Sequence of Carnegie3

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Sequence of Carnegie3

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Sequence of Carnegie 3 : 3624 BP; 976 A; 860 C; 836 G; 952 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt 60

1 gtactacttt attgtattcc accagggcag ctatcggctt cgaatggctt catatgtgaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg 120

61 tttaagtcac gtgcaaacga acaactctcc tttccaacac acgcctgctt aaaaaaaaac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag 180

121 ttttgtaatt gggaatgcac cttatttttt tttactttat aacgtttaaa acgacgtttc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg 240

181 gacactgacc tcattttaat taagtgcacg gcttcacacg ataattctct tttaacaccc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg 300

241 tcgtctcgga acccacgtcg gaaccacttt tgagggttta aacactatgg gtgaaattac 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg 360

301 taagcgtcac cttccgacgt ggacgttttc cagtctgtaa attttcctcc gctgagttgc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata 420

361 gtctacggca tggatcattt cactatctcg gacttggtct tttctatttt cttccgatat 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca 480

421 ggtcaccctc atgtgtttgt ctcattcaaa cttatcattt tttttagtaa atacatttgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa 540

481 tattgcactg acacgcaatc caggacaagt aacaaattac ttttattctc gaactccctt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttccccggta 600

541 ttttaagcat gaaacctcat gctttacgca gcaaatctcg tcgtcggctt aaggggccat 600

.......... .......... .......... .......... .......... ..........

KpnI(601),BamHI(606) SalI(612) PstI(622) .......... ..........

601 ccgggggatc cgtcgacctg cagccaagct ttgcgtactc gcaaattatt aaaaataaaa 660

601 ggccccctag gcagctggac gtcggttcga aacgcatgag cgtttaataa tttttatttt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ctttaaaaat aatttcgtct aattaatatt atgagttaat tcaaacccca cggacatgct 720

661 gaaattttta ttaaagcaga ttaattataa tactcaatta agtttggggt gcctgtacga 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aagggttaat caacaatcat atcgctgtct cactcagact caatacgaca ctcagaatac 780

721 ttcccaatta gttgttagta tagcgacaga gtgagtctga gttatgctgt gagtcttatg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tattcctttc actcgcactt attgcaagca tacgttaagt ggatgtctct tgccgacggg 840

781 ataaggaaag tgagcgtgaa taacgttcgt atgcaattca cctacagaga acggctgccc 840

.......... .......... .......... .......... .......... ..........

.......... .......... MscI(870) .......... .......... ..........

841 accaccttat gttatttcat catggtctgg ccattctcat cgtgagcttc cgggtgctcg 900

841 tggtggaata caataaagta gtaccagacc ggtaagagta gcactcgaag gcccacgagc 900

.......... .......... .......... .......... .......... ..........

.......... .......... ApaI(926) .......... .......... ..........

901 catatctggc tctaagactt cgggcccgac gcaaggagta gccgacatat atccgaaata 960

901 gtatagaccg agattctgaa gcccgggctg cgttcctcat cggctgtata taggctttat 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 actgcttgtt ttttttttta ccattattac catcgtgttt actgtttatt gccccctcaa 1020

961 tgacgaacaa aaaaaaaaat ggtaataatg gtagcacaaa tgacaaataa cgggggagtt 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aaagctaatg taattatatt tgtgccaata aaaacaagat atgacctata gaatacaagt 1080

1021 tttcgattac attaatataa acacggttat ttttgttcta tactggatat cttatgttca 1080

.......... .......... .......... .......... .......... ..........

BstBI(1090) ........ .......... .......... .......... ..........

1081 atttcccctt cgaacatccc cacaagtaga ctttggattt gtcttctaac caaaagactt 1140

1081 taaaggggaa gcttgtaggg gtgttcatct gaaacctaaa cagaagattg gttttctgaa 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 acacacctgc ataccttaca tcaaaaactc gtttatcgct acataaaaca ccgggatata 1200

1141 tgtgtggacg tatggaatgt agtttttgag caaatagcga tgtattttgt ggccctatat 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttttttatat acatactttt caaatcgcgc gccctcttca taattcacct ccaccacacc 1260

1201 aaaaaatata tgtatgaaaa gtttagcgcg cgggagaagt attaagtgga ggtggtgtgg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 acgtttcgta gttgctcttt cgctgtctcc cacccgctct ccgcaacaca ttcacctttt 1320

1261 tgcaaagcat caacgagaaa gcgacagagg gtgggcgaga ggcgttgtgt aagtggaaaa 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gttcgacgac cttggagcga ctgtcgttag ttccgcgcga ttcggtgcgg tatttcacac 1380

1321 caagctgctg gaacctcgct gacagcaatc aaggcgcgct aagccacgcc ataaagtgtg 1380

.......... .......... .......... .......... .......... ..........

NdeI(1384) .......... .......... .......... .......... ..........

1381 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga 1440

1381 gcgtatacca cgtgagagtc atgttagacg agactacggc gtatcaattc ggtcggggct 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac 1500

1441 gtgggcggtt gtgggcgact gcgcgggact gcccgaacag acgagggccg taggcgaatg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg 1560

1501 tctgttcgac actggcagag gccctcgacg tacacagtct ccaaaagtgg cagtagtggc 1560

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

1561 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata 1620

1561 tttgcgcgct ctgctttccc ggagcactat gcggataaaa atatccaatt acagtactat 1620

.......... .......... .......... .......... .......... ..........

......... AatII(1639) ......... .......... .......... ..........

1621 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 1680

1621 tattaccaaa gaatctgcag tccaccgtga aaagcccctt tacacgcgcc ttggggataa 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 1740

1681 acaaataaaa agatttatgt aagtttatac ataggcgagt actctgttat tgggactatt 1740

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

1741 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt 1800

1741 tacgaagtta ttataacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa 1800

.......... .......... .......... .... Orf frame 1 Ampicillin ....

.......... .......... .......... .......... .......... ..........

1801 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 1860

1801 taagggaaaa aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt 1860

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 1920

1861 cattttctac gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg 1920

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 1980

1921 tcgccattct aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa 1980

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt 2040

1981 tttcaagacg atacaccgcg ccataatagg gcacaactgc ggcccgttct cgttgagcca 2040

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 2100

2041 gcggcgtatg tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta 2100

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 2160

2101 gaatgcctac cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg 2160

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 2220

2161 tgacgccggt tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac 2220

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 2280

2221 gtgttgtacc ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg 2280

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... FspI(2335)

2281 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa 2340

2281 tatggtttgc tgctcgcact gtggtgctac ggacatcgtt accgttgttg caacgcgttt 2340

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 2400

2341 gataattgac cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc 2400

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 2460

2401 cgcctatttc aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga 2460

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 2520

2461 ctatttagac ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta 2520

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 2580

2521 ccattcggga gggcatagca tcaatagatg tgctgcccct cagtccgttg atacctactt 2580

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 2640

2581 gctttatctg tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg 2640

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 2700

2641 gttcaaatga gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 2760

2701 atccacttct aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 2820

2761 gtgactcgca gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac 2820

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2821 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 2880

2821 gcgcattaga cgacgaacgt ttgttttttt ggtggcgatg gtcgccacca aacaaacggc 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 2940

2881 ctagttctcg atggttgaga aaaaggcttc cattgaccga agtcgtctcg cgtctatggt 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 3000

2941 ttatgacagg aagatcacat cggcatcaat ccggtggtga agttcttgag acatcgtggc 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 3060

3001 ggatgtatgg agcgagacga ttaggacaat ggtcaccgac gacggtcacc gctattcagc 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 3120

3061 acagaatggc ccaacctgag ttctgctatc aatggcctat tccgcgtcgc cagcccgact 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 3180

3121 tgccccccaa gcacgtgtgt cgggtcgaac ctcgcttgct ggatgtggct tgactctatg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 3240

3181 gatgtcgcac tcgtaactct ttcgcggtgc gaagggcttc cctctttccg cctgtccata 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 3300

3241 ggccattcgc cgtcccagcc ttgtcctctc gcgtgctccc tcgaaggtcc ccctttgcgg 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 3360

3301 accatagaaa tatcaggaca gcccaaagcg gtggagactg aactcgcagc taaaaacact 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgctcgtcag gggggcggag cctatggaaa aacgccttct tcttgaactc gggctcggtg 3420

3361 acgagcagtc cccccgcctc ggataccttt ttgcggaaga agaacttgag cccgagccac 3420

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa cgagggtctg 3480

3421 ggtcatatgg agtttaccaa cagcatggag agtaccaagg caatgcggtt gctcccagac 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctgattaacc aatgggcgga cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc 3540

3481 gactaattgg ttacccgcct gcacctcggc ccgctttaat cgacgtgtag cagcttgtgg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 acgtgcccca gttcgggcaa ggtcatcctg gagacgctta acttctccgc cgccgatctg 3600

3541 tgcacggggt caagcccgtt ccagtaggac ctctgcgaat tgaagaggcg gcggctagac 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ccgctggact acgtgggtct ggcc...... .......... .......... .......... 3624

3601 ggcgacctga tgcacccaga ccgg...... .......... .......... .......... 3624

.......... .......... .......... .......... .......... ..........