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Gene/insert name: NANOG - RNAi
Alternative names: Nanog
Insert size (bp): 55
Gene/insert aliases: NANOG
Species of gene(s): H. sapiens (human)
Vector backbone: pLL3.7 (Search Vector Database)
Type of vector: Mammalian expression,Lentiviral,RNAi,Cre/Lox
Backbone size (bp): 7650
Cloning site 5': HpaI
Site destroyed during cloning: Yes
Cloning site 3': XhoI
Site destroyed during cloning: No
5' Sequencing primer: n/a (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: George Q Daley
Comments: Target sequence: GGGTTAAGCTGTAACATACTT (NM_024865: bp 1857-1877)
Article: High-efficiency RNA interference in human embryonic stem cells. Zaehres H et al. (Stem Cells. 2005 Mar . 23(3):299-305. Pubmed)