Sequence of HI0058

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Sequence of HI0058

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Sequence of HI0058/pET-21a(+)c-tag : 6058 BP; 1480 A; 1500 C; 1600 G; 1478 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .......... XhoI(158) .......... ..........

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagatgtcat ttaccgtgat 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctctacagta aatggcacta 180

T7_term .......... 6xHis .......... .......... ORF 2

.......... .......... .......... .......... .......... ..........

181 tatccccgct cgttttgcat caagtcgttt gccaggaaaa cctcttgctg atattaaagg 240

181 ataggggcga gcaaaacgta gttcagcaaa cggtcctttt ggagaacgac tataatttcc 240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 taagccaatg attcaacatg tatttgagaa agcactgcag tctggggcga gccgagtgat 300

241 attcggttac taagttgtac ataaactctt tcgtgacgtc agaccccgct cggctcacta 300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tattgccacc gataatgaaa atgtggctga tgttgccaaa agttttggtg ctgaagtctg 360

301 ataacggtgg ctattacttt tacaccgact acaacggttt tcaaaaccac gacttcagac 360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tatgacttcg gttaatcata attctggtac agaacgttta gcagaagttg tagaaaaatt 420

361 atactgaagc caattagtat taagaccatg tcttgcaaat cgtcttcaac atctttttaa 420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agctattcct gacaatgaaa tcattgtcaa tattcaaggc gatgagcctt tgattcctcc 480

421 tcgataagga ctgttacttt agtaacagtt ataagttccg ctactcggaa actaaggagg 480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cgttatcgtg cgacaagtgg cagataattt agcaaaattt aatgtcaata tggcaagcct 540

481 gcaatagcac gctgttcacc gtctattaaa tcgttttaaa ttacagttat accgttcgga 540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgcggtaaaa attcacgatg ctgaggaatt atttaatcca aatgcagtga aagtattaac 600

541 acgccatttt taagtgctac gactccttaa taaattaggt ttacgtcact ttcataattg 600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 agataaagat ggctatgtgc tgtatttttc ccgttcggtt attccttatg atcgtgatca 660

601 tctatttcta ccgatacacg acataaaaag ggcaagccaa taaggaatac tagcactagt 660

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gtttatgaat ttgcaggatg ttcagaaagt acagctttct gacgcttact tacgtcatat 720

661 caaatactta aacgtcctac aagtctttca tgtcgaaaga ctgcgaatga atgcagtata 720

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tggcatttac gcatatcgtg cgggtttcat aaaacaatat gtgcaatggg caccgactca 780

721 accgtaaatg cgtatagcac gcccaaagta ttttgttata cacgttaccc gtggctgagt 780

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 acttgaaaat ctagaaaaac ttgagcagct tcgtgtgtta tataacggcg aacgtattca 840

781 tgaactttta gatctttttg aactcgtcga agcacacaat atattgccgc ttgcataagt 840

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cgtagaactt gcgaaagaag tgcctgcagt gggggtggat accgccgaag atttggaaaa 900

841 gcatcttgaa cgctttcttc acggacgtca cccccaccta tggcggcttc taaacctttt 900

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agtgcgggca attttagcgg cgaattaaat ggctgccgcg cggcaccagg ccgctgctgt 960

901 tcacgcccgt taaaatcgcc gcttaattta ccgacggcgc gccgtggtcc ggcgacgaca 960

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gatgatgatg atgatggctg ctgcccatgg tatatctcct tcttaaagtt aaacaaaatt 1020

961 ctactactac tactaccgac gacgggtacc atatagagga agaatttcaa tttgttttaa 1020

.......... .......... .......... ....... T7_transl_en_RBS .......

.......... .......... .......... .......... .......... ..........

1021 atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc 1080

1021 taaagatctc cccttaacaa taggcgagtg ttaaggggat atcactcagc ataattaaag 1080

.......... .......... lacO .......... T7_pro ..........

BglII(1090) ........ .......... .......... .......... ..........

1081 gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca 1140

1081 cgccctagct ctagagctag gagatgcggc ctgcgtagca ccggccgtag tggccgcggt 1140

.......... .......... .......... ......... tet(564-300) .........

.......... .......... .......... .......... .......... ..........

1141 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc 1200

1141 gtccacgcca acgaccgcgg atatagcggc tgtagtggct accccttcta gcccgagcgg 1200

.......... .. tet(564-300) pBRrevBam_pri .. .......... ..........

.......... .......... .......... .......... .......... ..........

1201 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg 1260

1201 tgaagcccga gtactcgcga acaaagccgc acccatacca ccgtccgggg caccggcccc 1260

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc 1320

1261 ctgacaaccc gcggtagagg aacgtacgtg gtaaggaacg ccgccgccac gagttgccgg 1320

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgagatc 1380

1321 agttggatga tgacccgacg aaggattacg tcctcagcgt attccctctc gcagctctag 1380

.......... ......... tet(564-300) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag 1440

1381 ggcctgtggt agcttaccgc gttttggaaa gcgccatacc gtactatcgc gggccttctc 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc 1500

1441 tcagttaagt cccaccactt acactttggt cattgcaata tgctacagcg tctcatacgg 1500

.......... .......... .......... lacI .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 1560

1501 ccacagagaa tagtctggca aagggcgcac cacttggtcc ggtcggtgca aagacgcttt 1560

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 1620

1561 tgcgcccttt ttcaccttcg ccgctaccgc ctcgacttaa tgtaagggtt ggcgcaccgt 1620

.......... .......... lacI .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

1621 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 1680

1621 gttgttgacc gcccgtttgt cagcaacgac taaccgcaac ggtggaggtc agaccgggac 1680

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 1740

1681 gtgcgcggca gcgtttaaca gcgccgctaa tttagagcgc ggctagttga cccacggtcg 1740

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 1800

1741 caccaccaca gctaccatct tgcttcgccg cagcttcgga catttcgccg ccacgtgtta 1800

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 1860

1801 gaagagcgcg ttgcgcagtc acccgactag taattgatag gcgacctact ggtcctacgg 1860

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 1920

1861 taacgacacc ttcgacggac gtgattacaa ggccgcaata aagaactaca gagactggtc 1920

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 1980

1921 tgtgggtagt tgtcataata aaagagggta cttctgccat gcgctgaccc gcacctcgta 1980

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... ApaI(2023) ..........

1981 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 2040

1981 gaccagcgta acccagtggt cgtttagcgc gacaatcgcc cgggtaattc aagacagagc 2040

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 2100

2041 cgcgcagacg cagaccgacc gaccgtattt atagagtgag cgttagttta agtcggctat 2100

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 2160

2101 cgccttgccc ttccgctgac ctcacggtac aggccaaaag ttgtttggta cgtttacgac 2160

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 2220

2161 ttactcccgt agcaagggtg acgctacgac caacggttgc tagtctaccg cgacccgcgt 2220

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... ......... EcoRV(2262) .........

2221 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 2280

2221 tacgcgcggt aatggctcag gcccgacgcg caaccacgcc tatagagcca tcaccctatg 2280

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... HpaI(2318) .......... ..........

2281 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 2340

2281 ctgctatggc ttctgtcgag tacaatatag ggcggcaatt ggtggtagtt tgtcctaaaa 2340

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 2400

2341 gcggacgacc ccgtttggtc gcacctggcg aacgacgttg agagagtccc ggtccgccac 2400

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 2460

2401 ttcccgttag tcgacaacgg gcagagtgac cacttttctt tttggtggga ccgcgggtta 2460

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 2520

2461 tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg tgctgtccaa 2520

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtaagttagc tcactcatta 2580

2521 agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cattcaatcg agtgagtaat 2580

.......... lacI ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggcaccggga tctcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg 2640

2581 ccgtggccct agagctggct acgggaactc tcggaagttg ggtcagtcga ggaaggccac 2640

.......... .......... .......... ......... tet(852-576) .........

.......... .......... .......... .......... .......... ..........

2641 ggcgcggggc atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt 2700

2641 ccgcgccccg tactgatagc agcggcgtga atactgacag aagaaatagt acgttgagca 2700

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aggacaggtg ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc 2760

2701 tcctgtccac ggccgtcgcg agacccagta aaagccgctc ctggcgaaag cgacctcgcg 2760

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gacgatgatc ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt 2820

2761 ctgctactag ccggacagcg aacgccataa gccttagaac gtgcgggagc gagttcggaa 2820

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc 2880

2821 gcagtgacca gggcggtggt ttgcaaagcc gctcttcgtc cggtaatagc ggccgtaccg 2880

.......... ......... tet(852-576) ......... .......... ..........

.......... FspI(2894) .......... .......... .......... ..........

2881 ggccccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag gctggcgggg 2940

2881 ccggggtgcc cacgcgtact agcacgagga cagcaactcc tgggccgatc cgaccgcccc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg 3000

2941 aacggaatga ccaatcgtct tacttagtgg ctatgcgctc gcttgcactt cgctgacgac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta 3060

3001 gacgttttgc agacgctgga ctcgttgttg tacttaccag aagccaaagg cacaaagcat 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct gcatcgcagg 3120

3061 ttcagacctt tgcgccttca gtcgcgggac gtggtaatac aaggcctaga cgtagcgtcc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct ggcattgacc 3180

3121 tacgacgacc gatgggacac cttgtggatg tagacataat tgcttcgcga ccgtaactgg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac cctcacaacg 3240

3181 gactcactaa aaagagacca gggcggcgta ggtatggcgg tcaacaaatg ggagtgttgc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc tctctcgttt 3300

3241 aaggtcattg gcccgtacaa gtagtagtca ttgggcatag cactcgtagg agagagcaaa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat cagtgaccaa 3360

3301 gtagccatag taatgggggt acttgtcttt agggggaatg tgcctccgta gtcactggtt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat taacgcttct 3420

3361 tgtccttttt tggcgggaat tgtaccgggc gaaatagtct tcggtctgta attgcgaaga 3420

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat cgcttcacga 3480

3421 cctctttgag ttgctcgacc tgcgcctact tgtccgtctg tagacactta gcgaagtgct 3480

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg gtgaaaacct 3540

3481 ggtgcgacta ctcgaaatgg cgtcgacgga gcgcgcaaag ccactactgc cacttttgga 3540

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 3600

3541 gactgtgtac gtcgagggcc tctgccagtg tcgaacagac attcgcctac ggccctcgtc 3600

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca 3660

3601 tgttcgggca gtcccgcgca gtcgcccaca accgcccaca gccccgcgtc ggtactgggt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta 3720

3661 cagtgcatcg ctatcgcctc acatatgacc gaattgatac gccgtagtct cgtctaacat 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 3780

3721 gactctcacg tggtatatac gccacacttt atggcgtgtc tacgcattcc tcttttatgg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3840

3781 cgtagtccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 3900

3841 ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 3960

3901 tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca tttttccggc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 4020

3961 gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 4080

4021 gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 4140

4081 cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 4200

4141 agggaagccc ttcgcaccgc gaaagagtat cgagtgcgac atccatagag tcaagccaca 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 4260

4201 tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 4320

4261 ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 4380

4321 gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc 4440

4381 acttcaccac cggattgatg ccgatgtgat cttcctgtca taaaccatag acgcgagacg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 4500

4441 acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 4560

4501 gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt tttcctagag 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 4620

4561 ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt ttgagtgcaa 4620

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 aagggatttt ggtcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt 4680

4621 ttccctaaaa ccagtacttg ttattttgac agacgaatgt atttgtcatt atgttcccca 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gttatgagcc atattcaacg ggaaacgtct tgctctaggc cgcgattaaa ttccaacatg 4740

4681 caatactcgg tataagttgc cctttgcaga acgagatccg gcgctaattt aaggttgtac 4740

.......... .......... ......... ORF 1 KanR2 ......... ..........

.......... .......... .......... NruI(4772) .......... ..........

4741 gatgctgatt tatatgggta taaatgggct cgcgataatg tcgggcaatc aggtgcgaca 4800

4741 ctacgactaa atatacccat atttacccga gcgctattac agcccgttag tccacgctgt 4800

.......... ......... ORF 1 KanR2 ......... .......... ..........

ClaI(4806) .......... .......... .......... .......... ..........

4801 atctatcgat tgtatgggaa gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt 4860

4801 tagatagcta acataccctt cgggctacgc ggtctcaaca aagactttgt accgtttcca 4860

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agcgttgcca atgatgttac agatgagatg gtcagactaa actggctgac ggaatttatg 4920

4861 tcgcaacggt tactacaatg tctactctac cagtctgatt tgaccgactg ccttaaatac 4920

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cctcttccga ccatcaagca ttttatccgt actcctgatg atgcatggtt actcaccact 4980

4921 ggagaaggct ggtagttcgt aaaataggca tgaggactac tacgtaccaa tgagtggtga 4980

.......... ......... ORF 1 KanR2 ......... .......... ..........

SmaI(4989) .......... .......... .......... .......... ..........

4981 gcgatccccg ggaaaacagc attccaggta ttagaagaat atcctgattc aggtgaaaat 5040

4981 cgctaggggc ccttttgtcg taaggtccat aatcttctta taggactaag tccactttta 5040

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 attgttgatg cgctggcagt gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt 5100

5041 taacaactac gcgaccgtca caaggacgcg gccaacgtaa gctaaggaca aacattaaca 5100

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc aatcacgaat gaataacggt 5160

5101 ggaaaattgt cgctagcgca taaagcagag cgagtccgcg ttagtgctta cttattgcca 5160

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ttggttgatg cgagtgattt tgatgacgag cgtaatggct ggcctgttga acaagtctgg 5220

5161 aaccaactac gctcactaaa actactgctc gcattaccga ccggacaact tgttcagacc 5220

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aaagaaatgc ataaactttt gccattctca ccggattcag tcgtcactca tggtgatttc 5280

5221 tttctttacg tatttgaaaa cggtaagagt ggcctaagtc agcagtgagt accactaaag 5280

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tcacttgata accttatttt tgacgagggg aaattaatag gttgtattga tgttggacga 5340

5281 agtgaactat tggaataaaa actgctcccc tttaattatc caacataact acaacctgct 5340

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gtcggaatcg cagaccgata ccaggatctt gccatcctat ggaactgcct cggtgagttt 5400

5341 cagccttagc gtctggctat ggtcctagaa cggtaggata ccttgacgga gccactcaaa 5400

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tctccttcat tacagaaacg gctttttcaa aaatatggta ttgataatcc tgatatgaat 5460

5401 agaggaagta atgtctttgc cgaaaaagtt tttataccat aactattagg actatactta 5460

.......... ......... ORF 1 KanR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aaattgcagt ttcatttgat gctcgatgag tttttctaag aattaattca tgagcggata 5520

5461 tttaacgtca aagtaaacta cgagctactc aaaaagattc ttaattaagt actcgcctat 5520

......... ORF 1 KanR2 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 5580

5521 gtataaactt acataaatct ttttatttgt ttatccccaa ggcgcgtgta aaggggcttt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 agtgccacct gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta 5640

5581 tcacggtgga ctttaacatt tgcaattata aaacaatttt aagcgcaatt taaaaacaat 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga 5700

5641 ttagtcgagt aaaaaattgg ttatccggct ttagccgttt tagggaatat ttagttttct 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa 5760

5701 tatctggctc tatcccaact cacaacaagg tcaaaccttg ttctcaggtg ataatttctt 5760

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

5761 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga 5820

5761 gcacctgagg ttgcagtttc ccgctttttg gcagatagtc ccgctaccgg gtgatgcact 5820

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc 5880

5821 tggtagtggg attagttcaa aaaaccccag ctccacggca tttcgtgatt tagccttggg 5880

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga 5940

5881 atttccctcg ggggctaaat ctcgaactgc ccctttcggc cgcttgcacc gctctttcct 5940

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg 6000

5941 tcccttcttt cgctttcctc gcccgcgatc ccgcgaccgt tcacatcgcc agtgcgacgc 6000

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgcca.. 6058

6001 gcattggtgg tgtgggcggc gcgaattacg cggcgatgtc ccgcgcaggg taagcggt.. 6058

.......... f1_origin .......... .......... .......... ..........