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FHRE-Luc
Gene/insert name: FHRE
Alternative names: FKHRL1
FOXO3a
forkhead responsive element
Insert size (bp): 96
Gene/insert aliases: FOXO3, FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677
Species of gene(s): H. sapiens (human)
Relevant mutations/deletions: 3 copies of FRE
Vector backbone: pGL3 basic (Search Vector Database)
Backbone manufacturer: Promega
Type of vector: Mammalian expression,Luciferase
Backbone size (bp): 5094
Cloning site 5'': NheI
Site destroyed during cloning: No
Cloning site 3'': BglII
Site destroyed during cloning: No
5'' Sequencing primer: RVprimer3 (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Michael Greenberg
Article: Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Brunet A et al. (Cell 1999 Mar 19;96(6):857-68. Pubmed)