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    Plasmid map of LV-Cre-SD

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    LV-Cre-SD
    Gene/insert name: nlsCre
    Alternative names: nls Cre LV-Cre cre
    Insert size (bp): 1867
    Gene/insert aliases: cre
    Species of gene(s): Enterobacteriophage P1
    Relevant mutations/deletions: loxP site introduced downstream of nlsCre
    Vector backbone: n/a (Search Vector Database)
    Type of vector: Mammalian expression,Cre/Lox
    Backbone size (bp): 7766
    Cloning site 5': EcoRI
    Site destroyed during cloning: No
    Cloning site 3': KpnI
    Site destroyed during cloning: No
    5' Sequencing primer: n/a (List of Sequencing Primers)
    Bacteria resistance: Ampicillin
    High or low copy: Low Copy
    Grow in standard E. coli @ 37C: Yes
    Selectable markers: none
    If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: The cre is from pMC-Cre (Gu et al. 1993 Cell 73, 1155-1164
    Plasmid Provided In: Stbl3
    Principal Investigator: Inder M Verma
    Comments: LV-Cre-SD minimizes the cytotoxic effects of Cre by causing Cre to be recombined out after transient expression. See article for details.
    Article: Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Pfeifer A et al. (Proc Natl Acad Sci U S A. 2001 Sep 25. 98(20):11450-5. Pubmed)



    image of LV-Cre-SD



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