|
V308 pDNR MCS lacZ alpha
Relevant mutations/deletions: AscI, PacI, and LacZ added to MCS
Vector backbone: pDNR Dual
Backbone manufacturer: Clontech
Type of vector: Cre/Lox,Donor vector
Backbone size (bp): 5138
5' Sequencing primer: M13R
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Tony Pawson
Comments: Donor vector for the Creator Splice system.
Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)
V7 pDNR Dual - Donor
Relevant mutations/deletions: Altered MCS with AscI and PacI
Vector backbone: pDNR Dual
Backbone manufacturer: Clontech
Type of vector: Cre/Lox,Donor vector
Backbone size (bp): 4827
5' Sequencing primer: M13 forward
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Tony Pawson
Comments: Donor vector for the Creator Splice system.
Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)
V956 pLPS-3'ECFP - Acceptor
Relevant mutations/deletions: Splice for 3' Tags
Fusion proteins or tags: ECFP
Terminal: C terminal on backbone
Vector backbone: NA
Type of vector: Mammalian expression,Cre/Lox,Acceptor vector
Backbone size (bp): 4879
5' Sequencing primer: CMV-F
Bacteria resistance: Kanamycin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Tony Pawson
Comments: Acceptor vector for the Creator Splice system.
Article: Modification of the Creator recombination system for proteomics applications--improved expression by addition of splice sites. Colwill K et al. (BMC Biotechnol. 2006 . 6():13. Pubmed)
pLB
Fusion proteins or tags: GFP
Terminal: C terminal on backbone
Vector backbone: pLL3.7
Backbone manufacturer: N/A
Type of vector: Mammalian expression,Lentiviral,RNAi,Cre/Lox
Backbone size (bp): 8500
Cloning site 5': HpaI
Site destroyed during cloning: No
Cloning site 3': XhoI
Site destroyed during cloning: No
5' Sequencing primer: mU6-F
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Stephan Kissler
Comments: pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP.
Note: A single base pair deletion at position 11 of the U6 promoter in this plasmid does not impair the efficacy of this reagent.
Article: In vivo RNA interference demonstrates a role for Nramp1 in modifying susceptibility to type 1 diabetes. Kissler S et al. (Nat Genet. 2006 Apr . 38(4):479-83. Pubmed)
|