Sequence of L3691

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Sequence of L3691

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Sequence of L3691 : 4634 BP; 1303 A; 1037 C; 1008 G; 1286 T; 0 other;

.......... PstI(17) XbaI(25) SacII(34) .......... ..........

1 aagcttgcat gcctgcaggt cgactctaga ccgcggtttt ggcgcgccaa atgtaaacct 60

1 ttcgaacgta cggacgtcca gctgagatct ggcgccaaaa ccgcgcggtt tacatttgga 60

.......... .......... .......... .......... .......... mec7.-836

.......... .......... .......... .......... .......... ..........

61 gtcatttctg tgtatttcag caccgcgaag agcaccataa acagctacaa caagagcacg 120

61 cagtaaagac acataaagtc gtggcgcttc tcgtggtatt tgtcgatgtt gttctcgtgc 120

mec7.-836 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 aacgtcggct ccaacagaaa aagctgcaaa acgctggtac tcaccacatg ctctgataat 180

121 ttgcagccga ggttgtcttt ttcgacgttt tgcgaccatg agtggtgtac gagactatta 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgccatttcc ttcgatttct gactttcaat tgtgatatga taacctcaaa atctccattc 240

181 acggtaaagg aagctaaaga ctgaaagtta acactatact attggagttt tagaggtaag 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aaaatgaaaa ctcttaaatt taagagcttt caacaccccg cactttccca cctcatcgtt 300

241 ttttactttt gagaatttaa attctcgaaa gttgtggggc gtgaaagggt ggagtagcaa 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tttcccttct atcttatcaa ttttttgtat tttgtgtcag tttgctatat cctcatcacc 360

301 aaagggaaga tagaatagtt aaaaaacata aaacacagtc aaacgatata ggagtagtgg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttttttaccg tttaatctct ctatagtatt gttcgggtgt ttcaagatga aacgttttgt 420

361 aaaaaatggc aaattagaga gatatcataa caagcccaca aagttctact ttgcaaaaca 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gtgtagcatt tttgaaacac agcgcgaaaa atgcagaaac tatcatccac aatgcattaa 480

421 cacatcgtaa aaactttgtg tcgcgctttt tacgtctttg atagtaggtg ttacgtaatt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gtgcactcat tgtgatgact cctaaccccc tcgccccacc agttgtctct tcacaaattc 540

481 cacgtgagta acactactga ggattggggg agcggggtgg tcaacagaga agtgtttaag 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 atggcacaat tcaaaaatgg ctttttgtgt tggatcttga tctcgtctct ttccttcttc 600

541 taccgtgtta agtttttacc gaaaaacaca acctagaact agagcagaga aaggaagaag 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttcgtttct ctcggaccac tcattattcg aatgccatgc atttgttgat gatgcgcagc 660

601 aaagcaaaga gagcctggtg agtaataagc ttacggtacg taaacaacta ctacgcgtcg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... SacI(714)

661 gatggtcgca cacactacaa cagatatgat ggtggctgtg cgtggtgcag agctcatgaa 720

661 ctaccagcgt gtgtgatgtt gtctatacta ccaccgacac gcaccacgtc tcgagtactt 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 taagtgatgg ggcaccgaga tgtgactcct cccattattc gtcttgagtc gcatcttttg 780

721 attcactacc ccgtggctct acactgagga gggtaataag cagaactcag cgtagaaaac 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tcttggtccg ttgtcagtcg agcctcaagg cagccgttcc gccagtgatc gcctgctaag 840

781 agaaccaggc aacagtcagc tcggagttcc gtcggcaagg cggtcactag cggacgattc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 aagttgtagg tgtagaggaa gaaacgtcgg gtccaaattt caagcaacat ccgcgagatc 900

841 ttcaacatcc acatctcctt ctttgcagcc caggtttaaa gttcgttgta ggcgctctag 900

.......... .......... .......... mec7.Tsa .......... ..........

BamHI(903) NotI(919) .......... .......... .......... ..........

901 gtggatccta tcgattcgcg gccgctgtac acccgggtgc cctatagtga gtcgtattgg 960

901 cacctaggat agctaagcgc cggcgacatg tgggcccacg ggatatcact cagcataacc 960

.......... .......... .......... .......... T7_pro ..........

AgeI(962),KpnI(963) .......... .......... .......... ..........

961 taccggtaga aaaaatgagt aaaggagaag aacttttcac tggagttgtc ccaattcttg 1020

961 atggccatct tttttactca tttcctcttc ttgaaaagtg acctcaacag ggttaagaac 1020

.......... .......... ...... GFP(w/ gaps) ORF 3 ...... ..........

.......... .......... .......... .......... .......... ..........

1021 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg 1080

1021 aacttaatct accactacaa ttacccgtgt ttaaaagaca gtcacctctc ccacttccac 1080

.......... ...... GFP(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... NcoI(1140)

1081 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc 1140

1081 tacgttgtat gccttttgaa tgggaattta aataaacgtg atgacctttt gatggacaag 1140

.......... ...... GFP(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 catgggtaag tttaaacata tatatactaa ctaaccctga ttatttaaat tttcagccaa 1200

1141 gtacccattc aaatttgtat atatatgatt gattgggact aataaattta aaagtcggtt 1200

.......... ...... GFP(w/ gaps) ORF 3 SynIVSR ....... ..........

.......... .......... .......... XhoI(1238) .......... ..........

1201 cacttgtcac tactttctgt tatggtgttc aatgcttctc gagataccca gatcatatga 1260

1201 gtgaacagtg atgaaagaca ataccacaag ttacgaagag ctctatgggt ctagtatact 1260

.......... .. GFP(w/ gaps) ORF 3 gfp.S65C .. .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aacagcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat 1320

1261 ttgtcgtact gaaaaagttc tcacggtacg ggcttccaat acatgtcctt tcttgatata 1320

.......... ...... GFP(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttttcaaaga tgacgggaac tacaagacac gtaagtttaa acagttcggt actaactaac 1380

1321 aaaagtttct actgcccttg atgttctgtg cattcaaatt tgtcaagcca tgattgattg 1380

.......... ...... GFP(w/ gaps) ORF 3 ...... SynIVS.S ..........

.......... .......... .......... .......... .......... ..........

1381 catacatatt taaattttca ggtgctgaag tcaagtttga aggtgatacc cttgttaata 1440

1381 gtatgtataa atttaaaagt ccacgacttc agttcaaact tccactatgg gaacaattat 1440

....... SynIVSS GFP(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg 1500

1441 cttagctcaa ttttccataa ctaaaatttc ttctaccttt gtaagaacct gtgtttaacc 1500

.......... ...... GFP(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca 1560

1501 ttatgttgat attgagtgtg ttacatatgt agtaccgtct gtttgttttc ttaccttagt 1560

.......... ...... GFP(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aagttgtaag tttaaacatg attttactaa ctaactaatc tgatttaaat tttcagaact 1620

1561 ttcaacattc aaatttgtac taaaatgatt gattgattag actaaattta aaagtcttga 1620

.......... ...... GFP(w/ gaps) ORF 3 SynIVST ....... ..........

.......... .......... .......... .......... .......... ..........

1621 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa 1680

1621 agttttaatc tgtgttgtaa cttctacctt cgcaagttga tcgtctggta atagttgttt 1680

.......... ...... GFP(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat 1740

1681 tatgaggtta accgctaccg ggacaggaaa atggtctgtt ggtaatggac aggtgtgtta 1740

.......... ...... GFP(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa 1800

1741 gacgggaaag ctttctaggg ttgcttttct ctctggtgta ccaggaagaa ctcaaacatt 1800

.......... ...... GFP(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cagctgctgg gattacacat ggcatggacg aactatacaa aagcccacaa gctttggaat 1860

1801 gtcgacgacc ctaatgtgta ccgtacctgc ttgatatgtt ttcgggtgtt cgaaacctta 1860

......... GFP(w/ gaps) ORF 3 ......... .......... ..........

NheI(1863) .......... .......... .......... .......... ..........

1861 tcgctagccg gccatacaag taatccggat gatcgacgcc aacgtcgttg aattttcaaa 1920

1861 agcgatcggc cggtatgttc attaggccta ctagctgcgg ttgcagcaac ttaaaagttt 1920

.......... ORF 3 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttttaaatac tgaatatttg ttttttttcc tattatttat ttattctctt tgtgtttttt 1980

1921 aaaatttatg acttataaac aaaaaaaagg ataataaata aataagagaa acacaaaaaa 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttcttgcttt ctaaaaaatt aattcaatcc aaatctaaac attttttttt ctctttccgt 2040

1981 aagaacgaaa gattttttaa ttaagttagg tttagatttg taaaaaaaaa gagaaaggca 2040

.......... .......... let858.3' .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctcccaattc gtattccgct cctctcatct gaacacaatg tgcaagttta tttatcttct 2100

2041 gagggttaag cataaggcga ggagagtaga cttgtgttac acgttcaaat aaatagaaga 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cgctttcatt tcattaggac gtggggggaa ttggtggaag ggggaaacac acaaaaggat 2160

2101 gcgaaagtaa agtaatcctg cacccccctt aaccaccttc cccctttgtg tgttttccta 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gatggaaatg aaataaggac acacaatatg caacaacatt caattcagaa atatggagga 2220

2161 ctacctttac tttattcctg tgtgttatac gttgttgtaa gttaagtctt tatacctcct 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aggtttaaaa gaaaacataa aaatatatag aggaggaagg aaaactagta aaaaataagc 2280

2221 tccaaatttt cttttgtatt tttatatatc tcctccttcc ttttgatcat tttttattcg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ApaI(2322) ..........

2281 aaagaaatta ggcgaacgat gagaattgtc ctcgcttggg cccaaaaggc cttttttttt 2340

2281 tttctttaat ccgcttgcta ctcttaacag gagcgaaccc gggttttccg gaaaaaaaaa 2340

.......... .......... .......... .......... .......... ..........

NarI(2343) AatII(2352) ........ .......... .......... ..........

2341 tggcgccgac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 2400

2341 accgcggctg cagtccaccg tgaaaagccc ctttacacgc gccttgggga taaacaaata 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 2460

2401 aaaagattta tgtaagttta tacataggcg agtactctgt tattgggact atttacgaag 2460

.......... .......... Amp_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct 2520

2461 ttattataac tttttccttc tcatactcat aagttgtaaa ggcacagcgg gaataaggga 2520

.......... .......... .......... ....... ORF 3 Ampicillin .......

.......... .......... .......... .......... .......... ..........

2521 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag 2580

2521 aaaaacgccg taaaacggaa ggacaaaaac gagtgggtct ttgcgaccac tttcattttc 2580

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta 2640

2581 tacgacttct agtcaaccca cgtgctcacc caatgtagct tgacctagag ttgtcgccat 2640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc 2700

2641 tctaggaact ctcaaaagcg gggcttcttg caaaaggtta ctactcgtga aaatttcaag 2700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca 2760

2701 acgatacacc gcgccataat agggcataac tgcggcccgt tctcgttgag ccagcggcgt 2760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg 2820

2761 atgtgataag agtcttactg aaccaactca tgagtggtca gtgtcttttc gtagaatgcc 2820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg 2880

2821 taccgtactg tcattctctt aatacgtcac gacggtattg gtactcacta ttgtgacgcc 2880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca 2940

2881 ggttgaatga agactgttgc tagcctcctg gcttcctcga ttggcgaaaa aacgtgttgt 2940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa 3000

2941 accccctagt acattgagcg gaactagcaa cccttggcct cgacttactt cggtatggtt 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa 3060

3001 tgctgctcgc actgtggtgc tacggacatc gttaccgttg ttgcaacgcg tttgataatt 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata 3120

3061 gaccgcttga tgaatgagat cgaagggccg ttgttaatta tctgacctac ctccgcctat 3120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat 3180

3121 ttcaacgtcc tggtgaagac gcgagccggg aaggccgacc gaccaaataa cgactattta 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc 3240

3181 gacctcggcc actcgcaccc agagcgccat agtaacgtcg tgaccccggt ctaccattcg 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata 3300

3241 ggagggcata gcatcaatag atgtgctgcc cctcagtccg ttgataccta cttgctttat 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt 3360

3301 ctgtctagcg actctatcca cggagtgact aattcgtaac cattgacagt ctggttcaaa 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga 3420

3361 tgagtatata tgaaatctaa ctaaattttg aagtaaaaat taaattttcc tagatccact 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag 3480

3421 tctaggaaaa actattagag tactggtttt agggaattgc actcaaaagc aaggtgactc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa 3540

3481 gcagtctggg gcatcttttc tagtttccta gaagaactct aggaaaaaaa gacgcgcatt 3540

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3541 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag 3600

3541 agacgacgaa cgtttgtttt tttggtggcg atggtcgcca ccaaacaaac ggcctagttc 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg 3660

3601 tcgatggttg agaaaaaggc ttccattgac cgaagtcgtc tcgcgtctat ggtttatgac 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat 3720

3661 aggaagatca catcggcatc aatccggtgg tgaagttctt gagacatcgt ggcggatgta 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta 3780

3721 tggagcgaga cgattaggac aatggtcacc gacgacggtc accgctattc agcacagaat 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg 3840

3781 ggcccaacct gagttctgct atcaatggcc tattccgcgt cgccagcccg acttgccccc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc 3900

3841 caagcacgtg tgtcgggtcg aacctcgctt gctggatgtg gcttgactct atggatgtcg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa 3960

3901 cactcgtaac tctttcgcgg tgcgaagggc ttccctcttt ccgcctgtcc ataggccatt 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc 4020

3961 cgccgtccca gccttgtcct ctcgcgtgct ccctcgaagg tccccctttg cggaccatag 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt 4080

4021 aaatatcagg acagcccaaa gcggtggaga ctgaactcgc agctaaaaac actacgagca 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct 4140

4081 gtccccccgc ctcggatacc tttttgcggt cgttgcgccg gaaaaatgcc aaggaccgga 4140

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc 4200

4141 aaacgaccgg aaaacgagtg tacaagaaag gacgcaatag gggactaaga cacctattgg 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg 4260

4201 cataatggcg gaaactcact cgactatggc gagcggcgtc ggcttgctgg ctcgcgtcgc 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt 4320

4261 tcagtcactc gctccttcgc cttctcgcgg gttatgcgtt tggcggagag gggcgcgcaa 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 4380

4321 ccggctaagt aattacgtcg accgtgctgt ccaaagggct gacctttcgc ccgtcactcg 4380

.......... .......... lacI.C .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 4440

4381 cgttgcgtta attacactca atcgagtgag taatccgtgg ggtccgaaat gtgaaatacg 4440

.......... .......... .......... .......... .......... lac_pro

.......... .......... .......... .......... .......... ..........

4441 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 4500

4441 aaggccgagc atacaacaca ccttaacact cgcctattgt taaagtgtgt cctttgtcga 4500

lac_pro .......... .......... .......... .......... M13r_pri

.......... .......... .......... .......... .......... ..........

4501 atgaccatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca 4560

4501 tactggtact aatgcggttc gacattcaaa tttgtactag aatgattgat tgataagagt 4560

M13r_pri .......... .......... .......... decoy ..........

.......... .......... .......... .......... .......... ..........

4561 tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat acgctaacaa 4620

4561 aaatttaaaa gtctcgaatt tttaccgact ttagtgagtg ttgctaccta tgcgattgtt 4620

decoy .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cttggaaatg aaat...... .......... .......... .......... .......... 4634

4621 gaacctttac ttta...... .......... .......... .......... .......... 4634

.......... .......... .......... .......... .......... ..........