BV Tech Plasmid
Plasmid drawing software

Cre_Lox Plasmids

  • V308 pDNR MCS lacZ alpha
    Relevant mutations/deletions: AscI, PacI, and LacZ added to MCS
    Vector backbone: pDNR Dual
    Backbone manufacturer: Clontech
    Type of vector: Cre/Lox,Donor vector
    Backbone size (bp): 5138
    5' Sequencing primer: M13R
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Plasmid Provided In: DH5a Principal Investigator: Tony Pawson
    Comments: Donor vector for the Creator Splice system.
    Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)
  • V7 pDNR Dual - Donor
    Relevant mutations/deletions: Altered MCS with AscI and PacI
    Vector backbone: pDNR Dual
    Backbone manufacturer: Clontech
    Type of vector: Cre/Lox,Donor vector
    Backbone size (bp): 4827
    5' Sequencing primer: M13 forward
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Plasmid Provided In: DH5a
    Principal Investigator: Tony Pawson
    Comments: Donor vector for the Creator Splice system.
    Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)
  • V956 pLPS-3'ECFP - Acceptor
    Relevant mutations/deletions: Splice for 3' Tags
    Fusion proteins or tags: ECFP
    Terminal: C terminal on backbone
    Vector backbone: NA
    Type of vector: Mammalian expression,Cre/Lox,Acceptor vector
    Backbone size (bp): 4879
    5' Sequencing primer: CMV-F
    Bacteria resistance: Kanamycin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Plasmid Provided In: DH5a
    Principal Investigator: Tony Pawson
    Comments: Acceptor vector for the Creator Splice system.
    Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)
  • pLB
    Fusion proteins or tags: GFP
    Terminal: C terminal on backbone
    Vector backbone: pLL3.7
    Backbone manufacturer: N/A
    Type of vector: Mammalian expression,Lentiviral,RNAi,Cre/Lox
    Backbone size (bp): 8500
    Cloning site 5': HpaI
    Site destroyed during cloning: No
    Cloning site 3': XhoI
    Site destroyed during cloning: No
    5' Sequencing primer: mU6-F
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Plasmid Provided In: DH5a
    Principal Investigator: Stephan Kissler
    Comments: pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP. Note: A single base pair deletion at position 11 of the U6 promoter in this plasmid does not impair the efficacy of this reagent.
    Article: In vivo RNA interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler S et al. (Nat Genet. 2006 Apr . 38(4):479-83. Pubmed)



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