Sequence of ColE1

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Sequence of ColE1_ColicinE1

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Sequence of ColE1/Colicin E1 : 6646 BP; 1760 A; 1421 C; 1798 G; 1667 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttctatgctc ctatattgat aagaataaac ttaatactat aaatgaggtg ttagggattt 60

1 aagatacgag gatataacta ttcttatttg aattatgata tttactccac aatccctaaa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aattattctt tattgatata aaaagtccta gcaatccaaa tgggattgct aggaccaaac 120

61 ttaataagaa ataactatat ttttcaggat cgttaggttt accctaacga tcctggtttg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aaagtagatt atatagcata aataggttta attttgctac gggggcgtta tttaggtttt 180

121 tttcatctaa tatatcgtat ttatccaaat taaaacgatg cccccgcaat aaatccaaaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttcttctttc gaaaaaatct ttctttatga agttaaaagc tatgtattca atagcatatt 240

181 aagaagaaag cttttttaga aagaaatact tcaattttcg atacataagt tatcgtataa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttgaatatgg acatagaata gtgcttatca ctattgcata tagcatctta tctgacacaa 300

241 aacttatacc tgtatcttat cacgaatagt gataacgtat atcgtagaat agactgtgtt 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggaaataata cccttcgctg ttttttgtta taaggtatat atatataagt gtgcagtaca 360

301 cctttattat gggaagcgac aaaaaacaat attccatata tatatattca cacgtcatgt 360

.......... .......... .......... .......... .......... ..........

.......... .......... AflII(390) .......... .......... ..........

361 ggccaaataa aatatttttt atgtagtatc ttaagctcat aaattaaacc tcgccatata 420

361 ccggtttatt ttataaaaaa tacatcatag aattcgagta tttaatttgg agcggtatat 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NruI(478)

421 ttcttttcat tttataagga tcgagttatg aggaaaagat tttttgtggg aatattcgcg 480

421 aagaaaagta aaatattcct agctcaatac tccttttcta aaaaacaccc ttataagcgc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ataaacctcc ttgttggatg tcaggctaac tatatacctg atgttcaggg agggaccatc 540

481 tatttggagg aacaacctac agtccgattg atatatggac tacaagtccc tccctggtag 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gcaccatcct cctcttctaa actgacgggg atcgcggttc agtagaaaag attaaaggat 600

541 cgtggtagga ggagaagatt tgactgcccc tagcgccaag tcatcttttc taatttccta 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 660

601 gaagaactct aggaaaaaaa gacgcgcatt agacgacgaa cgtttgtttt tttggtggcg 660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 taccaacggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 720

661 atggttgcca ccaaacaaac ggcctagttc tcgatggttg agaaaaaggc ttccattgac 720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag tcgggccact 780

721 cgaagtcgtc tcgcgtctat ggtttatgac aggaagatca catcggcatc agcccggtga 780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 acttcaagaa ctctgtagca ccgtttgtgc catcatcgct ctgctaatcc ggttaccagt 840

781 tgaagttctt gagacatcgt ggcaaacacg gtagtagcga gacgattagg ccaatggtca 840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggctgctgcc agtggcgtta aggcgtgcct taccgggttg gactcaagac gatagttacc 900

841 ccgacgacgg tcaccgcaat tccgcacgga atggcccaac ctgagttctg ctatcaatgg 900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... ApaLI(937) .......... ..........

901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg 960

901 cctattccgc gtcgccagcc cgacttgccc cccaagcacg tgtgtcgggt cgaacctcgc 960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgcttcc 1020

961 ttgctggatg tggcttgact ctatggttgt cgcactcgat actctttcgc ggtgcgaagg 1020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac 1080

1021 gcttccctct ttccgcctgt ccataggcca ttcgccgtcc cagccttgtc ctctcgcgtg 1080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gagggagctt ccagggggaa acgcctggta gctttatagt cctgtcgggt ttcgccacct 1140

1081 ctccctcgaa ggtccccctt tgcggaccat cgaaatatca ggacagccca aagcggtgga 1140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctgacttgag cgtctatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc 1200

1141 gactgaactc gcagataaaa acactacgag cagtcccccc gcctcggata cctttttgcg 1200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctgctacgtg gccttcttcc tgttcctggt cttttgctca catgttcttt ccggccttat 1260

1201 gacgatgcac cggaagaagg acaaggacca gaaaacgagt gtacaagaaa ggccggaata 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cccctgattc tgtggataac tgtgttaccg tttttgtgtg agtcagtacc gctcgccgca 1320

1261 ggggactaag acacctattg acacaatggc aaaaacacac tcagtcatgg cgagcggcgt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaaaagcgc ctggacgtgc 1380

1321 cagcttgctg gctcgcatcg ctcagtcact cgctccttcg ccttttcgcg gacctgcacg 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 attttctcct tacgcatctg tgcggcattt cacacccggc atggcgtact tttcatacaa 1440

1381 taaaagagga atgcgtagac acgccgtaaa gtgtgggccg taccgcatga aaagtatgtt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tccgcactga tgccgcatgg ttaagccagt atacactccg ctatcgctac gtgactgggt 1500

1441 aggcgtgact acggcgtacc aattcggtca tatgtgaggc gatagcgatg cactgaccca 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cagggctgcg ccccgacacc cgctaaaacc tgctgacgcg ccctgacggg cttgtcagct 1560

1501 gtcccgacgc ggggctgtgg gcgattttgg acgactgcgc gggactgccc gaacagtcga 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cccggcatcc gctcacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 1620

1561 gggccgtagg cgagtgtctg ttcgacactg gcagaggccc tcgacgtaca cagtctccaa 1620

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

1621 ttcaccgtca tccccgaaac gtgcgaggca gctgcggtaa agctcatcgg cgtggtcgtg 1680

1621 aagtggcagt aggggctttg cacgctccgt cgacgccatt tcgagtagcc gcaccagcac 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aagcgattca caaatatcgg cctgttcatc tgcgtccagt tcgttgagct tctccagcag 1740

1681 ttcgctaagt gtttatagcc ggacaagtag acgcaggtca agcaactcga agaggtcgtc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttagt 1800

1741 gcaattacag accgaagact atttcgcccg gtacaattcc cgccaaaaaa ggacaaatca 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cactgatgcc tccgtgtaag ggggatttct gttcatgggg taatgatacc gatgaaacgc 1860

1801 gtgactacgg aggcacattc cccctaaaga caagtacccc attactatgg ctactttgcg 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gagaggatgc tcacaatacg ggttactgat gatgaacatg cccggttact tgaacgctgt 1920

1861 ctctcctacg agtgttatgc ccaatgacta ctacttgtac gggccaatga acttgcgaca 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gagggtaaac aactggcggt atggatgcgg cgggtctgcc tgggggagcc ggttgcccgt 1980

1921 ctcccatttg ttgaccgcca tacctacgcc gcccagacgg accccctcgg ccaacgggca 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tccggaaaac tgccgacact ggcaccgccg ttactgcgtc agctggccgc catcggaaat 2040

1981 aggccttttg acggctgtga ccgtggcggc aatgacgcag tcgaccggcg gtagccttta 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 aacctgaatc agacagcccg taaggtgaac agcgggcagt ggtcttccgg tgaccgggtt 2100

2041 ttggacttag tctgtcgggc attccacttg tcgcccgtca ccagaaggcc actggcccaa 2100

.......... .......... .......... .......... .......... ..........

.......... .......... MscI(2122) .......... .......... ..........

2101 caggtggtgg ccgcactgat ggccatcggg gatgagctgc gccggctgcg tctggctgtc 2160

2101 gtccaccacc ggcgtgacta ccggtagccc ctactcgacg cggccgacgc agaccgacag 2160

.......... .......... .......... ......... Orf frame 2 .........

.......... .......... .......... .......... .......... ..........

2161 agggaacagg gggcgcggga tgatagttaa atttcatgcc aggggaaaag gtggtggcag 2220

2161 tcccttgtcc cccgcgccct actatcaatt taaagtacgg tccccttttc caccaccgtc 2220

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tggtccggtt gattacctgc tggggaggga gcgtaaccgc gaaggcgcaa cggtgcttca 2280

2221 accaggccaa ctaatggacg acccctccct cgcattggcg cttccgcgtt gccacgaagt 2280

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... ClaI(2310) .......... .......... ..........

2281 gggtataccg gaagaagtcc gggaactcat cgatgccacg ccatttgcga agaaatacac 2340

2281 cccatatggc cttcttcagg cccttgagta gctacggtgc ggtaaacgct tctttatgtg 2340

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtccggtgtt ctgtcgttcg cggagaagga gctgccgccg ggaggacgtg aaaaagtgat 2400

2341 caggccacaa gacagcaagc gcctcttcct cgacggcggc cctcctgcac tttttcacta 2400

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggcgagcttt gagcgtgtac tgatgcccgg tctcgaaaaa aatcagtaca gcatcctgtg 2460

2401 ccgctcgaaa ctcgcacatg actacgggcc agagcttttt ttagtcatgt cgtaggacac 2460

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggtggagcac caggacaagg gacggcttga gctgaatttt gtcattccga acatggagct 2520

2461 ccacctcgtg gtcctgttcc ctgccgaact cgacttaaaa cagtaaggct tgtacctcga 2520

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 acagaccgga aaacgcctcc agccgtacta cgaccgcgca gacaggccta gaattgatgc 2580

2521 tgtctggcct tttgcggagg tcggcatgat gctggcgcgt ctgtccggat cttaactacg 2580

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttggcagacg ctgttaaatc accattacgg gctgcatgac ccgaacgccc cggagaaccg 2640

2581 aaccgtctgc gacaatttag tggtaatgcc cgacgtactg ggcttgcggg gcctcttggc 2640

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 caggacgctg acactccctg ataacctgcc tgaaacgaaa caggcgcttg ctgagggcgt 2700

2641 gtcctgcgac tgtgagggac tattggacgg actttgcttt gtccgcgaac gactcccgca 2700

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cacgcgaggt atagatgcac tttaccatgc cggagagata aaaggccgtc aggatgtgat 2760

2701 gtgcgctcca tatctacgtg aaatggtacg gcctctctat tttccggcag tcctacacta 2760

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tcaggcgctc actgaggcgg ggctggaagt ggtcagggtg acgcgaagca gtatcagcat 2820

2761 agtccgcgag tgactccgcc ccgaccttca ccagtcccac tgcgcttcgt catagtcgta 2820

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgcagatccg aacggcggga agaatatcag gctgaaagga gcattttatg agcaatcttt 2880

2821 acgtctaggc ttgccgccct tcttatagtc cgactttcct cgtaaaatac tcgttagaaa 2880

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgcagacggg cgcggagttc gagaaaaagc tgaaagagag agccgaatct acagagaaaa 2940

2881 acgtctgccc gcgcctcaag ctctttttcg actttctctc tcggcttaga tgtctctttt 2940

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgctgaacaa cgagttcagg aggctcggcg aatctgtaag cgaggctgtg acatcaaacg 3000

2941 acgacttgtt gctcaagtcc tccgagccgc ttagacattc gctccgacac tgtagtttgc 3000

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agacgaaaat cagagacgct atagccctgt tcacagcctc gacagaggaa tcgctggaaa 3060

3001 tctgctttta gtctctgcga tatcgggaca agtgtcggag ctgtctcctt agcgaccttt 3060

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aacaccggga aggggtgaaa gaggcgatga tgcagcacag gagggacgtg ttaaagctgg 3120

3061 ttgtggccct tccccacttt ctccgctact acgtcgtgtc ctccctgcac aatttcgacc 3120

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cagggaatac gggcatgatg ttactgggga tagtctttct cctgtttacc gcgagtggcg 3180

3121 gtcccttatg cccgtactac aatgacccct atcagaaaga ggacaaatgg cgctcaccgc 3180

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggacgctctg gtatcttgga gggaggatac aggcgaacct ggaagaaatc aggaagcagg 3240

3181 cctgcgagac catagaacct ccctcctatg tccgcttgga ccttctttag tccttcgtcc 3240

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aagagacatt gcagaaactg aacgcgaaga catggggcgt ggagtttgtg caggacggga 3300

3241 ttctctgtaa cgtctttgac ttgcgcttct gtaccccgca cctcaaacac gtcctgccct 3300

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 acaggaaatt ccttgtcctt ccgtacggga aatcagcgga ggtgattccc tttcagggga 3360

3301 tgtcctttaa ggaacaggaa ggcatgccct ttagtcgcct ccactaaggg aaagtcccct 3360

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aagagtgggt acatctgaag gagtgacaca gagtgacaga gctggaaaca catttgctga 3420

3361 ttctcaccca tgtagacttc ctcactgtgt ctcactgtct cgacctttgt gtaaacgact 3420

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acgccttaga gcagctgcaa caggactata tgcagcggct gagcgaatgg gagagcgcct 3480

3421 tgcggaatct cgtcgacgtt gtcctgatat acgtcgccga ctcgcttacc ctctcgcgga 3480

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tcgtggaatt gcagaagatg ttttcgctta cgcaacggga caacgcgatg ctgaacgagc 3540

3481 agcaccttaa cgtcttctac aaaagcgaat gcgttgccct gttgcgctac gacttgctcg 3540

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gggtcatgca gttgagtcag caggtgcaac acttgagcga gcagacagaa cgcttgagcc 3600

3541 cccagtacgt caactcagtc gtccacgttg tgaactcgct cgtctgtctt gcgaactcgg 3600

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agttatacag cgagaactgg agataagaga ggaacggctg atacaggagc gcgaacatgt 3660

3601 tcaatatgtc gctcttgacc tctattctct ccttgccgac tatgtcctcg cgcttgtaca 3660

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gttatcccta gaacgggagc gtcagccgga aatacaggaa cgcacgctgg atggcccttc 3720

3661 caatagggat cttgccctcg cagtcggcct ttatgtcctt gcgtgcgacc taccgggaag 3720

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg attaagtggg ggtaatgtgg 3780

3721 cgaccctacc actttggtac tttttaccgt cgaagtcacc taattcaccc ccattacacc 3780

Orf frame 2 ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cctgtaccct ctggttgcat aggtattcat acggttaaaa tttatcaggc gcgatcgcgc 3840

3781 ggacatggga gaccaacgta tccataagta tgccaatttt aaatagtccg cgctagcgcg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agtttttagg gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc 3900

3841 tcaaaaatcc caccaaacaa cggtaaaaat ggacagacga cggcactagc gcgacttgcg 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gttttagcgg tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta 3960

3901 caaaatcgcc acgcatgtta attccctaat accatttagg tgaatgacag acgggagcat 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gccatcgaga taaaccgcag aaatcgtgtc agccagcagc ctggattttt tcggggtaag 4020

3961 cggtagctct atttggcgtc tttagcacag tcggtcgtcg gacctaaaaa agccccattc 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gtctttgctg cggtaatcag tacccgcaaa ttgagttgtg ccccggagtg agtttaaaaa 4080

4021 cagaaacgac gccattagtc atgggcgttt aactcaacac ggggcctcac tcaaattttt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttcttggctg tgaaactgct gaatgcgttg ttgcacatca ctccgtgatt ttcctgtcgc 4140

4081 aagaaccgac actttgacga cttacgcaac aacgtgtagt gaggcactaa aaggacagcg 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tttgtcttta gctcccatat cccagaactg ccgaaaaaaa ggcaccatca ttcgtgttgt 4200

4141 aaacagaaat cgagggtata gggtcttgac ggcttttttt ccgtggtagt aagcacaaca 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtcggctttt tgggtggaaa agttgggatc aaaaccgtca tcagaagcat atacgtaatg 4260

4201 cagccgaaaa acccaccttt tcaaccctag ttttggcagt agtcttcgta tatgcattac 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ccgggtatgt tgttccacgc tgccctttgg ggtacaggct gtgagagcta ctgttgctgt 4320

4261 ggcccataca acaaggtgcg acgggaaacc ccatgtccga cactctcgat gacaacgaca 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tatcatggcg ataagacaaa gtttgtttcc ggtacgtctc atgagtgtct ccctacctta 4380

4321 atagtaccgc tattctgttt caaacaaagg ccatgcagag tactcacaga gggatggaat 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aagtatttat tctcggtgag ttattatccg aagactattt tatcatcagt ctctgacagc 4440

4381 ttcataaata agagccactc aataataggc ttctgataaa atagtagtca gagactgtcg 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gttcagcgac cgaacaacct ggaccagaat ccccgtttct tttccagttc cagtgcctgc 4500

4441 caagtcgctg gcttgttgga cctggtctta ggggcaaaga aaaggtcaag gtcacggacg 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctgagctggg ctatctcatt ttgtagctgt tcccgttctg cttcctggcg tctgcgatcc 4560

4501 gactcgaccc gatagagtaa aacatcgaca agggcaagac gaaggaccgc agacgctagg 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 atatcctgtg cctgtttatc ctcaagcatc agcgtcaggc attgtttcag ctcattcagt 4620

4561 tataggacac ggacaaatag gagttcgtag tcgcagtccg taacaaagtc gagtaagtca 4620

.......... .......... .......... .......... .......... ..........

.......... .......... BclI(4645) .......... .......... ..........

4621 tcccggagaa tgcgttctgt ctgctgatca tgtggatttt ctgcatgtcc ctcactgtgc 4680

4621 agggcctctt acgcaagaca gacgactagt acacctaaaa gacgtacagg gagtgacacg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctttctggtg tctcattctg ctttaattcg ccgtatgccc ggatcagttc actggtttca 4740

4681 gaaagaccac agagtaagac gaaattaagc ggcatacggg cctagtcaag tgaccaaagt 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aactcccgtc gtccgtccct gccggtacgg taactgacaa ggcctccgcg catatcacgg 4800

4741 ttgagggcag caggcaggga cggccatgcc attgactgtt ccggaggcgc gtatagtgcc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 taaaactgtg aacgcgatct gcctgtcatt tttagtgcgt cccggagtgt gtgccatgcc 4860

4801 attttgacac ttgcgctaga cggacagtaa aaatcacgca gggcctcaca cacggtacgg 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ataaagtgac agtgtcccat agatgtctca tctcatagtt tcagtaaaac ataatgaggt 4920

4861 tatttcactg tcacagggta tctacagagt agagtatcaa agtcattttg tattactcca 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ctgagaacgg taatgtttgt gctggttttt gtggcatcgg gcgagaatag cgcgtggtgt 4980

4921 gactcttgcc attacaaaca cgaccaaaaa caccgtagcc cgctcttatc gcgcaccaca 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gaaagactgt ttttttgatc gttttcacaa aaatggaagt ccacagtctt gacagggaaa 5040

4981 ctttctgaca aaaaaactag caaaagtgtt tttaccttca ggtgtcagaa ctgtcccttt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 atgcagcggc gtagctttta tgctgtatat aaaaccagtg gttatatgta cagtatttat 5100

5041 tacgtcgccg catcgaaaat acgacatata ttttggtcac caatatacat gtcataaata 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... SacII(5150) .........

5101 ttttaactta ttgttttaaa agtcaaagag gattttataa tggaaaccgc ggtagcgtac 5160

5101 aaaattgaat aacaaaattt tcagtttctc ctaaaatatt acctttggcg ccatcgcatg 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tataaagatg gtgttcctta tgatgataag ggacaggtaa ttattactct tttgaatggt 5220

5161 atatttctac cacaaggaat actactattc cctgtccatt aataatgaga aaacttacca 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 actcctgacg ggagtggctc tggcggcgga ggtggaaaag gaggcagtaa aagtgaaagt 5280

5221 tgaggactgc cctcaccgag accgccgcct ccaccttttc ctccgtcatt ttcactttca 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tctgcagcta ttcatgcaac tgctaaatgg tctactgctc aattaaagaa aacacaggca 5340

5281 agacgtcgat aagtacgttg acgatttacc agatgacgag ttaatttctt ttgtgtccgt 5340

.......... .......... .......... .......... .......... ..........

.......... SmaI(5353) .......... .......... .......... ..........

5341 gagcaggctg cccgggcaaa agctgcagcg gaagcacagg cgaaagcaaa ggcaaacagg 5400

5341 ctcgtccgac gggcccgttt tcgacgtcgc cttcgtgtcc gctttcgttt ccgtttgtcc 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gatgcgctga ctcagcgcct gaaggatatc gtgaatgagg ctcttcgtca caatgcctca 5460

5401 ctacgcgact gagtcgcgga cttcctatag cacttactcc gagaagcagt gttacggagt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgtacgcctt cagcaacaga gcttgctcat gctaataatg cagctatgca ggcggaagca 5520

5461 gcatgcggaa gtcgttgtct cgaacgagta cgattattac gtcgatacgt ccgccttcgt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gagcgtttgc gccttgcgaa agcagaagaa aaagcccgta aagaagcgga agcagcagaa 5580

5521 ctcgcaaacg cggaacgctt tcgtcttctt tttcgggcat ttcttcgcct tcgtcgtctt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aaggcttttc aggaagcaga acaacgacgt aaagagattg aacgggagaa ggctgaaaca 5640

5581 ttccgaaaag tccttcgtct tgttgctgca tttctctaac ttgccctctt ccgactttgt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gaacgccagt tgaaactggc tgaagctgaa gagaaacgac tggctgcatt gagtgaagaa 5700

5641 cttgcggtca actttgaccg acttcgactt ctctttgctg accgacgtaa ctcacttctt 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gctaaagctg ttgagatcgc ccaaaaaaaa ctttctgctg cacaatctga agtggtgaaa 5760

5701 cgatttcgac aactctagcg ggtttttttt gaaagacgac gtgttagact tcaccacttt 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 atggatggag agattaagac tctcaattct cgtttaagct ccagtatcca tgcccgtgat 5820

5761 tacctacctc tctaattctg agagttaaga gcaaattcga ggtcataggt acgggcacta 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gcagaaatga aaacgctcgc tggaaaacga aatgaactgg ctcaggcatc cgctaaatat 5880

5821 cgtctttact tttgcgagcg accttttgct ttacttgacc gagtccgtag gcgatttata 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aaagaactgg atgagctggt caaaaaacta tcaccaagag ccaatgatcc gcttcagaac 5940

5881 tttcttgacc tactcgacca gttttttgat agtggttctc ggttactagg cgaagtcttg 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cgtccttttt ttgaagcaac cagacgacgg gttggggccg gtaagattag agaagaaaaa 6000

5941 gcaggaaaaa aacttcgttg gtctgctgcc caaccccggc cattctaatc tcttcttttt 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 caaaaacagg taacagcatc agaaacacgt attaaccgga taaatgctga tataactcag 6060

6001 gtttttgtcc attgtcgtag tctttgtgca taattggcct atttacgact atattgagtc 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 atccagaagg ctatttctca ggtcagtaat aatcgtaatg ccggtatcgc tcgtgttcat 6120

6061 taggtcttcc gataaagagt ccagtcatta ttagcattac ggccatagcg agcacaagta 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gaagctgaag aaaatttgaa aaaagcacag aataatctcc ttaattcaca gattaaggat 6180

6121 cttcgacttc ttttaaactt ttttcgtgtc ttattagagg aattaagtgt ctaattccta 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gctgttgatg caacagttag cttttatcaa acgctgactg aaaaatatgg tgaaaaatat 6240

6181 cgacaactac gttgtcaatc gaaaatagtt tgcgactgac tttttatacc actttttata 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tcgaaaatgg cacaggaact tgctgataag tctaaaggta agaaaatcgg caatgtgaat 6300

6241 agcttttacc gtgtccttga acgactattc agatttccat tcttttagcc gttacactta 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gaagctctcg ctgcttttga aaaatacaag gatgttttaa ataagaaatt cagcaaagcc 6360

6301 cttcgagagc gacgaaaact ttttatgttc ctacaaaatt tattctttaa gtcgtttcgg 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gatcgtgatg ctatttttaa tgcgttggca tcggtgaagt atgatgactg ggctaaacat 6420

6361 ctagcactac gataaaaatt acgcaaccgt agccacttca tactactgac ccgatttgta 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ttagatcagt ttgccaagta cttgaagatt acggggcatg tttcttttgg atatgatgtg 6480

6421 aatctagtca aacggttcat gaacttctaa tgccccgtac aaagaaaacc tatactacac 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gtatctgata tcctaaaaat taaggataca ggtgactgga agccactatt tcttacatta 6540

6481 catagactat aggattttta attcctatgt ccactgacct tcggtgataa agaatgtaat 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gagaagaaag ctgcagatgc aggggtgagt tatgttgttg ctttactttt tagcttgctt 6600

6541 ctcttctttc gacgtctacg tccccactca atacaacaac gaaatgaaaa atcgaacgaa 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRI(6644) .........

6601 gctggaacta cattaggtat ttggggtatt gctattgtta caggaa.... .......... 6646

6601 cgaccttgat gtaatccata aaccccataa cgataacaat gtcctt.... .......... 6646

.......... .......... .......... .......... .......... ..........