 attcgcagtggaaggctgcacctgcaaaaggtcagacatttaaaaggagg 351 cgactcaacgcagatgccgtacctagtaaagtgatagagcctgaaccaga 401 aaagataaaagaaggctataccagtgggagtacacaaacagagtaagttt 451 gaatagtaaaaaaaatcatttatgtaaacaataacgtgactgtgcgttag 501 gtcctgttcattgtttaatgaaaataagagcttgagggaaaaaattcgta 551 ctttggagtacgaaatgcgtcgtttagagcagcagccgaattccccggta 601 ccgggggatccgtcgacctgcagccaagctttgcgtactcgcaaattatt 651 aaaaataaaactttaaaaataatttcgtctaattaatattatgagttaat 701 tcaaaccccacggacatgctaagggttaatcaacaatcatatcgctgtct 751 cactcagactcaatacgacactcagaatactattcctttcactcgcactt 801 attgcaagcatacgttaagtggatgtctcttgccgacgggaccaccttat 851 gttatttcatcatggtctggccattctcatcgtgagcttccgggtgctcg 901 catatctggctctaagacttcgggcccgacgcaaggagtagccgacatat 951 atccgaaataactgcttgtttttttttttaccattattaccatcgtgttt 1001 actgtttattgccccctcaaaaagctaatgtaattatatttgtgccaata 1051 aaaacaagatatgacctatagaatacaagtatttccccttcgaacatccc 1101 cacaagtagactttggatttgtcttctaaccaaaagacttacacacctgc 1151 ataccttacatcaaaaactcgtttatcgctacataaaacaccgggatata 1201 ttttttatatacatacttttcaaatcgcgcgccctcttcataattcacct 1251 ccaccacaccacgtttcgtagttgctctttcgctgtctcccacccgctct 1301 ccgcaacacattcaccttttgttcgacgaccttggagcgactgtcgttag 1351 ttccgcgcgattcggtgcggtatttcacaccgcatatggtgcactctcag 1401 tacaatctgctctgatgccgcatagttaagccagccccgacacccgccaa 1451 cacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttac 1501 agacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcacc 1551 gtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttt 1601 tataggttaatgtcatgataataatggtttcttagacgtcaggtggcact 1651 tttcggggaaatgtgcgcggaacccctatttgtttatttttctaaataca 1701 ttcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaat 1751 aatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctt 1801 attcccttttttgcggcattttgccttcctgtttttgctcacccagaaac 1851 gctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggtt 1901 acatcgaactggatctcaacagcggtaagatccttgagagttttcgcccc 1951 gaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgc 2001 ggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcatac 2051 actattctcagaatgacttggttgagtactcaccagtcacagaaaagcat 2101 cttacggatggcatgacagtaagagaattatgcagtgctgccataaccat 2151 gagtgataacactgcggccaacttacttctgacaacgatcggaggaccga 2201 aggagctaaccgcttttttgcacaacatgggggatcatgtaactcgcctt 2251 gatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtga 2301 caccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactg 2351 gcgaactacttactctagcttcccggcaacaattaatagactggatggag 2401 gcggataaagttgcaggaccacttctgcgctcggcccttccggctggctg 2451 gtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatca 2501 ttgcagcactggggccagatggtaagccctcccgtatcgtagttatctac 2551 acgacggggagtcaggcaactatggatgaacgaaatagacagatcgctga 2601 gataggtgcctcactgattaagcattggtaactgtcagaccaagtttact 2651 catatatactttagattgatttaaaacttcatttttaatttaaaaggatc 2701 taggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtga 2751 gttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatctt 2801 cttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaa 2851 ccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactct 2901 ttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtcc 2951 ttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccg 3001 cctacatacctcgctctgctaatcctgttaccagtggctgctgccagtgg 3051 cgataagtcgtgtcttaccgggttggactcaagacgatagttaccggata 3101 aggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttg 3151 gagcgaacgacctacaccgaactgagatacctacagcgtgagcattgaga 3201 aagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcg 3251 gcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcc 3301 tggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcg 3351 atttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccttct 3401 tcttgaactcgggctcggtgccagtatacctcaaatggttgtcgtacctc 3451 tcatggttccgttacgccaacgagggtctgctgattaaccaatgggcgga 3501 cgtggagccgggcgaaattagctgcacatcgtcgaacaccacgtgcccca 3551 gttcgggcaaggtcatcctggagacgcttaacttctccgccgccgatctg 3601 ccgctggactacgtgggtctggcc ( Carnegie 3 "Times New RomanDz?C( pGEX_3_primer"Times New RomanDz?C( AmpR_promoter"Times New Roman<Dz?CɆ(G  Orf frame 1"Times New Roman<Dz?(G  Ampicillin"Times New RomanDz?C( 9  pBR322_origin"Times New Roman Dz?7EcoRI(588), KpnI(601), BamHI(606), SalI(612), PstI(622)"Times New Roman^( (Whzz Dz?MscI"Times New Romanf(x/-,%3 Dz?ApaI"Times New Roman(xEDD=K Dz?BstBI"Times New RomanB(lw Dz?NdeI"Times New Romanh((18 8 x Dz?AatII"Times New Romang (1DWWf Dz?FspI"Times New Roman (f Dz?test"Times New RomanX(0H``h Dz?test"Times New Roman(X3