Sequence of LITMUS_38i

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Sequence of LITMUS_38i_Vector

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Sequence of LITMUS 38i Vector : 2814 BP; 734 A; 679 C; 713 G; 688 T; 0 other;

HpaI(3) .......... .......... .......... .......... ..........

1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 60

1 caattgatgc agtccaccgt gaaaagcccc tttacacgcg ccttggggat aaacaaataa 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 120

61 aaagatttat gtaagtttat acataggcga gtactctgtt attgggacta tttacgaagt 120

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 180

121 tattataact ttttccttct catactcata agttgtaaag gcacagcggg aataagggaa 180

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 240

181 aaaacgccgt aaaacggaag gacaaaaacg agtgggtctt tgcgaccact ttcattttct 240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 300

241 acgacttcta gtcaacccac gtgctcaccc aatgtagctt gacctagagt tgtcgccatt 300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct 360

301 ctaggaactc tcaaaagcgg ggcttcttgc aagaggttac tactcgtgaa aatttcaaga 360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 420

361 cgatacaccg cgccataata gggcacaact gcggcccgtt ctcgttgagc cagcggcgta 420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 480

421 tgtgataaga gtcttactga accaactcat gagtggtcag tgtcttttcg tagaatgcct 480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 540

481 accgtactgt cattctctta atacgtcacg acggtattgg tactcactat tgtgacgccg 540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 600

541 gttgaatgaa gactgttgct agcctcctgg cttcctcgat tggcgaaaaa acgtgttgta 600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 660

601 ccccctagta cattgagcgg aactagcaac ccttggcctc gacttacttc ggtatggttt 660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 720

661 gctgctcgca ctgtggtgct acggacatcg ttaccgttgt tgcaacgcgt ttgataattg 720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... AseI(755) .......... ..........

721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 780

721 accgcttgat gaatgagatc gaagggccgt tgttaattat ctgacctacc tccgcctatt 780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 840

781 tcaacgtcct ggtgaagacg cgagccggga aggccgaccg accaaataac gactatttag 840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 900

841 acctcggcca ctcgcaccca gagcgccata gtaacgtcgt gaccccggtc taccattcgg 900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 960

901 gagggcatag catcaataga tgtgctgccc ctcagtccgt tgatacctac ttgctttatc 960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1020

961 tgtctagcga ctctatccac ggagtgacta attcgtaacc attgacagtc tggttcaaat 1020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg 1080

1021 gagtatatat gaaatctaac taaatggggc caactattag tcttttcggg gtttttgtcc 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta 1140

1081 ttctaacata ttcgtttata aatttaacat ttgcaattat aaaacaattt taagcgcaat 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 1200

1141 ttaaaaacaa tttagtcgag taaaaaattg gttatccggc tttagccgtt ttagggaata 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca 1260

1201 tttagttttc ttatcgggct ctatcccaac tcacaacaag gtcaaacctt gttctcaggt 1260

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 1320

1261 gataatttct tgcacctgag gttgcagttt cccgcttttt ggcagatagt cccgctaccg 1320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta 1380

1321 ggtgatgcac ttggtagtgg gtttagttca aaaaacccca gctccacggc atttcgtgat 1380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga 1440

1381 ttagccttgg gatttccctc gggggctaaa tctcgaactg cccctttcgc ttgcaccgct 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 1500

1441 ctttccttcc cttctttcgc tttcctcgcc cgcgatcccg cgaccgttca catcgccagt 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg 1560

1501 gcgacgcgca ttggtggtgt gggcggcgcg aattacgcgg cgatgtcccg cgcattttcc 1560

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 1620

1561 tagatccact tctaggaaaa actattagag tactggtttt agggaattgc actcaaaagc 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 1680

1621 aaggtgactc gcagtctggg gcatcttttc tagtttccta gaagaactct aggaaaaaaa 1680

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 1740

1681 gacgcgcatt agacgacgaa cgtttgtttt tttggtggcg atggtcgcca ccaaacaaac 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 1800

1741 ggcctagttc tcgatggttg agaaaaaggc ttccattgac cgaagtcgtc tcgcgtctat 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 1860

1801 ggtttatgac aagaagatca catcggcatc aatccggtgg tgaagttctt gagacatcgt 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 1920

1861 ggcggatgta tggagcgaga cgattaggac aatggtcacc gacgacggtc accgctattc 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 1980

1921 agcacagaat ggcccaacct gagttctgct atcaatggcc tattccgcgt cgccagcccg 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2040

1981 acttgccccc caagcacgtg tgtcgggtcg aacctcgctt gctggatgtg gcttgactct 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 2100

2041 atggatgtcg cactcgatac tctttcgcgg tgcgaagggc ttccctcttt ccgcctgtcc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 2160

2101 ataggccatt cgccgtccca gccttgtcct ctcgcgtgct ccctcgaagg tccccctttg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2220

2161 cggaccatag aaatatcagg acagcccaaa gcggtggaga ctgaactcgc agctaaaaac 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 2280

2221 actacgagca gtccccccgc ctcggatacc tttttgcggt cgttgcgccg gaaaaatgcc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc 2340

2281 aaggaccgga aaacgaccgg aaaacgagtg tacattacac tcaatcgagt gagtaatccg 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 2400

2341 tggggtccga aatgtgaaat acgaaggccg agcatacaac acaccttaac actcgcctat 2400

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca 2460

2401 tgttaaagtg tgtcctttgt cgatactggt actaatgcgg ttcgatgcat tatgctgagt 2460

M13_pUC_rev_pri . M13_reverse_pri ....... .......... T7_pro

.......... ApaI(2471) ... NarI(2492),HindIII(2497) ... ..........

2461 ctataggggc ccgtgcaatt gaagccggct ggcgccaagc ttctctgcag gatatctgga 2520

2461 gatatccccg ggcacgttaa cttcggccga ccgcggttcg aagagacgtc ctatagacct 2520

.......... .......... .......... .......... .......... ..........

EcoRI(2526) NheI(2532) EagI(2540) ...... .......... ..........

2521 tccacgaatt cgctagcttc ggccgtgacg cgtctccgga tgtacaggca tgcgtcgacc 2580

2521 aggtgcttaa gcgatcgaag ccggcactgc gcagaggcct acatgtccgt acgcagctgg 2580

.......... .......... .......... .......... .......... ..........

.......... StuI(2592) .......... .......... .......... ..........

2581 ctctagtcaa ggcctatagt gagtcgtatt acggactggc cgtcgtttta caacgtcgtg 2640

2581 gagatcagtt ccggatatca ctcagcataa tgcctgaccg gcagcaaaat gttgcagcac 2640

.......... .......... T7_pro M13_forward20_pri M13_pUC_fwd_pri lacZ_a

.......... .......... .......... .......... .......... ..........

2641 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca 2700

2641 tgaccctttt gggaccgcaa tgggttgaat tagcggaacg tcgtgtaggg ggaaagcggt 2700

.......... .......... lacZ_a .......... .......... ..........

PvuII(2701) ........ .......... .......... .......... ..........

2701 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga 2760

2701 cgaccgcatt atcgcttctc cgggcgtggc tagcgggaag ggttgtcaac gcgtcggact 2760

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atggcgaatg gcgcttcgct tggtaataaa gcccgcttcg gcgggctttt tttt...... 2814

2761 taccgcttac cgcgaagcga accattattt cgggcgaagc cgcccgaaaa aaaa...... 2814

.......... .......... .......... .......... .......... ..........