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LV-Cre-SD
Gene/insert name: nlsCre
Alternative names: nls Cre LV-Cre cre
Insert size (bp): 1867
Gene/insert aliases: cre
Species of gene(s): Enterobacteriophage P1
Relevant mutations/deletions: loxP site introduced downstream of nlsCre
Vector backbone: n/a (Search Vector Database)
Type of vector: Mammalian expression,Cre/Lox
Backbone size (bp): 7766
Cloning site 5': EcoRI
Site destroyed during cloning: No
Cloning site 3': KpnI
Site destroyed during cloning: No
5' Sequencing primer: n/a (List of Sequencing Primers)
Bacteria resistance: Ampicillin
High or low copy: Low Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: none
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: The cre is from pMC-Cre (Gu et al. 1993 Cell 73, 1155-1164
Plasmid Provided In: Stbl3
Principal Investigator: Inder M Verma
Comments: LV-Cre-SD minimizes the cytotoxic effects of Cre by causing Cre to be recombined out after transient expression. See article for details.
Article: Delivery of the Cre recombinase by a self-deleting lentiviral vector: efficient gene targeting in vivo. Pfeifer A et al. (Proc Natl Acad Sci U S A. 2001 Sep 25. 98(20):11450-5. Pubmed)