u u#1 aagcttgcatgcctgcaggtcgactctaggcacataaagaaaaacataac 51 taaccaagctgcagccgagacagtgaaaagaaccgttaaaacggtttgtt 101 ttaaataaactgaattatttagagtcatttctttggtaggaaagtacatt 151 ggcacgtaaaggagcccaaagcaatctgtggaaagcccaggctgggagcc 201 cagcagtttgcatcccctcctggcgtgtacctaagggtttcttaattgtg 251 tggtttctaaatcttccagagggtttgtctcattcacttccacttcggtg 301 cacaatacttggacgcggatttactgtcttagcatctatcggtggccctt 351 cgattgaggctgaacctgaggcccacttcttcagcttgttaaggagagca 401 caagcaccagaagaggctgacccggcagacctgtgggcatttttaacaag 451 ggcctcctgggtctgtgggaggcaggcttacataaggtgcaaattagaaa 501 tataaataataagcccatatcaatttgtcatctttttttaagctcaagtt 551 ttgaaagaccccacctgtaggtttggcaagctagcttaagtaacgccatt 601 ttgcaaggcatggaaaatacataactgagaatagagaagttcagatcaag 651 gttaggaacagagagacagcagaatatgggccaaacaggatatctgtggt 701 aagcagttcctgccccgctcagggccaagaacagttggaacaggagaata 751 tgggccaaacaggatatctgtggtaagcagttcctgccccggctcagggc 801 caagaacagatggtccccagatgcggtcccgccctcagcagtttctagag 851 aaccatcagatgtttccagggtgccccaaggacctgaaatgaccctgtgc 901 cttatttgaactaaccaatcagttcgcttctcgcttctgttcgcgcgctt 951 ctgctccccgagctcaataaaagagcccacaacccctcactcggcgcgcc 1001 agtcctccgatagactgcgtcgcccgggtacccgtgttctcaataaaccc 1051 tcttgcagttgcatccgactcgtggtctcgctgttccttgggagggtctc 1101 ctctgagtgattgactacccgtcagcggggtctttcatttggaggttcca 1151 ccgagatttggagacccctgcccagggaccaccgacccccccgccgggag 1201 gtaagctggccagcaacttatctgtgtctgtccgattgtctagtgtctat 1251 gactgattttatgcgcctgcgtcggtactagttagctaactagctctgta 1301 tctggcggacccgtggtggaactgacgagttcggaacacccggccgcaac 1351 cctgggagacgtcccagggacttcgggggccgtttttgtggcccgacctg 1401 agtcctaaaatcccgatcgtttaggactctttggtgcaccccccttagag 1451 gagggatatgtggttctggtaggagacgagaacctaaaacagttcccgcc 1501 tccgtctgaatttttgctttcggtttgggaccgaagccgcgccgcgcgtc 1551 ttgtctgctgcagcatcgttctgtgttgtctctgtctgactgtgtttctg 1601 tatttgtctgaaaatatgggcccgggctagactgttaccactcccttaag 1651 tttgaccttaggtcactggaaagatgtcgagcggatcgctcacaaccagt 1701 cggtagatgtcaagaagagacgttgggttaccttctgctctgcagaatgg 1751 ccaacctttaacgtcggatggccgcgagacggcacctttaaccgagacct 1801 catcacccaggttaagatcaaggtcttttcacctggcccgcatggacacc 1851 cagaccaggtcccctacatcgtgacctgggaagccttggcttttgacccc 1901 cctccctgggtcaagccctttgtacaccctaagcctccgcctcctcttcc 1951 tccatccgccccgtctctcccccttgaacctcctcgttcgaccccgcctc 2001 gatcctccctttatccagccctcactccttctctaggcgcccccatatgg 2051 ccatatgagatcttatatggggcacccccgccccttgtaaacttccctga 2101 ccctgacatgacaagagttactaacagcccctctctccaagctcacttac 2151 aggctctctacttagtccagcacgaagtctggagacctctggcggcagcc 2201 taccaagaacaactggaccgaccggtggtacctcacccttaccgagtcgg 2251 cgacacagtgtgggtccgccgacaccagactaagaacctagaacctcgct 2301 ggaaaggaccttacacagtcctgctgaccacccccaccgccctcaaagta 2351 gacggcatcgcagcttggatacacgccgcccacgtgaaggctgccgaccc 2401 cgggggtggaccatcctctagactgccatggccgagtacaagcccacggt 2451 gcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccg 2501 ccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgc 2551 cacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgg 2601 gctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcgg 2651 tctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatc 2701 ggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaaca 2751 gatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcc 2801 tggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagc 2851 gccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgc 2901 cttcctggagacctccgcgccccgcaacctccccttctacgagcggctcg 2951 gcttcaccgtcaccgccgacgtcgagtgcccgaaggaccgcgcgacctgg 3001 tgcatgacccgcaagcccggtgcctgacgcggccgcctccggccattagc 3051 catattattcattggttatatagcataaatcaatattggctattggccat 3101 tgcatacgttgtatccatatcataatatgtacatttatattggctcatgt 3151 ccaacattaccgccatgttgacattgattattgactagttattaatagta 3201 atcaattacggggtcattagttcatagcccatatatggagttccgcgtta 3251 cataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgc 3301 ccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggac 3351 tttccattgacgtcaatgggtggagtatttacggtaaactgcccacttgg 3401 cagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaat 3451 gacggtaaatggcccgcctggcattatgcccagtacatgaccttatggga 3501 ctttcctacttggcagtacatctacgtattagtcatcgctattaccatgg 3551 tgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactca 3601 cggggatttccaagtctccaccccattgacgtcaatgggagtttgttttg 3651 gcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccat 3701 tgacgcaaatgggcggtaggcatgtacggtgggaggtctatataagcaga 3751 gctcgtttagtgaaccgtcagatcgcctggagacgccatccacgctgttt 3801 tgacctccatagaagacaccgggaccgatccagcctccgcggccccaagc 3851 ttataacttcgtatagcatacattatacgaagttatagatccaatattat 3901 tgaagcatttatcagggttattgtctcatgagcggatacatatttgaatg 3951 tatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaag 4001 tgccacctgacgttcgaggatccggattagtccaatttgttaaagacagg 4051 atatcagtggtccaggctctagttttgactcaacaatatcaccagctgaa 4101 gcctatagagtacgagccatagataaaataaaagattttatttagtctcc 4151 agaaaaaggggggaatgaaagaccccacctgtaggtttggcaagctagct 4201 taagtaacgccattttgcaaggcatggaaaatacataactgagaatagag 4251 aagttcagatcaaggttaggaacagagagacagcagaatatgggccaaac 4301 aggatatctgtggtaagcagttcctgccccgctcagggccaagaacagtt 4351 ggaacaggagaatatgggccaaacaggatatctgtggtaagcagttcctg 4401 ccccggctcagggccaagaacagatggtccccagatgcggtcccgccctc 4451 agcagtttctagagaaccatcagatgtttccagggtgccccaaggacctg 4501 aaatgaccctgtgccttatttgaactaaccaatcagttcgcttctcgctt 4551 ctgttcgcgcgcttctgctccccgagctcaataaaagagcccacaacccc 4601 tcactcggcgcgccagtcctccgatagactgcgtcgcccgggtacccgtg 4651 ttctcaataaaccctcttgcagttgcatccgactcgtggtctcgctgttc 4701 cttgggagggtctcctctgagtgattgactacccgtcagcggggtctttc 4751 agtttctcccacctacacaggtctcactaacattcctgatgtgccgcagg 4801 gactccgtcagcccggtttttgtttataataaaatgcaagaacagtgttc 4851 ccttcaagccagactacatcctgactctcggctttataaaagaatgttga 4901 agggctctgtggactatctgccacacgactttttaagatttttatgcctc 4951 ctggatgagggatttagtcaatctatcctcgtctattttgctggcttctc 5001 cgtattttaaatttctagtttgcactcccttcctgagagcacggcgattg 5051 cagagtagttaatactctgagggcaggcttctgtgaaaaggttgcctggg 5101 ctcagtgtgagattttgccataaaaaggggtcctgcccctgtgtacagac 5151 agatcggaatctagagtgcatactcagagtccccgcggttccggggctct 5201 gatctcagggcatctttgcctagagatcctctacgccggacgcatcgtgg 5251 ccctcgaattaattcgtaatcatggtcatagctgtttcctgtgtgaaatt 5301 gttatccgctcacaattccacacaacatacgagccggaagcataaagtgt 5351 aaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcg 5401 ctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaat 5451 gaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttcc 5501 gcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagc 5551 ggtatcagctcactcaaaggcggtaatacggttatccacagaatcagggg 5601 ataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaac 5651 cgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctga 5701 cgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacag 5751 gactataaagataccaggcgtttccccctggaagctccctcgtgcgctct 5801 cctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttc 5851 gggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcgg 5901 tgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcag 5951 cccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggt 6001 aagacacgacttatcgccactggcagcagccactggtaacaggattagca 6051 gagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaac 6101 tacggctacactagaaggacagtatttggtatctgcgctctgctgaagcc 6151 agttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacca 6201 ccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcaga 6251 aaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgc 6301 tcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaa 6351 aaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatca 6401 atctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaat 6451 cagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttg 6501 cctgactccccgtcgtgtagataactacgatacgggagggcttaccatct 6551 ggccccagtgctgcaatgataccgcgagacccacgctcaccggctccaga 6601 tttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtc 6651 ctgcaactttatccgcctccatccagtctattaattgttgccgggaagct 6701 agagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgc 6751 tacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagct 6801 ccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaa 6851 aaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggc 6901 cgcagtgttatcactcatggttatggcagcactgcataattctcttactg 6951 tcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaag 7001 tcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtc 7051 aatacgggataataccgcgccacatagcagaactttaaaagtgctcatca 7101 ttggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttg 7151 agatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatc 7201 ttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatg 7251 ccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactc 7301 ttcctttttcaatattattgaagcatttatcagggttattgtctcatgag 7351 cggatacatatttgaatgtatttagaaaaataaacaaataggggttccgc 7401 gcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatc 7451 atgacattaacctataaaaataggcgtatcacgaggccctttcgtctcgc 7501 gcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggaga 7551 cggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcag 7601 ggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggc 7651 atcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgc 7701 acagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcagg 7751 ctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacg 7801 ccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgc 7851 cagggttttcccagtcacgacgttgtaaaacgacggccagtgcc JP1520 "Times New Roman% uC{ psi_plus_pack2"Times New Roman uCP>gag"Times New Roman< uCɆMSCV_pri"Times New RomanQ u pLXSN_5_pri"Times New Roman uC pBABE_5_pri"Times New Roman uCɆ< ORF2"Times New Roman uC; K  pBMN_5_pri"Times New Roman3 u| ORF1"Times New Roman uC  puro(12-600)"Times New Roman< uCɆc  CMV_imm_pro"Times New Roman uC  CAG_enhancer"Times New Roman uyCMV_pro"Times New Roman uCɆCMV2_pro"Times New Roman uCLNCX_pri"Times New Roman3 uC pCEP_f_pri"Times New RomanH u .loxP"Times New Roman uCPlAmpR_pro"Times New Roman uC M13_r_pri"Times New Roman uCɆ M13_pUC_r_pri"Times New Roman ulac_pro"Times New Roman uC pBR322_origin"Times New Roman uC$ORF1"Times New Roman< uCɆ$ Ampicillin"Times New Roman uAmpR_pro"Times New Roman uCe{ pGEX_3_pri"Times New Roman uCBlacZ_a"Times New Roman uCɆ M13_pUC_f_pri"Times New Roman u M13_f20_pri"Times New Roman3 uApaI"Times New RomanV  u#BglII(2058), AgeI(2221), PmlI(2383)"Times New Roman9!derr uStuI"Times New Roman '<v uNotI"Times New Roman 7(X`kk uBamHI"Times New RomanB%$$3 utest"Times New Roman><<Qp utest"Times New Roman|  utest"Times New Romang utest"Times New RomanXvh