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    Plasmid map of Lox-Stop-Lox

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    Lox-Stop-Lox TOPO
    Gene/insert name: loxP-STOP-loxP
    Insert size (bp): 5700
    Species of gene(s): SV40
    Vector backbone: pCR-Blunt II-TOPO (Search Vector Database)
    Backbone manufacturer: Invitrogen
    Type of vector: Mammalian expression,Cre/Lox
    Backbone size (bp): 9267
    5' Sequencing primer: M13R (List of Sequencing Primers)
    Bacteria resistance: Kanamycin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Bacterial strain for growth and growth condition: However, plasmid yield and stability is improved when grown at 30 degrees.
    Selectable markers: Puromycin
    Plasmid Provided In: DH5a
    Principal Investigator: Tyler Jacks
    Comments: Plasmid containing LoxP-STOP-LoxP cassette for use in expressing genes only after cre-mediated recombination.
    Article: Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Jackson EL et al. (Genes Dev. 2001 Dec 15. 15(24):3243-8. Pubmed)

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