Sequence of LL-hNANOGi

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Sequence of LL-hNANOGi

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Sequence of LL-hNANOGi : 7690 BP; 2044 A; 1865 C; 1922 G; 1859 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 60

1 cagctgccta gccctctaga gggctagggg ataccacgtg agagtcatgt tagacgagac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 120

61 tacggcgtat caattcggtc atagacgagg gacgaacaca caacctccag cgactcatca 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 180

121 cgcgctcgtt ttaaattcga tgttgttccg ttccgaactg gctgttaacg tacttcttag 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 240

181 acgaatccca atccgcaaaa cgcgacgaag cgctacatgc ccggtctata tgcgcaactg 240

.......... .......... .......... .......... .......... ..........

.......... SpeI(252) .......... .......... .......... ..........

241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 300

241 taactaataa ctgatcaata attatcatta gttaatgccc cagtaatcaa gtatcgggta 300

.......... .......... CMV_im_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 360

301 tatacctcaa ggcgcaatgt attgaatgcc atttaccggg cggaccgact ggcgggttgc 360

.......... .......... CMV_im_pro CAG_enh .......... ..........

.......... .......... .......... .......... .......... ..........

361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 420

361 tgggggcggg taactgcagt tattactgca tacaagggta tcattgcggt tatccctgaa 420

.......... ...... CAG_enh CMV_im_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 480

421 aggtaactgc agttacccac ctcataaatg ccatttgacg ggtgaaccgt catgtagttc 480

.......... ...... CAG_enh CMV_im_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 540

481 acatagtata cggttcatgc gggggataac tgcagttact gccatttacc gggcggaccg 540

.......... ...... CAG_enh CMV_im_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 600

541 taatacgggt catgtactgg aataccctga aaggatgaac cgtcatgtag atgcataatc 600

.......... ...... CAG_enh CMV_im_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 660

601 agtagcgata atggtaccac tacgccaaaa ccgtcatgta gttacccgca cctatcgcca 660

.......... .......... CMV_im_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 720

661 aactgagtgc ccctaaaggt tcagaggtgg ggtaactgca gttaccctca aacaaaaccg 720

.......... .......... CMV_im_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 780

721 tggttttagt tgccctgaaa ggttttacag cattgttgag gcggggtaac tgcgtttacc 780

.......... .......... CMV_im_pro .......... .......... CMV_f_pri

.......... .......... .......... .......... .......... ..........

781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 840

781 cgccatccgc acatgccacc ctccagatat attcgtcgcg caaaacggac atgacccaga 840

CMV_f_pri CMV_im_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 900

841 gagaccaatc tggtctagac tcggaccctc gagagaccga ttgatccctt gggtgacgaa 900

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 960

901 ttcggagtta tttcgaacgg aactcacgaa gttcatcaca cacgggcaga caacacactg 960

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... NarI(1019)

961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1020

961 agaccattga tctctaggga gtctgggaaa atcagtcaca ccttttagag atcgtcaccg 1020

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1080

1021 cgggcttgtc cctgaacttt cgctttccct ttggtctcct cgagagagct gcgtcctgag 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1140

1081 ccgaacgact tcgcgcgtgc cgttctccgc tccccgccgc tgaccactca tgcggttttt 1140

.......... .......... .......... .......... ........ HIV-1_psi_pack

.......... .......... .......... .......... .......... ..........

1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1200

1141 aaaactgatc gcctccgatc ttcctctctc tacccacgct ctcgcagtca taattcgccc 1200

........ HIV-1_psi_pack ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1260

1201 cctcttaatc tagcgctacc cttttttaag ccaattccgg tccccctttc ttttttatat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1320

1261 ttaattttgt atatcatacc cgttcgtccc tcgatcttgc taagcgtcaa ttaggaccgg 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1380

1321 acaatctttg tagtcttccg acatctgttt atgaccctgt cgatgttggt agggaagtct 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1440

1381 gtcctagtct tcttgaatct agtaatatat tatgtcatcg ttgggagata acacacgtag 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1500

1441 tttcctatct ctattttctg tggttccttc gaaatctgtt ctatctcctt ctcgttttgt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga 1560

1501 tttcattctg gtggcgtgtc gttcgccggc cggcgcgact agaagtctgg acctcctcct 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca 1620

1561 ctatactccc tgttaacctc ttcacttaat atatttatat ttcatcattt ttaacttggt 1620

.......... .......... .......... ORF1 .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg 1680

1621 aatcctcatc gtgggtggtt ccgtttctct tctcaccacg tctctctttt ttctcgtcac 1680

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg 1740

1681 ccttatcctc gaaacaagga acccaagaac cctcgtcgtc cttcgtgata cccgcgtcgc 1740

.......... .......... ORF1 RRE .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac 1800

1741 agttactgcg actgccatgt ccggtctgtt aataacagac catatcacgt cgtcgtcttg 1800

.......... .......... ORF1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc 1860

1801 ttaaacgact cccgataact ccgcgttgtc gtagacaacg ttgagtgtca gaccccgtag 1860

.......... .......... ORF1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg 1920

1861 ttcgtcgagg tccgttctta ggaccgacac ctttctatgg atttcctagt tgtcgaggac 1920

.......... .......... ORF1 RRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt 1980

1921 ccctaaaccc caacgagacc ttttgagtaa acgtggtgac gacacggaac cttacgatca 1980

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga 2040

1981 acctcattat ttagagacct tgtctaaacc ttagtgtgct ggacctacct caccctgtct 2040

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa 2100

2041 ctttaattgt taatgtgttc gaattatgtg aggaattaac ttcttagcgt tttggtcgtt 2100

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt 2160

2101 cttttcttac ttgttcttaa taaccttaat ctatttaccc gttcaaacac cttaaccaaa 2160

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta 2220

2161 ttgtattgtt taaccgacac catatatttt aataagtatt actatcatcc tccgaaccat 2220

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca 2280

2221 ccaaattctt atcaaaaacg acatgaaaga tatcacttat ctcaatccgt ccctataagt 2280

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata 2340

2281 ggtaatagca aagtctgggt ggagggttgg ggctcccctg ggctgtccgg gcttccttat 2340

.......... .......... ORF1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca 2400

2341 cttcttcttc cacctctctc tctgtctctg tctaggtaag ctaatcactt gcctagccgt 2400

.......... .......... ORF1 .......... .......... ..........

.......... PstI(2420) .......... .......... .......... ..........

2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg 2460

2401 gacgcacgcg gttaagacgt ctgtttaccg tcataagtag gtgttaaaat tttcttttcc 2460

.......... .......... ORF1 .......... .......... cPPT

.......... .......... .......... .......... .......... ..........

2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca 2520

2461 cccctaaccc cccatgtcac gtcccctttc ttatcatctg tattatcgtt gtctgtatgt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga 2580

2521 ttgatttctt aatgtttttg tttaatgttt ttaagtttta aaagcccaaa taatgtccct 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .... ApaI(2612) XbaI(2616) .... ..........

2581 cagcagagat ccagtttggt tagtaccggg cccgctctag agatccgacg ccgccatctc 2640

2581 gtcgtctcta ggtcaaacca atcatggccc gggcgagatc tctaggctgc ggcggtagag 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 taggcccgcg ccggccccct cgcacagact tgtgggagaa gctcggctac tcccctgccc 2700

2641 atccgggcgc ggccggggga gcgtgtctga acaccctctt cgagccgatg aggggacggg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 cggttaattt gcatataata tttcctagta actatagagg cttaatgtgc gataaaagac 2760

2701 gccaattaaa cgtatattat aaaggatcat tgatatctcc gaattacacg ctattttctg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agataatctg ttctttttaa tactagctac attttacatg ataggcttgg atttctataa 2820

2761 tctattagac aagaaaaatt atgatcgatg taaaatgtac tatccgaacc taaagatatt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gagatacaaa tactaaatta ttattttaaa aaacagcaca aaaggaaact caccctaact 2880

2821 ctctatgttt atgatttaat aataaaattt tttgtcgtgt tttcctttga gtgggattga 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtaaagtaat tgtgtgtttt gagactataa atatcccttg gagaaaagcc ttgtttgggt 2940

2881 catttcatta acacacaaaa ctctgatatt tatagggaac ctcttttcgg aacaaaccca 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... XhoI(2990) ..........

2941 taagctgtaa catacttcaa gagagtatgt tacagcttaa cccttttttc tcgaggtcga 3000

2941 attcgacatt gtatgaagtt ctctcataca atgtcgaatt gggaaaaaag agctccagct 3000

.......... .......... .......... .......... ........ pBluescrKS_pri

.......... .......... .......... .......... .......... ..........

3001 cggtatcgat aagctcgctt cacgagattc cagcaggtcg agggacctaa taacttcgta 3060

3001 gccatagcta ttcgagcgaa gtgctctaag gtcgtccagc tccctggatt attgaagcat 3060

.......... .......... .......... .......... .......... loxP

.......... .......... .......... .......... NotI(3105) ..........

3061 tagcatacat tatacgaagt tatattaagg gttccaagct taagcggccg cgtggataac 3120

3061 atcgtatgta atatgcttca atataattcc caaggttcga attcgccggc gcacctattg 3120

.......... loxP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat tagttcatag 3180

3121 gcataatggc ggtacgtaat caataattat cattagttaa tgccccagta atcaagtatc 3180

.......... .......... .......... ......... CMV_imm_pro .........

.......... .......... .......... .......... .......... ..........

3181 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 3240

3181 gggtatatac ctcaaggcgc aatgtattga atgccattta ccgggcggac cgactggcgg 3240

.......... ......... CMV_imm_pro ......... CAG_enh ..........

.......... .......... .......... .......... .......... ..........

3241 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 3300

3241 gttgctgggg gcgggtaact gcagttatta ctgcatacaa gggtatcatt gcggttatcc 3300

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 3360

3301 ctgaaaggta actgcagtta cccacctcat aaatgccatt tgacgggtga accgtcatgt 3360

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 3420

3361 agttcacata gtatacggtt catgcggggg ataactgcag ttactgccat ttaccgggcg 3420

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 3480

3421 gaccgtaata cgggtcatgt actggaatac cctgaaagga tgaaccgtca tgtagatgca 3480

.......... ..... CMV_imm_pro CAG_enh ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 3540

3481 taatcagtag cgataatggt accactacgc caaaaccgtc atgtagttac ccgcacctat 3540

CAG_enh ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 3600

3541 cgccaaactg agtgccccta aaggttcaga ggtggggtaa ctgcagttac cctcaaacaa 3600

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 3660

3601 aaccgtggtt ttagttgccc tgaaaggttt tacagcattg ttgaggcggg gtaactgcgt 3660

.......... ......... CMV_imm_pro ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccg 3720

3661 ttacccgcca tccgcacatg ccaccctcca gatatattcg tctcgaccaa atcacttggc 3720

CMV_f_pri CMV_imm_pro ........ CMV_pro .......... ..........

NheI(3729) AgeI(3738) .......... .......... .......... ..........

3721 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gctgttcacc 3780

3721 agtctaggcg atcgcgatgg ccagcggtgg taccactcgt tcccgctcct cgacaagtgg 3780

.......... .......... .......... ORF1 EGFP ORF1 ..........

.......... .......... .......... .......... .......... ..........

3781 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg 3840

3781 ccccaccacg ggtaggacca gctcgacctg ccgctgcatt tgccggtgtt caagtcgcac 3840

.......... . ORF1 EGFP ORF1 EGFP_N_primer . .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc 3900

3841 aggccgctcc cgctcccgct acggtggatg ccgttcgact gggacttcaa gtagacgtgg 3900

.......... ........ ORF1 EGFP ORF1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag 3960

3901 tggccgttcg acgggcacgg gaccgggtgg gagcactggt gggactggat gccgcacgtc 3960

.......... ........ ORF1 EGFP ORF1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc 4020

3961 acgaagtcgg cgatggggct ggtgtacttc gtcgtgctga agaagttcag gcggtacggg 4020

.......... ........ ORF1 EGFP ORF1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc 4080

4021 cttccgatgc aggtcctcgc gtggtagaag aagttcctgc tgccgttgat gttctgggcg 4080

.......... ........ ORF1 EGFP ORF1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac 4140

4081 cggctccact tcaagctccc gctgtgggac cacttggcgt agctcgactt cccgtagctg 4140

.......... ........ ORF1 EGFP ORF1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac 4200

4141 aagttcctcc tgccgttgta ggaccccgtg ttcgacctca tgttgatgtt gtcggtgttg 4200

.......... ........ ORF1 EGFP ORF1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac 4260

4201 cagatatagt accggctgtt cgtcttcttg ccgtagttcc acttgaagtt ctaggcggtg 4260

.......... ........ ORF1 EGFP ORF1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc 4320

4261 ttgtagctcc tgccgtcgca cgtcgagcgg ctggtgatgg tcgtcttgtg ggggtagccg 4320

.......... ........ ORF1 EGFP ORF1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa 4380

4321 ctgccggggc acgacgacgg gctgttggtg atggactcgt gggtcaggcg ggactcgttt 4380

.......... ........ ORF1 EGFP ORF1 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc 4440

4381 ctggggttgc tcttcgcgct agtgtaccag gacgacctca agcactggcg gcggccctag 4440

.......... ........ ORF1 EGFP ORF1 EGFP_C_pri ........ ..........

.......... .......... ......... EcoRI(4471) ......... ..........

4441 actctcggca tggacgagct gtacaagtag gaattcgtcg agggacctaa taacttcgta 4500

4441 tgagagccgt acctgctcga catgttcatc cttaagcagc tccctggatt attgaagcat 4500

.......... ORF1 ORF1 EGFP .......... .......... loxP

.......... .......... .......... .......... .......... ..........

4501 tagcatacat tatacgaagt tatacatgtt taagggttcc ggttccacta ggtacaattc 4560

4501 atcgtatgta atatgcttca atatgtacaa attcccaagg ccaaggtgat ccatgttaag 4560

.......... loxP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt 4620

4561 ctatagttcg aatagctatt agttggagac ctaatgtttt aaacactttc taactgacca 4620

.......... .......... .......... WPRE .......... ..........

.......... .......... .......... .......... .......... ..........

4621 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 4680

4621 taagaattga tacaacgagg aaaatgcgat acacctatgc gacgaaatta cggaaacata 4680

.......... .......... WPRE .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 4740

4681 gtacgataac gaagggcata ccgaaagtaa aagaggagga acatatttag gaccaacgac 4740

.......... .......... WPRE ORF2 .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 4800

4741 agagaaatac tcctcaacac cgggcaacag tccgttgcac cgcaccacac gtgacacaaa 4800

.......... .......... WPRE ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 4860

4801 cgactgcgtt gggggtgacc aaccccgtaa cggtggtgga cagtcgagga aaggccctga 4860

.......... .......... WPRE ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 4920

4861 aagcgaaagg gggagggata acggtgccgc cttgagtagc ggcggacgga acgggcgacg 4920

.......... .......... WPRE ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg 4980

4921 acctgtcccc gagccgacaa cccgtgactg ttaaggcacc acaacagccc ctttagtagc 4980

.......... .......... WPRE ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5040

4981 aggaaaggaa ccgacgagcg gacacaacgg tggacctaag acgcgccctg caggaagacg 5040

.......... .......... WPRE ORF2 .......... .......... ..........

.......... .......... .......... ......... SacII(5082) .........

5041 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5100

5041 atgcagggaa gccgggagtt aggtcgcctg gaaggaaggg cgccggacga cggccgagac 5100

.......... .......... WPRE ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc 5160

5101 gccggagaag gcgcagaagc ggaagcggga gtctgctcag cctagaggga aacccggcgg 5160

.......... .......... WPRE ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tccccgcatc gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag 5220

5161 aggggcgtag ctatggcagc tggagctagc tctggatctt tttgtacctc gttagtgttc 5220

......... pBlueSKS_pri ORF2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga 5280

5221 atcgttatgt cgtcgatggt tacgactaac acggaccgat cttcgtgttc tcctcctcct 5280

.......... .......... ORF2 .......... .......... ..........

.......... .......... KpnI(5309) .......... ......... PvuII(5336)

5281 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt 5340

5281 ccacccaaaa ggtcagtgtg gagtccatgg aaattctggt tactgaatgt tccgtcgaca 5340

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 5400

5341 tctagaatcg gtgaaaaatt ttcttttccc ccctgacctt cccgattaag tgagggttgc 5400

.......... ....... ORF2 cPPT U3PPT ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca 5460

5401 ttctgttcta taggaactag acacctagat ggtgtgtgtt ccgatgaagg gactaaccgt 5460

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct 5520

5461 cttgatgtgt ggtcccggtc cctagtctat aggtgactgg aaacctacca cgatgttcga 5520

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt 5580

5521 tcatggtcaa ctcgttctct tccatcttct tcggttactt cctctcttgt gggcgaacaa 5580

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt 5640

5581 tgtgggacac tcggacgtac cctacctact gggcctctct cttcataatc tcacctccaa 5640

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc 5700

5641 actgtcggcg gatcgtaaag tagtgtaccg ggctctcgac gtaggcctga catgacccag 5700

.......... .......... ORF2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 5760

5701 agagaccaat ctggtctaga ctcggaccct cgagagaccg attgatccct tgggtgacga 5760

......... ORF2 HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 5820

5761 attcggagtt atttcgaacg gaactcacga agttcatcac acacgggcag acaacacact 5820

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat 5880

5821 gagaccattg atctctaggg agtctgggaa aatcagtcac accttttaga gatcgtcgta 5880

.......... ......... HIV truncHIV ......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 5940

5881 cactcgtttt ccggtcgttt tccggtcctt ggcatttttc cggcgcaacg accgcaaaaa 5940

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

5941 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 6000

5941 ggtatccgag gcggggggac tgctcgtagt gtttttagct gcgagttcag tctccaccgc 6000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 6060

6001 tttgggctgt cctgatattt ctatggtccg caaaggggga ccttcgaggg agcacgcgag 6060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 6120

6061 aggacaaggc tgggacggcg aatggcctat ggacaggcgg aaagagggaa gcccttcgca 6120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 6180

6121 ccgcgaaaga gtatcgagtg cgacatccat agagtcaagc cacatccagc aagcgaggtt 6180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 6240

6181 cgacccgaca cacgtgcttg gggggcaagt cgggctggcg acgcggaata ggccattgat 6240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 6300

6241 agcagaactc aggttgggcc attctgtgct gaatagcggt gaccgtcgtc ggtgaccatt 6300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6301 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 6360

6301 gtcctaatcg tctcgctcca tacatccgcc acgatgtctc aagaacttca ccaccggatt 6360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 6420

6361 gatgccgatg tgatcttctt gtcataaacc atagacgcga gacgacttcg gtcaatggaa 6420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 6480

6421 gcctttttct caaccatcga gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa 6480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 6540

6481 aaaacaaacg ttcgtcgtct aatgcgcgtc tttttttcct agagttcttc taggaaacta 6540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

6541 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 6600

6541 gaaaagatgc cccagactgc gagtcacctt gcttttgagt gcaattccct aaaaccagta 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 6660

6601 ctctaatagt ttttcctaga agtggatcta ggaaaattta atttttactt caaaatttag 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 6720

6661 ttagatttca tatatactca tttgaaccag actgtcaatg gttacgaatt agtcactccg 6720

.......... .......... .......... ....... ORF1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

6721 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 6780

6721 tggatagagt cgctagacag ataaagcaag taggtatcaa cggactgagg ggcagcacat 6780

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 6840

6781 ctattgatgc tatgccctcc cgaatggtag accggggtca cgacgttact atggcgctct 6840

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 6900

6841 gggtgcgagt ggccgaggtc taaatagtcg ttatttggtc ggtcggcctt cccggctcgc 6900

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 6960

6901 gtcttcacca ggacgttgaa ataggcggag gtaggtcaga taattaacaa cggcccttcg 6960

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... FspI(6992) .......... ..........

6961 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 7020

6961 atctcattca tcaagcggtc aattatcaaa cgcgttgcaa caacggtaac gatgtccgta 7020

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 7080

7021 gcaccacagt gcgagcagca aaccataccg aagtaagtcg aggccaaggg ttgctagttc 7080

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 7140

7081 cgctcaatgt actagggggt acaacacgtt ttttcgccaa tcgaggaagc caggaggcta 7140

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 7200

7141 gcaacagtct tcattcaacc ggcgtcacaa tagtgagtac caataccgtc gtgacgtatt 7200

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 7260

7201 aagagaatga cagtacggta ggcattctac gaaaagacac tgaccactca tgagttggtt 7260

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 7320

7261 cagtaagact cttatcacat acgccgctgg ctcaacgaga acgggccgca gttatgccct 7320

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 7380

7321 attatggcgc ggtgtatcgt cttgaaattt tcacgagtag taaccttttg caagaagccc 7380

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 7440

7381 cgcttttgag agttcctaga atggcgacaa ctctaggtca agctacattg ggtgagcacg 7440

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 7500

7441 tgggttgact agaagtcgta gaaaatgaaa gtggtcgcaa agacccactc gtttttgtcc 7500

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 7560

7501 ttccgtttta cggcgttttt tcccttattc ccgctgtgcc tttacaactt atgagtatga 7560

.......... ....... ORF1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 7620

7561 gaaggaaaaa gttataataa cttcgtaaat agtcccaata acagagtact cgcctatgta 7620

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

7621 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 7680

7621 taaacttaca taaatctttt tatttgttta tccccaaggc gcgtgtaaag gggcttttca 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 gccacctgac .......... .......... .......... .......... .......... 7690

7681 cggtggactg .......... .......... .......... .......... .......... 7690

.......... .......... .......... .......... .......... ..........