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    Plasmid map of FHRE-Luc

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    Gene/insert name: FHRE
    Alternative names: FKHRL1
    forkhead responsive element
    Insert size (bp): 96
    Gene/insert aliases: FOXO3, FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677
    Species of gene(s): H. sapiens (human)
    Relevant mutations/deletions: 3 copies of FRE
    Vector backbone: pGL3 basic (Search Vector Database)
    Backbone manufacturer: Promega
    Type of vector: Mammalian expression,Luciferase
    Backbone size (bp): 5094
    Cloning site 5'': NheI
    Site destroyed during cloning: No
    Cloning site 3'': BglII
    Site destroyed during cloning: No
    5'' Sequencing primer: RVprimer3 (List of Sequencing Primers)
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Plasmid Provided In: DH5a
    Principal Investigator: Michael Greenberg
    Article: Akt promotes cell survival by phosphorylating and inhibiting a Forkhead transcription factor. Brunet A et al. (Cell 1999 Mar 19;96(6):857-68. Pubmed)

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