Sequence of L071

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Sequence of L071

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Sequence of L071: 8254 BP; 1867 A; 2325 C; 2166 G; 1896 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cctagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 60

1 ggatcaataa ttatcattag ttaatgcccc agtaatcaag tatcgggtat atacctcaag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 120

61 gcgcaatgta ttgaatgcca tttaccgggc ggaccgactg gcgggttgct gggggcgggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 180

121 aactgcagtt attactgcat acaagggtat cattgcggtt atccctgaaa ggtaactgca 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 240

181 gttacccacc tcataaatgc catttgacgg gtgaaccgtc atgtagttca catagtatac 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 300

241 ggttcatgcg ggggataact gcagttactg ccatttaccg ggcggaccgt aatacgggtc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 360

301 atgtactgga ataccctgaa aggatgaacc gtcatgtaga tgcataatca gtagcgataa 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg 420

361 tggtaccact acgccaaaac cgtcatgtag ttacccgcac ctatcgccaa actgagtgcc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 480

421 cctaaaggtt cagaggtggg gtaactgcag ttaccctcaa acaaaaccgt ggttttagtt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcat 540

481 gccctgaaag gttttacagc attgttgagg cggggtaact gcgtttaccc gccatccgta 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gtacggtggg aggtctatat aagcagagct caataaaaga gcccacaacc cctcactcgg 600

541 catgccaccc tccagatata ttcgtctcga gttattttct cgggtgttgg ggagtgagcc 600

.......... .......... .......... .......... .......... ..........

AscI(601) .......... XmaI(630),SmaI(632) KpnI(638) ..........

601 cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt 660

601 gcgcggtcag gaggctatct gacgcagcgg gcccatgggc ataagggtta tttcggagaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc agattgattg 720

661 cgacaaacgt aggcttagca cctgagcgac taggaaccct cccagaggag tctaactaac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag acccctgccc 780

721 tgacgggtgg agcccccaga aagtaaacct ccaaggtggc tctaaacctc tggggacggg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc gtgtctgtct 840

781 tccctggtgg ctgggggggc ggccctccat tcgaccggtc gccagcaaag cacagacaga 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg tactagttag 900

841 gacagaaaca cgcacaaaca cggccgtaga ttacaaacgc ggacgcagac atgatcaatc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc 960

901 gattgatcga gacatagacc gcctgggcac caccttgact gctcaagcct tgtgggccgg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc 1020

961 cgttgggacc ctctgcaggg tccctgaagc ccccggcaaa aacaccgggc tggactcagg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aaaaatcccg atcgttttgg actctttggt gcacccccct tagaggaggg atatgtggtt 1080

1021 tttttagggc tagcaaaacc tgagaaacca cgtgggggga atctcctccc tatacaccaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt 1140

1081 gaccatcctc tgctcttgga ttttgtcaag ggcggaggca gacttaaaaa cgaaagccaa 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 1200

1141 accctggctt cggcgcggcg cgcagaacag acgacgtcgt agcaagacac aacagagaca 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gccagactgt taccactccc 1260

1201 gactgacaca aagacataaa cagactttta tacccgggcc cggtctgaca atggtgaggg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta 1320

1261 aattcaaact ggaatccagt gacctttcta cagctcgcct agcgagtgtt ggtcagccat 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... PstI(1360) .......... ..........

1321 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc 1380

1321 ctacagttct tctctgcaac ccaatggaag acgagacgtc ttaccggttg gaaattgcag 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc 1440

1381 cctaccggcg ctctgccgtg gaaattggct ctggagtagt gggtccaatt ctagttccag 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc 1500

1441 aaaagtggac cgggcgtacc tgtgggtctg gtccagggga tgtagcactg gacccttcgg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct 1560

1501 aaccgaaaac tggggggagg gacccagttc gggaaacatg tgggattcgg aggcggagga 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc 1620

1561 gaaggaggta ggcggggcag agagggggaa cttggaggag caagctgggg cggagctagg 1620

.......... .......... .. pLXSN_5_primer MSCV_primer .. ..........

.......... .......... .......... .... NdeI(1661) NdeI(1669) ....

1621 tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata tgagatcgat 1680

1621 agggaaatag gtcgggagtg aggaagagat ccgcgggggt ataccggtat actctagcta 1680

........ pBABE_5_primer ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac 1740

1681 gaatataccc cgtgggggcg gggaacattt gaagggactg ggactgtact gttctcaatg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg 1800

1741 attgtcgggg agagaggttc gagtgaatgt ccgagagatg aatcaggtcg tgcttcagac 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta 1860

1801 ctctggagac cgccgtcgga tggttcttgt tgacctggct ggccaccatg gagtgggaat 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg 1920

1861 ggctcagccg ctgtgtcaca cccaggcggc tgtggtctga ttcttggatc ttggagcgac 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc 1980

1921 ctttcctgga atgtgtcagg acgactggtg ggggtggcgg gagtttcatc tgccgtagcg 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XmaI(2019) SmaI(2021) ..........

1981 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag 2040

1981 tcgaacctat gtgcggcggg tgcacttccg acggctgggg cccccacctg gtaggagatc 2040

pBMN_5_primer ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 actgccggat ctcgaaggat cttccccacc atgcctgcta ttgtcttccc aatcctcccc 2100

2041 tgacggccta gagcttccta gaaggggtgg tacggacgat aacagaaggg ttaggagggg 2100

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

2101 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga 2160

2101 gaacgacagg acggggtggg gtggggggtc ttatcttact gtggatgagt ctgttacgct 2160

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga 2220

2161 acgttaaagg agtaaaataa tcctttcctg tcaccctcac cgtggaaggt cccagttcct 2220

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggtcta 2280

2221 tccgtgcccc ctccccgttt gttgtctacc gaccgttgat cttccgtgtc agctccagat 2280

.......... .......... ORF 1 . BGH_rev_primer BGHrev_primer .

NotI(2283),EagI(2283) XbaI(2290) ........ .......... ..........

2281 gcggccgctc tagatcaggc accgggcttg cgggtcatgc accaggtcgc gcggtccttc 2340

2281 cgccggcgag atctagtccg tggcccgaac gcccagtacg tggtccagcg cgccaggaag 2340

.......... .......... ORF 1 ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg 2400

2341 cccgtggagc tgcagccgcc actgccactt cggctcggcg agcatcttcc cctccaacgc 2400

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... EagI(2443) ..........

2401 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg 2460

2401 cccgcgcctc cagaggtcct tccgcccgtg gggccgcgcg agccggcgga ggtgaggccc 2460

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc 2520

2461 ctcgtgctgc cgcgacgggt ctgggaacgg gaccaccagc ccgctctgcg gctgccaccg 2520

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg 2580

2521 gtccttggtg cgcccgagga acccggccac gccgcggtcc tccggaaggt agacaacgac 2580

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgcggccagc cgggaaccgc tcaactcggc catgcgcgga ccgatctcgg cgaacaccgc 2640

2581 gcgccggtcg gcccttggcg agttgagccg gtacgcgcct ggctagagcc gcttgtggcg 2640

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac 2700

2641 ggggcgaagc tgcgagaggc cgcaccaggt ctggcggtgg cgccgcggca gcaggcgctg 2700

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac 2760

2701 ggtgtggaac ggctacagct cgggctgcgc gcactccttc tcaagaacgt cgagccactg 2760

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccgctcgatg tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt cggcgaacgc 2820

2761 ggcgagctac accgccaggc ccagctgcca caccgcgcac cgccccatca gccgcttgcg 2820

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggcggcgagg gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc gcaccgtggg 2880

2821 ccgccgctcc cacgcatgcc gggccccctg cagcagcgcc caccgctccg cgtggcaccc 2880

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cttgtactct gtcattgatg tggtgggccc agggttggac tcgacgtctc ccgcaagttt 2940

2881 gaacatgaga cagtaactac accacccggg tcccaacctg agctgcagag ggcgttcaaa 2940

ORF 1 .......... ORF 2 .......... .......... ..........

.......... ......... PvuII(2962) ........ EcoRI(2987) .........

2941 aagaaggtca aaattcaaca gctgaccgcc accgctgcca cctccgaatt ccttgtacag 3000

2941 ttcttccagt tttaagttgt cgactggcgg tggcgacggt ggaggcttaa ggaacatgtc 3000

.......... .......... ORF 2 .......... .......... EGFP

.......... .......... .......... .......... .......... ..........

3001 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc 3060

3001 gagcaggtac ggctctcact agggccgccg ccagtgcttg aggtcgtcct ggtacactag 3060

.......... .......... ORF 2 EGFP ........ EGFP_C_primer ........

.......... .......... .......... .......... .......... ..........

3061 gcgcttctcg ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg 3120

3061 cgcgaagagc aaccccagaa acgagtcccg cctgacccac gagtccatca ccaacagccc 3120

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac 3180

3121 gtcgtcgtgc cccggcagcg gctaccccca caagacgacc atcaccagcc gctcgacgtg 3180

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt 3240

3181 cgacggcagg agctacaaca ccgcctagaa cttcaagtgg aactacggca agaagacgaa 3240

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat 3300

3241 cagccggtac tatatctgca acaccgacaa catcaacatg aggtcgaaca cggggtccta 3300

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc 3360

3301 caacggcagg aggaacttca gctacgggaa gtcgagctac gccaagtggt cccacagcgg 3360

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg 3420

3361 gagcttgaag tggagccgcg cccagaacat caacggcagc aggaacttct tctaccacgc 3420

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc 3480

3421 gaggacctgc atcggaagcc cgtaccgcct gaacttcttc agcacgacga agtacaccag 3480

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg 3540

3481 ccccatcgcc gacttcgtga cgtgcggcat ccagtcccac cagtgctccc acccggtccc 3540

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc 3600

3541 gtgcccgtcg aacggccacc acgtctactt gaagtcccag tcgaacggca tccaccgtag 3600

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac 3660

3601 cgggagcggg agcggcctgt gcgacttgaa caccggcaaa tgcagcggca ggtcgagctg 3660

.......... .......... ORF 2 EGFP .......... ........ EGFP_N_primer

.......... .......... .......... .......... NcoI(3706) ClaI(3716)

3661 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggatcgatc 3720

3661 gtcctacccg tggtggggcc acttgtcgag gagcgggaac gagtggtacc acctagctag 3720

.......... .......... ORF 2 EGFP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctaggccacg tgttcacgac acctgaaatg gaagaaaaaa actttgaacc actgtctgag 3780

3721 gatccggtgc acaagtgctg tggactttac cttctttttt tgaaacttgg tgacagactc 3780

.......... .......... .......... .......... ....... EF1a_fwd_primer

.......... .......... .......... .......... .......... ..........

3781 gcttgagaat gaaccaagat ccaaactcaa aaaggccaaa ttccaaggag aattacatca 3840

3781 cgaactctta cttggttcta ggtttgagtt tttccggttt aaggttcctc ttaatgtagt 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 agtgccaagc tggcctaact tcagtctcca cccactcagt gtggggaaac tccatcgcat 3900

3841 tcacggttcg accggattga agtcagaggt gggtgagtca cacccctttg aggtagcgta 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 aaaacccctc cccccaacct aaagacgacg tactccaaaa gctccagaac taatcgaggt 3960

3901 ttttggggag gggggttgga tttctgctgc atgaggtttt cgaggtcttg attagctcca 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcctggacgg cgcccggtac tccgtggagt cacatgaagc gacggctgag gacggaaagg 4020

3961 cggacctgcc gcgggccatg aggcacctca gtgtacttcg ctgccgactc ctgcctttcc 4020

.......... .......... .......... .......... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

4021 cccctttcct ttgtgtgggt gactcacccg cccgctctcc cgagcgccgc gtcctccatt 4080

4021 ggggaaagga aacacaccca ctgagtgggc gggcgagagg gctcgcggcg caggaggtaa 4080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttgagccccc tggagcaggg ccgggaagcg gccatctttc cgctcacgca actggtgccg 4140

4081 aactcggggg acctcgtccc ggcccttcgc cggtagaaag gcgagtgcgt tgaccacggc 4140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 accgggccag ccttgccgcc cagggcgggg cgatacacgg cggcgcgagg ccaggcacca 4200

4141 tggcccggtc ggaacggcgg gtcccgcccc gctatgtgcc gccgcgctcc ggtccgtggt 4200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gagcaggccg gccagcttga gactaccccc gtccgattct cggtggccgc gctcgcaggc 4260

4201 ctcgtccggc cggtcgaact ctgatggggg caggctaaga gccaccggcg cgagcgtccg 4260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... ApaI(4299) .......... ..........

4261 cccgcctcgc cgaacatgtg cgctgggacg cacgggcccc gtcgccgccc gcggccccaa 4320

4261 gggcggagcg gcttgtacac gcgaccctgc gtgcccgggg cagcggcggg cgccggggtt 4320

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aaaccgaaat accagtgtgc agatcctggc ccgcatttac aagactatct tgccagaaaa 4380

4321 tttggcttta tggtcacacg tctaggaccg ggcgtaaatg ttctgataga acggtctttt 4380

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aaagcgtcgc agcaggtcat caaaaatttt aaatggctag agacttatcg aaagcagcga 4440

4381 tttcgcagcg tcgtccagta gtttttaaaa tttaccgatc tctgaatagc tttcgtcgct 4440

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gacaggcgcg aaggtgccac cagattcgca cgcggcggcc ccagcgccca ggccaggcct 4500

4441 ctgtccgcgc ttccacggtg gtctaagcgt gcgccgccgg ggtcgcgggt ccggtccgga 4500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 caactcaagc acgaggcgaa ggggctccta aagcgcaagg cccgcccctg gctccagctc 4560

4501 gttgagttcg tgctccgctt ccccgaggat ttcgcgttcc gggcggggac cgaggtcgag 4560

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gggatcaaga atcacgtact ggagccaggt ggaagtaatt caaggcacgc aagggccata 4620

4561 ccctagttct tagtgcatga cctcggtcca ccttcattaa gttccgtgcg ttcccggtat 4620

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acccgtaaag aggccaggcc cgcgggaacc acacacggca cttacctgtg ttctggcggc 4680

4621 tgggcatttc tccggtccgg gcgcccttgg tgtgtgccgt gaatggacac aagaccgccg 4680

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aaacccgttg cgaaaaagaa cgttcacggc gactactgca cttatatacg gttctccccc 4740

4681 tttgggcaac gctttttctt gcaagtgccg ctgatgacgt gaatatatgc caagaggggg 4740

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 accctcggga aaaaggcgga gccagtacac gacatcactt tcccagttta ccccgcgcca 4800

4741 tgggagccct ttttccgcct cggtcatgtg ctgtagtgaa agggtcaaat ggggcgcggt 4800

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccttctctag gcaccggttc aattgccgac ccctcccccc aacttctcgg ggactgtggg 4860

4801 ggaagagatc cgtggccaag ttaacggctg gggagggggg ttgaagagcc cctgacaccc 4860

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgatgtgcgc tctgcccact gacgggcacc ggagcctcac gctcgagagg ccgctcgacg 4920

4861 gctacacgcg agacgggtga ctgcccgtgg cctcggagtg cgagctctcc ggcgagctgc 4920

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt 4980

4921 tattttattt tctaaaataa atcagaggtc tttttccccc cttactttct ggggtggaca 4980

ORF 2 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg 5040

4981 tccaaaccgt tcgatcgaat tcattgcggt aaaacgttcc gtaccttttt atgtattgac 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 agaatagaaa agttcagatc aaggtcagga acagaggaac aggtcgacca aggtcgggca 5100

5041 tcttatcttt tcaagtctag ttccagtcct tgtctccttg tccagctggt tccagcccgt 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ggaagagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag 5160

5101 ccttctcccg gataaagggt actaaggaag tataaacgta tatgctatgt tccgacaatc 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agagataatt agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg 5220

5161 tctctattaa tcttaattaa actgacattt gtgtttctat aatcatgttt tatgcactgc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta 5280

5221 atctttcatt attaaagaac ccatcaaacg tcaaaatttt aatacaaaat tttacctgat 5280

.......... .......... .......... .......... .......... ..........

NdeI(5284) .......... .......... .......... .......... ..........

5281 tcatatgctt accgtaactt gaaagtactc tatcattgat agagttatat atcttgtgga 5340

5281 agtatacgaa tggcattgaa ctttcatgag atagtaacta tctcaatata tagaacacct 5340

LKO1_5_primer ...... .......... .......... .......... ..........

BglII(5348) ....... BamHI(5362) ......... .......... ..........

5341 aaggacagat ctccgcatgc ggatccagga acaatggcag ctttcttgcc atcaaagctg 5400

5341 ttcctgtcta gaggcgtacg cctaggtcct tgttaccgtc gaaagaacgg tagtttcgac 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ctgatggcac atatattctt aatggtgact acactttgtc caccttagag caagacatta 5460

5401 gactaccgtg tatataagaa ttaccactga tgtgaaacag gtggaatctc gttctgtaat 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tgtacaaagg tgttgtcttg aggtacagcg gctcctctgc ggcattggaa agaattcgca 5520

5461 acatgtttcc acaacagaac tccatgtcgc cgaggagacg ccgtaacctt tcttaagcgt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gctttagccc tctcaaagag cccttgacca tccaggttct tactgtgggc aatgcccttc 5580

5521 cgaaatcggg agagtttctc gggaactggt aggtccaaga atgacacccg ttacgggaag 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gacctaaaat taaatacacc tacttcgtaa agaagaagaa ggaatctttc aatgctatcc 5640

5581 ctggatttta atttatgtgg atgaagcatt tcttcttctt ccttagaaag ttacgatagg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ccactttttc agcatgggtc attgaagagt ggggcgaatg ttctaagtca tgtgaattgg 5700

5641 ggtgaaaaag tcgtacccag taacttctca ccccgcttac aagattcagt acacttaacc 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gttggcagag aagactggta gaatgccgag acatcacctg catgcttttt ccatactttt 5760

5701 caaccgtctc ttctgaccat cttacggctc tgtagtggac gtacgaaaaa ggtatgaaaa 5760

.......... .......... .......... .......... .......... ..........

HindIII(5768) ...... .......... .......... .......... ..........

5761 tttggcaagc ttacgcgtga gcccacaacc cctcactcgg ggcgccagtc ctccgattga 5820

5761 aaaccgttcg aatgcgcact cgggtgttgg ggagtgagcc ccgcggtcag gaggctaact 5820

.......... .......... .......... .......... .......... ..........

XmaI(5830),SmaI(5832) KpnI(5838) ........ .......... ..........

5821 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg 5880

5821 gactcagcgg gcccatgggc acataggtta tttgggagaa cgtcaacgta ggctgaacac 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct 5940

5881 cagagcgaca aggaaccctc ccagaggaga ctcactaact gatgggcagt cgcccccaga 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt tacattaaat 6000

5941 aagtgtacgt cgtacatagt tttaattaaa ccaaaaaaaa gaattcataa atgtaattta 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ggccatagta gttcattatg gacagcgcag aaagagctgg ggagaattgt gaaattgtta 6060

6001 ccggtatcat caagtaatac ctgtcgcgtc tttctcgacc cctcttaaca ctttaacaat 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6120

6061 aggcgagtgt taaggtgtgt tgtatgctcg gccttcgtat ttcacatttc ggaccccacg 6120

.......... .......... ......... lac_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

6121 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6180

6121 gattactcac tcgattgagt gtaattaacg caacgcgagt gacgggcgaa aggtcagccc 6180

.......... .......... .......... .......... .......... ..........

......... PvuII(6197) ......... .......... .......... ..........

6181 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 6240

6181 tttggacagc acggtcgacg taattactta gccggttgcg cgcccctctc cgccaaacgc 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 6300

6241 ataacccgcg agaaggcgaa ggagcgagtg actgagcgac gcgagccagc aagccgacgc 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 6360

6301 cgctcgccat agtcgagtga gtttccgcca ttatgccaat aggtgtctta gtcccctatt 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 6420

6361 gcgtcctttc ttgtacactc gttttccggt cgttttccgg tccttggcat ttttccggcg 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 6480

6421 caacgaccgc aaaaaggtat ccgaggcggg gggactgctc gtagtgtttt tagctgcgag 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 6540

6481 ttcagtctcc accgctttgg gctgtcctga tatttctatg gtccgcaaag ggggaccttc 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 6600

6541 gagggagcac gcgagaggac aaggctggga cggcgaatgg cctatggaca ggcggaaaga 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 6660

6601 gggaagccct tcgcaccgcg aaagagtatc gagtgcgaca tccatagagt caagccacat 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 6720

6661 ccagcaagcg aggttcgacc cgacacacgt gcttgggggg caagtcgggc tggcgacgcg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 6780

6721 gaataggcca ttgatagcag aactcaggtt gggccattct gtgctgaata gcggtgaccg 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 6840

6781 tcgtcggtga ccattgtcct aatcgtctcg ctccatacat ccgccacgat gtctcaagaa 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 6900

6841 cttcaccacc ggattgatgc cgatgtgatc ttcctgtcat aaaccataga cgcgagacga 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 6960

6901 cttcggtcaa tggaagcctt tttctcaacc atcgagaact aggccgtttg tttggtggcg 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 7020

6961 accatcgcca ccaaaaaaac aaacgttcgt cgtctaatgc gcgtcttttt ttcctagagt 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 7080

7021 tcttctagga aactagaaaa gatgccccag actgcgagtc accttgcttt tgagtgcaat 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7140

7081 tccctaaaac cagtactcta atagtttttc ctagaagtgg atctaggaaa atttaatttt 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 atgaagtttg cgcaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 7200

7141 tacttcaaac gcgtttagtt agatttcata tatactcatt tgaaccagac tgtcaatggt 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 7260

7201 tacgaattag tcactccgtg gatagagtcg ctagacagat aaagcaagta ggtatcaacg 7260

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 7320

7261 gactgagggg cagcacatct attgatgcta tgccctcccg aatggtagac cggggtcacg 7320

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 7380

7321 acgttactat ggcgctctgg gtgcgagtgg ccgaggtcta aatagtcgtt atttggtcgg 7380

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 7440

7381 tcggccttcc cggctcgcgt cttcaccagg acgttgaaat aggcggaggt aggtcagata 7440

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 7500

7441 attaacaacg gcccttcgat ctcattcatc aagcggtcaa ttatcaaacg cgttgcaaca 7500

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 7560

7501 acggtaacga tgtccgtagc accacagtgc gagcagcaaa ccataccgaa gtaagtcgag 7560

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 7620

7561 gccaagggtt gctagttccg ctcaatgtac tagggggtac aacacgtttt ttcgccaatc 7620

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 7680

7621 gaggaagcca ggaggctagc aacagtcttc attcaaccgg cgtcacaata gtgagtacca 7680

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 7740

7681 ataccgtcgt gacgtattaa gagaatgaca gtacggtagg cattctacga aaagacactg 7740

.......... .......... bla ORF 1 .......... .......... ..........

ScaI(7749) .......... .......... .......... .......... ..........

7741 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 7800

7741 accactcatg agttggttca gtaagactct tatcacatac gccgctggct caacgagaac 7800

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 7860

7801 gggccgcagt tgtgccctat tatggcgcgg tgtatcgtct tgaaattttc acgagtagta 7860

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 7920

7861 accttttgca agaagccccg cttttgagag ttcctagaat ggcgacaact ctaggtcaag 7920

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 7980

7921 ctacattggg tgagcacgtg ggttgactag aagtcgtaga aaatgaaagt ggtcgcaaag 7980

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 8040

7981 acccactcgt ttttgtcctt ccgttttacg gcgttttttc ccttattccc gctgtgcctt 8040

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 8100

8041 tacaacttat gagtatgaga aggaaaaagt tataataact tcgtaaatag tcccaataac 8100

bla ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 8160

8101 agagtactcg cctatgtata aacttacata aatcttttta tttgtttatc cccaaggcgc 8160

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 cacatttccc cgaaaagtgc caccagcttt gctcttagga gtttcctaat acatcccaaa 8220

8161 gtgtaaaggg gcttttcacg gtggtcgaaa cgagaatcct caaaggatta tgtagggttt 8220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 ctcaaatata taaagcattt gacttgttct atgc...... .......... .......... 8254

8221 gagtttatat atttcgtaaa ctgaacaaga tacg...... .......... .......... 8254

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-8254 Reference author: Micklem D.R., Lorens J.B.; Reference title: "Retroviral vectors for expression of shRNAs"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-8254 Reference author: Micklem D.R., Lorens J.B.; Reference title: ; Reference location: Submitted (24-JAN-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Biomedicine, University of Bergen, Jonas Lies vei 91, Bergen 5009, Norway