aG;!aG;!1 ttctatgctcctatattgataagaataaacttaatactataaatgaggtg 51 ttagggatttaattattctttattgatataaaaagtcctagcaatccaaa 101 tgggattgctaggaccaaacaaagtagattatatagcataaataggttta 151 attttgctacgggggcgttatttaggttttttcttctttcgaaaaaatct 201 ttctttatgaagttaaaagctatgtattcaatagcatattttgaatatgg 251 acatagaatagtgcttatcactattgcatatagcatcttatctgacacaa 301 ggaaataatacccttcgctgttttttgttataaggtatatatatataagt 351 gtgcagtacaggccaaataaaatattttttatgtagtatcttaagctcat 401 aaattaaacctcgccatatattcttttcattttataaggatcgagttatg 451 aggaaaagattttttgtgggaatattcgcgataaacctccttgttggatg 501 tcaggctaactatatacctgatgttcagggagggaccatcgcaccatcct 551 cctcttctaaactgacggggatcgcggttcagtagaaaagattaaaggat 601 cttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaa 651 aaaccaccgctaccaacggtggtttgtttgccggatcaagagctaccaac 701 tctttttccgaaggtaactggcttcagcagagcgcagataccaaatactg 751 tccttctagtgtagccgtagtcgggccactacttcaagaactctgtagca 801 ccgtttgtgccatcatcgctctgctaatccggttaccagtggctgctgcc 851 agtggcgttaaggcgtgccttaccgggttggactcaagacgatagttacc 901 ggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagccca 951 gcttggagcgaacgacctacaccgaactgagataccaacagcgtgagcta 1001 tgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggt 1051 aagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaa 1101 acgcctggtagctttatagtcctgtcgggtttcgccacctctgacttgag 1151 cgtctatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgc 1201 ctgctacgtggccttcttcctgttcctggtcttttgctcacatgttcttt 1251 ccggccttatcccctgattctgtggataactgtgttaccgtttttgtgtg 1301 agtcagtaccgctcgccgcagtcgaacgaccgagcgtagcgagtcagtga 1351 gcgaggaagcggaaaagcgcctggacgtgcattttctccttacgcatctg 1401 tgcggcatttcacacccggcatggcgtacttttcatacaatccgcactga 1451 tgccgcatggttaagccagtatacactccgctatcgctacgtgactgggt 1501 cagggctgcgccccgacacccgctaaaacctgctgacgcgccctgacggg 1551 cttgtcagctcccggcatccgctcacagacaagctgtgaccgtctccggg 1601 agctgcatgtgtcagaggttttcaccgtcatccccgaaacgtgcgaggca 1651 gctgcggtaaagctcatcggcgtggtcgtgaagcgattcacaaatatcgg 1701 cctgttcatctgcgtccagttcgttgagcttctccagcagcgttaatgtc 1751 tggcttctgataaagcgggccatgttaagggcggttttttcctgtttagt 1801 cactgatgcctccgtgtaagggggatttctgttcatggggtaatgatacc 1851 gatgaaacgcgagaggatgctcacaatacgggttactgatgatgaacatg 1901 cccggttacttgaacgctgtgagggtaaacaactggcggtatggatgcgg 1951 cgggtctgcctgggggagccggttgcccgttccggaaaactgccgacact 2001 ggcaccgccgttactgcgtcagctggccgccatcggaaataacctgaatc 2051 agacagcccgtaaggtgaacagcgggcagtggtcttccggtgaccgggtt 2101 caggtggtggccgcactgatggccatcggggatgagctgcgccggctgcg 2151 tctggctgtcagggaacagggggcgcgggatgatagttaaatttcatgcc 2201 aggggaaaaggtggtggcagtggtccggttgattacctgctggggaggga 2251 gcgtaaccgcgaaggcgcaacggtgcttcagggtataccggaagaagtcc 2301 gggaactcatcgatgccacgccatttgcgaagaaatacacgtccggtgtt 2351 ctgtcgttcgcggagaaggagctgccgccgggaggacgtgaaaaagtgat 2401 ggcgagctttgagcgtgtactgatgcccggtctcgaaaaaaatcagtaca 2451 gcatcctgtgggtggagcaccaggacaagggacggcttgagctgaatttt 2501 gtcattccgaacatggagctacagaccggaaaacgcctccagccgtacta 2551 cgaccgcgcagacaggcctagaattgatgcttggcagacgctgttaaatc 2601 accattacgggctgcatgacccgaacgccccggagaaccgcaggacgctg 2651 acactccctgataacctgcctgaaacgaaacaggcgcttgctgagggcgt 2701 cacgcgaggtatagatgcactttaccatgccggagagataaaaggccgtc 2751 aggatgtgattcaggcgctcactgaggcggggctggaagtggtcagggtg 2801 acgcgaagcagtatcagcattgcagatccgaacggcgggaagaatatcag 2851 gctgaaaggagcattttatgagcaatcttttgcagacgggcgcggagttc 2901 gagaaaaagctgaaagagagagccgaatctacagagaaaatgctgaacaa 2951 cgagttcaggaggctcggcgaatctgtaagcgaggctgtgacatcaaacg 3001 agacgaaaatcagagacgctatagccctgttcacagcctcgacagaggaa 3051 tcgctggaaaaacaccgggaaggggtgaaagaggcgatgatgcagcacag 3101 gagggacgtgttaaagctggcagggaatacgggcatgatgttactgggga 3151 tagtctttctcctgtttaccgcgagtggcgggacgctctggtatcttgga 3201 gggaggatacaggcgaacctggaagaaatcaggaagcaggaagagacatt 3251 gcagaaactgaacgcgaagacatggggcgtggagtttgtgcaggacggga 3301 acaggaaattccttgtccttccgtacgggaaatcagcggaggtgattccc 3351 tttcaggggaaagagtgggtacatctgaaggagtgacacagagtgacaga 3401 gctggaaacacatttgctgaacgccttagagcagctgcaacaggactata 3451 tgcagcggctgagcgaatgggagagcgccttcgtggaattgcagaagatg 3501 ttttcgcttacgcaacgggacaacgcgatgctgaacgagcgggtcatgca 3551 gttgagtcagcaggtgcaacacttgagcgagcagacagaacgcttgagcc 3601 agttatacagcgagaactggagataagagaggaacggctgatacaggagc 3651 gcgaacatgtgttatccctagaacgggagcgtcagccggaaatacaggaa 3701 cgcacgctggatggcccttcgctgggatggtgaaaccatgaaaaatggca 3751 gcttcagtggattaagtgggggtaatgtggcctgtaccctctggttgcat 3801 aggtattcatacggttaaaatttatcaggcgcgatcgcgcagtttttagg 3851 gtggtttgttgccatttttacctgtctgctgccgtgatcgcgctgaacgc 3901 gttttagcggtgcgtacaattaagggattatggtaaatccacttactgtc 3951 tgccctcgtagccatcgagataaaccgcagaaatcgtgtcagccagcagc 4001 ctggattttttcggggtaaggtctttgctgcggtaatcagtacccgcaaa 4051 ttgagttgtgccccggagtgagtttaaaaattcttggctgtgaaactgct 4101 gaatgcgttgttgcacatcactccgtgattttcctgtcgctttgtcttta 4151 gctcccatatcccagaactgccgaaaaaaaggcaccatcattcgtgttgt 4201 gtcggctttttgggtggaaaagttgggatcaaaaccgtcatcagaagcat 4251 atacgtaatgccgggtatgttgttccacgctgccctttggggtacaggct 4301 gtgagagctactgttgctgttatcatggcgataagacaaagtttgtttcc 4351 ggtacgtctcatgagtgtctccctaccttaaagtatttattctcggtgag 4401 ttattatccgaagactattttatcatcagtctctgacagcgttcagcgac 4451 cgaacaacctggaccagaatccccgtttcttttccagttccagtgcctgc 4501 ctgagctgggctatctcattttgtagctgttcccgttctgcttcctggcg 4551 tctgcgatccatatcctgtgcctgtttatcctcaagcatcagcgtcaggc 4601 attgtttcagctcattcagttcccggagaatgcgttctgtctgctgatca 4651 tgtggattttctgcatgtccctcactgtgcctttctggtgtctcattctg 4701 ctttaattcgccgtatgcccggatcagttcactggtttcaaactcccgtc 4751 gtccgtccctgccggtacggtaactgacaaggcctccgcgcatatcacgg 4801 taaaactgtgaacgcgatctgcctgtcatttttagtgcgtcccggagtgt 4851 gtgccatgccataaagtgacagtgtcccatagatgtctcatctcatagtt 4901 tcagtaaaacataatgaggtctgagaacggtaatgtttgtgctggttttt 4951 gtggcatcgggcgagaatagcgcgtggtgtgaaagactgtttttttgatc 5001 gttttcacaaaaatggaagtccacagtcttgacagggaaaatgcagcggc 5051 gtagcttttatgctgtatataaaaccagtggttatatgtacagtatttat 5101 ttttaacttattgttttaaaagtcaaagaggattttataatggaaaccgc 5151 ggtagcgtactataaagatggtgttccttatgatgataagggacaggtaa 5201 ttattactcttttgaatggtactcctgacgggagtggctctggcggcgga 5251 ggtggaaaaggaggcagtaaaagtgaaagttctgcagctattcatgcaac 5301 tgctaaatggtctactgctcaattaaagaaaacacaggcagagcaggctg 5351 cccgggcaaaagctgcagcggaagcacaggcgaaagcaaaggcaaacagg 5401 gatgcgctgactcagcgcctgaaggatatcgtgaatgaggctcttcgtca 5451 caatgcctcacgtacgccttcagcaacagagcttgctcatgctaataatg 5501 cagctatgcaggcggaagcagagcgtttgcgccttgcgaaagcagaagaa 5551 aaagcccgtaaagaagcggaagcagcagaaaaggcttttcaggaagcaga 5601 acaacgacgtaaagagattgaacgggagaaggctgaaacagaacgccagt 5651 tgaaactggctgaagctgaagagaaacgactggctgcattgagtgaagaa 5701 gctaaagctgttgagatcgcccaaaaaaaactttctgctgcacaatctga 5751 agtggtgaaaatggatggagagattaagactctcaattctcgtttaagct 5801 ccagtatccatgcccgtgatgcagaaatgaaaacgctcgctggaaaacga 5851 aatgaactggctcaggcatccgctaaatataaagaactggatgagctggt 5901 caaaaaactatcaccaagagccaatgatccgcttcagaaccgtccttttt 5951 ttgaagcaaccagacgacgggttggggccggtaagattagagaagaaaaa 6001 caaaaacaggtaacagcatcagaaacacgtattaaccggataaatgctga 6051 tataactcagatccagaaggctatttctcaggtcagtaataatcgtaatg 6101 ccggtatcgctcgtgttcatgaagctgaagaaaatttgaaaaaagcacag 6151 aataatctccttaattcacagattaaggatgctgttgatgcaacagttag 6201 cttttatcaaacgctgactgaaaaatatggtgaaaaatattcgaaaatgg 6251 cacaggaacttgctgataagtctaaaggtaagaaaatcggcaatgtgaat 6301 gaagctctcgctgcttttgaaaaatacaaggatgttttaaataagaaatt 6351 cagcaaagccgatcgtgatgctatttttaatgcgttggcatcggtgaagt 6401 atgatgactgggctaaacatttagatcagtttgccaagtacttgaagatt 6451 acggggcatgtttcttttggatatgatgtggtatctgatatcctaaaaat 6501 taaggatacaggtgactggaagccactatttcttacattagagaagaaag 6551 ctgcagatgcaggggtgagttatgttgttgctttactttttagcttgctt 6601 gctggaactacattaggtatttggggtattgctattgttacaggaa ColE1/Colicin E1 "Times New Roman aG;!CY pBR322_origin"Times New RomanaG;!> Orf frame 1"Times New RomanaG;!C<R pGEX_3_primer"Times New RomanaG;!CɆT Orf frame 2"Times New Roman aG;!AflII"Times New Roman VJ3$3 aG;!NruI"Times New Roman ]P4%4 aG;!ApaLI"Times New Roman {T aG;!MscI"Times New RomanJ&.99{ aG;!ClaI"Times New Roman   [ aG;!BclI"Times New Roman%maYY aG;!SacII"Times New RomancYN  N aG;!SmaI"Times New RomanmaYY aG;!EcoRI"Times New RomanIGH>G3$$j3