Sequence of Lamp1-RFP

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Sequence of Lamp1-RFP

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Sequence of Lamp1-RFP : 6257 BP; 1452 A; 1640 C; 1544 G; 1339 T; 282 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... .... CMV_immearly_promoter .... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ... CMV_immearly_promoter CAG_enhancer ... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

........ CAG_enhancer CMV_immearly_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... .... CMV_immearly_promoter .... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

......... CMV_immearly_promoter CMV_fwd_primer CMV_promoter .

.......... .......... .......... .......... .......... ..........

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

... CMV_immearly_promoter CMV_promoter ... .......... ..........

BglII,XhoI SacI,HindIII(622) EcoRI(629) SmaI(653)

601 ccggactcag atctcgagct caagcttcga attctcgcca ccatggcggc cccgggcgcc 660

601 ggcctgagtc tagagctcga gttcgaagct taagagcggt ggtaccgccg gggcccgcgg 660

.......... .......... .......... .......... ......... Orf frame 1

.......... .......... .......... .......... .......... ..........

661 cggcggccgc tgctcctgtt gctgctggca ggccttgcac acagcgcccc agcactgttc 720

661 gccgccggcg acgaggacaa cgacgaccgt ccggaacgtg tgtcgcgggg tcgtgacaag 720

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gaggtgaaag acaacaacgg cacagcgtgt ataatggcca gcttctctgc ctcctttctg 780

721 ctccactttc tgttgttgcc gtgtcgcaca tattaccggt cgaagagacg gaggaaagac 780

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 accacctatg atgctggaca tgtttctaag gtctcgaata tgaccctgcc agcctctgca 840

781 tggtggatac tacgacctgt acaaagattc cagagcttat actgggacgg tcggagacgt 840

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gaagtcctga agaatagcag ctcttgtggt gaaaagaatg cttctgagcc caccctcgca 900

841 cttcaggact tcttatcgtc gagaacacca cttttcttac gaagactcgg gtgggagcgt 900

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atcacctttg gagaaggata tttactgaaa ctcaccttca caaaaaacac aacacgttac 960

901 tagtggaaac ctcttcctat aaatgacttt gagtggaagt gttttttgtg ttgtgcaatg 960

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 agtgtccagc acatgtattt cacatataac ctgtcagaca cacaattctt tcccaatgcc 1020

961 tcacaggtcg tgtacataaa gtgtatattg gacagtctgt gtgttaagaa agggttacgg 1020

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agctccaaag ggcccgacac tgtggattcc acaactgaca tcaaggcaga catcaacaaa 1080

1021 tcgaggtttc ccgggctgtg acacctaagg tgttgactgt agttccgtct gtagttgttt 1080

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 acataccgat gtgtcagcga catcagggtc tacatgaaga atgtgaccat tgtgctctgg 1140

1081 tgtatggcta cacagtcgct gtagtcccag atgtacttct tacactggta acacgagacc 1140

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gacgctacta tccaggccta cctgccgagt agcaacttca gcaaggaaga gacacgctgc 1200

1141 ctgcgatgat aggtccggat ggacggctca tcgttgaagt cgttccttct ctgtgcgacg 1200

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ccacaggatc aaccttcccc aactactggg ccacccagcc cctcaccacc acttgtgccc 1260

1201 ggtgtcctag ttggaagggg ttgatgaccc ggtgggtcgg ggagtggtgg tgaacacggg 1260

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 acaaacccca gtgtgtccaa gtacaatgtg actggtgaca atggaacctg cctgctggcc 1320

1261 tgtttggggt cacacaggtt catgttacac tgaccactgt taccttggac ggacgaccgg 1320

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tctatggcac tgcaactcaa catcacctac atgaagaagg acaacacgac tgtgaccaga 1380

1321 agataccgtg acgttgagtt gtagtggatg tacttcttcc tgttgtgctg acactggtct 1380

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcattcaaca tcaacccaag tgacaaatat agtgggactt gcggtgccca gttggtgacc 1440

1381 cgtaagttgt agttgggttc actgtttata tcaccctgaa cgccacgggt caaccactgg 1440

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctgaaggtgg ggaacaagag cagagtcctg gagctgcagt ttgggatgaa tgccacttct 1500

1441 gacttccacc ccttgttctc gtctcaggac ctcgacgtca aaccctactt acggtgaaga 1500

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agcctgtttt tcctgcaagg agttcagttg aacatgactc ttcctgatgc catagagccc 1560

1501 tcggacaaaa aggacgttcc tcaagtcaac ttgtactgag aaggactacg gtatctcggg 1560

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 acgttcagca cctccaacta ttccctgaaa gctcttcagg ccagtgtcgg caactcatac 1620

1561 tgcaagtcgt ggaggttgat aagggacttt cgagaagtcc ggtcacagcc gttgagtatg 1620

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 aagtgcaaca gtgaggagca catctttgtc agcaaggcgc tcgccctcaa tgtcttcagc 1680

1621 ttcacgttgt cactcctcgt gtagaaacag tcgttccgcg agcgggagtt acagaagtcg 1680

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtgcaagtcc aggctttcag ggtagaaagt gacaggtttg ggtctgtgga agagtgtgta 1740

1681 cacgttcagg tccgaaagtc ccatctttca ctgtccaaac ccagacacct tctcacacat 1740

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 caggacggta acaacatgct gatccccatt gctgtgggcg gggccctggc agggctggtc 1800

1741 gtcctgccat tgttgtacga ctaggggtaa cgacacccgc cccgggaccg tcccgaccag 1800

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctcatcgtcc tcatcgccta cctcatcggc aggaagagga gtcacgcggg ctatcagacc 1860

1801 gagtagcagg agtagcggat ggagtagccg tccttctcct cagtgcgccc gatagtctgg 1860

.......... ......... Orf frame 1 ......... .......... ..........

BamHI(1866) AgeI(1872) ........ .......... .......... ..........

1861 atccgggatc caccggtcgc caccatggcc tcctccgagg acgtcatcaa ggagttcatg 1920

1861 taggccctag gtggccagcg gtggtaccgg aggaggctcc tgcagtagtt cctcaagtac 1920

.......... ......... Orf frame 1 ......... DsRed2 ..........

.......... .......... .......... .......... .......... ..........

1921 cgcttcaagg tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag 1980

1921 gcgaagttcc acgcgtacct cccgaggcac ttgccggtgc tcaagctcta gctcccgctc 1980

.......... ...... Orf frame 1 DsRed2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc 2040

1981 ccgctcccgg cggggatgct cccgtgggtc tggcggttcg acttccactg gttcccgccg 2040

.......... ...... Orf frame 1 DsRed2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cccctgccct tcgcctggga catcctgtcc cctcagttcc agtacggctc caaggcctac 2100

2041 ggggacggga agcggaccct gtaggacagg ggagtcaagg tcatgccgag gttccggatg 2100

.......... ...... Orf frame 1 DsRed2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag 2160

2101 cacttcgtgg ggcggctgta ggggctgatg aacttcgaca ggaaggggct cccgaagttc 2160

.......... ...... Orf frame 1 DsRed2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc 2220

2161 accctcgcgc actacttgaa gctcctgccg ccgcaccact ggcactgggt cctgaggagg 2220

.......... ...... Orf frame 1 DsRed2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac 2280

2221 gacgtcctgc cgctcaagta gatgttccac ttcgacgcgc cgtggttgaa ggggaggctg 2280

.......... ...... Orf frame 1 DsRed2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg gatgtacccc 2340

2281 ccggggcatt acgtcttctt ctggtacccg accctccgga ggtggctcgc ctacatgggg 2340

.......... ...... Orf frame 1 DsRed2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaggacggcg ccctgaaggg cgagatcaag atgaggctga agctgaagga cggcggccac 2400

2341 ctcctgccgc gggacttccc gctctagttc tactccgact tcgacttcct gccgccggtg 2400

.......... ...... Orf frame 1 DsRed2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tacgacgccg aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc 2460

2401 atgctgcggc tccagttctg gtggatgtac cggttcttcg ggcacgtcga cgggccgcgg 2460

.......... ...... Orf frame 1 DsRed2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tacaagaccg acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa 2520

2461 atgttctggc tgtagttcga cctgtagtgg agggtgttgc tcctgatgtg gtagcacctt 2520

........ Orf frame 1 DsRed2 dsRed1_C_primer ........ ..........

.......... .......... .......... .......... .......... ..........

2521 cagtacgagc gcgccgaggg ccgccactcc accggcgcct tgtacaagta aagcggccgc 2580

2521 gtcatgctcg cgcggctccc ggcggtgagg tggccgcgga acatgttcat ttcgccggcg 2580

DsRed2 ......... Orf frame 1 ......... .......... ..........

XbaI(2584) .......... .......... .......... .......... ..........

2581 gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc 2640

2581 ctgagatcta gtattagtcg gtatggtgta aacatctcca aaatgaacga aattttttgg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... HpaI(2693)

2641 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt 2700

2641 agggtgtgga gggggacttg gactttgtat tttacttacg ttaacaacaa caattgaaca 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 2760

2701 aataacgtcg aatattacca atgtttattt cgttatcgta gtgtttaaag tgtttatttc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... AflII(2812)

2761 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg 2820

2761 gtaaaaaaag tgacgtaaga tcaacaccaa acaggtttga gtagttacat agaattccgc 2820

.......... .......... ........ EBV_rev_primer ........ ..........

.......... .......... .......... .......... .......... ..........

2821 taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 2880

2821 atttaacatt cgcaattata aaacaatttt aagcgcaatt taaaaacaat ttagtcgagt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 2940

2881 aaaaaattgg ttatccggct ttagccgttt tagggaatat ttagttttct tatctggctc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 3000

2941 tatcccaact cacaacaagg tcaaaccttg ttctcaggtg ataatttctt gcacctgagg 3000

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

3001 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 3060

3001 ttgcagtttc ccgctttttg gcagatagtc ccgctaccgg gtgatgcact tggtagtggg 3060

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 3120

3061 attagttcaa aaaaccccag ctccacggca tttcgtgatt tagccttggg atttccctcg 3120

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 3180

3121 ggggctaaat ctcgaactgc ccctttcggc cgcttgcacc gctctttcct tcccttcttt 3180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 3240

3181 cgctttcctc gcccgcgatc ccgcgaccgt tcacatcgcc agtgcgacgc gcattggtgg 3240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat 3300

3241 tgtgggcggc gcgaattacg cggcgatgtc ccgcgcagtc caccgtgaaa agccccttta 3300

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 3360

3301 cacgcgcctt ggggataaac aaataaaaag atttatgtaa gtttatacat aggcgagtac 3360

.......... .......... .......... ........ AmpR_promoter ........

.......... .......... .......... .......... .......... ..........

3361 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa 3420

3361 tctgttattg ggactattta cgaagttatt ataacttttt ccttctcagg actccgcctt 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 3480

3421 tcttggtcga caccttacac acagtcaatc ccacaccttt caggggtccg aggggtcgtc 3480

.......... .... pBABE_3_primer SV40_enhancer SV40_promoter ....

.......... .......... .......... .......... .......... ..........

3481 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 3540

3481 cgtcttcata cgtttcgtac gtagagttaa tcagtcgttg gtccacacct ttcaggggtc 3540

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 3600

3541 cgaggggtcg tccgtcttca tacgtttcgt acgtagagtt aatcagtcgt tggtatcagg 3600

.......... . SV40_enhancer SV40_promoter . .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 3660

3601 gcggggattg aggcgggtag ggcggggatt gaggcgggtc aaggcgggta agaggcgggg 3660

...... SV40_enhancer SV40_promoter ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 3720

3661 taccgactga ttaaaaaaaa taaatacgtc tccggctccg gcggagccgg agactcgata 3720

.. SV40_origin SV40_promoter SV40pro_F .. .......... ..........

.......... .......... .......... .......... ClaI(3770) ..........

3721 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag 3780

3721 aggtcttcat cactcctccg aaaaaacctc cggatccgaa aacgtttcta gctagttctc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc 3840

3781 tgtcctactc ctagcaaagc gtactaactt gttctaccta acgtgcgtcc aagaggccgg 3840

.......... .......... .......... ..... Orf frame 1 Neomycin .....

.......... .......... .......... .......... .......... ..........

3841 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat 3900

3841 cgaacccacc tctccgataa gccgatactg acccgtgttg tctgttagcc gacgagacta 3900

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg 3960

3901 cggcggcaca aggccgacag tcgcgtcccc gcgggccaag aaaaacagtt ctggctggac 3960

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg 4020

3961 aggccacggg acttacttga cgttctgctc cgtcgcgccg atagcaccga ccggtgctgc 4020

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta 4080

4021 ccgcaaggaa cgcgtcgaca cgagctgcaa cagtgacttc gcccttccct gaccgacgat 4080

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta 4140

4081 aacccgcttc acggccccgt cctagaggac agtagagtgg aacgaggacg gctctttcat 4140

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc 4200

4141 aggtagtacc gactacgtta cgccgccgac gtatgcgaac taggccgatg gacgggtaag 4200

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc 4260

4201 ctggtggttc gctttgtagc gtagctcgct cgtgcatgag cctaccttcg gccagaacag 4260

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg 4320

4261 ctagtcctac tagacctgct tctcgtagtc cccgagcgcg gtcggcttga caagcggtcc 4320

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg 4380

4321 gagttccgct cgtacgggct gccgctccta gagcagcact gggtaccgct acggacgaac 4380

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt 4440

4381 ggcttatagt accacctttt accggcgaaa agacctaagt agctgacacc ggccgaccca 4440

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc 4500

4441 caccgcctgg cgatagtcct gtatcgcaac cgatgggcac tataacgact tctcgaaccg 4500

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc 4560

4501 ccgcttaccc gactggcgaa ggagcacgaa atgccatagc ggcgagggct aagcgtcgcg 4560

.......... ..... Orf frame 1 Neomycin ..... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga 4620

4561 tagcggaaga tagcggaaga actgctcaag aagactcgcc ctgagacccc aagctttact 4620

..... Orf frame 1 Neomycin ..... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg 4680

4621 ggctggttcg ctgcgggttg gacggtagtg ctctaaagct aaggtggcgg cggaagatac 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 4740

4681 tttccaaccc gaagccttag caaaaggccc tgcggccgac ctactaggag gtcgcgcccc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga 4800

4741 tagagtacga cctcaagaag cgggtgggat ccccctccga ttgactttgt gccttcctct 4800

.......... .......... .......... ....... TK_PA_terminator .......

.......... .......... .......... .......... .......... ..........

4801 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg 4860

4801 gttatggcct tccttgggcg cgatactgcc gttatttttc tgtcttattt tgcgtgccac 4860

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc 4920

4861 aacccagcaa acaagtattt gcgccccaag ccagggtccc gaccgtgaga cagctatggg 4920

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc 4980

4921 gtggctctgg ggtaaccccg gttatgcggg cgcaaagaag gaaaaggggt ggggtggggg 4980

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag 5040

4981 gttcaagccc acttccgggt cccgagcgtc ggttgcagcc ccgccgtccg ggacggtatc 5040

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 5100

5041 ggagtccaat gagtatatat gaaatctaac taaattttga agtaaaaatt aaattttcct 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5160

5101 agatccactt ctaggaaaaa ctattagagt actggtttta gggaattgca ctcaaaagca 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5220

5161 aggtgactcg cagtctgggg catcttttct agtttcctag aagaactcta ggaaaaaaag 5220

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

5221 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5280

5221 acgcgcatta gacgacgaac gtttgttttt ttggtggcga tggtcgccac caaacaaacg 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5340

5281 gcctagttct cgatggttga gaaaaaggct tccattgacc gaagtcgtct cgcgtctatg 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5400

5341 gtttatgaca ggaagatcac atcggcatca atccggtggt gaagttcttg agacatcgtg 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5460

5401 gcggatgtat ggagcgagac gattaggaca atggtcaccg acgacggtca ccgctattca 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 5520

5461 gcacagaatg gcccaacctg agttctgcta tcaatggcct attccgcgtc gccagcccga 5520

.......... ........ pBR322_origin ........ .......... ..........

......... ApaLI(5534) ......... .......... .......... ..........

5521 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 5580

5521 cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg ctggatgtgg cttgactcta 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 5640

5581 tggatgtcgc actcgatact ctttcgcggt gcgaagggct tccctctttc cgcctgtcca 5640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5641 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 5700

5641 taggccattc gccgtcccag ccttgtcctc tcgcgtgctc cctcgaaggt ccccctttgc 5700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 5760

5701 ggaccataga aatatcagga cagcccaaag cggtggagac tgaactcgca gctaaaaaca 5760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 5820

5761 ctacgagcag tccccccgcc tcggatacct ttttgcggtc gttgcgccgg aaaaatgcca 5820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 5880

5821 aggaccggaa aacgaccgga aaacgagtgt acaagaaagg acgcaatagg ggactaagac 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 tggataaccg tattaccgcc atgcathelp blastnblas tprogramsf indregions 5940

5881 acctattggc ataatggcgg tacgtadelp vltsanvlts apyocytksf inhyecions 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ofsimilari tybetweenb iologicals equencesbl astnsearch esncbisdna 6000

5941 ofsikiltyi arveaweenv iolocigtls eqaengesvl tsansetygd esngvishnt 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 databasefo rdnasequen cessimilar toyoursequ encehelpmo resequence 6060

6001 htatvtsefo yhntseqaen gessikilty aoroayseqa engedelpko yeseqaenge 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 optionsvie wthesequen ceindiffer entformats findrestri ctionsites 6120

6061 opaionsbie wadeseqaen geinhiffey enafoyktas finhyesayi gaionsiaes 6120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 viewopenre adingframe translatio nsandmoreh elpfultipc lickonthea 6180

6121 biewopenye thincfytke aytnsltaio nstnhkoyed elpfalaipg ligmonadet 6180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 nalyzesequ encebutton toseerestr ictionsite svieworfse tcaddgenes 6240

6181 ntlrzeseqa engevaaaon aoseeyesay igaionsiae sbiewoyfse agthhcenes 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 sequencing results... .......... .......... .......... .......... 6257

6241 seqaenginc yesalas... .......... .......... .......... .......... 6257

.......... .......... .......... .......... .......... ..........