Sequence of JP1520

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Sequence of JP1520

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Sequence of JP1520 : 7894 BP; 1867 A; 2153 C; 1974 G; 1900 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 aagcttgcat gcctgcaggt cgactctagg cacataaaga aaaacataac taaccaagct 60

1 ttcgaacgta cggacgtcca gctgagatcc gtgtatttct ttttgtattg attggttcga 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gcagccgaga cagtgaaaag aaccgttaaa acggtttgtt ttaaataaac tgaattattt 120

61 cgtcggctct gtcacttttc ttggcaattt tgccaaacaa aatttatttg acttaataaa 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 agagtcattt ctttggtagg aaagtacatt ggcacgtaaa ggagcccaaa gcaatctgtg 180

121 tctcagtaaa gaaaccatcc tttcatgtaa ccgtgcattt cctcgggttt cgttagacac 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaaagcccag gctgggagcc cagcagtttg catcccctcc tggcgtgtac ctaagggttt 240

181 ctttcgggtc cgaccctcgg gtcgtcaaac gtaggggagg accgcacatg gattcccaaa 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cttaattgtg tggtttctaa atcttccaga gggtttgtct cattcacttc cacttcggtg 300

241 gaattaacac accaaagatt tagaaggtct cccaaacaga gtaagtgaag gtgaagccac 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cacaatactt ggacgcggat ttactgtctt agcatctatc ggtggccctt cgattgaggc 360

301 gtgttatgaa cctgcgccta aatgacagaa tcgtagatag ccaccgggaa gctaactccg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tgaacctgag gcccacttct tcagcttgtt aaggagagca caagcaccag aagaggctga 420

361 acttggactc cgggtgaaga agtcgaacaa ttcctctcgt gttcgtggtc ttctccgact 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cccggcagac ctgtgggcat ttttaacaag ggcctcctgg gtctgtggga ggcaggctta 480

421 gggccgtctg gacacccgta aaaattgttc ccggaggacc cagacaccct ccgtccgaat 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cataaggtgc aaattagaaa tataaataat aagcccatat caatttgtca tcttttttta 540

481 gtattccacg tttaatcttt atatttatta ttcgggtata gttaaacagt agaaaaaaat 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 agctcaagtt ttgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt 600

541 tcgagttcaa aactttctgg ggtggacatc caaaccgttc gatcgaattc attgcggtaa 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca 660

601 aacgttccgt accttttatg tattgactct tatctcttca agtctagttc caatccttgt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccgctc 720

661 ctctctgtcg tcttataccc ggtttgtcct atagacacca ttcgtcaagg acggggcgag 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 agggccaaga acagttggaa caggagaata tgggccaaac aggatatctg tggtaagcag 780

721 tcccggttct tgtcaacctt gtcctcttat acccggtttg tcctatagac accattcgtc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca 840

781 aaggacgggg ccgagtcccg gttcttgtct accaggggtc tacgccaggg cgggagtcgt 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc 900

841 caaagatctc ttggtagtct acaaaggtcc cacggggttc ctggacttta ctgggacacg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg 960

901 gaataaactt gattggttag tcaagcgaag agcgaagaca agcgcgcgaa gacgaggggc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt 1020

961 tcgagttatt ttctcgggtg ttggggagtg agccgcgcgg tcaggaggct atctgacgca 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgcccgggta cccgtgttct caataaaccc tcttgcagtt gcatccgact cgtggtctcg 1080

1021 gcgggcccat gggcacaaga gttatttggg agaacgtcaa cgtaggctga gcaccagagc 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg tctttcattt 1140

1081 gacaaggaac cctcccagag gagactcact aactgatggg cagtcgcccc agaaagtaaa 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggaggttcca ccgagatttg gagacccctg cccagggacc accgaccccc ccgccgggag 1200

1141 cctccaaggt ggctctaaac ctctggggac gggtccctgg tggctggggg ggcggccctc 1200

.......... .......... .......... ........ psi_plus_pack2 ........

.......... .......... .......... .......... .......... ..........

1201 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt 1260

1201 cattcgaccg gtcgttgaat agacacagac aggctaacag atcacagata ctgactaaaa 1260

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga 1320

1261 tacgcggacg cagccatgat caatcgattg atcgagacat agaccgcctg ggcaccacct 1320

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga cttcgggggc 1380

1321 tgactgctca agccttgtgg gccggcgttg ggaccctctg cagggtccct gaagcccccg 1380

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgtttttgtg gcccgacctg agtcctaaaa tcccgatcgt ttaggactct ttggtgcacc 1440

1381 gcaaaaacac cgggctggac tcaggatttt agggctagca aatcctgaga aaccacgtgg 1440

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccccttagag gagggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc 1500

1441 ggggaatctc ctccctatac accaagacca tcctctgctc ttggattttg tcaagggcgg 1500

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg 1560

1501 aggcagactt aaaaacgaaa gccaaaccct ggcttcggcg cggcgcgcag aacagacgac 1560

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaatatggg 1620

1561 gtcgtagcaa gacacaacag agacagactg acacaaagac ataaacagac ttttataccc 1620

.......... ........ psi_plus_pack2 ........ .......... ..........

ApaI(1622) .......... .......... .......... .......... ..........

1621 cccgggctag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga 1680

1621 gggcccgatc tgacaatggt gagggaattc aaactggaat ccagtgacct ttctacagct 1680

.......... ...... psi_plus_pack2 gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc 1740

1681 cgcctagcga gtgttggtca gccatctaca gttcttctct gcaacccaat ggaagacgag 1740

.......... ...... psi_plus_pack2 gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct 1800

1741 acgtcttacc ggttggaaat tgcagcctac cggcgctctg ccgtggaaat tggctctgga 1800

.......... ...... psi_plus_pack2 gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt 1860

1801 gtagtgggtc caattctagt tccagaaaag tggaccgggc gtacctgtgg gtctggtcca 1860

.......... ...... psi_plus_pack2 gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt 1920

1861 ggggatgtag cactggaccc ttcggaaccg aaaactgggg ggagggaccc agttcgggaa 1920

.......... ...... psi_plus_pack2 gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc 1980

1921 acatgtggga ttcggaggcg gaggagaagg aggtaggcgg ggcagagagg gggaacttgg 1980

.......... ...... psi_plus_pack2 gag ..... MSCV_pri pLXSN_5_pri

.......... .......... .......... .......... .......... ..........

1981 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc 2040

1981 aggagcaagc tggggcggag ctaggaggga aataggtcgg gagtgaggaa gagatccgcg 2040

MSCV_pri pLXSN_5_pri psi_plus_pack2 gag pBABE_5_pri ..........

.......... .......... .......... .......... .......... ..........

2041 ccccatatgg ccatatgaga tcttatatgg ggcacccccg ccccttgtaa acttccctga 2100

2041 ggggtatacc ggtatactct agaatatacc ccgtgggggc ggggaacatt tgaagggact 2100

.......... ...... psi_plus_pack2 gag ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccctgacatg acaagagtta ctaacagccc ctctctccaa gctcacttac aggctctcta 2160

2101 gggactgtac tgttctcaat gattgtcggg gagagaggtt cgagtgaatg tccgagagat 2160

gag ........ psi_plus_pack2 ORF2 ........ ..........

.......... .......... .......... .......... .......... ..........

2161 cttagtccag cacgaagtct ggagacctct ggcggcagcc taccaagaac aactggaccg 2220

2161 gaatcaggtc gtgcttcaga cctctggaga ccgccgtcgg atggttcttg ttgacctggc 2220

.......... ..... psi_plus_pack2 ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 accggtggta cctcaccctt accgagtcgg cgacacagtg tgggtccgcc gacaccagac 2280

2221 tggccaccat ggagtgggaa tggctcagcc gctgtgtcac acccaggcgg ctgtggtctg 2280

.......... ..... psi_plus_pack2 ORF2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 taagaaccta gaacctcgct ggaaaggacc ttacacagtc ctgctgacca cccccaccgc 2340

2281 attcttggat cttggagcga cctttcctgg aatgtgtcag gacgactggt gggggtggcg 2340

.......... ..... psi_plus_pack2 ORF2 ..... .......... ..........

.......... .......... .......... .......... PmlI(2383) ..........

2341 cctcaaagta gacggcatcg cagcttggat acacgccgcc cacgtgaagg ctgccgaccc 2400

2341 ggagtttcat ctgccgtagc gtcgaaccta tgtgcggcgg gtgcacttcc gacggctggg 2400

.......... ..... psi_plus_pack2 ORF2 pBMN_5_pri ..... ..........

.......... .......... .......... .......... .......... ..........

2401 cgggggtgga ccatcctcta gactgccatg gccgagtaca agcccacggt gcgcctcgcc 2460

2401 gcccccacct ggtaggagat ctgacggtac cggctcatgt tcgggtgcca cgcggagcgg 2460

........ psi_plus_pack2 ORF2 .... ORF1 puro(12-600) ......

.......... .......... .......... .......... .......... ..........

2461 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 2520

2461 tgggcgctgc tgcagggggc ccggcatgcg tgggagcggc ggcgcaagcg gctgatgggg 2520

.......... .... ORF2 ORF1 puro(12-600) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa 2580

2521 cggtgcgcgg tgtggcagct gggcctggcg gtgtagctcg cccagtggct cgacgttctt 2580

.......... .... ORF2 ORF1 puro(12-600) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 2640

2581 gagaaggagt gcgcgcagcc cgagctgtag ccgttccaca cccagcgcct gctgccgcgg 2640

.......... .... ORF2 ORF1 puro(12-600) .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 2700

2641 cgccaccgcc agacctggtg cggcctctcg cagcttcgcc cccgccacaa gcggctctag 2700

.......... .... ORF2 ORF1 puro(12-600) .... .......... ..........

.......... .......... .......... .......... .......... StuI(2759)

2701 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 2760

2701 ccgggcgcgt accggctcaa ctcgccaagg gccgaccggc gcgtcgttgt ctaccttccg 2760

...... ORF2 ORF1 puro(12-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 2820

2761 gaggaccgcg gcgtggccgg gttcctcggg cgcaccaagg accggtggca gccgcagagc 2820

.......... ...... ORF1 puro(12-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 2880

2821 gggctggtgg tcccgttccc agacccgtcg cggcagcacg aggggcctca cctccgccgg 2880

.......... ...... ORF1 puro(12-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 2940

2881 ctcgcgcggc cccacgggcg gaaggacctc tggaggcgcg gggcgttgga ggggaagatg 2940

.......... ...... ORF1 puro(12-600) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gagcggctcg gcttcaccgt caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg 3000

2941 ctcgccgagc cgaagtggca gtggcggctg cagctcacgg gcttcctggc gcgctggacc 3000

.......... ...... ORF1 puro(12-600) ...... .......... ..........

.......... .......... NotI(3030) .......... .......... ..........

3001 tgcatgaccc gcaagcccgg tgcctgacgc ggccgcctcc ggccattagc catattattc 3060

3001 acgtactggg cgttcgggcc acggactgcg ccggcggagg ccggtaatcg gtataataag 3060

...... ORF1 puro(12-600) ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 attggttata tagcataaat caatattggc tattggccat tgcatacgtt gtatccatat 3120

3061 taaccaatat atcgtattta gttataaccg ataaccggta acgtatgcaa cataggtata 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cataatatgt acatttatat tggctcatgt ccaacattac cgccatgttg acattgatta 3180

3121 gtattataca tgtaaatata accgagtaca ggttgtaatg gcggtacaac tgtaactaat 3180

.......... .......... .......... .......... ......... CMV_imm_pro

.......... .......... .......... .......... .......... ..........

3181 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 3240

3181 aactgatcaa taattatcat tagttaatgc cccagtaatc aagtatcggg tatatacctc 3240

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 3300

3241 aaggcgcaat gtattgaatg ccatttaccg ggcggaccga ctggcgggtt gctgggggcg 3300

.......... ........ CMV_imm_pro CAG_enhancer ........ ..........

.......... .......... .......... .......... .......... ..........

3301 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 3360

3301 ggtaactgca gttattactg catacaaggg tatcattgcg gttatccctg aaaggtaact 3360

.......... ... CMV_imm_pro CAG_enhancer ... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 3420

3361 gcagttaccc acctcataaa tgccatttga cgggtgaacc gtcatgtagt tcacatagta 3420

.......... ... CMV_imm_pro CAG_enhancer ... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 3480

3421 tacggttcat gcgggggata actgcagtta ctgccattta ccgggcggac cgtaatacgg 3480

.......... ... CMV_imm_pro CAG_enhancer ... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 3540

3481 gtcatgtact ggaataccct gaaaggatga accgtcatgt agatgcataa tcagtagcga 3540

.......... ... CMV_imm_pro CAG_enhancer ... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 3600

3541 taatggtacc actacgccaa aaccgtcatg tagttacccg cacctatcgc caaactgagt 3600

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 3660

3601 gcccctaaag gttcagaggt ggggtaactg cagttaccct caaacaaaac cgtggtttta 3660

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 3720

3661 gttgccctga aaggttttac agcattgttg aggcggggta actgcgttta cccgccatcc 3720

.......... ......... CMV_imm_pro ......... .......... CMV_pro

.......... .......... .......... .......... .......... ..........

3721 catgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg 3780

3721 gtacatgcca ccctccagat atattcgtct cgagcaaatc acttggcagt ctagcggacc 3780

.......... ..... CMV_imm_pro CMV_pro CMV2_pro LNCX_pri .....

.......... .......... .......... .......... .......... ..........

3781 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgc 3840

3781 tctgcggtag gtgcgacaaa actggaggta tcttctgtgg ccctggctag gtcggaggcg 3840

CMV_pro LNCX_pri pCEP_f_pri CMV2_pro .... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggccccaagc ttataacttc gtatagcata cattatacga agttatagat ccaatattat 3900

3841 ccggggttcg aatattgaag catatcgtat gtaatatgct tcaatatcta ggttataata 3900

.......... .......... loxP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 3960

3901 acttcgtaaa tagtcccaat aacagagtac tcgcctatgt ataaacttac ataaatcttt 3960

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... ......... BamHI(4018)

3961 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgttcgagga 4020

3961 ttatttgttt atccccaagg cgcgtgtaaa ggggcttttc acggtggact gcaagctcct 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tccggattag tccaatttgt taaagacagg atatcagtgg tccaggctct agttttgact 4080

4021 aggcctaatc aggttaaaca atttctgtcc tatagtcacc aggtccgaga tcaaaactga 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 caacaatatc accagctgaa gcctatagag tacgagccat agataaaata aaagatttta 4140

4081 gttgttatag tggtcgactt cggatatctc atgctcggta tctattttat tttctaaaat 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct 4200

4141 aaatcagagg tctttttccc cccttacttt ctggggtgga catccaaacc gttcgatcga 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 taagtaacgc cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat 4260

4201 attcattgcg gtaaaacgtt ccgtaccttt tatgtattga ctcttatctc ttcaagtcta 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 caaggttagg aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag 4320

4261 gttccaatcc ttgtctctct gtcgtcttat acccggtttg tcctatagac accattcgtc 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttcctgcccc gctcagggcc aagaacagtt ggaacaggag aatatgggcc aaacaggata 4380

4321 aaggacgggg cgagtcccgg ttcttgtcaa ccttgtcctc ttatacccgg tttgtcctat 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg 4440

4381 agacaccatt cgtcaaggac ggggccgagt cccggttctt gtctaccagg ggtctacgcc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg 4500

4441 agggcgggag tcgtcaaaga tctcttggta gtctacaaag gtcccacggg gttcctggac 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc 4560

4501 tttactggga cacggaataa acttgattgg ttagtcaagc gaagagcgaa gacaagcgcg 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct 4620

4561 cgaagacgag gggctcgagt tattttctcg ggtgttgggg agtgagccgc gcggtcagga 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccgatagact gcgtcgcccg ggtacccgtg ttctcaataa accctcttgc agttgcatcc 4680

4621 ggctatctga cgcagcgggc ccatgggcac aagagttatt tgggagaacg tcaacgtagg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gactcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc 4740

4681 ctgagcacca gagcgacaag gaaccctccc agaggagact cactaactga tgggcagtcg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ggggtctttc agtttctccc acctacacag gtctcactaa cattcctgat gtgccgcagg 4800

4741 ccccagaaag tcaaagaggg tggatgtgtc cagagtgatt gtaaggacta cacggcgtcc 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gactccgtca gcccggtttt tgtttataat aaaatgcaag aacagtgttc ccttcaagcc 4860

4801 ctgaggcagt cgggccaaaa acaaatatta ttttacgttc ttgtcacaag ggaagttcgg 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agactacatc ctgactctcg gctttataaa agaatgttga agggctctgt ggactatctg 4920

4861 tctgatgtag gactgagagc cgaaatattt tcttacaact tcccgagaca cctgatagac 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ccacacgact ttttaagatt tttatgcctc ctggatgagg gatttagtca atctatcctc 4980

4921 ggtgtgctga aaaattctaa aaatacggag gacctactcc ctaaatcagt tagataggag 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gtctattttg ctggcttctc cgtattttaa atttctagtt tgcactccct tcctgagagc 5040

4981 cagataaaac gaccgaagag gcataaaatt taaagatcaa acgtgaggga aggactctcg 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 acggcgattg cagagtagtt aatactctga gggcaggctt ctgtgaaaag gttgcctggg 5100

5041 tgccgctaac gtctcatcaa ttatgagact cccgtccgaa gacacttttc caacggaccc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctcagtgtga gattttgcca taaaaagggg tcctgcccct gtgtacagac agatcggaat 5160

5101 gagtcacact ctaaaacggt atttttcccc aggacgggga cacatgtctg tctagcctta 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctagagtgca tactcagagt ccccgcggtt ccggggctct gatctcaggg catctttgcc 5220

5161 gatctcacgt atgagtctca ggggcgccaa ggccccgaga ctagagtccc gtagaaacgg 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tagagatcct ctacgccgga cgcatcgtgg ccctcgaatt aattcgtaat catggtcata 5280

5221 atctctagga gatgcggcct gcgtagcacc gggagcttaa ttaagcatta gtaccagtat 5280

.......... .......... .......... .......... .......... M13_r_pri

.......... .......... .......... .......... .......... ..........

5281 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 5340

5281 cgacaaagga cacactttaa caataggcga gtgttaaggt gtgttgtatg ctcggccttc 5340

M13_r_pri M13_pUC_r_pri ...... .......... .......... lac_pro

.......... .......... .......... .......... .......... ..........

5341 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 5400

5341 gtatttcaca tttcggaccc cacggattac tcactcgatt gagtgtaatt aacgcaacgc 5400

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 5460

5401 gagtgacggg cgaaaggtca gccctttgga cagcacggtc gacgtaatta cttagccggt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 5520

5461 tgcgcgcccc tctccgccaa acgcataacc cgcgagaagg cgaaggagcg agtgactgag 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 5580

5521 cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc gccattatgc 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 5640

5581 caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc cggtcgtttt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 5700

5641 ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg cggggggact 5700

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5701 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 5760

5701 gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc ctgatatttc 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 5820

5761 tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct gggacggcga 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 5880

5821 atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag tatcgagtgc 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 5940

5881 gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac acgtgcttgg 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 6000

5941 ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca ggttgggcca 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 6060

6001 ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 6120

6061 acatccgcca cgatgtctca agaacttcac caccggattg atgccgatgt gatcttcctg 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 6180

6121 tcataaacca tagacgcgag acgacttcgg tcaatggaag cctttttctc aaccatcgag 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 6240

6181 aactaggccg tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 6300

6241 atgcgcgtct ttttttccta gagttcttct aggaaactag aaaagatgcc ccagactgcg 6300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6301 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 6360

6301 agtcaccttg cttttgagtg caattcccta aaaccagtac tctaatagtt tttcctagaa 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 6420

6361 gtggatctag gaaaatttaa tttttacttc aaaatttagt tagatttcat atatactcat 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 6480

6421 ttgaaccaga ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc gctagacaga 6480

.......... .......... ....... ORF1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

6481 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 6540

6481 taaagcaagt aggtatcaac ggactgaggg gcagcacatc tattgatgct atgccctccc 6540

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 6600

6541 gaatggtaga ccggggtcac gacgttacta tggcgctctg ggtgcgagtg gccgaggtct 6600

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 6660

6601 aaatagtcgt tatttggtcg gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa 6660

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 6720

6661 taggcggagg taggtcagat aattaacaac ggcccttcga tctcattcat caagcggtca 6720

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 6780

6721 attatcaaac gcgttgcaac aacggtaacg atgtccgtag caccacagtg cgagcagcaa 6780

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 6840

6781 accataccga agtaagtcga ggccaagggt tgctagttcc gctcaatgta ctagggggta 6840

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 6900

6841 caacacgttt tttcgccaat cgaggaagcc aggaggctag caacagtctt cattcaaccg 6900

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 6960

6901 gcgtcacaat agtgagtacc aataccgtcg tgacgtatta agagaatgac agtacggtag 6960

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 7020

6961 gcattctacg aaaagacact gaccactcat gagttggttc agtaagactc ttatcacata 7020

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 7080

7021 cgccgctggc tcaacgagaa cgggccgcag ttatgcccta ttatggcgcg gtgtatcgtc 7080

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 7140

7081 ttgaaatttt cacgagtagt aaccttttgc aagaagcccc gcttttgaga gttcctagaa 7140

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 7200

7141 tggcgacaac tctaggtcaa gctacattgg gtgagcacgt gggttgacta gaagtcgtag 7200

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 7260

7201 aaaatgaaag tggtcgcaaa gacccactcg tttttgtcct tccgttttac ggcgtttttt 7260

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 7320

7261 cccttattcc cgctgtgcct ttacaactta tgagtatgag aaggaaaaag ttataataac 7320

....... ORF1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 7380

7321 ttcgtaaata gtcccaataa cagagtactc gcctatgtat aaacttacat aaatcttttt 7380

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

7381 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 7440

7381 atttgtttat ccccaaggcg cgtgtaaagg ggcttttcac ggtggactgc agattctttg 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 7500

7441 gtaataatag tactgtaatt ggatattttt atccgcatag tgctccggga aagcagagcg 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 7560

7501 cgcaaagcca ctactgccac ttttggagac tgtgtacgtc gagggcctct gccagtgtcg 7560

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

7561 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 7620

7561 aacagacatt cgcctacggc cctcgtctgt tcgggcagtc ccgcgcagtc gcccacaacc 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 7680

7621 gcccacagcc ccgaccgaat tgatacgccg tagtctcgtc taacatgact ctcacgtggt 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 7740

7681 atacgccaca ctttatggcg tgtctacgca ttcctctttt atggcgtagt ccgcggtaag 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 7800

7741 cggtaagtcc gacgcgttga caacccttcc cgctagccac gcccggagaa gcgataatgc 7800

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 7860

7801 ggtcgaccgc tttcccccta cacgacgttc cgctaattca acccattgcg gtcccaaaag 7860

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ccagtcacga cgttgtaaaa cgacggccag tgcc...... .......... .......... 7894

7861 ggtcagtgct gcaacatttt gctgccggtc acgg...... .......... .......... 7894

.... lacZ_a M13_pUC_f_pri M13_f20_pri .... .......... ..........