qq(ID LORISTE6 preliminary; circular DNA; SYN; 5646 BP. XX AC ATCC37532; XX DT 01-JUL-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli cosmid vector LoristE6 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC LoristE6 from LoristB & oligo, trpA terminator RC Lorist2 from LoristE6 & oligo, rrnC terminator RA Gibson T.J., Coulson A.R., Sulston J.E., Little P.F.; RT "Lorist2, a cosmid with transcriptional terminators insulating RT vector genes from interference by promoters within the insert: RT effect on DNA yield and cloned insert frequency"; RL Gene 53:275-281(1987). XX RN [2] RC Lorist3 from Lorist2 & oligo, tet promoter RC Lorist4 from Lorist3 & linker RC Lorist6 from Lorist4 & oligo, trpA terminator RA Gibson T.J., Rosenthal A., Waterson R.H.; RT "Lorist6, a cosmid vector with BamHI, NotI, ScaI and HindIII cloning RT sites and altered neomycin phosphotransferase gene expression"; RL Gene 53:283-286(1987). XX CC Created by Moore, July 1995, under contract with NCBI. CC Accepts blunt-ended DNA. CC Cosmid vector for genomic library construction. Insert-end-specific CC probes may be generated from T7 and SP6 promoters. CC Restriction digests of the clone give the following sizes (kb): CC BamHI--5.7; HindIII--5.7. (ATCC staff) CC Medium is 1236 LB plus kanamycin. CC NM (LoristE6) CC CM (yes) CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (cosmid) CC SP (ATCC) CC HO (E.coli 1046)(E.coli) CC CP () CC FN (cloning) CC SE () CC PA (LoristB) CC BR () CC OF () CC OR () XX FH Key Location/Qualifiers FH FT misc_feature 0..0 FT /note="1. LoristB EcoRI 5614bp 531..531, may be 2393 FT 2. oligo EcoRI-trpA-EcoRI 32bp FT \ aattagcccgcctaatgagcgggctttttttg, trpA terminator FT -> LoristE6 5646bp" FT - 1..530 FT /note="LoristB 1..530 530bp FT EcoRI = G^AATTC" FT - 531..562 FT /note="aattagcccgcctaatgagcgggctttttttg 32bp FT EcoRI = G^AATTC" FT - 563..5646 FT /note="LoristB 531..5614 5084bp" FT promoter 0..0 FT /note="PRO bacteriophage T7" FT misc_binding 0..0 FT /note="MCS unique HindIII-NotI-ScaI-BamHI" FT promoter 0..0 FT /note="PRO bacteriophage Sp6" FT terminator 0..0 FT /note="TER E. coli rrnC" FT rep_origin 0..0 FT /note="ORI bacteriophage lambda" FT CDS 0..0 FT /note="GEN lambda P gene" FT CDS 0..0 FT /note="GEN lambda O gene" FT CDS 0..0 FT /note="GEN lambda cII gene" FT CDS 0..0 FT /note="GEN lambda cro gene" FT promoter 0..0 FT /note="PRO lambda pR" FT promoter 0..0 FT /note="PRO E. coli tet gene" FT CDS 0..0 FT /note="ANT E. coli neomycin phosphotransferase gene FT (NPT); neomycin resistance gene (neo)" FT terminator 0..0 FT /note="TER E. coli trpA" FT misc_binding 0..0 FT /note="SIT lambda cos" FT terminator 0..0 FT /note="TER E. coli trpA" XX SQ Sequence 5646 BP; 1285 A; 1467 C; 1448 G; 1446 T; 0 other; atcgatccgg gcaacgttgt tgccattgct gcaggggggg gggggggggg gggggggggt tgacttccat tgttcattcc acggacaaaa acagagaaag gaaacgacag aggccaaaaa gcctcgcttt cagcacctgt cgtttccttt cttttcagag ggtattttaa ataaaaacat taagttatga cgaagaagaa cggaaacgcc ttaaaccgga aaattttcat aaatagcgaa aacccgcgag gtcgccgccc gtaacctgtc ggatcaccgg aaaggacccg taaagtgata atgattatca tctacatatc acaacgtgcg tggaggccat caaaccacgt caaataatca attatgacgc aggtatcgta ttaattgatc tgcatcaact taacgtaaaa acaacttcag acaatacaaa tcagcgacac tgaatacggg gcaacctcat gtcaaccccc cccccccccc cccccccccc ctgcaggcgg agaactggta ggtatggaag atctctagag aattagcccg cctaatgagc gggctttttt tgaattcgag ctcgcccggg gatcgatcct ctagagtcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggcc cagctggctt atcgaaatta atacgactca ctatagggag accggaagct taggatccta tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatccccgg gcgagctgcg gcctgattta tgctggttac tgttgcgcct gttagcgcgg caacgtccgg cgcacagaag ctattatgcg tccccaggta atgaataatt gcctctttgc ccgtcataca cttgctcctt tcagtccgaa cttagctttg atttctgcga tcttcgccag agcctgtgca cgatttagag gtctaccgcc catgacagga agttgtttta ctggttcagg gatcgcctca ccacggttaa ttctcgcagt catatggaca agctcatctg cggccttacg gcgtaattcc gcatcagtaa gcgcattggc ccgcatgttc tgatacaggt tggtaaccag ccagtagtgc gcgtttgatt tccacggata agactccgca tccggataca ggcctcgctt ccggcaatac tcgtaaacca tatcaaccag ctcgctgacg tttggcagtc cggcggtaac ggatgcttct tcccggcacc atgcaacaaa ctgcccgggt gatggcagaa atggtcgatt ctgccgacgg gctacgcgca ttcctgcgtt aacctgttcc atcgtggtga tcccgttttc ccgaaaagcc agaacccact ggcgacggat ttcgttcact tcgttctggt cacggttagc caggctcgcc gggaaagttg ccagtaactg gctgaacaca ccgttgatga tctgcgctac ctgctgtacc tgcggctttt cgtcgtactg ttccggcatg ttgttggcga tccgacgcat ctgctcacgg tcaaagttaa ccatctgtgc ggcgatgttt ttcatagatc caccccgtaa atccagtctg tgtttgtcag gtcgagtttt ggtttgctgg ctgtcacgcc tgcctgttgc ttgttacggt tgatttcgag ttgggtccac ttatcgcgga gtttggccgg gctcagcacg ttaccggacc agaagttgtc ctggcatgcc cagcggaaca gcacacacat gtcgcggtgg ttacgtccgt cacgttcacg catcaggcgg atatcgttag cccacccagc aaaattcggt tttctggctg atggtgcgat agtcttcacc atgtcaaaca tccactctgc ggcggtcagg tcttctgctg tcccccactt gctgccgctc tgaattgcag catccggttt caccacagaa aggtcgtttt ctggctggtc agaggattcg ccagaattct ctgacgaata atcttttctt ttttcttttg taatagtgtc ttttgtgtcc ccctgttttg agggatagca atcccccaat ttgagggatg ttttatccct cgttttaggg gattttccct cgttttgagg gatgcaccat tctgagatgt ttttatttgg tccaaacatg ccgccttgct gcttgataat attcattctg acgagttcta acttggcttc attgcaccgt ttgacaggta actttgtaat ctcgctaagt tgagaatcgg tgattctgtc cattggttta ttccacccat aggttttacg cagaatggca agcagcactt taaactgtcg cttggtcaga tctgcgcccg aataagcctc aagcagcata tttgatagtc tggcgtaacc atcatcgaga tctgccacat tacgctcctg tccggcaaag ttacctctgc cgaagttgag tatttttgct gtatttgtca taatgactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc gcagcaactt gtcgcgccaa tcgagccatg tcgtcgtcaa cgacccccca ttcaagaaca gcaagcagca ttgagaactt tggaatccag tccctcttcc acctgctgat ctgcgactta tcaacgccca cagcttccgc tgtcttctca gttccaagca ttgcgatttt gttaagcaac gcactctcga ttcgtagagc ctcgttgcgt ttgtttgcac gaaccatatg taagtatttc cttagataac aattgattga atgtatgcaa ataaatgcat acaccatagg tgtggtttaa tttgatgccc tttttcaggg ctggaatgtg taagagcggg gttatttatg ctgttgtttt tttgttactc gggaagggct ttacctcttc cgcataaacg cttccatcag cgtttatagt taaaaaaatc tttcggcctg catgaatggc cttgttgatc gcgctttgat atacgccgag atctttagct gtcttggttt gcccaaagcg cattgcataa tctttcaggg ttatgcgttg ttccatacaa cctccttagt acatgcaacc attatcaccg ccagaggtaa aatagtcaac acgcacggtg ttagatattt atcccttgcg gtgatagatt taacgtatga gcacaaaaaa gaaaccatta acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttacaaaa attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga ccgcaccaac aggctccaag ccaagctagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcgacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccaccccg ggctcgatcc cctcgcgagt tggttcagct gctgcctgag gctggacgac ctcgcggagt tctaccggca gtgcaaatcc gtcggcatcc aggaaaccag cagcggctat ccgcgcatcc atgcccccga actgcaggag tggggaggca cgatggccgc tttggtcgga tcaattcgcg cgaccg //  LoristE6 "Times New RomanqC\cosN"Times New RomanqC. trpA_term"Times New RomanqCɆ:P pGEX_3_pri"Times New RomanqT7_pro"Times New RomanqCSp6_pri"Times New RomanqC"ORF 3"Times New RomanqCɆ_ ORF 2"Times New Romanq4 NEOKAN_pro"Times New RomanqC#ORF 2"Times New RomanqCJNeomycin(w/ gaps)"Times New Roman( qSacI"Times New Roman<@LOr4cjr qHindIII(926), BamHI(933)"Times New Romanw qStuI"Times New RomanB fjngu qEcoRV"Times New Roman ER^^  qBclI(4462), EagI(4527)"Times New Roman  qMscI(4703), FspI(4723)"Times New Romans  qNcoI"Times New Roman L?2|m2| qBstBI(5302), NruI(5475)"Times New RomanctaXI:X qtest"Times New RomansR