l l LITMUS 28i Vector "Times New Roman lC IeAmpR_pro"Times New Roman lC ORF 2"Times New Roman lCɆ  Ampicillin"Times New Roman l  f1_origin"Times New Roman lC q pBR322_origin"Times New Roman lC . K lac_pro"Times New Roman lCɆ Y o M13_pUC_rev_pri"Times New Roman l n M13_reverse_pri"Times New Roman lC T7_pro"Times New Roman lC * < T7_pro"Times New Roman lCɆ @ P M13_forward20_pri"Times New Roman l I _ M13_pUC_fwd_pri"Times New Roman lC I lacZ_a"Times New Roman lHpaI"Times New Roman  lAseI"Times New Roman  lͻBglII(2470), BstBI(2476), XhoI(2502), EcoRI(2508), PstI(2518), EcoRV(2522), BamHI(2527), HindIII(2536), NcoI(2543), AatII(2554), AgeI(2556), XbaI(2562), SacI(2581), KpnI(2588), StuI(2601)"Times New Roman  vpj$j lPvuII"Times New Roman   ltest"Times New Romanz  P$@'U'3 ltest"Times New Roman    ~I