Sequence of L4440

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Sequence of L4440

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Sequence of L4440 : 2790 BP; 675 A; 707 C; 696 G; 712 T; 0 other;

.......... .......... .......... .......... BglII(47) ..........

1 aacctggctt atcgaaatta atacgactca ctatagggag accggcagat ctgatatcat 60

1 ttggaccgaa tagctttaat tatgctgagt gatatccctc tggccgtcta gactatagta 60

.......... ......... T7_promoter ......... .......... ..........

...... SacI(75) SacII(82) NotI(88) XbaI(95) ...... AgeI(113)

61 cgatgaattc gagctccacc gcggtggcgg ccgctctaga actagtggat ccaccggttc 120

61 gctacttaag ctcgaggtgg cgccaccgcc ggcgagatct tgatcaccta ggtggccaag 120

.......... .......... .......... .......... .......... ..........

NheI(125) .......... ..... SmaI(151) PstI(159) ..... ..........

121 catggctagc cacgtgacgc gtggatcccc cgggctgcag gaattcgata tcaagcttat 180

121 gtaccgatcg gtgcactgcg cacctagggg gcccgacgtc cttaagctat agttcgaata 180

.......... .......... .......... .......... .......... ..........

.......... XhoI(194) ApaI(207) KpnI(213) .......... ..........

181 cgataccgtc gacctcgagg gggggcccgg tacccaattc gccctatagt gagtcgtatt 240

181 gctatggcag ctggagctcc cccccgggcc atgggttaag cgggatatca ctcagcataa 240

.......... .......... .......... ......... T7_promoter .........

.......... .......... .......... .......... .......... ..........

241 acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 300

241 tgcgcgcgag tgaccggcag caaaatgttg cagcactgac ccttttggga ccgcaatggg 300

........ M13f20_primer ........ lacZ_a .......... ..........

.......... .......... .......... PvuII(337) .......... ..........

301 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 360

301 ttgaattagc ggaacgtcgt gtagggggaa agcggtcgac cgcattatcg cttctccggg 360

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta 420

361 cgtggctagc gggaagggtt gtcaacgcgt cggacttacc gcttaccctg cgcgggacat 420

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 480

421 cgccgcgtaa ttcgcgccgc ccacaccacc aatgcgcgtc gcactggcga tgtgaacggt 480

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

481 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 540

481 cgcgggatcg cgggcgagga aagcgaaaga agggaaggaa agagcggtgc aagcggccga 540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 600

541 aaggggcagt tcgagattta gcccccgagg gaaatcccaa ggctaaatca cgaaatgccg 600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 660

601 tggagctggg gttttttgaa ctaatcccac taccaagtgc atcacccggt agcgggacta 660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 720

661 tctgccaaaa agcgggaaac tgcaacctca ggtgcaagaa attatcacct gagaacaagg 720

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 780

721 tttgaccttg ttgtgagttg ggatagagcc agataagaaa actaaatatt ccctaaaacg 780

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 840

781 gctaaagccg gataaccaat tttttactcg actaaattgt ttttaaattg cgcttaaaat 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc 900

841 tgttttataa ttgcgaatgt taaatccacc gtgaaaagcc cctttacacg cgccttgggg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 960

901 ataaacaaat aaaaagattt atgtaagttt atacataggc gagtactctg ttattgggac 960

.......... .......... ......... Amp_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

961 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 1020

961 tatttacgaa gttattataa ctttttcctt ctcatactca taagttgtaa aggcacagcg 1020

.......... .......... .......... .... Orf frame 2 Ampicillin ....

.......... .......... .......... .......... .......... ..........

1021 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 1080

1021 ggaataaggg aaaaaacgcc gtaaaacgga aggacaaaaa cgagtgggtc tttgcgacca 1080

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 1140

1081 ctttcatttt ctacgacttc tagtcaaccc acgtgctcac ccaatgtagc ttgacctaga 1140

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 1200

1141 gttgtcgcca ttctaggaac tctcaaaagc ggggcttctt gcaaaaggtt actactcgtg 1200

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact 1260

1201 aaaatttcaa gacgatacac cgcgccataa tagggcataa ctgcggcccg ttctcgttga 1260

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 1320

1261 gccagcggcg tatgtgataa gagtcttact gaaccaactc atgagtggtc agtgtctttt 1320

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 1380

1321 cgtagaatgc ctaccgtact gtcattctct taatacgtca cgacggtatt ggtactcact 1380

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 1440

1381 attgtgacgc cggttgaatg aagactgttg ctagcctcct ggcttcctcg attggcgaaa 1440

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 1500

1441 aaacgtgttg taccccctag tacattgagc ggaactagca acccttggcc tcgacttact 1500

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg 1560

1501 tcggtatggt ttgctgctcg cactgtggtg ctacggacat cgttaccgtt gttgcaacgc 1560

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 1620

1561 gtttgataat tgaccgcttg atgaatgaga tcgaagggcc gttgttaatt atctgaccta 1620

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 1680

1621 cctccgccta tttcaacgtc ctggtgaaga cgcgagccgg gaaggccgac cgaccaaata 1680

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 1740

1681 acgactattt agacctcggc cactcgcacc cagagcgcca tagtaacgtc gtgaccccgg 1740

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 1800

1741 tctaccattc gggagggcat agcatcaata gatgtgctgc ccctcagtcc gttgatacct 1800

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 1860

1801 acttgcttta tctgtctagc gactctatcc acggagtgac taattcgtaa ccattgacag 1860

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 1920

1861 tctggttcaa atgagtatat atgaaatcta actaaatttt gaagtaaaaa ttaaattttc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 1980

1921 ctagatccac ttctaggaaa aactattaga gtactggttt tagggaattg cactcaaaag 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 2040

1981 caaggtgact cgcagtctgg ggcatctttt ctagtttcct agaagaactc taggaaaaaa 2040

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2041 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 2100

2041 agacgcgcat tagacgacga acgtttgttt ttttggtggc gatggtcgcc accaaacaaa 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 2160

2101 cggcctagtt ctcgatggtt gagaaaaagg cttccattga ccgaagtcgt ctcgcgtcta 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 2220

2161 tggtttatga caggaagatc acatcggcat caatccggtg gtgaagttct tgagacatcg 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 2280

2221 tggcggatgt atggagcgag acgattagga caatggtcac cgacgacggt caccgctatt 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 2340

2281 cagcacagaa tggcccaacc tgagttctgc tatcaatggc ctattccgcg tcgccagccc 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 2400

2341 gacttgcccc ccaagcacgt gtgtcgggtc gaacctcgct tgctggatgt ggcttgactc 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 2460

2401 tatggatgtc gcactcgata ctctttcgcg gtgcgaaggg cttccctctt tccgcctgtc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 2520

2461 cataggccat tcgccgtccc agccttgtcc tctcgcgtgc tccctcgaag gtcccccttt 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 2580

2521 gcggaccata gaaatatcag gacagcccaa agcggtggag actgaactcg cagctaaaaa 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 2640

2581 cactacgagc agtccccccg cctcggatac ctttttgcgg tcgttgcgcc ggaaaaatgc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 2700

2641 caaggaccgg aaaacgaccg gaaaacgagt gtacaagaaa ggacgcaata ggggactaag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 2760

2701 acacctattg gcataatggc ggaaactcac tcgactatgg cgagcggcgt cggcttgctg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgagcgcagc gagtcagtga gcgaggaagc .......... .......... .......... 2790

2761 gctcgcgtcg ctcagtcact cgctccttcg .......... .......... .......... 2790

.......... .......... .......... .......... .......... ..........