Cre_Lox Plasmids
CMV-Brainbow-1.1_M to pBAC-BA-lacZ | pBAC-lacZ to pCAG-ERT2CreERT2 | pEZ-Frt-lox-DT to pRho-Cre | V308 pDNR MCS lacZ alpha to V956 pLPS-3'ECFP - Acceptor |
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| Plasmids 0 - 4 | Descriptions |
 | CMV-Brainbow-1.1 M - Gene/insert name: Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean; GenBank/Entrez ID of insert: AB128820; Species of gene(s): Other; Vector backbone: pEGFP-N1 (Search Vector Database); Backbone manufacturer: Clontech; 5' Sequencing primer: CMV-F (List of Sequencing Primers); Bacteria resistance: Kanamycin; High or low copy: Don't Know; Grow in standard E. coli @ 3... |
 | Lox-Stop-Lox TOPO - Gene/insert name: loxP-STOP-loxP; Species of gene(s): SV40; Vector backbone: pCR-Blunt II-TOPO (Search Vector Database); Backbone manufacturer: Invitrogen; 5' Sequencing primer: M13R (List of Sequencing Primers); Bacteria resistance: Kanamycin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Bacterial strain for growth and growth condition: However... |
 | LV-Cre-SD - Gene/insert name: nlsCre; Alternative names: nls Cre LV-Cre cre; Gene/insert aliases: cre; Species of gene(s): Enterobacteriophage P1; Relevant mutations/deletions: loxP site introduced downstream of nlsCre; Vector backbone: n/a (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': KpnI; Site destroyed during cloning: No; 5'... |
 | p079 pPNT6 - Gene/insert name: na; Vector backbone: pPNT6 (Search Vector Database); 5' Sequencing primer: M13R (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Selectable markers: Neomycin; Sequence: View sequence; Plasmid Provided In: DH5a; Principal Investigator: Ramesh Shivdasani; Comments: Targeting vector... |
 | pBAC-BA-lacZ - Gene/insert name: BA; Alternative names: Binding site for original BCR-ABL three-finger array; Vector backbone: pBAC-lacZ (Search Vector Database); Cloning site 5': BsaI; Site destroyed during cloning: No; Cloning site 3': BsaI; Site destroyed during cloning: No; 5' Sequencing primer: CGC CAG GGT TTT CCC AGT CAC GAC (List of Sequencing Primers); Bacteria resistance: Chlo... |
| Plasmids 5 - 9 | Descriptions |
 | pBAC-lacZ - Gene/insert name: none; Vector backbone: pBAC-lacZ (Search Vector Database); Cloning site 5': BsaI; Site destroyed during cloning: No; Cloning site 3': BsaI; Site destroyed during cloning: No; 5' Sequencing primer: CGC CAG GGT TTT CCC AGT CAC GAC (List of Sequencing Primers); Bacteria resistance: Chloramphenicol; High or low copy: High Copy; Grow in standard E. coli @ 37C: Y... |
 | pBOB-CAG-iCRE-SD - Gene/insert name: iCRE; Alternative names: improved CRE; Species of gene(s): Other; Relevant mutations/deletions: improved CRE expression by applying mammalian codon usage to CRE recombinase.; Vector backbone: pBOB (Search Vector Database); Cloning site 5': XbaI; Site destroyed during cloning: No; Cloning site 3': XhoI; Site destroyed during cloning: No; 5' Sequen... |
 | pCAG-Cre - Gene/insert name: Cre; Alternative names: Cre recombinase; Species of gene(s): Bacteriophage P1; Fusion proteins or tags: Myc; Terminal: C terminal on insert; Vector backbone: pCAGEN (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' Sequencing primer: pCAG-F (List of Sequencing Pr... |
 | pCAG-CreERT2 - Gene/insert name: Cre-ERT2 fusion protein; Alternative names: inducible Cre; Species of gene(s): Bacteriophage P1; Vector backbone: pCAGEN (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' Sequencing primer: pCAG-F (List of Sequencing Primers); Bacteria resistance: Ampicilli... |
 | pCAG-ERT2CreERT2 - Gene/insert name: ERT2-Cre-ERT2 fusion protein; Alternative names: inducible Cre; Species of gene(s): Bacteriophage P1; Vector backbone: pCAGEN (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' Sequencing primer: pCAG-F (List of Sequencing Primers); Bacteria resistance... |
| Plasmids 10 - 14 | Descriptions |
 | pEZ-Frt-lox-DT - Gene/insert name: pEZ-Frt-lox-DT; Species of gene(s): DNA derived from yeast; E. Coli; transposon Tn5; Cosynebacterium; Vector backbone: pBSII KS (Search Vector Database); Backbone manufacturer: Stratagene; Cloning site 5': Cla I; Site destroyed during cloning: No; Cloning site 3': Don't Know; Site destroyed during cloning: Yes; 5' Sequencing primer: T7/M13 (List of ... |
 | pLB - Fusion proteins or tags: GFP ;
Terminal: C terminal on backbone ;
Vector backbone: pLL3.7 ;
Backbone manufacturer: N/A ;
Cloning site 5': HpaI ;
Site destroyed during cloning: No ;
Cloning site 3': XhoI ;
Site destroyed during cloning: No ;
5' Sequencing primer: mU6-F ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. co... |
 | pNrl-Cre - Gene/insert name: Nrl promoter-Cre; Alternative names: Nrl; Gene/insert aliases: Nrl, AW492574, D14H14S46E; Species of gene(s): M. musculus (mouse); Fusion proteins or tags: Cre; Terminal: C terminal on backbone; Vector backbone: na (Search Vector Database); Cloning site 5': SalI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' ... |
 | pPGK-Cre-bpA - Gene/insert name: Phosphoglycerate kinase 1 promoter; Alternative names: PGK; Species of gene(s): bacteriophage P1; Vector backbone: XhopKS (Search Vector Database); Backbone manufacturer: Stratagene; Cloning site 5': Sall; Site destroyed during cloning: No; Cloning site 3': Not I; Site destroyed during cloning: No; 5' Sequencing primer: n/a (List of Sequencing Primers... |
 | pRho-Cre - Gene/insert name: Rhodopsin promoter-Cre; Alternative names: Cre recombinase; Fusion proteins or tags: Myc; Terminal: C terminal on insert; Vector backbone: pCAGEN? (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' Sequencing primer: na (List of Sequencing Primers); Bacteria resista... |
| Plasmids 15 - 19 | Descriptions |
 | V308 pDNR MCS lacZ alpha - Relevant mutations/deletions: AscI, PacI, and LacZ added to MCS ;
Vector backbone: pDNR Dual;
Backbone manufacturer: Clontech;
5' Sequencing primer: M13R ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes;
Plasmid Provided In: DH5a
Principal Investigator: Tony Pawson
; Comments: Donor vector for th... |
 | Relevant mutations/deletions: Altered MCS with AscI and PacI -
Vector backbone: pDNR Dual ;
Backbone manufacturer: Clontech;
5' Sequencing primer: M13 forward ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes;
Plasmid Provided In: DH5a ;
Principal Investigator: Tony Pawson ;
Comments: Donor vector for the Creator Spl... |
 | Relevant mutations/deletions: Splice for 3' Tags -
Fusion proteins or tags: ECFP ;
Terminal: C terminal on backbone ;
Vector backbone: NA ;
5' Sequencing primer: CMV-F ;
Bacteria resistance: Kanamycin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes ;
Plasmid Provided In: DH5a ;
Principal Investigator: Tony Pawson ;
Comments: Acceptor vector ... |
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