Sequence of FHRE-Luc

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Sequence of FHRE-Luc

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Sequence of FHRE-Luc : 5094 BP; 1331 A; 1220 C; 1210 G; 1333 T; 0 other;

KpnI(5) SacI(11) .......... .......... .......... ..........

1 ggtaccgagc tcttacgcgt gctagcgtga cagagtgaga ctctgtctct atttaaataa 60

1 ccatggctcg agaatgcgca cgatcgcact gtctcactct gagacagaga taaatttatt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... BglII(120)

61 ataagtaaat aaataaactg ggcaaacaat gaaaatgaaa acattgtgaa atacaaagca 120

61 tattcattta tttatttgac ccgtttgtta cttttacttt tgtaacactt tatgtttcgt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gatctgcgat ctgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat 180

121 ctagacgcta gacgtagagt taatcagtcg ttggtatcag ggcggggatt gaggcgggta 180

.......... .......... .......... SV40_pro .......... ..........

.......... .......... .......... .......... .......... ..........

181 cccgccccta actccgccca gttccgccca ttctccgccc catcgctgac taattttttt 240

181 gggcggggat tgaggcgggt caaggcgggt aagaggcggg gtagcgactg attaaaaaaa 240

.......... ..... SV40_origin SV40_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 300

241 ataaatacgt ctccggctcc ggcggagccg gagactcgat aaggtcttca tcactcctcc 300

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... StuI(312) .......... .......... .......... ..........

301 cttttttgga ggcctaggct tttgcaaaaa gcttggcatt ccggtactgt tggtaaagcc 360

301 gaaaaaacct ccggatccga aaacgttttt cgaaccgtaa ggccatgaca accatttcgg 360

.......... SV40_pro .......... .......... .......... ..........

NcoI(362) .......... .......... NarI(397) .......... ..........

361 accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 420

361 tggtaccttc tgcggttttt gtatttcttt ccgggccgcg gtaagatagg cgaccttcta 420

.......... .......... ... ORF 1 firefly_Luciferase ... ..........

.......... .......... .......... .......... .......... ..........

421 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 480

421 ccttggcgac ctctcgttga cgtattccga tacttctcta tgcgggacca aggaccttgt 480

.. LucNrev_pri ORF 1 firefly_Luciferase .. .......... ..........

.......... .......... .......... .......... .......... BstBI(533)

481 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 540

481 taacgaaaat gtctacgtgt atagctccac ctgtagtgaa tgcgactcat gaagctttac 540

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 600

541 aggcaagcca accgtcttcg atactttgct atacccgact tatgtttagt gtcttagcag 600

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 660

601 catacgtcac ttttgagaga agttaagaaa tacggccaca acccgcgcaa taaatagcct 660

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 720

661 caacgtcaac gcgggcgctt gctgtaaata ttacttgcac ttaacgagtt gtcatacccg 720

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

721 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 780

721 taaagcgtcg gatggcacca caagcaaagg tttttcccca acgtttttta aaacttgcac 780

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

781 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 840

781 gtttttttcg agggttagta ggttttttaa taatagtacc taagattttg cctaatggtc 840

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 900

841 cctaaagtca gctacatgtg caagcagtgt agagtagatg gagggccaaa attacttatg 900

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... BclI(944) ..........

901 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 960

901 ctaaaacacg gtctcaggaa gctatccctg ttctgttaac gtgactagta cttgaggaga 960

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 1020

961 cctagatgac cagacggatt tccacagcga gacggagtat cttgacggac gcactctaag 1020

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 1080

1021 agcgtacggt ctctaggata aaaaccgtta gtttagtaag gcctatgacg ctaaaattca 1080

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 1140

1081 caacaaggta aggtagtgcc aaaaccttac aaatgatgtg agcctataaa ctatacacct 1140

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 1200

1141 aaagctcagc agaattacat atctaaactt cttctcgaca aagactcctc ggaagtccta 1200

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 1260

1201 atgttctaag tttcacgcga cgaccacggt tgggataaga ggaagaagcg gttttcgtga 1260

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 1320

1261 gactaactgt ttatgctaaa tagattaaat gtgctttaac gaagaccacc gcgaggggag 1320

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 1380

1321 agattccttc agccccttcg ccaacggttc tccaaggtag acggtccata gtccgttcct 1380

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 1440

1381 atacccgagt gactctgatg tagtcgataa gactaatgtg ggctccccct actatttggc 1440

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 1500

1441 ccgcgccagc catttcaaca aggtaaaaaa cttcgcttcc aacacctaga cctatggccc 1500

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 1560

1501 ttttgcgacc cgcaattagt ttctccgctt gacacacact ctccaggata ctaatacagg 1560

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 1620

1561 ccaatacatt tgttaggcct tcgctggttg cggaactaac tgttcctacc taccgatgta 1620

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 1680

1621 agacctctgt atcgaatgac cctgcttctg cttgtgaaga agtagcaact ggcggacttc 1680

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 1740

1681 agagactaat tcatgtttcc gatagtccac cgagggcgac ttaaccttag gtagaacgag 1740

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 1800

1741 gttgtggggt tgtagaagct gcgtccacag cgtccagaag ggctgctact gcggccactt 1800

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 1860

1801 gaagggcggc ggcaacaaca aaacctcgtg cctttctgct actgcctttt tctctagcac 1860

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 1920

1861 ctaatgcagc ggtcagttca ttgttggcgc tttttcaacg cgcctcctca acacaaacac 1920

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 1980

1921 ctgcttcatg gctttccaga atggcctttt gagctgcgtt ctttttagtc tctctaggag 1980

.......... ... ORF 1 firefly_Luciferase ... .......... ..........

.......... .......... .......... XbaI(2018) .......... ..........

1981 ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agagtcgggg cggccggccg 2040

1981 tatttccggt tcttcccgcc tttctagcgg cacattaaga tctcagcccc gccggccggc 2040

... ORF 1 firefly_Luciferase ... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag 2100

2041 gaagctcgtc tgtactattc tatgtaacta ctcaaacctg tttggtgttg atcttacgtc 2100

.......... .......... ... SV40_PA_term EBV_rev_pri ... ..........

.......... .......... .......... .......... .......... ..........

2101 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata 2160

2101 acttttttta cgaaataaac actttaaaca ctacgataac gaaataaaca ttggtaatat 2160

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg 2220

2161 tcgacgttat ttgttcaatt gttgttgtta acgtaagtaa aatacaaagt ccaagtcccc 2220

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa aatcgataag 2280

2221 ctccacaccc tccaaaaaat ttcgttcatt ttggagatgt ttacaccatt ttagctattc 2280

.......... .......... .......... .......... .......... ..........

SalI(2286) .......... .......... .......... .......... ..........

2281 gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg 2340

2281 ctaggcagct ggctacggga actctcggaa gttgggtcag tcgaggaagg ccacccgcgc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca 2400

2341 cccgtactga tagcagcggc gtgaatactg acagaagaaa tagtacgttg agcatcctgt 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 2460

2401 ccacggccgt cgcgagaagg cgaaggagcg agtgactgag cgacgcgagc cagcaagccg 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 2520

2461 acgccgctcg ccatagtcga gtgagtttcc gccattatgc caataggtgt cttagtcccc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 2580

2521 tattgcgtcc tttcttgtac actcgttttc cggtcgtttt ccggtccttg gcatttttcc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 2640

2581 ggcgcaacga ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg 2640

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2641 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 2700

2641 cgagttcagt ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 2760

2701 cttcgaggga gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 2820

2761 aagagggaag cccttcgcac cgcgaaagag ttacgagtgc gacatccata gagtcaagcc 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 2880

2821 acatccagca agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 2940

2881 cgcggaatag gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 3000

2941 accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 3060

3001 agaacttcac caccggattg atgccgatgt gatcttcctg tcataaacca tagacgcgag 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 3120

3061 acgacttcgg tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 3180

3121 ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 3240

3181 gagttcttct aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg 3240

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 3300

3241 caattcccta aaaccagtac tctaatagtt tttcctagaa gtggatctag gaaaatttaa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 3360

3301 tttttacttc aaaatttagt tagatttcat atatactcat ttgaaccaga ctgtcaatgg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 3420

3361 ttacgaatta gtcactccgt ggatagagtc gctagacaga taaagcaagt aggtatcaac 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 3480

3421 ggactgaggg gcagcacatc tattgatgct atgccctccc gaatggtaga ccggggtcac 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 3540

3481 gacgttacta tggcgctctg ggtgcgagtg gccgaggtct aaatagtcgt tatttggtcg 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 3600

3541 gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa taggcggagg taggtcagat 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

AseI(3601) .......... .......... .......... .......... ..........

3601 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 3660

3601 aattaacaac ggcccttcga tctcattcat caagcggtca attatcaaac gcgttgcaac 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 3720

3661 aacggtaacg atgtccgtag caccacagtg cgagcagcaa accataccga agtaagtcga 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 3780

3721 ggccaagggt tgctagttcc gctcaatgta ctagggggta caacacgttt tttcgccaat 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 3840

3781 cgaggaagcc aggaggctag caacagtctt cattcaaccg gcgtcacaat agtgagtacc 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 3900

3841 aataccgtcg tgacgtatta agagaatgac agtacggtag gcattctacg aaaagacact 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 3960

3901 gaccactcat gagttggttc agtaagactc ttatcacata cgccgctggc tcaacgagaa 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4020

3961 cgggccgcag ttatgcccta ttatggcgcg gtgtatcgtc ttgaaatttt cacgagtagt 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 4080

4021 aaccttttgc aagaagcccc gcttttgaga gttcctagaa tggcgacaac tctaggtcaa 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 4140

4081 gctacattgg gtgagcacgt gggttgacta gaagtcgtag aaaatgaaag tggtcgcaaa 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 4200

4141 gacccactcg tttttgtcct tccgttttac ggcgtttttt cccttattcc cgctgtgcct 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 4260

4201 ttacaactta tgagtatgag aaggaaaaag ttataataac ttcgtaaata gtcccaataa 4260

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 4320

4261 cagagtactc gcctatgtat aaacttacat aaatcttttt atttgtttat ccccaaggcg 4320

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg 4380

4321 cgtgtaaagg ggcttttcac ggtggactgc gcgggacatc gccgcgtaat tcgcgccgcc 4380

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

4381 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 4440

4381 cacaccacca atgcgcgtcg cactggcgat gtgaacggtc gcgggatcgc gggcgaggaa 4440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 4500

4441 agcgaaagaa gggaaggaaa gagcggtgca agcggccgaa aggggcagtt cgagatttag 4500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 4560

4501 cccccgaggg aaatcccaag gctaaatcac gaaatgccgt ggagctgggg ttttttgaac 4560

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 4620

4561 taatcccact accaagtgca tcacccggta gcgggactat ctgccaaaaa gcgggaaact 4620

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 4680

4621 gcaacctcag gtgcaagaaa ttatcacctg agaacaaggt ttgaccttgt tgtgagttgg 4680

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 4740

4681 gatagagcca gataagaaaa ctaaatattc cctaaaacgg ctaaagccgg ataaccaatt 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 4800

4741 ttttactcga ctaaattgtt tttaaattgc gcttaaaatt gttttataat tgcaaatgtt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 tttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt 4860

4801 aaagggtaag cggtaagtcc gacgcgttga caacccttcc cgctagccac gcccggagaa 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgggaggtac 4920

4861 gcgataatgc ggtcgggttc gatggtacta ttcattcatt ataattccat gccctccatg 4920

.......... .......... .......... .......... .......... ..........

NotI(4927) .......... .......... .......... .......... ..........

4921 ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg 4980

4921 aacctcgccg gcgttatttt atagaaataa aagtaatgta gacacacaac caaaaaacac 4980

.......... .......... . synth_PA_term TB up_PA_term . ..........

.......... .......... .......... .......... .......... ..........

4981 tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc 5040

4981 acttagctat catgattgta tgcgagaggt agttttgttt tgctttgttt tgtttgatcg 5040

.......... ........ TB up_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc gata...... 5094

5041 ttttatccga caggggtcac gttcacgtcc acggtcttgt aaagagatag ctat...... 5094

RVpri3_pri ........ TB up_PA_term ........ .......... ..........