|
pCMV-dR8.2 dvpr
Alternative names: pHR' CMV 8.2 deltaR , pCMV-dR8.2 dvpr, pCMVdR8.2 dvpr
Type of vector: Mammalian expression,Lentiviral
5' Sequencing primer: CMV Forward
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Bob Weinberg
Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA et al. (RNA 2003 Apr;9(4):493-501. Pubmed)
pLKO.1 puro
Alternative names: LKO.1
Insert size (bp): Unknown
Vector backbone: pLKO.1
Type of vector: Mammalian expression,Lentiviral,RNAi
Backbone size (bp): 7032
Cloning site 5': AgeI
Site destroyed during cloning: No
Cloning site 3': EcoRI
Site destroyed during cloning: No
5' Sequencing primer: LKO.1 5'
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: Puromycin
Plasmid Provided In: DH5a
Principal Investigator: Bob Weinberg
Comments: Empty lentiviral vector for siRNA expression; replaces Lentihair.
Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart SA et al. (RNA 2003 Apr;9(4):493-501. Pubmed)
pLionII
Vector backbone: pLionII
Backbone manufacturer: Nolan Lab
Type of vector: Lentiviral
Backbone size (bp): 7002
5' Sequencing primer: n/a
3' Sequencing primer: M13 reverse primer
Bacteria resistance: Ampicillin
High or low copy: Don't Know
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Garry Nolan
Comments: This is a transfer vector (slightly lower titer, highest expression level) from Nolan lab's FELIX vector system. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.
pTiger
Vector backbone: pTiger
Backbone manufacturer: Nolan Lab
Type of vector: Lentiviral
Backbone size (bp): 7164
5' Sequencing primer: n/a
3' Sequencing primer: M13 reverse primer
Bacteria resistance: Ampicillin
High or low copy: Don't Know
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: Garry Nolan
Comments: This is a transfer vector (highest titer) from Nolan lab's FELIX vector system. The FELIX vector system consists of three plasmids - structural, envelope, and transfer vector - which are cotransfected into 293T cells to produce virus. The structural construct makes Gag-Pol and Rev (some variants also make Vif and A). Any envelope construct can be used for pseudotyping - VSV-G tends to give the highest efficiency and broadest host range. The transfer vector contains the viral LTRs and packaging signal. Any expression cassette (promoter and gene of interest) can be cloned into the vector. Inserts can be up to 8.5kB.
|