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Cre_Lox Plasmids


CMV-Brainbow-1.1_M to pBAC-BA-lacZ | pBAC-lacZ to pCAG-ERT2CreERT2 | pEZ-Frt-lox-DT to pRho-Cre | V308 pDNR MCS lacZ alpha to V956 pLPS-3'ECFP - Acceptor | 

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Plasmids 0 - 4Descriptions
small image of CMV-Brainbow-1.1_MCMV-Brainbow-1.1 M - Gene/insert name: Kusabira ; M-mCherry ; M-mEYFP ; M-mCerulean; GenBank/Entrez ID of insert: AB128820; Species of gene(s): Other; Vector backbone: pEGFP-N1 (Search Vector Database); Backbone manufacturer: Clontech; 5' Sequencing primer: CMV-F (List of Sequencing Primers); Bacteria resistance: Kanamycin; High or low copy: Don't Know; Grow in standard E. coli @ 3...
small image of Lox-Stop-LoxLox-Stop-Lox TOPO - Gene/insert name: loxP-STOP-loxP; Species of gene(s): SV40; Vector backbone: pCR-Blunt II-TOPO (Search Vector Database); Backbone manufacturer: Invitrogen; 5' Sequencing primer: M13R (List of Sequencing Primers); Bacteria resistance: Kanamycin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Bacterial strain for growth and growth condition: However...
small image of LV-Cre-SDLV-Cre-SD - Gene/insert name: nlsCre; Alternative names: nls Cre LV-Cre cre; Gene/insert aliases: cre; Species of gene(s): Enterobacteriophage P1; Relevant mutations/deletions: loxP site introduced downstream of nlsCre; Vector backbone: n/a (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': KpnI; Site destroyed during cloning: No; 5'...
small image of p079_pPNT6p079 pPNT6 - Gene/insert name: na; Vector backbone: pPNT6 (Search Vector Database); 5' Sequencing primer: M13R (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Selectable markers: Neomycin; Sequence: View sequence; Plasmid Provided In: DH5a; Principal Investigator: Ramesh Shivdasani; Comments: Targeting vector...
small image of pBAC-BA-lacZpBAC-BA-lacZ - Gene/insert name: BA; Alternative names: Binding site for original BCR-ABL three-finger array; Vector backbone: pBAC-lacZ (Search Vector Database); Cloning site 5': BsaI; Site destroyed during cloning: No; Cloning site 3': BsaI; Site destroyed during cloning: No; 5' Sequencing primer: CGC CAG GGT TTT CCC AGT CAC GAC (List of Sequencing Primers); Bacteria resistance: Chlo...

Plasmids 5 - 9Descriptions
small image of pBAC-lacZpBAC-lacZ - Gene/insert name: none; Vector backbone: pBAC-lacZ (Search Vector Database); Cloning site 5': BsaI; Site destroyed during cloning: No; Cloning site 3': BsaI; Site destroyed during cloning: No; 5' Sequencing primer: CGC CAG GGT TTT CCC AGT CAC GAC (List of Sequencing Primers); Bacteria resistance: Chloramphenicol; High or low copy: High Copy; Grow in standard E. coli @ 37C: Y...
small image of pBOB-CAG-iCRE-SDpBOB-CAG-iCRE-SD - Gene/insert name: iCRE; Alternative names: improved CRE; Species of gene(s): Other; Relevant mutations/deletions: improved CRE expression by applying mammalian codon usage to CRE recombinase.; Vector backbone: pBOB (Search Vector Database); Cloning site 5': XbaI; Site destroyed during cloning: No; Cloning site 3': XhoI; Site destroyed during cloning: No; 5' Sequen...
small image of pCAG-CrepCAG-Cre - Gene/insert name: Cre; Alternative names: Cre recombinase; Species of gene(s): Bacteriophage P1; Fusion proteins or tags: Myc; Terminal: C terminal on insert; Vector backbone: pCAGEN (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' Sequencing primer: pCAG-F (List of Sequencing Pr...
small image of pCAG-CreERT2pCAG-CreERT2 - Gene/insert name: Cre-ERT2 fusion protein; Alternative names: inducible Cre; Species of gene(s): Bacteriophage P1; Vector backbone: pCAGEN (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' Sequencing primer: pCAG-F (List of Sequencing Primers); Bacteria resistance: Ampicilli...
small image of pCAG-ERT2CreERT2pCAG-ERT2CreERT2 - Gene/insert name: ERT2-Cre-ERT2 fusion protein; Alternative names: inducible Cre; Species of gene(s): Bacteriophage P1; Vector backbone: pCAGEN (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' Sequencing primer: pCAG-F (List of Sequencing Primers); Bacteria resistance...

Plasmids 10 - 14Descriptions
small image of pEZ-Frt-lox-DTpEZ-Frt-lox-DT - Gene/insert name: pEZ-Frt-lox-DT; Species of gene(s): DNA derived from yeast; E. Coli; transposon Tn5; Cosynebacterium; Vector backbone: pBSII KS (Search Vector Database); Backbone manufacturer: Stratagene; Cloning site 5': Cla I; Site destroyed during cloning: No; Cloning site 3': Don't Know; Site destroyed during cloning: Yes; 5' Sequencing primer: T7/M13 (List of ...
small image of pLBpLB - Fusion proteins or tags: GFP ; Terminal: C terminal on backbone ; Vector backbone: pLL3.7 ; Backbone manufacturer: N/A ; Cloning site 5': HpaI ; Site destroyed during cloning: No ; Cloning site 3': XhoI ; Site destroyed during cloning: No ; 5' Sequencing primer: mU6-F ; Bacteria resistance: Ampicillin ; High or low copy: High Copy ; Grow in standard E. co...
small image of pNrl-CrepNrl-Cre - Gene/insert name: Nrl promoter-Cre; Alternative names: Nrl; Gene/insert aliases: Nrl, AW492574, D14H14S46E; Species of gene(s): M. musculus (mouse); Fusion proteins or tags: Cre; Terminal: C terminal on backbone; Vector backbone: na (Search Vector Database); Cloning site 5': SalI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' ...
small image of pPGK-Cre-bpApPGK-Cre-bpA - Gene/insert name: Phosphoglycerate kinase 1 promoter; Alternative names: PGK; Species of gene(s): bacteriophage P1; Vector backbone: XhopKS (Search Vector Database); Backbone manufacturer: Stratagene; Cloning site 5': Sall; Site destroyed during cloning: No; Cloning site 3': Not I; Site destroyed during cloning: No; 5' Sequencing primer: n/a (List of Sequencing Primers...
small image of pRho-CrepRho-Cre - Gene/insert name: Rhodopsin promoter-Cre; Alternative names: Cre recombinase; Fusion proteins or tags: Myc; Terminal: C terminal on insert; Vector backbone: pCAGEN? (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site 3': NotI; Site destroyed during cloning: No; 5' Sequencing primer: na (List of Sequencing Primers); Bacteria resista...

Plasmids 15 - 19Descriptions
small image of V308 pDNR MCS lacZ alphaV308 pDNR MCS lacZ alpha - Relevant mutations/deletions: AscI, PacI, and LacZ added to MCS ; Vector backbone: pDNR Dual; Backbone manufacturer: Clontech; 5' Sequencing primer: M13R ; Bacteria resistance: Ampicillin ; High or low copy: High Copy ; Grow in standard E. coli @ 37C: Yes; Plasmid Provided In: DH5a Principal Investigator: Tony Pawson ; Comments: Donor vector for th...
small image of V7 pDNR Dual - DonorRelevant mutations/deletions: Altered MCS with AscI and PacI - Vector backbone: pDNR Dual ; Backbone manufacturer: Clontech; 5' Sequencing primer: M13 forward ; Bacteria resistance: Ampicillin ; High or low copy: High Copy ; Grow in standard E. coli @ 37C: Yes; Plasmid Provided In: DH5a ; Principal Investigator: Tony Pawson ; Comments: Donor vector for the Creator Spl...
small image of V956 pLPS-3'ECFP - AcceptorRelevant mutations/deletions: Splice for 3' Tags - Fusion proteins or tags: ECFP ; Terminal: C terminal on backbone ; Vector backbone: NA ; 5' Sequencing primer: CMV-F ; Bacteria resistance: Kanamycin ; High or low copy: High Copy ; Grow in standard E. coli @ 37C: Yes ; Plasmid Provided In: DH5a ; Principal Investigator: Tony Pawson ; Comments: Acceptor vector ...



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