[.[."LOCUS AY773970 4924 bp DNA circular SYN 01-DEC-2004 DEFINITION Expression vector pHT2, complete sequence. ACCESSION AY773970 VERSION AY773970.1 GI:56089682 KEYWORDS . SOURCE Expression vector pHT2 ORGANISM Expression vector pHT2 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4924) AUTHORS Los,G. and Zimprich,C. TITLE Direct Submission JOURNAL Submitted (08-OCT-2004) Scientific Communications, Promega Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA FEATURES Location/Qualifiers source 1..4924 /organism="Expression vector pHT2" /mol_type="other DNA" /db_xref="taxon:301958" promoter 1..742 /note="CMV enhancer/promoter" misc_feature 857..989 /note="chimeric intron" promoter 1034..1052 /note="T7" CDS 1081..1971 /codon_start=1 /transl_table=11 /product="HT2" /protein_id="AAV70825.1" /db_xref="GI:56089683" /translation="MGSEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPT SSYLWRNIIPHVAPSHRCIAPDLIGMGKSDKPDLDYFFDDHVRYLDAFIEALGLEEVV LVIHDWGSALGFHWAKRNPERVKGIACMEFIRPIPTWDEWPEFARETFQAFRTADVGR ELIIDQNAFIEGALPMGVVRPLTEVEMDHYREPFLKPVDREPLWRFPNELPIAGEPAN IVALVEAYMNWLHQSPVPKLLFWGTPGVLIPPAEAARLAESLPNCKTVDIGPGLFLLQ EDNPDLIGSEIARWLPGLAG" polyA_signal 2024..2245 /note="SV40" CDS 3232..4092 /note="AmpR" /codon_start=1 /transl_table=11 /product="beta-lactamase" /protein_id="AAV70826.1" /db_xref="GI:56089684" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4818..4854 ORIGIN 1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc 241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 1021 aggctagagt acttaatacg actcactata ggctagccag ctggcgcgga tatcgccacc 1081 atgggatccg aaatcggtac aggcttcccc ttcgaccccc attatgtgga agtcctgggc 1141 gagcgtatgc actacgtcga tgttggaccg cgggatggca cgcctgtgct gttcctgcac 1201 ggtaacccga cctcgtccta cctgtggcgc aacatcatcc cgcatgtagc accgagtcat 1261 cggtgcattg ctccagacct gatcgggatg ggaaaatcgg acaaaccaga cctcgattat 1321 ttcttcgacg accacgtccg ctacctcgat gccttcatcg aagccttggg tttggaagag 1381 gtcgtcctgg tcatccacga ctggggctca gctctcggat tccactgggc caagcgcaat 1441 ccggaacggg tcaaaggtat tgcatgtatg gaattcatcc ggcctatccc gacgtgggac 1501 gaatggccag aattcgcccg tgagaccttc caggccttcc ggaccgccga cgtcggccga 1561 gagttgatca tcgatcagaa cgctttcatc gagggtgcgc tcccgatggg ggtcgtccgt 1621 ccgcttacgg aggtcgagat ggaccactat cgcgagccct tcctcaagcc tgttgaccga 1681 gagccactgt ggcgattccc caacgagctg cccatcgccg gtgagcccgc gaacatcgtc 1741 gcgctcgtcg aggcatacat gaactggctg caccagtcac ctgtcccgaa gttgttgttc 1801 tggggcacac ccggcgtact gatccccccg gccgaagccg cgagacttgc cgaaagcctc 1861 cccaactgca agacagtgga catcggcccg ggattgttct tgctccagga agacaacccg 1921 gaccttatcg gcagtgagat cgcgcgctgg ctccccgggc tggccggcta atagttaatt 1981 aagtaggcgg ccgcttccct ttagtgaggg ttaatgcttc gagcagacat gataagatac 2041 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 2101 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 2161 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc 2221 aagtaaaacc tctacaaatg tggtaaaatc cgataaggat cgatccgggc tggcgtaata 2281 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga 2341 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 2401 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 2461 gttcgcgggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 2521 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 2581 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 2641 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 2701 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 2761 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg 2821 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 2881 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 2941 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 3001 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 3061 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 3121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 3181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 3241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 3301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 3361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 3421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 3481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 3541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 3601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 3661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 3721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 3781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 3841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 3901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 3961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 4021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 4081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 4141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 4201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 4261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 4321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 4381 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 4441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 4501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 4561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 4621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 4681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 4741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 4801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 4861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag 4921 atct //< HaloTag pHT2 "Times New Roman[.C<CAG_en"Times New Roman[.C< CMV_imm_pro"Times New Roman[.<CMV_pro"Times New Roman3[.CɆ< CMV_f_pri"Times New Roman[.C< pCEP_f_pri"Times New Roman[.C< LNCX_primer"Times New Roman[.CɆ<Ychim_int"Times New Roman[.< T7_pro"Times New Roman[.C<9 Orf frame 1"Times New Roman<[.CɆ<e SV40_PA_term"Times New Roman<[.C< EBV_rev_pri"Times New Roman[.<5 g  f1_origin"Times New Roman[.C<  pGEX_3_primer"Times New Roman[.C<Z v  AmpR_promoter"Times New Roman[.CɆ<  Orf frame 1"Times New Roman<[.<  Ampicillin"Times New Roman[.C< pBR322_origin"Times New Roman [.SpeI"Times New Roman <qbq [."SacI(721), HindIII(748), PstI(830)"Times New Roman.!<3Zii [.1NheI(1052), PvuII(1060), EcoRV(1071), BamHI(1084)"Times New Roman/<U6i2m3mQ [.StuI(1534), BclI(1565)"Times New Roman<K]gg [.NruI"Times New Romant %<>O`` 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