Sequence of EFCMVRFP

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of EFCMVRFP

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of EF.CMV.RFP : 8220 BP; 2046 A; 2001 C; 2195 G; 1978 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac 60

1 gtccaccgtg aaaagcccct ttacacgcgc cttggggata aacaaataaa aagatttatg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa 120

61 taagtttata cataggcgag tactctgtta ttgggactat ttacgaagtt attataactt 120

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 180

121 tttccttctc atactcataa gttgtaaagg cacagcggga ataagggaaa aaacgccgta 180

.......... .......... ....... ORF3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 240

181 aaacggaagg acaaaaacga gtgggtcttt gcgaccactt tcattttcta cgacttctag 240

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 300

241 tcaacccacg tgctcaccca atgtagcttg acctagagtt gtcgccattc taggaactct 300

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 360

301 caaaagcggg gcttcttgca aaaggttact actcgtgaaa atttcaagac gatacaccgc 360

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 420

361 gccataatag ggcataactg cggcccgttc tcgttgagcc agcggcgtat gtgataagag 420

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 480

421 tcttactgaa ccaactcatg agtggtcagt gtcttttcgt agaatgccta ccgtactgtc 480

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 540

481 attctcttaa tacgtcacga cggtattggt actcactatt gtgacgccgg ttgaatgaag 540

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 600

541 actgttgcta gcctcctggc ttcctcgatt ggcgaaaaaa cgtgttgtac cccctagtac 600

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 660

601 attgagcgga actagcaacc cttggcctcg acttacttcg gtatggtttg ctgctcgcac 660

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 720

661 tgtggtgcta cggacatcgt taccgttgtt gcaacgcgtt tgataattga ccgcttgatg 720

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 780

721 aatgagatcg aagggccgtt gttaattatc tgacctacct ccgcctattt caacgtcctg 780

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 840

781 gtgaagacgc gagccgggaa ggccgaccga ccaaataacg actatttaga cctcggccac 840

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 900

841 tcgcacccag agcgccatag taacgtcgtg accccggtct accattcggg agggcatagc 900

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 960

901 atcaatagat gtgctgcccc tcagtccgtt gatacctact tgctttatct gtctagcgac 960

.......... ....... ORF3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 1020

961 tctatccacg gagtgactaa ttcgtaacca ttgacagtct ggttcaaatg agtatatatg 1020

....... ORF3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 1080

1021 aaatctaact aaattttgaa gtaaaaatta aattttccta gatccacttc taggaaaaac 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg 1140

1081 tattagagta ctggttttag ggaattgcac tcaaaagcaa ggtgactcgc agtctggggc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc 1200

1141 atcttttcta gtttcctaga agaactctag gaaaaaaaga cgcgcattag acgacgaacg 1200

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc 1260

1201 tttgtttttt tggtggcgat ggtcgccacc aaacaaacgg cctagttctc gatggttgag 1260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt 1320

1261 aaaaaggctt ccattgaccg aagtcgtctc gcgtctatgg tttatgacag gaagatcaca 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc 1380

1321 tcggcatcaa tccggtggtg aagttcttga gacatcgtgg cggatgtatg gagcgagacg 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact 1440

1381 attaggacaa tggtcaccga cgacggtcac cgctattcag cacagaatgg cccaacctga 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac 1500

1441 gttctgctat caatggccta ttccgcgtcg ccagcccgac ttgcccccca agcacgtgtg 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag 1560

1501 tcgggtcgaa cctcgcttgc tggatgtggc ttgactctat ggatgtcgca ctcgatactc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg 1620

1561 tttcgcggtg cgaagggctt ccctctttcc gcctgtccat aggccattcg ccgtcccagc 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 1680

1621 cttgtcctct cgcgtgctcc ctcgaaggtc cccctttgcg gaccatagaa atatcaggac 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 1740

1681 agcccaaagc ggtggagact gaactcgcag ctaaaaacac tacgagcagt ccccccgcct 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 1800

1741 cggatacctt tttgcggtcg ttgcgccgga aaaatgccaa ggaccggaaa acgaccggaa 1800

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct 1860

1801 aacgagtgta caagaaagga cgcaataggg gactaagaca cctattggca taatggcgga 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg 1920

1861 aactcactcg actatggcga gcggcgtcgg cttgctggct cgcgtcgctc agtcactcgc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1980

1921 tccttcgcct tctcgcgggt tatgcgtttg gcggagaggg gcgcgcaacc ggctaagtaa 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 2040

1981 ttacgtcgac cgtgctgtcc aaagggctga cctttcgccc gtcactcgcg ttgcgttaat 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta 2100

2041 tacactcaat cgagtgagta atccgtgggg tccgaaatgt gaaatacgaa ggccgagcat 2100

.......... .......... .......... .......... lac_pro ..........

.......... .......... .......... .......... .......... ..........

2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt 2160

2101 acaacacacc ttaacactcg cctattgtta aagtgtgtcc tttgtcgata ctggtactaa 2160

.......... .......... M13_pUC_r .......... M13_r ..........

.......... .......... .......... .......... .......... ..........

2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat 2220

2161 tgcggttcgc gcgttaattg ggagtgattt cccttgtttt cgacctcgac gttcgaatta 2220

.......... .......... T3_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc 2280

2221 catcagaata cgttatgaga acatcagaac gttgtaccat tgctactcaa tcgttgtacg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg 2340

2281 gaatgttcct ctctttttcg tggcacgtac ggctaaccac cttcattcca ccatgctagc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgga 2400

2341 acggaataat ccttccgttg tctgcccaga ctgtacctaa cctgcttggt gacttaacct 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg 2460

2401 ccgcaccgga cccgccctga cccctcaccg ctcgggagtc taggacgtat attcgtcgac 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 2520

2461 gaaaaacgga catgacccag agagaccaat ctggtctaga ctcggaccct cgagagaccg 2520

.......... .......... ......... HIV truncHIV ......... ..........

.......... .......... .......... .......... .......... ..........

2521 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 2580

2521 attgatccct tgggtgacga attcggagtt atttcgaacg gaactcacga agttcatcac 2580

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 2640

2581 acacgggcag acaacacact gagaccattg atctctaggg agtctgggaa aatcagtcac 2640

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc 2700

2641 accttttaga gatcgtcacc gcgggcttgt ccctggactt tcgctttccc tttggtctcg 2700

HIV truncHIV ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac 2760

2701 agagagctgc gtcctgagcc gaacgacttc gcgcgtgccg ttctccgctc cccgccgctg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga 2820

2761 accactcatg cggtttttaa aactgatcgc ctccgatctt cctctctcta cccacgctct 2820

.......... ........ HIV-1_psi_pack ........ .......... ..........

.......... .......... .......... NruI(2852) .......... ..........

2821 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag 2880

2821 cgcagtcata attcgccccc tcttaatcta gcgctaccct tttttaagcc aattccggtc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat 2940

2881 cccctttctt ttttatattt aattttgtat atcatacccg ttcgtccctc gatcttgcta 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc 3000

2941 agcgtcaatt aggaccggac aatctttgta gtcttccgac atctgtttat gaccctgtcg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa 3060

3001 atgttggtag ggaagtctgt cctagtcttc ttgaatctag taatatatta tgtcatcgtt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga 3120

3061 gggagataac acacgtagtt tcctatctct attttctgtg gttccttcga aatctgttct 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga 3180

3121 atctccttct cgttttgttt tcattctggt ggcgtgtcgt tcgccggcga ctagaagtct 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta 3240

3181 ggacctcctc ctctatactc cctgttaacc tcttcactta atatatttat atttcatcat 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa 3300

3241 ttttaacttg gtaatcctca tcgtgggtgg ttccgtttct cttctcacca cgtctctctt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact 3360

3301 ttttctcgtc acccttatcc tcgaaacaag gaacccaaga accctcgtcg tccttcgtga 3360

.......... .......... .......... RRE .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atgggcgcag cctcaatgac gctgacggta caggccagac aattattgtc tggtatagtg 3420

3361 tacccgcgtc ggagttactg cgactgccat gtccggtctg ttaataacag accatatcac 3420

.......... .......... RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca 3480

3421 gtcgtcgtct tgttaaacga ctcccgataa ctccgcgttg tcgtagacaa cgttgagtgt 3480

.......... .......... RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat 3540

3481 cagaccccgt agttcgtcga ggtccgttct taggaccgac acctttctat ggatttccta 3540

.......... .......... RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct 3600

3541 gttgtcgagg acccctaaac cccaacgaga ccttttgagt aaacgtggtg acgacacgga 3600

RRE .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tggaatgcta gttggagtaa taaatctctg gaacagattg gaatcacacg acctggatgg 3660

3601 accttacgat caacctcatt atttagagac cttgtctaac cttagtgtgc tggacctacc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc 3720

3661 tcaccctgtc tctttaattg ttaatgtgtt cgaattatgt gaggaattaa cttcttagcg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt 3780

3721 ttttggtcgt tcttttctta cttgttctta ataaccttaa tctatttacc cgttcaaaca 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag 3840

3781 ccttaaccaa attgtattgt ttaaccgaca ccatatattt taataagtat tactatcatc 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc 3900

3841 ctccgaacca tccaaattct tatcaaaaac gacatgaaag atatcactta tctcaatccg 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc 3960

3901 tccctataag tggtaatagc aaagtctggg tggagggttg gggctcccct gggctgtccg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga 4020

3961 ggcttcctta tcttcttctt ccacctctct ctctgtctct gtctaggtaa gctaatcact 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 acggatctcg acggtatcga tcacgagact agcctcgaga agcttgatga cggtatcgat 4080

4021 tgcctagagc tgccatagct agtgctctga tcggagctct tcgaactact gccatagcta 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct 4140

4081 ttcgaaacgt ttctacctat ttcaaaattt gtctctcctt agaaacgtcg attacctgga 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tctaggtctt gaaaggagtg cctcgtgagg ctccggtgcc cgtcagtggg cagagcgcac 4200

4141 agatccagaa ctttcctcac ggagcactcc gaggccacgg gcagtcaccc gtctcgcgtg 4200

.......... .......... .......... .......... EF1a_pro ..........

.......... .......... .......... .......... .......... ..........

4201 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag 4260

4201 tagcgggtgt caggggctct tcaacccccc tccccagccg ttaacttggc cacggatctc 4260

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga 4320

4261 ttccaccgcg ccccatttga ccctttcact acagcacatg accgaggcgg aaaaagggct 4320

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg 4380

4321 cccaccccct cttggcatat attcacgtca tcagcggcac ttgcaagaaa aagcgttgcc 4380

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac 4440

4381 caaacggcgg tcttgtgtcc attcacggca cacaccaagg gcgcccggac cggagaaatg 4440

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga 4500

4441 cccaataccg ggaacgcacg gaacttaatg aaggtggacc gacgtcatgc actaagaact 4500

.......... .......... EF1a_pro .......... ORF3 ..........

.......... .......... .......... .......... .......... ..........

4501 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc 4560

4501 agggctcgaa gcccaacctt cacccaccct ctcaagctcc ggaacgcgaa ttcctcgggg 4560

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg 4620

4561 aagcggagca cgaactcaac tccggaccgg acccgcgacc ccggcggcgc acgcttagac 4620

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg 4680

4621 caccgtggaa gcgcggacag agcgacgaaa gctattcaga gatcggtaaa ttttaaaaac 4680

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct 4740

4681 tactggacga cgctgcgaaa aaaagaccgt tctatcagaa catttacgcc cggttctaga 4740

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg 4800

4741 cgtgtgacca taaagccaaa aaccccggcg cccgccgctg ccccgggcac gcagggtcgc 4800

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc 4860

4801 gtgtacaagc cgctccgccc cggacgctcg cgccggtggc tcttagcctg cccccatcag 4860

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg 4920

4861 agttcgaccg gccggacgag accacggacc ggagcgcggc ggcacatagc ggggcgggac 4920

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg 4980

4921 ccgccgttcc gaccgggcca gccgtggtca acgcactcgc ctttctaccg gcgaagggcc 4980

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc 5040

4981 gggacgacgt ccctcgagtt ttacctcctg cgccgcgagc cctctcgccc gcccactcag 5040

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga 5100

5041 tgggtgtgtt tccttttccc ggaaaggcag gagtcggcag cgaagtacac tgaggtgcct 5100

.......... ........ EF1a_pro ORF3 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt 5160

5101 catggcccgc ggcaggtccg tggagctaat caagagctcg aaaacctcat gcagcagaaa 5160

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga 5220

5161 tccaaccccc ctccccaaaa tacgctacct caaaggggtg tgactcaccc acctctgact 5220

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg 5280

5221 tcaatccggt cgaaccgtga actacattaa gaggaacctt aaacgggaaa aactcaaacc 5280

.......... .......... EF1a_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt 5340

5281 tagaaccaag taagagttcg gagtctgtca ccaagtttca aaaaaagaag gtaaagtcca 5340

.......... ....... EF1a_pro EF1a_f ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gtcgtgagga attagcttgg tactaatacg actcactata gggagaccca agctggctag 5400

5341 cagcactcct taatcgaacc atgattatgc tgagtgatat ccctctgggt tcgaccgatc 5400

.......... .......... .......... T7_pro .......... ..........

......... EcoRV(5411) ......... .......... .......... ..........

5401 ttaagcttga tatcgaattc ctgcagccct aatagtaatc aattacgggg tcattagttc 5460

5401 aattcgaact atagcttaag gacgtcggga ttatcattag ttaatgcccc agtaatcaag 5460

.......... .......... .......... ......... CMV_imm_pro .........

.......... .......... .......... .......... .......... ..........

5461 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 5520

5461 tatcgggtat atacctcaag gcgcaatgta ttgaatgcca tttaccgggc ggaccgactg 5520

.......... ......... CMV_imm_pro ......... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

5521 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 5580

5521 gcgggttgct gggggcgggt aactgcagtt attactgcat acaagggtat cattgcggtt 5580

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... NdeI(5639)

5581 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 5640

5581 atccctgaaa ggtaactgca gttacccacc tcataaatgc catttgacgg gtgaaccgtc 5640

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc 5700

5641 atgtagttca catagtatac ggttcatgcg ggggataact gcagttactg ccatttaccg 5700

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct 5760

5701 ggcggaccgt aatacgggtc atgtactgga ataccctgaa aggatgaacc gtcatgtaga 5760

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg 5820

5761 tgcataatca gtagcgataa tggtaccact acgccaaaac cgtcatgtag ttacccgcac 5820

......... CAG_enh CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt 5880

5821 ctatcgccaa actgagtgcc cctaaaggtt cagaggtggg gtaactgcag ttaccctcaa 5880

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga 5940

5881 acaaaaccgt ggttttagtt gccctgaaag gttttacagc attgttgagg cggggtaact 5940

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga 6000

5941 gcgtttaccc gccatccgca catgccaccc tccagatata ttcgtctcga ccaaatcact 6000

......... CMV_f_pri CMV_imm_pro CMV_pro ......... ..........

.......... NheI(6013) .......... ........ BstBI(6049) BamHI(6059)

6001 accgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattggggg 6060

6001 tggcagtcta ggcgatcgcg atggcctgag tctagagctc gagttcgaag cttaaccccc 6060

CMV_pro .......... .......... .......... .......... ORF3

.......... .......... .......... .......... .......... ..........

6061 atccaccggt cgccaccatg gcctcctccg agaacgtcat caccgagttc atgcgcttca 6120

6061 taggtggcca gcggtggtac cggaggaggc tcttgcagta gtggctcaag tacgcgaagt 6120

.......... ......... ORF3 ORF3 DsRed2 ......... ..........

.......... .......... .......... .......... .......... ..........

6121 aggtgcgcat ggagggcacc gtgaacggcc acgagttcga gatcgagggc gagggcgagg 6180

6121 tccacgcgta cctcccgtgg cacttgccgg tgctcaagct ctagctcccg ctcccgctcc 6180

.......... ....... ORF3 ORF3 DsRed2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gccgccccta cgagggccac aacaccgtga agctgaaggt gaccaagggc ggccccctgc 6240

6181 cggcggggat gctcccggtg ttgtggcact tcgacttcca ctggttcccg ccgggggacg 6240

.......... ....... ORF3 ORF3 DsRed2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ccttcgcctg ggacatcctg tccccccagt tccagtacgg ctccaaggtg tacgtgaagc 6300

6241 ggaagcggac cctgtaggac aggggggtca aggtcatgcc gaggttccac atgcacttcg 6300

.......... ORF3 ORF3 DsRed2 dsRed1_N_pri .......... ..........

.......... .......... .......... .......... .......... ..........

6301 accccgccga catccccgac tacaagaagc tgtccttccc cgagggcttc aagtgggagc 6360

6301 tggggcggct gtaggggctg atgttcttcg acaggaaggg gctcccgaag ttcaccctcg 6360

.......... ....... ORF3 ORF3 DsRed2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gcgtgatgaa cttcgaggac ggcggcgtgg cgaccgtgac ccaggactcc tccctgcagg 6420

6361 cgcactactt gaagctcctg ccgccgcacc gctggcactg ggtcctgagg agggacgtcc 6420

.......... ....... ORF3 ORF3 DsRed2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 acggctgctt catctacaag gtgaagttca tcggcgtgaa cttcccctcc gacggccccg 6480

6421 tgccgacgaa gtagatgttc cacttcaagt agccgcactt gaaggggagg ctgccggggc 6480

.......... ....... ORF3 ORF3 DsRed2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tgatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg 6540

6481 actacgtctt cttctggtac ccgaccctcc ggaggtggct cgcggacatg ggggcgctgc 6540

.......... ....... ORF3 ORF3 DsRed2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gcgtgctgaa gggcgagacc cacaaggccc tgaagctgaa ggacggcggc cactacctgg 6600

6541 cgcacgactt cccgctctgg gtgttccggg acttcgactt cctgccgccg gtgatggacc 6600

.......... ....... ORF3 ORF3 DsRed2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tggagttcaa gtccatctac atggccaaga agcccgtgca gctgcccggc tactactacg 6660

6601 acctcaagtt caggtagatg taccggttct tcgggcacgt cgacgggccg atgatgatgc 6660

.......... ....... ORF3 ORF3 DsRed2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 tggacgccaa gctggacatc acctcccaca acgaggacta caccatcgtg gagcagtacg 6720

6661 acctgcggtt cgacctgtag tggagggtgt tgctcctgat gtggtagcac ctcgtcatgc 6720

.......... ORF3 ORF3 DsRed2 dsRed1_C_pri .......... ..........

.......... .......... .......... .......... .......... ..........

6721 agcgcaccga gggccgccac cacctgttcc tgtagcggcc gcgactctag atcataatca 6780

6721 tcgcgtggct cccggcggtg gtggacaagg acatcgccgg cgctgagatc tagtattagt 6780

......... ORF3 DsRed2 ORF3 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 6840

6781 cggtatggtg taaacatctc caaaatgaac gaaatttttt ggagggtgtg gagggggact 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... HpaI(6875) .......... ..........

6841 acctgaaaca taaaatgaat gcaattgttg ttgttaacgg gcccgcggaa ttccgtttaa 6900

6841 tggactttgt attttactta cgttaacaac aacaattgcc cgggcgcctt aaggcaaatt 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6960

6901 ctggttactg aatgttccgt cgacatctag aatcggtgaa aaattttctt ttcccccctg 6960

.......... .......... .......... .......... .......... cPPT U3PPT

.......... .......... .......... .......... .......... ..........

6961 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc 7020

6961 accttcccga ttaagtgagg gttgcttctg ttctagacga aaaacgaaca tgacccagag 7020

.......... cPPT U3PPT delta_U3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 7080

7021 agaccaatct ggtctagact cggaccctcg agagaccgat tgatcccttg ggtgacgaat 7080

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 7140

7081 tcggagttat ttcgaacgga actcacgaag ttcatcacac acgggcagac aacacactga 7140

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 7200

7141 gaccattgat ctctagggag tctgggaaaa tcagtcacac cttttagaga tcgtcatcat 7200

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 7260

7201 caagtacagt agaataataa gtcataaata ttgaacgttt ctttacttat agtctctcac 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 7320

7261 tctccttgaa caaataacgt cgaatattac caatgtttat ttcgttatcg tagtgtttaa 7320

.......... .......... ......... SV40_PA_term ......... ..........

.......... .......... .......... .......... .......... ..........

7321 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa c tcatcaatg 7380

7321 agtgtttatt tcgtaaaaaa agtgacgtaa gatcaacacc aaacaggttt gagtagttac 7380

.......... ......... SV40_PA_term ......... EBV_r ..........

.......... .......... .......... .......... .......... ..........

7381 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 7440

7381 atagaatagt acagaccgag atcgataggg cggggattga ggcgggtcaa ggcgggtaag 7440

.......... .......... .......... ......... SV40_origin .........

.......... .......... .......... .......... .......... ..........

7441 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 7500

7441 aggcggggta ccgactgatt aaaaaaaata aatacgtctc cggctccggc ggagccggag 7500

.......... ....... SV40_origin SV40pro_F_pri ....... ..........

.......... .......... .......... .......... .......... ..........

7501 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcgtcgagac 7560

7501 actcgataag gtcttcatca ctcctccgaa aaaacctccg gatccgaaaa cgcagctctg 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa 7620

7561 catgggttaa gcgggatatc actcagcata atgcgcgcga gtgaccggca gcaaaatgtt 7620

.......... .......... T7_pro .... M13_f20_pri M13_pUC_f_pri lacZ_a

.......... .......... .......... .......... .......... ..........

7621 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct 7680

7621 gcagcactga cccttttggg accgcaatgg gttgaattag cggaacgtcg tgtaggggga 7680

M13_pUC_f_pri ...... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc 7740

7681 aagcggtcga ccgcattatc gcttctccgg gcgtggctag cgggaagggt tgtcaacgcg 7740

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg 7800

7741 tcggacttac cgcttaccgc gctgcgcggg acatcgccgc gtaattcgcg ccgcccacac 7800

lacZ_a .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

7801 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct 7860

7801 caccaatgcg cgtcgcactg gcgatgtgaa cggtcgcggg atcgcgggcg aggaaagcga 7860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg 7920

7861 aagaagggaa ggaaagagcg gtgcaagcgg ccgaaagggg cagttcgaga tttagccccc 7920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag 7980

7921 gagggaaatc ccaaggctaa atcacgaaat gccgtggagc tggggttttt tgaactaatc 7980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg 8040

7981 ccactaccaa gtgcatcacc cggtagcggg actatctgcc aaaaagcggg aaactgcaac 8040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc 8100

8041 ctcaggtgca agaaattatc acctgagaac aaggtttgac cttgttgtga gttgggatag 8100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat 8160

8101 agccagataa gaaaactaaa tattccctaa aacggctaaa gccggataac caatttttta 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc 8220

8161 ctcgactaaa ttgtttttaa attgcgctta aaattgtttt ataattgcaa atgttaaagg 8220

.......... .......... .......... .......... .......... ..........