Sequence of Carnegie4

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Sequence of Carnegie4

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Sequence of Carnegie 4 : 3631 BP; 978 A; 862 C; 837 G; 954 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 catgatgaaa taacataagg tggtcccgtc gatagccgaa gcttaccgaa gtatacactt 60

1 gtactacttt attgtattcc accagggcag ctatcggctt cgaatggctt catatgtgaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aaattcagtg cacgtttgct tgttgagagg aaaggttgtg tgcggacgaa tttttttttg 120

61 tttaagtcac gtgcaaacga acaactctcc tttccaacac acgcctgctt aaaaaaaaac 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aaaacattaa cccttacgtg gaataaaaaa aaatgaaata ttgcaaattt tgctgcaaag 180

121 ttttgtaatt gggaatgcac cttatttttt tttactttat aacgtttaaa acgacgtttc 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ctgtgactgg agtaaaatta attcacgtgc cgaagtgtgc tattaagaga aaattgtggg 240

181 gacactgacc tcattttaat taagtgcacg gcttcacacg ataattctct tttaacaccc 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agcagagcct tgggtgcagc cttggtgaaa actcccaaat ttgtgatacc cactttaatg 300

241 tcgtctcgga acccacgtcg gaaccacttt tgagggttta aacactatgg gtgaaattac 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 attcgcagtg gaaggctgca cctgcaaaag gtcagacatt taaaaggagg cgactcaacg 360

301 taagcgtcac cttccgacgt ggacgttttc cagtctgtaa attttcctcc gctgagttgc 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cagatgccgt acctagtaaa gtgatagagc ctgaaccaga aaagataaaa gaaggctata 420

361 gtctacggca tggatcattt cactatctcg gacttggtct tttctatttt cttccgatat 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccagtgggag tacacaaaca gagtaagttt gaatagtaaa aaaaatcatt tatgtaaaca 480

421 ggtcaccctc atgtgtttgt ctcattcaaa cttatcattt tttttagtaa atacatttgt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ataacgtgac tgtgcgttag gtcctgttca ttgtttaatg aaaataagag cttgagggaa 540

481 tattgcactg acacgcaatc caggacaagt aacaaattac ttttattctc gaactccctt 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... EcoRI(588) ..........

541 aaaattcgta ctttggagta cgaaatgcgt cgtttagagc agcagccgaa ttcgagctcg 600

541 ttttaagcat gaaacctcat gctttacgca gcaaatctcg tcgtcggctt aagctcgagc 600

.......... .......... .......... .......... .......... ..........

SmaI603),BamHI(606) XbaI(612) SalI(618) PstI(628) ..........

601 cccggggatc ctctagagtc gacctgcagc ccaagctttg cgtactcgca aattattaaa 660

601 gggcccctag gagatctcag ctggacgtcg ggttcgaaac gcatgagcgt ttaataattt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 aataaaactt taaaaataat ttcgtctaat taatattatg agttaattca aaccccacgg 720

661 ttattttgaa atttttatta aagcagatta attataatac tcaattaagt ttggggtgcc 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 acatgctaag ggttaatcaa caatcatatc gctgtctcac tcagactcaa tacgacactc 780

721 tgtacgattc ccaattagtt gttagtatag cgacagagtg agtctgagtt atgctgtgag 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agaatactat tcctttcact cgcacttatt gcaagcatac gttaagtgga tgtctcttgc 840

781 tcttatgata aggaaagtga gcgtgaataa cgttcgtatg caattcacct acagagaacg 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... MscI(877) .......... ..........

841 cgacgggacc accttatgtt atttcatcat ggtctggcca ttctcatcgt gagcttccgg 900

841 gctgccctgg tggaatacaa taaagtagta ccagaccggt aagagtagca ctcgaaggcc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ApaI(933) .......... ..........

901 gtgctcgcat atctggctct aagacttcgg gcccgacgca aggagtagcc gacatatatc 960

901 cacgagcgta tagaccgaga ttctgaagcc cgggctgcgt tcctcatcgg ctgtatatag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgaaataact gcttgttttt ttttttacca ttattaccat cgtgtttact gtttattgcc 1020

961 gctttattga cgaacaaaaa aaaaaatggt aataatggta gcacaaatga caaataacgg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ccctcaaaaa gctaatgtaa ttatatttgt gccaataaaa acaagatatg acctatagaa 1080

1021 gggagttttt cgattacatt aatataaaca cggttatttt tgttctatac tggatatctt 1080

.......... .......... .......... .......... .......... ..........

......... BstBI(1097) ......... .......... .......... ..........

1081 tacaagtatt tccccttcga acatccccac aagtagactt tggatttgtc ttctaaccaa 1140

1081 atgttcataa aggggaagct tgtaggggtg ttcatctgaa acctaaacag aagattggtt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aagacttaca cacctgcata ccttacatca aaaactcgtt tatcgctaca taaaacaccg 1200

1141 ttctgaatgt gtggacgtat ggaatgtagt ttttgagcaa atagcgatgt attttgtggc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggatatattt tttatataca tacttttcaa atcgcgcgcc ctcttcataa ttcacctcca 1260

1201 cctatataaa aaatatatgt atgaaaagtt tagcgcgcgg gagaagtatt aagtggaggt 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccacaccacg tttcgtagtt gctctttcgc tgtctcccac ccgctctccg caacacattc 1320

1261 ggtgtggtgc aaagcatcaa cgagaaagcg acagagggtg ggcgagaggc gttgtgtaag 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 accttttgtt cgacgacctt ggagcgactg tcgttagttc cgcgcgattc ggtgcggtat 1380

1321 tggaaaacaa gctgctggaa cctcgctgac agcaatcaag gcgcgctaag ccacgccata 1380

.......... .......... .......... .......... .......... ..........

.......... NdeI(1391) .......... .......... .......... ..........

1381 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 1440

1381 aagtgtggcg tataccacgt gagagtcatg ttagacgaga ctacggcgta tcaattcggt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc 1500

1441 cggggctgtg ggcggttgtg ggcgactgcg cgggactgcc cgaacagacg agggccgtag 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc 1560

1501 gcgaatgtct gttcgacact ggcagaggcc ctcgacgtac acagtctcca aaagtggcag 1560

.......... .......... ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

1561 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt 1620

1561 tagtggcttt gcgcgctctg ctttcccgga gcactatgcg gataaaaata tccaattaca 1620

.......... .......... .......... .......... .......... ..........

.......... ......... AatII(1646) ......... .......... ..........

1621 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac 1680

1621 gtactattat taccaaagaa tctgcagtcc accgtgaaaa gcccctttac acgcgccttg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 1740

1681 gggataaaca aataaaaaga tttatgtaag tttatacata ggcgagtact ctgttattgg 1740

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

1741 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 1800

1741 gactatttac gaagttatta taactttttc cttctcatac tcataagttg taaaggcaca 1800

.......... .......... .......... .... Orf frame 2 Ampicillin ....

.......... .......... .......... .......... .......... ..........

1801 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 1860

1801 gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa aaacgagtgg gtctttgcga 1860

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 1920

1861 ccactttcat tttctacgac ttctagtcaa cccacgtgct cacccaatgt agcttgacct 1920

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 1980

1921 agagttgtcg ccattctagg aactctcaaa agcggggctt cttgcaaaag gttactactc 1980

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca 2040

1981 gtgaaaattt caagacgata caccgcgcca taatagggca caactgcggc ccgttctcgt 2040

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 2100

2041 tgagccagcg gcgtatgtga taagagtctt actgaaccaa ctcatgagtg gtcagtgtct 2100

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 2160

2101 tttcgtagaa tgcctaccgt actgtcattc tcttaatacg tcacgacggt attggtactc 2160

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 2220

2161 actattgtga cgccggttga atgaagactg ttgctagcct cctggcttcc tcgattggcg 2220

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 2280

2221 aaaaaacgtg ttgtaccccc tagtacattg agcggaacta gcaacccttg gcctcgactt 2280

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 2340

2281 acttcggtat ggtttgctgc tcgcactgtg gtgctacgga catcgttacc gttgttgcaa 2340

.......... .... Orf frame 2 Ampicillin .... .......... ..........

FspI(2342) .......... .......... .......... .......... ..........

2341 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 2400

2341 cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg gccgttgtta attatctgac 2400

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 2460

2401 ctacctccgc ctatttcaac gtcctggtga agacgcgagc cgggaaggcc gaccgaccaa 2460

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 2520

2461 ataacgacta tttagacctc ggccactcgc acccagagcg ccatagtaac gtcgtgaccc 2520

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 2580

2521 cggtctacca ttcgggaggg catagcatca atagatgtgc tgcccctcag tccgttgata 2580

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 2640

2581 cctacttgct ttatctgtct agcgactcta tccacggagt gactaattcg taaccattga 2640

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 2700

2641 cagtctggtt caaatgagta tatatgaaat ctaactaaat tttgaagtaa aaattaaatt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 2760

2701 ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa ttgcactcaa 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 2820

2761 aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa ctctaggaaa 2820

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2821 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 2880

2821 aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc gccaccaaac 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 2940

2881 aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt cgtctcgcgt 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt 3000

2941 ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt tcttgagaca 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 3060

3001 tcgtggcgga tgtatggagc gagacgatta ggacaatggt caccgacgac ggtcaccgct 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 3120

3061 attcagcaca gaatggccca acctgagttc tgctatcaat ggcctattcc gcgtcgccag 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 3180

3121 cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga tgtggcttga 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga gaaaggcgga 3240

3181 ctctatggat gtcgcactcg taactctttc gcggtgcgaa gggcttccct ctttccgcct 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 3300

3241 gtccataggc cattcgccgt cccagccttg tcctctcgcg tgctccctcg aaggtccccc 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 3360

3301 tttgcggacc atagaaatat caggacagcc caaagcggtg gagactgaac tcgcagctaa 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccttcttct tgaactcggg 3420

3361 aaacactacg agcagtcccc ccgcctcgga tacctttttg cggaagaaga acttgagccc 3420

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctcggtgcca gtatacctca aatggttgtc gtacctctca tggttccgtt acgccaacga 3480

3421 gagccacggt catatggagt ttaccaacag catggagagt accaaggcaa tgcggttgct 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gggtctgctg attaaccaat gggcggacgt ggagccgggc gaaattagct gcacatcgtc 3540

3481 cccagacgac taattggtta cccgcctgca cctcggcccg ctttaatcga cgtgtagcag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gaacaccacg tgccccagtt cgggcaaggt catcctggag acgcttaact tctccgccgc 3600

3541 cttgtggtgc acggggtcaa gcccgttcca gtaggacctc tgcgaattga agaggcggcg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgatctgccg ctggactacg tgggtctggc c......... .......... .......... 3631

3601 gctagacggc gacctgatgc acccagaccg g......... .......... .......... 3631

.......... .......... .......... .......... .......... ..........