Lentiviral Plasmids
EF.CMV.RFP to pCMV-dR8.2 dvpr | pLionII to pLVCT-tTR-KRAB | pMDLg_pRRE to pTiger |
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| Plasmids 0 - 4 | Descriptions |
 | EF.CMV.RFP - Gene/insert name: None; Vector backbone: EF.CMV.RFP (Search Vector Database); 5' Sequencing primer: CMV-F; Bacteria resistance: Ampicillin; High or low copy: Don't Know; Grow in standard E. coli @ 37C: Yes; Selectable markers: RFP; Plasmid Provided In: Stbl3; Principal Investigator: Linzhao Cheng; Terms and Licenses: MTA; Article: Lentiviral vectors with two independent internal... |
 | LL-hNANOGi - Gene/insert name: NANOG - RNAi; Alternative names: Nanog; Gene/insert aliases: NANOG; Species of gene(s): H. sapiens (human); Vector backbone: pLL3.7 (Search Vector Database); Cloning site 5': HpaI; Site destroyed during cloning: Yes; Cloning site 3': XhoI; Site destroyed during cloning: No; 5' Sequencing primer: n/a (List of Sequencing Primers); Bacteria resistance: Amp... |
 | LL-hOCT4i-1 - Gene/insert name: OCT4 - RNAi; Alternative names: Oct4; Gene/insert aliases: POU5F1, OCT3, OCT4, OTF3, OTF4, MGC22487; Species of gene(s): H. sapiens (human); Vector backbone: pLL3.7 (Search Vector Database); Cloning site 5': HpaI; Site destroyed during cloning: Yes; Cloning site 3': XhoI; Site destroyed during cloning: No; 5' Sequencing primer: n/a (List of Sequencing P... |
 | pCMV-dR8.2.dvpr - Gene/insert name: none; Alternative names: pHR' CMV 8.2 deltaR, pCMV-dR8.2 dvpr, pCMVdR8.2 dvpr; Vector backbone: na (Search Vector Database); 5' Sequencing primer: CMV Forward (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Plasmid Provided In: DH5a; Principal Investigator: Bob Weinberg; Comme... |
 | pCMV-dR8.2 dvpr - Alternative names: pHR' CMV 8.2 deltaR , pCMV-dR8.2 dvpr, pCMVdR8.2 dvpr ;
5' Sequencing primer: CMV Forward ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes;
Plasmid Provided In: DH5a ;
Principal Investigator: Bob Weinberg ;
Article: Lentivirus-delivered stable gene silencing by RNAi in primary cells. Stewart S... |
| Plasmids 5 - 9 | Descriptions |
 | pLionII - Vector backbone: pLionII;
Backbone manufacturer: Nolan Lab ;
5' Sequencing primer: n/a ;
3' Sequencing primer: M13 reverse primer ;
Bacteria resistance: Ampicillin ;
High or low copy: Don't Know ;
Grow in standard E. coli @ 37C: Yes;
Plasmid Provided In: DH5a ;
Principal Investigator: Garry Nolan ;
Comments: This is a transfer vector (slightly lower titer, hig... |
 | pLKO.1 puro - Alternative names: LKO.1 ;
Vector backbone: pLKO.1 ;
Cloning site 5': AgeI ;
Site destroyed during cloning: No ;
Cloning site 3': EcoRI ;
Site destroyed during cloning: No ;
5' Sequencing primer: LKO.1 5' ;
Bacteria resistance: Ampicillin ;
High or low copy: High Copy ;
Grow in standard E. coli @ 37C: Yes ;
Selectable markers: Puromycin ;
Plasmi... |
 | pLKO.1 - TRC cloning vector - Gene/insert name: stuffer; Vector backbone: pLKO.1 (Search Vector Database); Cloning site 5': AgeI; Site destroyed during cloning: No; Cloning site 3': EcoRI; Site destroyed during cloning: No; 5' Sequencing primer: LKO.1 5' (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 3... |
 | pLOVE empty vector - Gene/insert name: None; Vector backbone: pLOVE (for Lentiviral Over-Expression) (Search Vector Database); Cloning site 5': attR1; Site destroyed during cloning: No; Cloning site 3': attR2; Site destroyed during cloning: No; 5' Sequencing primer: n/a (List of Sequencing Primers); 3' Sequencing primer: n/a; Bacteria resistance: Ampicillin; High or low copy: High Copy; Gro... |
 | pLVCT-tTR-KRAB - Gene/insert name: CAG promoters, GFP, tTR-KRAB, Tet-on; Vector backbone: None (Search Vector Database); 5' Sequencing primer: See map (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: No; Bacterial strain for growth and growth condition: Use Stbl3 or HB101 to reduce chance of recombination. Grow at... |
| Plasmids 10 - 14 | Descriptions |
 | pMDLg/pRRE - Gene/insert name: HIV-1 GAG/POL; Species of gene(s): Other; Vector backbone: pMD (Search Vector Database); 5' Sequencing primer: CMV Fwd (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Plasmid Provided In: DH5a; Principal Investigator: Didier Trono; Comments: This 3rd generation LV packaging plasm... |
 | pRSV-Rev - Gene/insert name: Rev; Gene/insert aliases: rev; Species of gene(s): Other; Vector backbone: pRSV-Rev (Search Vector Database); 5' Sequencing primer: pREP fwd (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Plasmid Provided In: DH5a; Principal Investigator: Didier Trono; Comments: Rev cDNA expressin... |
 | pSin-EF2-Sox2-Pur - Gene/insert name: Homo sapiens SRY (sex determining region Y)-box 2 (SOX2); Alternative names: Sox2; GenBank/Entrez ID of insert: NM_003106; Gene/insert aliases: SOX2, ANOP3, MCOPS3, MGC2413; Species of gene(s): H. sapiens (human); Vector backbone: pSin4-EF2-IRES-Pur (Search Vector Database); Cloning site 5': EcoRI; Site destroyed during cloning: No; Cloning site ... |
 | psPAX2 - Gene/insert name: None; Vector backbone: psPAX2 (Search Vector Database); 5' Sequencing primer: See map (List of Sequencing Primers); Bacteria resistance: Ampicillin; High or low copy: High Copy; Grow in standard E. coli @ 37C: Yes; Plasmid Provided In: DH5a; Principal Investigator: Didier Trono; Comments: 2nd generation packaging vector. Known to work with many of the Aebischer and Tro... |
 | pTiger -
Backbone manufacturer: Nolan Lab ;
5' Sequencing primer: n/a ;
3' Sequencing primer: M13 reverse primer ;
Bacteria resistance: Ampicillin ;
High or low copy: Don't Know ;
Grow in standard E. coli @ 37C: Yes ;
Plasmid Provided In: DH5a;
Principal Investigator: Garry Nolan ;
Comments: This is a transfer vector (highest titer) from Nolan lab's FELIX vector system. T... |
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