Sequence of L087

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

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Sequence of L087

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Sequence of L087: 8215 BP; 1858 A; 2332 C; 2154 G; 1871 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cctagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc 60

1 ggatcaataa ttatcattag ttaatgcccc agtaatcaag tatcgggtat atacctcaag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca 120

61 gcgcaatgta ttgaatgcca tttaccgggc ggaccgactg gcgggttgct gggggcgggt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt 180

121 aactgcagtt attactgcat acaagggtat cattgcggtt atccctgaaa ggtaactgca 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg 240

181 gttacccacc tcataaatgc catttgacgg gtgaaccgtc atgtagttca catagtatac 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag 300

241 ggttcatgcg ggggataact gcagttactg ccatttaccg ggcggaccgt aatacgggtc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt 360

301 atgtactgga ataccctgaa aggatgaacc gtcatgtaga tgcataatca gtagcgataa 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg 420

361 tggtaccact acgccaaaac cgtcatgtag ttacccgcac ctatcgccaa actgagtgcc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa 480

421 cctaaaggtt cagaggtggg gtaactgcag ttaccctcaa acaaaaccgt ggttttagtt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcat 540

481 gccctgaaag gttttacagc attgttgagg cggggtaact gcgtttaccc gccatccgta 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gtacggtggg aggtctatat aagcagagct caataaaaga gcccacaacc cctcactcgg 600

541 catgccaccc tccagatata ttcgtctcga gttattttct cgggtgttgg ggagtgagcc 600

.......... .......... .......... .......... .......... ..........

AscI(601) .......... XmaI(630),SmaI(632) KpnI(638) ..........

601 cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt 660

601 gcgcggtcag gaggctatct gacgcagcgg gcccatgggc ataagggtta tttcggagaa 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 gctgtttgca tccgaatcgt ggactcgctg atccttggga gggtctcctc agattgattg 720

661 cgacaaacgt aggcttagca cctgagcgac taggaaccct cccagaggag tctaactaac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 actgcccacc tcgggggtct ttcatttgga ggttccaccg agatttggag acccctgccc 780

721 tgacgggtgg agcccccaga aagtaaacct ccaaggtggc tctaaacctc tggggacggg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agggaccacc gacccccccg ccgggaggta agctggccag cggtcgtttc gtgtctgtct 840

781 tccctggtgg ctgggggggc ggccctccat tcgaccggtc gccagcaaag cacagacaga 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctgtctttgt gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg tactagttag 900

841 gacagaaaca cgcacaaaca cggccgtaga ttacaaacgc ggacgcagac atgatcaatc 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc 960

901 gattgatcga gacatagacc gcctgggcac caccttgact gctcaagcct tgtgggccgg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc 1020

961 cgttgggacc ctctgcaggg tccctgaagc ccccggcaaa aacaccgggc tggactcagg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aaaaatcccg atcgttttgg actctttggt gcacccccct tagaggaggg atatgtggtt 1080

1021 tttttagggc tagcaaaacc tgagaaacca cgtgggggga atctcctccc tatacaccaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt 1140

1081 gaccatcctc tgctcttgga ttttgtcaag ggcggaggca gacttaaaaa cgaaagccaa 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt 1200

1141 accctggctt cggcgcggcg cgcagaacag acgacgtcgt agcaagacac aacagagaca 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gccagactgt taccactccc 1260

1201 gactgacaca aagacataaa cagactttta tacccgggcc cggtctgaca atggtgaggg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta 1320

1261 aattcaaact ggaatccagt gacctttcta cagctcgcct agcgagtgtt ggtcagccat 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... PstI(1360) .......... ..........

1321 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc 1380

1321 ctacagttct tctctgcaac ccaatggaag acgagacgtc ttaccggttg gaaattgcag 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc 1440

1381 cctaccggcg ctctgccgtg gaaattggct ctggagtagt gggtccaatt ctagttccag 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc 1500

1441 aaaagtggac cgggcgtacc tgtgggtctg gtccagggga tgtagcactg gacccttcgg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct 1560

1501 aaccgaaaac tggggggagg gacccagttc gggaaacatg tgggattcgg aggcggagga 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc 1620

1561 gaaggaggta ggcggggcag agagggggaa cttggaggag caagctgggg cggagctagg 1620

.......... .......... .. pLXSN_5_primer MSCV_primer .. ..........

.......... .......... .......... .... NdeI(1661) NdeI(1669) ....

1621 tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata tgagatcgat 1680

1621 agggaaatag gtcgggagtg aggaagagat ccgcgggggt ataccggtat actctagcta 1680

........ pBABE_5_primer ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac 1740

1681 gaatataccc cgtgggggcg gggaacattt gaagggactg ggactgtact gttctcaatg 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg 1800

1741 attgtcgggg agagaggttc gagtgaatgt ccgagagatg aatcaggtcg tgcttcagac 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... KpnI(1851)

1801 gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta 1860

1801 ctctggagac cgccgtcgga tggttcttgt tgacctggct ggccaccatg gagtgggaat 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg 1920

1861 ggctcagccg ctgtgtcaca cccaggcggc tgtggtctga ttcttggatc ttggagcgac 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc 1980

1921 ctttcctgga atgtgtcagg acgactggtg ggggtggcgg gagtttcatc tgccgtagcg 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .... XmaI(2019),SmaI(2021) ....

1981 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag 2040

1981 tcgaacctat gtgcggcggg tgcacttccg acggctgggg cccccacctg gtaggagatc 2040

pBMN_5_primer ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 actgccggat ctcgaaggat cttccccacc atgcctgcta ttgtcttccc aatcctcccc 2100

2041 tgacggccta gagcttccta gaaggggtgg tacggacgat aacagaaggg ttaggagggg 2100

.......... .......... .......... .......... ORF 1 ..........

.......... .......... .......... .......... .......... ..........

2101 cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca gacaatgcga 2160

2101 gaacgacagg acggggtggg gtggggggtc ttatcttact gtggatgagt ctgttacgct 2160

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca gggtcaagga 2220

2161 acgttaaagg agtaaaataa tcctttcctg tcaccctcac cgtggaaggt cccagttcct 2220

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag tcgaggtcta 2280

2221 tccgtgcccc ctccccgttt gttgtctacc gaccgttgat cttccgtgtc agctccagat 2280

.......... .......... ORF 1 . BGH_rev_primer BGHrev_primer .

NotI(2283),EagI(2283) XbaI(2290) ........ .......... ..........

2281 gcggccgctc tagatcaggc accgggcttg cgggtcatgc accaggtcgc gcggtccttc 2340

2281 cgccggcgag atctagtccg tggcccgaac gcccagtacg tggtccagcg cgccaggaag 2340

.......... .......... ORF 1 ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gggcacctcg acgtcggcgg tgacggtgaa gccgagccgc tcgtagaagg ggaggttgcg 2400

2341 cccgtggagc tgcagccgcc actgccactt cggctcggcg agcatcttcc cctccaacgc 2400

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... EagI(2443) ..........

2401 gggcgcggag gtctccagga aggcgggcac cccggcgcgc tcggccgcct ccactccggg 2460

2401 cccgcgcctc cagaggtcct tccgcccgtg gggccgcgcg agccggcgga ggtgaggccc 2460

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gagcacgacg gcgctgccca gacccttgcc ctggtggtcg ggcgagacgc cgacggtggc 2520

2461 ctcgtgctgc cgcgacgggt ctgggaacgg gaccaccagc ccgctctgcg gctgccaccg 2520

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 caggaaccac gcgggctcct tgggccggtg cggcgccagg aggccttcca tctgttgctg 2580

2521 gtccttggtg cgcccgagga acccggccac gccgcggtcc tccggaaggt agacaacgac 2580

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgcggccagc cgggaaccgc tcaactcggc catgcgcgga ccgatctcgg cgaacaccgc 2640

2581 gcgccggtcg gcccttggcg agttgagccg gtacgcgcct ggctagagcc gcttgtggcg 2640

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccccgcttcg acgctctccg gcgtggtcca gaccgccacc gcggcgccgt cgtccgcgac 2700

2641 ggggcgaagc tgcgagaggc cgcaccaggt ctggcggtgg cgccgcggca gcaggcgctg 2700

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccacaccttg ccgatgtcga gcccgacgcg cgtgaggaag agttcttgca gctcggtgac 2760

2701 ggtgtggaac ggctacagct cgggctgcgc gcactccttc tcaagaacgt cgagccactg 2760

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccgctcgatg tggcggtccg ggtcgacggt gtggcgcgtg gcggggtagt cggcgaacgc 2820

2761 ggcgagctac accgccaggc ccagctgcca caccgcgcac cgccccatca gccgcttgcg 2820

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ggcggcgagg gtgcgtacgg cccgggggac gtcgtcgcgg gtggcgaggc gcaccgtggg 2880

2821 ccgccgctcc cacgcatgcc gggccccctg cagcagcgcc caccgctccg cgtggcaccc 2880

.......... ......... ORF 1 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cttgtactct gtcattgatg tggtgggccc agggttggac tcgacgtctc ccgcaagttt 2940

2881 gaacatgaga cagtaactac accacccggg tcccaacctg agctgcagag ggcgttcaaa 2940

ORF 1 .......... ORF 2 .......... .......... ..........

.......... ......... PvuII(2962) ......... .......... ..........

2941 aagaaggtca aaattcaaca gctgaccgcc accgctgcca cctccgaatt cggcgccggt 3000

2941 ttcttccagt tttaagttgt cgactggcgg tggcgacggt ggaggcttaa gccgcggcca 3000

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ggagtggcgg ccctcggcgc gctcgtactg ttccacgatg gtgtagtcct cgttgtggga 3060

3001 cctcaccgcc gggagccgcg cgagcatgac aaggtgctac cacatcagga gcaacaccct 3060

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ggtgatgtcc agcttgatgt cggtcttgta ggcgccgggc agctgcacgg gcttcttggc 3120

3061 ccactacagg tcgaactaca gccagaacat ccgcggcccg tcgacgtgcc cgaagaaccg 3120

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 catgtaggtg gtcttgacct cggcgtcgta gtggccgccg tccttcagct tcagcctcat 3180

3121 gtacatccac cagaactgga gccgcagcat caccggcggc aggaagtcga agtcggagta 3180

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggtgg aggcctccca 3240

3181 gaactagagc gggaagtccc gcggcaggag ccccatgtag gcgagccacc tccggagggt 3240

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt 3300

3241 cgggtaccag aagaagacgt aatgccccgg cagcctcccc ttcaaccacg gcgcgtcgaa 3300

.......... .......... ORF 2 .......... .......... ..........

.......... .......... PstI(3330) .......... .......... ..........

3301 caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc 3360

3301 gtggaacatc tacttgagcg gcaggacgtc cctcctcagg acccagtgcc agtggtgcgg 3360

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa 3420

3361 cggcaggagc ttcaagtagt gcgcgagggt gaacttcggg agccccttcc tgtcgaagtt 3420

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtact ggaactgagg 3480

3421 catcagcccc tacagccgcc ccacgaagtg catccggaac ctcggcatga ccttgactcc 3480

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggacaggatg tcccaggcga agggcagggg gccgcccttg gtcaccttca gcttggcggt 3540

3481 cctgtcctac agggtccgct tcccgtcccc cggcgggaac cagtggaagt cgaaccgcca 3540

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt 3600

3541 gacccacggg agcatccccg ccgggagcgg gagcgggagc tagagcttga gcaccggcaa 3600

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cacggagccc tccatgcgca ccttgaagcg catgaactcc ttgatgacgt cctcggagga 3660

3601 gtgcctcggg aggtacgcgt ggaacttcgc gtacttgagg aactactgca ggagcctcct 3660

.......... .......... ORF 2 .......... .......... ..........

NcoI(3667) ClaI(3677) .......... .......... .......... ..........

3661 ggccaccatg gtggatcgat cctaggccac gtgttcacga cacctgaaat ggaagaaaaa 3720

3661 ccggtggtac cacctagcta ggatccggtg cacaagtgct gtggacttta ccttcttttt 3720

ORF 2 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aactttgaac cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaaggccaa 3780

3721 ttgaaacttg gtgacagact ccgaactctt acttggttct aggtttgagt ttttccggtt 3780

....... EF1a_fwd_primer ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 attccaagga gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag 3840

3781 taaggttcct cttaatgtag ttcacggttc gaccggattg aagtcagagg tgggtgagtc 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgtggggaaa ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa 3900

3841 acaccccttt gaggtagcgt attttgggga ggggggttgg atttctgctg catgaggttt 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agctccagaa ctaatcgagg tgcctggacg gcgcccggta ctccgtggag tcacatgaag 3960

3901 tcgaggtctt gattagctcc acggacctgc cgcgggccat gaggcacctc agtgtacttc 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgacggctga ggacggaaag gcccctttcc tttgtgtggg tgactcaccc gcccgctctc 4020

3961 gctgccgact cctgcctttc cggggaaagg aaacacaccc actgagtggg cgggcgagag 4020

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccgagcgccg cgtcctccat tttgagcccc ctggagcagg gccgggaagc ggccatcttt 4080

4021 ggctcgcggc gcaggaggta aaactcgggg gacctcgtcc cggcccttcg ccggtagaaa 4080

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccgctcacgc aactggtgcc gaccgggcca gccttgccgc ccagggcggg gcgatacacg 4140

4081 ggcgagtgcg ttgaccacgg ctggcccggt cggaacggcg ggtcccgccc cgctatgtgc 4140

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gcggcgcgag gccaggcacc agagcaggcc ggccagcttg agactacccc cgtccgattc 4200

4141 cgccgcgctc cggtccgtgg tctcgtccgg ccggtcgaac tctgatgggg gcaggctaag 4200

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ApaI(4260)

4201 tcggtggccg cgctcgcagg ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc 4260

4201 agccaccggc gcgagcgtcc ggggcggagc ggcttgtaca cgcgaccctg cgtgcccggg 4260

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgtcgccgcc cgcggcccca aaaaccgaaa taccagtgtg cagatcctgg cccgcattta 4320

4261 gcagcggcgg gcgccggggt ttttggcttt atggtcacac gtctaggacc gggcgtaaat 4320

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 caagactatc ttgccagaaa aaaagcgtcg cagcaggtca tcaaaaattt taaatggcta 4380

4321 gttctgatag aacggtcttt ttttcgcagc gtcgtccagt agtttttaaa atttaccgat 4380

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gagacttatc gaaagcagcg agacaggcgc gaaggtgcca ccagattcgc acgcggcggc 4440

4381 ctctgaatag ctttcgtcgc tctgtccgcg cttccacggt ggtctaagcg tgcgccgccg 4440

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cccagcgccc aggccaggcc tcaactcaag cacgaggcga aggggctcct aaagcgcaag 4500

4441 gggtcgcggg tccggtccgg agttgagttc gtgctccgct tccccgagga tttcgcgttc 4500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcccgcccct ggctccagct cgggatcaag aatcacgtac tggagccagg tggaagtaat 4560

4501 cgggcgggga ccgaggtcga gccctagttc ttagtgcatg acctcggtcc accttcatta 4560

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tcaaggcacg caagggccat aacccgtaaa gaggccaggc ccgcgggaac cacacacggc 4620

4561 agttccgtgc gttcccggta ttgggcattt ctccggtccg ggcgcccttg gtgtgtgccg 4620

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acttacctgt gttctggcgg caaacccgtt gcgaaaaaga acgttcacgg cgactactgc 4680

4621 tgaatggaca caagaccgcc gtttgggcaa cgctttttct tgcaagtgcc gctgatgacg 4680

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 acttatatac ggttctcccc caccctcggg aaaaaggcgg agccagtaca cgacatcact 4740

4681 tgaatatatg ccaagagggg gtgggagccc tttttccgcc tcggtcatgt gctgtagtga 4740

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ttcccagttt accccgcgcc accttctcta ggcaccggtt caattgccga cccctccccc 4800

4741 aagggtcaaa tggggcgcgg tggaagagat ccgtggccaa gttaacggct ggggaggggg 4800

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 caacttctcg gggactgtgg gcgatgtgcg ctctgcccac tgacgggcac cggagcctca 4860

4801 gttgaagagc ccctgacacc cgctacacgc gagacgggtg actgcccgtg gcctcggagt 4860

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgctcgagag gccgctcgac gataaaataa aagattttat ttagtctcca gaaaaagggg 4920

4861 gcgagctctc cggcgagctg ctattttatt ttctaaaata aatcagaggt ctttttcccc 4920

.......... ORF 2 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag 4980

4921 ccttactttc tggggtggac atccaaaccg ttcgatcgaa ttcattgcgg taaaacgttc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gcatggaaaa atacataact gagaatagaa aagttcagat caaggtcagg aacagaggaa 5040

4981 cgtacctttt tatgtattga ctcttatctt ttcaagtcta gttccagtcc ttgtctcctt 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 caggtcgacc aaggtcgggc aggaagaggg cctatttccc atgattcctt catatttgca 5100

5041 gtccagctgg ttccagcccg tccttctccc ggataaaggg tactaaggaa gtataaacgt 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat 5160

5101 atatgctatg ttccgacaat ctctctatta atcttaatta aactgacatt tgtgtttcta 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa 5220

5161 taatcatgtt ttatgcactg catctttcat tattaaagaa cccatcaaac gtcaaaattt 5220

.......... .......... .......... .......... .......... ..........

.......... .......... NdeI(5245) .......... .......... ..........

5221 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtact ctatcattga 5280

5221 taatacaaaa ttttacctga tagtatacga atggcattga actttcatga gatagtaact 5280

.......... ........ LKO1_5_primer ........ .......... ..........

.......... ......... BglII(5309) ........ BamHI(5323) .........

5281 tagagttata tatcttgtgg aaaggacaga tctccgcatg cggatccagg aacaatggca 5340

5281 atctcaatat atagaacacc tttcctgtct agaggcgtac gcctaggtcc ttgttaccgt 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gctttcttgc catcaaagct gctgatggca catatattct taatggtgac tacactttgt 5400

5341 cgaaagaacg gtagtttcga cgactaccgt gtatataaga attaccactg atgtgaaaca 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ccaccttaga gcaagacatt atgtacaaag gtgttgtctt gaggtacagc ggctcctctg 5460

5401 ggtggaatct cgttctgtaa tacatgtttc cacaacagaa ctccatgtcg ccgaggagac 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cggcattgga aagaattcgc agctttagcc ctctcaaaga gcccttgacc atccaggttc 5520

5461 gccgtaacct ttcttaagcg tcgaaatcgg gagagtttct cgggaactgg taggtccaag 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ttactgtggg caatgccctt cgacctaaaa ttaaatacac ctacttcgta aagaagaaga 5580

5521 aatgacaccc gttacgggaa gctggatttt aatttatgtg gatgaagcat ttcttcttct 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aggaatcttt caatgctatc cccacttttt cagcatgggt cattgaagag tggggcgaat 5640

5581 tccttagaaa gttacgatag gggtgaaaaa gtcgtaccca gtaacttctc accccgctta 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 gttctaagtc atgtgaattg ggttggcaga gaagactggt agaatgccga gacatcacct 5700

5641 caagattcag tacacttaac ccaaccgtct cttctgacca tcttacggct ctgtagtgga 5700

.......... .......... .......... .......... .......... ..........

.......... ........ HindIII(5729) ........ .......... ..........

5701 gcatgctttt tccatacttt ttttggcaag cttacgcgtg agcccacaac ccctcactcg 5760

5701 cgtacgaaaa aggtatgaaa aaaaccgttc gaatgcgcac tcgggtgttg gggagtgagc 5760

.......... .......... .......... .......... .......... ..........

.......... ......... XmaI(5791),SmaI(5793) KpnI(5799) .........

5761 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct 5820

5761 cccgcggtca ggaggctaac tgactcagcg ggcccatggg cacataggtt atttgggaga 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg 5880

5821 acgtcaacgt aggctgaaca ccagagcgac aaggaaccct cccagaggag actcactaac 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 actacccgtc agcgggggtc tttcacatgc agcatgtatc aaaattaatt tggttttttt 5940

5881 tgatgggcag tcgcccccag aaagtgtacg tcgtacatag ttttaattaa accaaaaaaa 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tcttaagtat ttacattaaa tggccatagt agttcattat ggacagcgca gaaagagctg 6000

5941 agaattcata aatgtaattt accggtatca tcaagtaata cctgtcgcgt ctttctcgac 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gggagaattg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 6060

6001 ccctcttaac actttaacaa taggcgagtg ttaaggtgtg ttgtatgctc ggccttcgta 6060

.......... .......... .......... ......... lac_promoter .........

.......... .......... .......... .......... .......... ..........

6061 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 6120

6061 tttcacattt cggaccccac ggattactca ctcgattgag tgtaattaac gcaacgcgag 6120

lac_promoter ........ .......... .......... .......... ..........

.......... .......... ......... PvuII(6158) ......... ..........

6121 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 6180

6121 tgacgggcga aaggtcagcc ctttggacag cacggtcgac gtaattactt agccggttgc 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 6240

6181 gcgcccctct ccgccaaacg cataacccgc gagaaggcga aggagcgagt gactgagcga 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 6300

6241 cgcgagccag caagccgacg ccgctcgcca tagtcgagtg agtttccgcc attatgccaa 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 6360

6301 taggtgtctt agtcccctat tgcgtccttt cttgtacact cgttttccgg tcgttttccg 6360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 6420

6361 gtccttggca tttttccggc gcaacgaccg caaaaaggta tccgaggcgg ggggactgct 6420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 6480

6421 cgtagtgttt ttagctgcga gttcagtctc caccgctttg ggctgtcctg atatttctat 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 6540

6481 ggtccgcaaa gggggacctt cgagggagca cgcgagagga caaggctggg acggcgaatg 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 6600

6541 gcctatggac aggcggaaag agggaagccc ttcgcaccgc gaaagagtat cgagtgcgac 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 6660

6601 atccatagag tcaagccaca tccagcaagc gaggttcgac ccgacacacg tgcttggggg 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 6720

6661 gcaagtcggg ctggcgacgc ggaataggcc attgatagca gaactcaggt tgggccattc 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 6780

6721 tgtgctgaat agcggtgacc gtcgtcggtg accattgtcc taatcgtctc gctccataca 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 6840

6781 tccgccacga tgtctcaaga acttcaccac cggattgatg ccgatgtgat cttcctgtca 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 6900

6841 taaaccatag acgcgagacg acttcggtca atggaagcct ttttctcaac catcgagaac 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 6960

6901 taggccgttt gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 7020

6961 cgcgtctttt tttcctagag ttcttctagg aaactagaaa agatgcccca gactgcgagt 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 7080

7021 caccttgctt ttgagtgcaa ttccctaaaa ccagtactct aatagttttt cctagaagtg 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 ctagatcctt ttaaattaaa aatgaagttt gcgcaaatca atctaaagta tatatgagta 7140

7081 gatctaggaa aatttaattt ttacttcaaa cgcgtttagt tagatttcat atatactcat 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7200

7141 ttgaaccaga ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc gctagacaga 7200

.......... .......... .......... bla ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

7201 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7260

7201 taaagcaagt aggtatcaac ggactgaggg gcagcacatc tattgatgct atgccctccc 7260

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7320

7261 gaatggtaga ccggggtcac gacgttacta tggcgctctg ggtgcgagtg gccgaggtct 7320

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 7380

7321 aaatagtcgt tatttggtcg gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa 7380

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 7440

7381 taggcggagg taggtcagat aattaacaac ggcccttcga tctcattcat caagcggtca 7440

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 7500

7441 attatcaaac gcgttgcaac aacggtaacg atgtccgtag caccacagtg cgagcagcaa 7500

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 7560

7501 accataccga agtaagtcga ggccaagggt tgctagttcc gctcaatgta ctagggggta 7560

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 7620

7561 caacacgttt tttcgccaat cgaggaagcc aggaggctag caacagtctt cattcaaccg 7620

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 7680

7621 gcgtcacaat agtgagtacc aataccgtcg tgacgtatta agagaatgac agtacggtag 7680

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... ScaI(7710) .......... .......... ..........

7681 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 7740

7681 gcattctacg aaaagacact gaccactcat gagttggttc agtaagactc ttatcacata 7740

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag 7800

7741 cgccgctggc tcaacgagaa cgggccgcag ttgtgcccta ttatggcgcg gtgtatcgtc 7800

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 7860

7801 ttgaaatttt cacgagtagt aaccttttgc aagaagcccc gcttttgaga gttcctagaa 7860

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 7920

7861 tggcgacaac tctaggtcaa gctacattgg gtgagcacgt gggttgacta gaagtcgtag 7920

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 7980

7921 aaaatgaaag tggtcgcaaa gacccactcg tttttgtcct tccgttttac ggcgtttttt 7980

.......... .......... bla ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8040

7981 cccttattcc cgctgtgcct ttacaactta tgagtatgag aaggaaaaag ttataataac 8040

.......... bla ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8041 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8100

8041 ttcgtaaata gtcccaataa cagagtactc gcctatgtat aaacttacat aaatcttttt 8100

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

8101 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccaccagctt tgctcttagg 8160

8101 atttgtttat ccccaaggcg cgtgtaaagg ggcttttcac ggtggtcgaa acgagaatcc 8160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 agtttcctaa tacatcccaa actcaaatat ataaagcatt tgacttgttc tatgc..... 8215

8161 tcaaaggatt atgtagggtt tgagtttata tatttcgtaa actgaacaag atacg..... 8215

.......... .......... .......... .......... .......... ..........

References

Reference number: [1] Reference positions: 1-8215 Reference author: Micklem D.R., Lorens J.B.; Reference title: "Retroviral vectors for expression of shRNAs"; Reference location: Unpublished.
Reference number: [2] Reference positions: 1-8215 Reference author: Micklem D.R., Lorens J.B.; Reference title: ; Reference location: Submitted (24-JAN-2008) to the EMBL/GenBank/DDBJ databases. Reference location: Biomedicine, University of Bergen, Jonas Lies vei 91, Bergen 5009, Norway