Sequence of L4817

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Sequence of L4817

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Sequence of L4817 : 6755 BP; 1988 A; 1389 C; 1397 G; 1981 T; 0 other;

......... HindIII(18) ......... .......... .......... ..........

1 atgaccatga ttacgccaag cttgggctgc aggtcggcta taataagttc ttgaataaaa 60

1 tactggtact aatgcggttc gaacccgacg tccagccgat attattcaag aacttatttt 60

.......... .......... .......... .......... myo3.-2341 ..........

.......... .......... .......... .......... .......... ..........

61 taattttccc gacaaaacat gagtatttct ttcgaaaata aaagtgcagg ctaattagag 120

61 attaaaaggg ctgttttgta ctcataaaga aagcttttat tttcacgtcc gattaatctc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 attattctgt aattaactgc ataatttgtc acgtgccata gttttacatt ccactacgtc 180

121 taataagaca ttaattgacg tattaaacag tgcacggtat caaaatgtaa ggtgatgcag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 atagttctta aaatactaat ctcctgaaaa tagaagtagg tgaagaaagt ttaattatca 240

181 tatcaagaat tttatgatta gaggactttt atcttcatcc acttctttca aattaatagt 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gttctaaaat gacaattgat ctttggaata tgttctgaaa ctaccgatca ttgaacagat 300

241 caagatttta ctgttaacta gaaaccttat acaagacttt gatggctagt aacttgtcta 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gctatttgaa tgatatagaa ttgtatattt gcaatttctg aaacgcgttc ttaaaggcac 360

301 cgataaactt actatatctt aacatataaa cgttaaagac tttgcgcaag aatttccgtg 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 acagattaat tcaaaagggt ctggccgcaa aaaggtttat ggtggccgat tttgagtttt 420

361 tgtctaatta agttttccca gaccggcgtt tttccaaata ccaccggcta aaactcaaaa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... BglII(472)

421 gtgtgtgatt gctttttcac aatcagtgtt ttcaggatta tgtgatgaac tagatcttca 480

421 cacacactaa cgaaaaagtg ttagtcacaa aagtcctaat acactacttg atctagaagt 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 agtttcgtta catttcatat gttttcggaa ctcacgaagt acatattggg tattgtgctc 540

481 tcaaagcaat gtaaagtata caaaagcctt gagtgcttca tgtataaccc ataacacgag 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 aaaaaattca gcaatcagct tcgctccgct gactttagaa cccaaaaaaa tagtatggcc 600

541 ttttttaagt cgttagtcga agcgaggcga ctgaaatctt gggttttttt atcataccgg 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aaactgactg tgttacgatc atttcaattt ttcaatacat atttaagatt tctaagagta 660

601 tttgactgac acaatgctag taaagttaaa aagttatgta taaattctaa agattctcat 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 agaaggtcaa aaactgttct ggaatacata tatatttttc aggttacaat tagtcaaaaa 720

661 tcttccagtt tttgacaaga ccttatgtat atataaaaag tccaatgtta atcagttttt 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtgcactgaa atatacgttt taatttcacg aataacccaa ttagttcaat gtatttttgg 780

721 cacgtgactt tatatgcaaa attaaagtgc ttattgggtt aatcaagtta cataaaaacc 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... BclI(823) ..........

781 tcaaccaacg ttaaagtttg gcttccaacc aattatcatt tctgatcaac cacaatgttt 840

781 agttggttgc aatttcaaac cgaaggttgg ttaatagtaa agactagttg gtgttacaaa 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tttctttatc tgcaagttaa ttttttattt ttatccagat gtttggcata tttttcaatt 900

841 aaagaaatag acgttcaatt aaaaaataaa aataggtcta caaaccgtat aaaaagttaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NarI(931) .......... ..........

901 cttcactagc gcccacttct tgcacttccg gcgccctgaa tctaatgcat ctgttgcaag 960

901 gaagtgatcg cgggtgaaga acgtgaaggc cgcgggactt agattacgta gacaacgttc 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aattgaaaga ccaatcaaca cattgttttc ttcacgagat actgaagaaa atgaataaaa 1020

961 ttaactttct ggttagttgt gtaacaaaag aagtgctcta tgacttcttt tacttatttt 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 acagagaaaa agagccatgt gattagtgac aactgttgct aacagatacc atagcttgga 1080

1021 tgtctctttt tctcggtaca ctaatcactg ttgacaacga ttgtctatgg tatcgaacct 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cttggtacgt gatggcaacg tatgggtcaa caaaaatgat tgcagagggg gtgcaaaaca 1140

1081 gaaccatgca ctaccgttgc atacccagtt gtttttacta acgtctcccc cacgttttgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtcaagtcga gaaaatatga aaaacagaaa acaaagaaca gaaaaatggg tttgagagtc 1200

1141 cagttcagct cttttatact ttttgtcttt tgtttcttgt ctttttaccc aaactctcag 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 agtataattt ataaaagaaa aattgtacat agaaattaac catttttgta gaagaagtta 1260

1201 tcatattaaa tattttcttt ttaacatgta tctttaattg gtaaaaacat cttcttcaat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttttcaagc atcgttaaaa attattcaaa gcaccttatt tcatatttaa ttttaaacat 1320

1261 aaaaagttcg tagcaatttt taataagttt cgtggaataa agtataaatt aaaatttgta 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggttaaatga acaacacggt gcgcaatcag gaaaacttga aatctgaaac tgttgttgtg 1380

1321 ccaatttact tgttgtgcca cgcgttagtc cttttgaact ttagactttg acaacaacac 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atcttcttcg caactgttca gatagcacta gtgtaatgtt aagagtgcgc gaatataatg 1440

1381 tagaagaagc gttgacaagt ctatcgtgat cacattacaa ttctcacgcg cttatattac 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gaatataatg gatcacacct cctgccatca ggtaaacgtc tctgttatca catatttcca 1500

1441 cttatattac ctagtgtgga ggacggtagt ccatttgcag agacaatagt gtataaaggt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 actattaaat ttttaccttt tacagtttta catttttttg aaaaaagtaa ctttttgtct 1560

1501 tgataattta aaaatggaaa atgtcaaaat gtaaaaaaac ttttttcatt gaaaaacaga 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tcaaaatccc tgacgaaaat atcaaatatt ttaatcgaga ctgcagagga accgattgat 1620

1561 agttttaggg actgctttta tagtttataa aattagctct gacgtctcct tggctaacta 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gatttggaaa atccagcttt acctgtgtaa gaactgaaaa gtttcataac cctagggtat 1680

1621 ctaaaccttt taggtcgaaa tggacacatt cttgactttt caaagtattg ggatcccata 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcccagttac attccccact ggctaacaat agcacccagt ttttcatcac ctttcttcaa 1740

1681 agggtcaatg taaggggtga ccgattgtta tcgtgggtca aaaagtagtg gaaagaagtt 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRV(1787) .........

1741 atttctcggc gatttgttaa aaacaaaatt tgtgtccctt ctctgatatc tctatgtctc 1800

1741 taaagagccg ctaaacaatt tttgttttaa acacagggaa gagactatag agatacagag 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taaacacaag ttcatcggaa aacgaaggag ggtaggtgtt ggttgggctc ccgaagtgaa 1860

1801 atttgtgttc aagtagcctt ttgcttcctc ccatccacaa ccaacccgag ggcttcactt 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aatagaagag caagaataga atattagaga gagagtgcag agagggcggg atagctcccg 1920

1861 ttatcttctc gttcttatct tataatctct ctctcacgtc tctcccgccc tatcgagggc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggattccgtt ttcttcttct ttatcttcaa cgatgatgtg tgtgcgtgtt gtatagattc 1980

1921 cctaaggcaa aagaagaaga aatagaagtt gctactacac acacgcacaa catatctaag 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgttgctccc ccacaactcg ctccgaaggc tcaatacaat tcaattgata ttggaggaga 2040

1981 acaacgaggg ggtgttgagc gaggcttccg agttatgtta agttaactat aacctcctct 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gcctaccgga gtgggaggat aagaagaaac ataagaagaa gaagaagaag aagcatgctt 2100

2041 cggatggcct caccctccta ttcttctttg tattcttctt cttcttcttc ttcgtacgaa 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctggtttttg atgctatgaa aacggcacaa aaagatgatt gaggtccctt ttcaatacct 2160

2101 gaccaaaaac tacgatactt ttgccgtgtt tttctactaa ctccagggaa aagttatgga 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tctctcatct ttcaaatccc attgaaacct aaaacttctc accacgcttt accattgttc 2220

2161 agagagtaga aagtttaggg taactttgga ttttgaagag tggtgcgaaa tggtaacaag 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tccaaaaact tatagcaatg tctataactt ttttatctct gaaaagcagt gttccatttt 2280

2221 aggtttttga atatcgttac agatattgaa aaaatagaga cttttcgtca caaggtaaaa 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tctttttcct attttatttc aattgtttct cacatttcgt ttggattctt tgcttgtcaa 2340

2281 agaaaaagga taaaataaag ttaacaaaga gtgtaaagca aacctaagaa acgaacagtt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccagcttctt cttccacttt taccgtctaa ttttcagggc agggagccat caaacccacg 2400

2341 ggtcgaagaa gaaggtgaaa atggcagatt aaaagtcccg tccctcggta gtttgggtgc 2400

.......... .......... myo3.Tsa .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 accactagat ccatctagag gatccccggg attggccaaa ggacccaaag gaggaccctt 2460

2401 tggtgatcta ggtagatctc ctaggggccc taaccggttt cctgggtttc ctcctgggaa 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggagggtacc ggtagaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa 2520

2461 cctcccatgg ccatcttttt tactcatttc ctcttcttga aaagtgacct caacagggtt 2520

.......... .......... ... GFP(w/ gaps) Orf frame 3 ... ..........

.......... .......... .......... .......... .......... ..........

2521 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg 2580

2521 aagaacaact taatctacca ctacaattac ccgtgtttaa aagacagtca cctctcccac 2580

.......... ... GFP(w/ gaps) Orf frame 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac 2640

2581 ttccactacg ttgtatgcct tttgaatggg aatttaaata aacgtgatga ccttttgatg 2640

.......... ... GFP(w/ gaps) Orf frame 3 ... .......... ..........

NcoI(2646) .......... .......... .......... .......... ..........

2641 ctgttccatg ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc 2700

2641 gacaaggtac ccattcaaat ttgtatatat atgattgatt gggactaata aatttaaaag 2700

.......... ... GFP(w/ gaps) Orf frame 3 SynIVSR .... ..........

.......... .......... .......... .......... .......... ..........

2701 agccaacact tgtcactact ttcggttatg gtcttcaatg cttcgccaga tacccagatc 2760

2701 tcggttgtga acagtgatga aagccaatac cagaagttac gaagcggtct atgggtctag 2760

.......... ... GFP(w/ gaps) Orf frame 3 gfpGLA .... ..........

.......... .......... .......... .......... .......... ..........

2761 atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa 2820

2761 tatactttgt cgtactgaaa aagttctcac ggtacgggct tccaatacat gtcctttctt 2820

.......... ... GFP(w/ gaps) Orf frame 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctatattttt caaagatgac gggaactaca agacacgtaa gtttaaacag ttcggtacta 2880

2821 gatataaaaa gtttctactg cccttgatgt tctgtgcatt caaatttgtc aagccatgat 2880

.......... ... GFP(w/ gaps) Orf frame 3 ... SynIVS.S ..........

.......... .......... .......... .......... .......... ..........

2881 actaaccata catatttaaa ttttcaggtg ctgaagtcaa gtttgaaggt gatacccttg 2940

2881 tgattggtat gtataaattt aaaagtccac gacttcagtt caaacttcca ctatgggaac 2940

.... SynIVSS GFP(w/ gaps) Orf frame 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca 3000

2941 aattatctta gctcaatttt ccataactaa aatttcttct acctttgtaa gaacctgtgt 3000

.......... ... GFP(w/ gaps) Orf frame 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg 3060

3001 ttaaccttat gttgatattg agtgtgttac atatgtagta ccgtctgttt gttttcttac 3060

.......... ... GFP(w/ gaps) Orf frame 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gaatcaaagt tgtaagttta aacatgattt tactaactaa ctaatctgat ttaaattttc 3120

3061 cttagtttca acattcaaat ttgtactaaa atgattgatt gattagacta aatttaaaag 3120

.......... ... GFP(w/ gaps) Orf frame 3 SynIVST .... ..........

.......... .......... .......... .......... .......... ..........

3121 agaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc 3180

3121 tcttgaagtt ttaatctgtg ttgtaacttc taccttcgca agttgatcgt ctggtaatag 3180

.......... ... GFP(w/ gaps) Orf frame 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcct 3240

3181 ttgttttatg aggttaaccg ctaccgggac aggaaaatgg tctgttggta atggacagga 3240

.......... ... GFP(w/ gaps) Orf frame 3 ... .......... gfp.T2Y

.......... .......... .......... .......... .......... ..........

3241 accaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt 3300

3241 tggttagacg ggaaagcttt ctagggttgc ttttctctct ggtgtaccag gaagaactca 3300

gfp.T2Y ... GFP(w/ gaps) Orf frame 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaatag cattcgtaga 3360

3301 aacattgtcg acgaccctaa tgtgtaccgt acctacttga tatgtttatc gtaagcatct 3360

.......... ... GFP(w/ gaps) Orf frame 3 ... .......... ..........

.......... .......... .......... .......... ......... EcoRI(3414)

3361 attgtaagtt taaactttct catactaact aacgcgctct atttaaattt tcagaattcc 3420

3361 taacattcaa atttgaaaga gtatgattga ttgcgcgaga taaatttaaa agtcttaagg 3420

.......... .......... .......... SynIVS.M .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggggccgc 3480

3421 ttgactcgcg gccagcgatg gtaatggttg aacagaccac agtttttatt atccccggcg 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgtcatcaga gtaagtttaa actgagttct actaactaac gagtaatatt taaattttca 3540

3481 acagtagtct cattcaaatt tgactcaaga tgattgattg ctcattataa atttaaaagt 3540

.......... .......... .......... SynIVS.L .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcatctcgcg cccgtgcctc tgacttctaa gtccaattac tcttcaacat ccctacatgc 3600

3541 cgtagagcgc gggcacggag actgaagatt caggttaatg agaagttgta gggatgtacg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tctttctccc tgtgctccca ccccctattt ttgttattat caaaaaactt ctcttaattt 3660

3601 agaaagaggg acacgagggt gggggataaa aacaataata gttttttgaa gagaattaaa 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctttgttttt tagcttcttt taagtcacct ctaacaatga aattgtgtag attcaaaaat 3720

3661 gaaacaaaaa atcgaagaaa attcagtgga gattgttact ttaacacatc taagttttta 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agaattaatt cgtaataaaa agtcgaaaaa aattgtgctc cctcccccca ttaataataa 3780

3721 tcttaattaa gcattatttt tcagcttttt ttaacacgag ggaggggggt aattattatt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ttctatccca aaatctacac aatgttctgt gtacacttct tatgtttttt acttctgata 3840

3781 aagatagggt tttagatgtg ttacaagaca catgtgaaga atacaaaaaa tgaagactat 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aatttttttg aaacatcata gaaaaaaccg cacacaaaat accttatcat atgttacgtt 3900

3841 ttaaaaaaac tttgtagtat cttttttggc gtgtgtttta tggaatagta tacaatgcaa 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcagtttatg accgcaattt ttatttcttc gcacgtctgg gcctctcatg acgtcaaatc 3960

3901 agtcaaatac tggcgttaaa aataaagaag cgtgcagacc cggagagtac tgcagtttag 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atgctcatcg tgaaaaagtt ttggagtatt tttggaattt ttcaatcaag tgaaagttta 4020

3961 tacgagtagc actttttcaa aacctcataa aaaccttaaa aagttagttc actttcaaat 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgaaattaat tttcctgctt ttgctttttg gggtttcccc tattgtttgt caagatttcg 4080

4021 actttaatta aaaggacgaa aacgaaaaac cccaaagggg ataacaaaca gttctaaagc 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aggacggcgt ttttcttgct aaaatcacaa gtattgatga gcacgatgca agaaagatcg 4140

4081 tcctgccgca aaaagaacga ttttagtgtt cataactact cgtgctacgt tctttctagc 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gaagaaggtt tgggtttgag gctcagtgga aggtgagtag aagttgataa tttgaaagtg 4200

4141 cttcttccaa acccaaactc cgagtcacct tccactcatc ttcaactatt aaactttcac 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gagtagtgtc tatggggttt ttgccttaaa tgacagaata cattcccaat ataccaaaca 4260

4201 ctcatcacag ataccccaaa aacggaattt actgtcttat gtaagggtta tatggtttgt 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ApaI(4292) .......... ..........

4261 taactgtttc ctactagtcg gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac 4320

4261 attgacaaag gatgatcagc cggcatgccc gggaaagcag agcgcgcaaa gccactactg 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 4380

4321 ccacttttgg agactgtgta cgtcgagggc ctctgccagt gtcgaacaga cattcgccta 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg 4440

4381 cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac aaccgcccac agccccgacc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata 4500

4441 gaattgatac gccgtagtct cgtctaacat gactctcacg tggtatacgc cacactttat 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccgcacagat gcgtaaggag aaaataccgc atcaggcggc cttaagggcc tcgtgatacg 4560

4501 ggcgtgtcta cgcattcctc ttttatggcg tagtccgccg gaattcccgg agcactatgc 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt 4620

4561 ggataaaaat atccaattac agtactatta ttaccaaaga atctgcagtc caccgtgaaa 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 4680

4621 agccccttta cacgcgcctt ggggataaac aaataaaaag atttatgtaa gtttatacat 4680

.......... .......... .......... .......... ......... Amp_promoter

.......... .......... .......... .......... .......... ..........

4681 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat 4740

4681 aggcgagtac tctgttattg ggactattta cgaagttatt ataacttttt ccttctcata 4740

Amp_promoter ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt 4800

4741 ctcataagtt gtaaaggcac agcgggaata agggaaaaaa cgccgtaaaa cggaaggaca 4800

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg 4860

4801 aaaacgagtg ggtctttgcg accactttca ttttctacga cttctagtca acccacgtgc 4860

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga 4920

4861 tcacccaatg tagcttgacc tagagttgtc gccattctag gaactctcaa aagcggggct 4920

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg 4980

4921 tcttgcaaaa ggttactact cgtgaaaatt tcaagacgat acaccgcgcc ataatagggc 4980

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt 5040

4981 ataactgcgg cccgttctcg ttgagccagc ggcgtatgtg ataagagtct tactgaacca 5040

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg 5100

5041 actcatgagt ggtcagtgtc ttttcgtaga atgcctaccg tactgtcatt ctcttaatac 5100

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg 5160

5101 gtcacgacgg tattggtact cactattgtg acgccggttg aatgaagact gttgctagcc 5160

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga 5220

5161 tcctggcttc ctcgattggc gaaaaaacgt gttgtacccc ctagtacatt gagcggaact 5220

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc 5280

5221 agcaaccctt ggcctcgact tacttcggta tggtttgctg ctcgcactgt ggtgctacgg 5280

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc 5340

5281 acatcgttac cgttgttgca acgcgtttga taattgaccg cttgatgaat gagatcgaag 5340

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc 5400

5341 ggccgttgtt aattatctga cctacctccg cctatttcaa cgtcctggtg aagacgcgag 5400

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg 5460

5401 ccgggaaggc cgaccgacca aataacgact atttagacct cggccactcg cacccagagc 5460

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac 5520

5461 gccatagtaa cgtcgtgacc ccggtctacc attcgggagg gcatagcatc aatagatgtg 5520

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc 5580

5521 ctgcccctca gtccgttgat acctacttgc tttatctgtc tagcgactct atccacggag 5580

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt 5640

5581 tgactaattc gtaaccattg acagtctggt tcaaatgagt atatatgaaa tctaactaaa 5640

Orf frame 2 Ampicillin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 5700

5641 ttttgaagta aaaattaaat tttcctagat ccacttctag gaaaaactat tagagtactg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 5760

5701 gttttaggga attgcactca aaagcaaggt gactcgcagt ctggggcatc ttttctagtt 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 5820

5761 tcctagaaga actctaggaa aaaaagacgc gcattagacg acgaacgttt gtttttttgg 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 5880

5821 tggcgatggt cgccaccaaa caaacggcct agttctcgat ggttgagaaa aaggcttcca 5880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 5940

5881 ttgaccgaag tcgtctcgcg tctatggttt atgacaggaa gatcacatcg gcatcaatcc 5940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 6000

5941 ggtggtgaag ttcttgagac atcgtggcgg atgtatggag cgagacgatt aggacaatgg 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 6060

6001 tcaccgacga cggtcaccgc tattcagcac agaatggccc aacctgagtt ctgctatcaa 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 6120

6061 tggcctattc cgcgtcgcca gcccgacttg ccccccaagc acgtgtgtcg ggtcgaacct 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gcgaacgacc tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct 6180

6121 cgcttgctgg atgtggcttg actctatgga tgtcgcactc gtaactcttt cgcggtgcga 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 6240

6181 agggcttccc tctttccgcc tgtccatagg ccattcgccg tcccagcctt gtcctctcgc 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 6300

6241 gtgctccctc gaaggtcccc ctttgcggac catagaaata tcaggacagc ccaaagcggt 6300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6301 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 6360

6301 ggagactgaa ctcgcagcta aaaacactac gagcagtccc cccgcctcgg ataccttttt 6360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6361 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 6420

6361 gcggtcgttg cgccggaaaa atgccaagga ccggaaaacg accggaaaac gagtgtacaa 6420

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 6480

6421 gaaaggacgc aataggggac taagacacct attggcataa tggcggaaac tcactcgact 6480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 6540

6481 atggcgagcg gcgtcggctt gctggctcgc gtcgctcagt cactcgctcc ttcgccttct 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 6600

6541 cgcgggttat gcgtttggcg gagaggggcg cgcaaccggc taagtaatta cgtcgaccgt 6600

.......... .......... .......... .......... .......... lacI.C

.......... .......... .......... .......... .......... ..........

6601 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 6660

6601 gctgtccaaa gggctgacct ttcgcccgtc actcgcgttg cgttaattac actcaatcga 6660

lacI.C .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 6720

6661 gtgagtaatc cgtggggtcc gaaatgtgaa atacgaaggc cgagcataca acacacctta 6720

.......... .......... ......... lac_promoter ......... ..........

.......... .......... .......... .......... .......... ..........

6721 tgtgagcgga taacaatttc acacaggaaa cagct..... .......... .......... 6755

6721 acactcgcct attgttaaag tgtgtccttt gtcga..... .......... .......... 6755

.......... .......... .......... .......... .......... ..........