Sequence of HaloTag

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

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Sequence of HaloTag

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Sequence of HaloTag pHT2 : 4924 BP; 1199 A; 1241 C; 1216 G; 1268 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta 60

1 agttataacc ggtaatcggt ataataagta accaatatat cgtatttagt tataaccgat 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc 120

61 aaccggtaac gtatgcaaca tagatatagt attatacatg taaatataac cgagtacagg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... SpeI(152) .......... ..........

121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg 180

121 ttatactggc ggtacaaccg taactaataa ctgatcaata attatcatta gttaatgccc 180

.......... .......... .......... .......... ......... CMV_imm_pro

.......... .......... .......... .......... .......... ..........

181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg ta aatggccc 240

181 cagtaatcaa gtatcgggta tatacctcaa ggcgcaatgt attgaatgcc atttaccggg 240

.......... ......... CMV_imm_pro ......... CAG_en ..........

.......... .......... .......... .......... .......... ..........

241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat 300

241 cggaccgact ggcgggttgc tgggggcggg taactgcagt tattactgca tacaagggta 300

.......... ...... CAG_en CMV_imm_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc 360

301 tcattgcggt tatccctgaa aggtaactgc agttacccac ctcataaatg ccatttgacg 360

.......... ...... CAG_en CMV_imm_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga 420

361 ggtgaaccgt catgtagttc acatagtata cggttcaggc gggggataac tgcagttact 420

.......... ...... CAG_en CMV_imm_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg 480

421 gccatttacc gggcggaccg taatacgggt catgtactgg aatgccctga aaggatgaac 480

.......... ...... CAG_en CMV_imm_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac 540

481 cgtcatgtag atgcataatc agtagcgata atggtaccac tacgccaaaa ccgtcatgtg 540

......... CAG_en CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt 600

541 gttacccgca cctatcgcca aactgagtgc ccctaaaggt tcagaggtgg ggtaactgca 600

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc 660

601 gttaccctca aacaaaaccg tggttttagt tgccctgaaa ggttttacag cattattggg 660

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc 720

661 gcggggcaac tgcgtttacc cgccatccgc acatgccacc ctccagatat attcgtctcg 720

.......... CMV_imm_pro CMV_pro CMV_f_pri .......... ..........

SacI(721) .......... .......... .......... .......... ..........

721 tcgtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat 780

721 agcaaatcac ttggcagtct agtgatcttc gaaataacgc catcaaatag tgtcaattta 780

pCEP_f_pri CMV_pro LNCX_primer .......... .......... ..........

.......... .......... .......... .......... PstI(830) ..........

781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc 840

781 acgattgcgt cagtcacgaa gactgtgttg tcagagcttg aattcgacgt cttcaaccag 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa 900

841 cactccgtga cccgtccatt catagttcca atgttctgtc caaattcctc tggttatctt 900

.......... .......... .......... chim_int .......... ..........

.......... .......... .......... .......... .......... ..........

901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac 960

901 tgacccgaac agctctgtct cttctgagaa cgcaaagact atccgtggat aaccagaatg 960

.......... .......... chim_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta 1020

961 actgtaggtg aaacggaaag agaggtgtcc acaggtgagg gtcaagttaa tgtcgagaat 1020

.......... chim_int .......... .......... .......... ..........

.......... .......... .... NheI(1052) PvuII(1060) .... ..........

1021 aggctagagt acttaatacg actcactata ggctagccag ctggcgcgga tatcgccacc 1080

1021 tccgatctca tgaattatgc tgagtgatat ccgatcggtc gaccgcgcct atagcggtgg 1080

.......... .......... T7_pro .......... .......... ..........

BamHI(1084) ........ .......... .......... .......... ..........

1081 atgggatccg aaatcggtac aggcttcccc ttcgaccccc attatgtgga agtcctgggc 1140

1081 taccctaggc tttagccatg tccgaagggg aagctggggg taatacacct tcaggacccg 1140

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gagcgtatgc actacgtcga tgttggaccg cgggatggca cgcctgtgct gttcctgcac 1200

1141 ctcgcatacg tgatgcagct acaacctggc gccctaccgt gcggacacga caaggacgtg 1200

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggtaacccga cctcgtccta cctgtggcgc aacatcatcc cgcatgtagc accgagtcat 1260

1201 ccattgggct ggagcaggat ggacaccgcg ttgtagtagg gcgtacatcg tggctcagta 1260

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cggtgcattg ctccagacct gatcgggatg ggaaaatcgg acaaaccaga cctcgattat 1320

1261 gccacgtaac gaggtctgga ctagccctac ccttttagcc tgtttggtct ggagctaata 1320

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttcttcgacg accacgtccg ctacctcgat gccttcatcg aagccttggg tttggaagag 1380

1321 aagaagctgc tggtgcaggc gatggagcta cggaagtagc ttcggaaccc aaaccttctc 1380

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtcgtcctgg tcatccacga ctggggctca gctctcggat tccactgggc caagcgcaat 1440

1381 cagcaggacc agtaggtgct gaccccgagt cgagagccta aggtgacccg gttcgcgtta 1440

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccggaacggg tcaaaggtat tgcatgtatg gaattcatcc ggcctatccc gacgtgggac 1500

1441 ggccttgccc agtttccata acgtacatac cttaagtagg ccggataggg ctgcaccctg 1500

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gaatggccag aattcgcccg tgagaccttc caggccttcc ggaccgccga cgtcggccga 1560

1501 cttaccggtc ttaagcgggc actctggaag gtccggaagg cctggcggct gcagccggct 1560

.......... ......... Orf frame 1 ......... .......... ..........

BclI(1565) .......... .......... .......... .......... ..........

1561 gagttgatca tcgatcagaa cgctttcatc gagggtgcgc tcccgatggg ggtcgtccgt 1620

1561 ctcaactagt agctagtctt gcgaaagtag ctcccacgcg agggctaccc ccagcaggca 1620

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... NruI(1652) .......... ..........

1621 ccgcttacgg aggtcgagat ggaccactat cgcgagccct tcctcaagcc tgttgaccga 1680

1621 ggcgaatgcc tccagctcta cctggtgata gcgctcggga aggagttcgg acaactggct 1680

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gagccactgt ggcgattccc caacgagctg cccatcgccg gtgagcccgc gaacatcgtc 1740

1681 ctcggtgaca ccgctaaggg gttgctcgac gggtagcggc cactcgggcg cttgtagcag 1740

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcgctcgtcg aggcatacat gaactggctg caccagtcac ctgtcccgaa gttgttgttc 1800

1741 cgcgagcagc tccgtatgta cttgaccgac gtggtcagtg gacagggctt caacaacaag 1800

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tggggcacac ccggcgtact gatccccccg gccgaagccg cgagacttgc cgaaagcctc 1860

1801 accccgtgtg ggccgcatga ctaggggggc cggcttcggc gctctgaacg gctttcggag 1860

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cccaactgca agacagtgga catcggcccg ggattgttct tgctccagga agacaacccg 1920

1861 gggttgacgt tctgtcacct gtagccgggc cctaacaaga acgaggtcct tctgttgggc 1920

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gaccttatcg gcagtgagat cgcgcgctgg ctccccgggc tggccggcta atagttaatt 1980

1921 ctggaatagc cgtcactcta gcgcgcgacc gaggggcccg accggccgat tatcaattaa 1980

.......... ......... Orf frame 1 ......... .......... ..........

NotI(1988) .......... .......... .......... .......... ..........

1981 aagtaggcgg ccgcttccct ttagtgaggg ttaatgcttc gagcagacat gataagatac 2040

1981 ttcatccgcc ggcgaaggga aatcactccc aattacgaag ctcgtctgta ctattctatg 2040

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

2041 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa 2100

2041 taactactca aacctgtttg gtgttgatct tacgtcactt tttttacgaa ataaacactt 2100

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... HpaI(2154)

2101 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac 2160

2101 taaacactac gataacgaaa taaacattgg taatattcga cgttatttgt tcaattgttg 2160

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc 2220

2161 ttgttaacgt aagtaaaata caaagtccaa gtccccctct acaccctcca aaaaatttcg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aagtaaaacc tctacaaatg tggtaaaatc cgataaggat cgatccgggc tggcgtaata 2280

2221 ttcattttgg agatgtttac accattttag gctattccta gctaggcccg accgcattat 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga 2340

2281 cgcttctccg ggcgtggcta gcgggaaggg ttgtcaacgc gtcggactta ccgcttacct 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 2400

2341 gcgcgggaca tcgccgcgta attcgcgccg cccacaccac caatgcgcgt cgcactggcg 2400

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 2460

2401 atgtgaacgg tcgcgggatc gcgggcgagg aaagcgaaag aagggaagga aagagcggtg 2460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gttcgcgggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag 2520

2461 caagcgcccg aaaggggcag ttcgagattt agcccccgag ggaaatccca aggctaaatc 2520

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 2580

2521 acgaaatgcc gtggagctgg ggttttttga actaatccca ctaccaagtg catcacccgg 2580

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 2640

2581 tagcgggact atctgccaaa aagcgggaaa ctgcaacctc aggtgcaaga aattatcacc 2640

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 2700

2641 tgagaacaag gtttgacctt gttgtgagtt gggatagagc cagataagaa aactaaatat 2700

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 2760

2701 tccctaaaac ggctaaagcc ggataaccaa ttttttactc gactaaattg tttttaaatt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgcgaatttt aacaaaatat taacgcttac aatttcctga tgcggtattt tctccttacg 2820

2761 gcgcttaaaa ttgttttata attgcgaatg ttaaaggact acgccataaa agaggaatgc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc 2880

2821 gtagacacgc cataaagtgt ggcgtatacc acgtgagagt catgttagac gagactacgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 2940

2881 cgtatcaatt cggtcggggc tgtgggcggt tgtgggcgac tgcgcgggac tgcccgaaca 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 3000

2941 gacgagggcc gtaggcgaat gtctgttcga cactggcaga ggccctcgac gtacacagtc 3000

.......... .......... .......... ........ pGEX_3_primer ........

.......... .......... .......... .......... .......... ..........

3001 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt 3060

3001 tccaaaagtg gcagtagtgg ctttgcgcgc tctgctttcc cggagcacta tgcggataaa 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga 3120

3061 aatatccaat tacagtacta ttattaccaa agaatctgca gtccaccgtg aaaagcccct 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 3180

3121 ttacacgcgc cttggggata aacaaataaa aagatttatg taagtttata cataggcgag 3180

.......... .......... .......... .......... ........ AmpR_promoter

.......... .......... .......... .......... .......... ..........

3181 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 3240

3181 tactctgtta ttgggactat ttacgaagtt attataactt tttccttctc atactcataa 3240

AmpR_promoter ...... .......... .......... .... Orf frame 1 Ampicillin

.......... .......... .......... .......... .......... ..........

3241 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 3300

3241 gttgtaaagg cacagcggga ataagggaaa aaacgccgta aaacggaagg acaaaaacga 3300

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 3360

3301 gtgggtcttt gcgaccactt tcattttcta cgacttctag tcaacccacg tgctcaccca 3360

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 3420

3361 atgtagcttg acctagagtt gtcgccattc taggaactct caaaagcggg gcttcttgca 3420

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac 3480

3421 aaaggttact actcgtgaaa atttcaagac gatacaccgc gccataatag ggcataactg 3480

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac 3540

3481 cggcccgttc tcgttgagcc agcggcgtat gtgataagag tcttactgaa ccaactcatg 3540

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 3600

3541 agtggtcagt gtcttttcgt agaatgccta ccgtactgtc attctcttaa tacgtcacga 3600

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 3660

3601 cggtattggt actcactatt gtgacgccgg ttgaatgaag actgttgcta gcctcctggc 3660

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 3720

3661 ttcctcgatt ggcgaaaaaa cgtgttgtac cccctagtac attgagcgga actagcaacc 3720

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 3780

3721 cttggcctcg acttacttcg gtatggtttg ctgctcgcac tgtggtgcta cggacatcgt 3780

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 3840

3781 taccgttgtt gcaacgcgtt tgataattga ccgcttgatg aatgagatcg aagggccgtt 3840

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 3900

3841 gttaattatc tgacctacct ccgcctattt caacgtcctg gtgaagacgc gagccgggaa 3900

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 3960

3901 ggccgaccga ccaaataacg actatttaga cctcggccac tcgcacccag agcgccatag 3960

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 4020

3961 taacgtcgtg accccggtct accattcggg agggcatagc atcaatagat gtgctgcccc 4020

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 4080

4021 tcagtccgtt gatacctact tgctttatct gtctagcgac tctatccacg gagtgactaa 4080

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 4140

4081 ttcgtaacca ttgacagtct ggttcaaatg agtatatatg aaatctaact aaattttgaa 4140

Orf frame 1 Ampicillin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 4200

4141 gtaaaaatta aattttccta gatccacttc taggaaaaac tattagagta ctggttttag 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 4260

4201 ggaattgcac tcaaaagcaa ggtgactcgc agtctggggc atcttttcta gtttcctaga 4260

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

4261 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 4320

4261 agaactctag gaaaaaaaga cgcgcattag acgacgaacg tttgtttttt tggtggcgat 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 4380

4321 ggtcgccacc aaacaaacgg cctagttctc gatggttgag aaaaaggctt ccattgaccg 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac 4440

4381 aagtcgtctc gcgtctatgg tttatgacaa gaagatcaca tcggcatcaa tccggtggtg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 4500

4441 aagttcttga gacatcgtgg cggatgtatg gagcgagacg attaggacaa tggtcaccga 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 4560

4501 cgacggtcac cgctattcag cacagaatgg cccaacctga gttctgctat caatggccta 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 4620

4561 ttccgcgtcg ccagcccgac ttgcccccca agcacgtgtg tcgggtcgaa cctcgcttgc 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 4680

4621 tggatgtggc ttgactctat ggatgtcgca ctcgatactc tttcgcggtg cgaagggctt 4680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 4740

4681 ccctctttcc gcctgtccat aggccattcg ccgtcccagc cttgtcctct cgcgtgctcc 4740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 4800

4741 ctcgaaggtc cccctttgcg gaccatagaa atatcaggac agcccaaagc ggtggagact 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc 4860

4801 gaactcgcag ctaaaaacac tacgagcagt ccccccgcct cggatacctt tttgcggtcg 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... ......... BglII(4919)

4861 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat ggctcgacag 4920

4861 ttgcgccgga aaaatgccaa ggaccggaaa acgaccggaa aacgagtgta ccgagctgtc 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 atct...... .......... .......... .......... .......... .......... 4924

4921 taga...... .......... .......... .......... .......... .......... 4924

.......... .......... .......... .......... .......... ..........