Sequence of Lox-Stop-Lox

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Sequence of Lox-Stop-Lox

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Sequence of Lox-Stop-Lox TOPO : 9267 BP; 2439 A; 2202 C; 2237 G; 2389 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60

1 tcgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg ctaagtaatt acgtcgaccg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120

61 tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt gcgttaatta cactcaatcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180

121 agtgagtaat ccgtggggtc cgaaatgtga aatacgaagg ccgagcatac aacacacctt 180

.......... .......... .......... lac .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 240

181 aacactcgcc tattgttaaa gtgtgtcctt tgtcgatact ggtactaatg cggttcgata 240

.......... M13_pUC_r .......... M13_r .......... ..........

.......... .......... .......... .......... .......... ..........

241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccaat ctcgagcctt 300

241 aatccactgt gatatcttat gagttcgata cgtagttcga accatggtta gagctcggaa 300

Sp6_pri .......... .......... .......... .......... ..........

.......... .......... SacI(329) .......... .......... ..........

301 aattaaccac gcgtggcgcg ccttgagctc ggatccgggc cccccctcga tcgaggtcga 360

301 ttaattggtg cgcaccgcgc ggaactcgag cctaggcccg ggggggagct agctccagct 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cataacttcg tataatgtat gctatacgaa gttattaggt ccctcgacct gcagacttac 420

361 gtattgaagc atattacata cgatatgctt caataatcca gggagctgga cgtctgaatg 420

.......... loxP .......... .......... .......... ..........

.......... NheI(434) EcoRI(445) .......... .......... ..........

421 tcaagccatg gtggctagct tgcggaattc cagacatgat aagatacatt gatgagtttg 480

421 agttcggtac caccgatcga acgccttaag gtctgtacta ttctatgtaa ctactcaaac 480

.......... .......... .......... ......... SV40_PA_term EBV_r

.......... .......... .......... .......... .......... ..........

481 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta 540

481 ctgtttggtg ttgatcttac gtcacttttt ttacgaaata aacactttaa acactacgat 540

EBV_r ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc 600

541 aacgaaataa acattggtaa tattcgacgt tatttgttca attgttgttg ttaacgtaag 600

......... SV40_PA_term ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct 660

601 taaaatacaa agtccaagtc cccctccaca ccctccaaaa aatttcgttc attttggaga 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acaaatgtgg tatggctgat tatgatctct agtcaaggca ctatacatca aatattcctt 720

661 tgtttacacc ataccgacta atactagaga tcagttccgt gatatgtagt ttataaggaa 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 attaacccct ttacaaatta aaaagctaaa ggtacacaat ttttgagcat agttattaat 780

721 taattgggga aatgtttaat ttttcgattt ccatgtgtta aaaactcgta tcaataatta 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agcagacact ctatgcctgt gtggagtaag aaaaaacagt atgttatgat tataactgtt 840

781 tcgtctgtga gatacggaca cacctcattc ttttttgtca tacaatacta atattgacaa 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atgcctactt ataaaggtta cagaatattt ttccataatt ttcttgtata gcagtgcagc 900

841 tacggatgaa tatttccaat gtcttataaa aaggtattaa aagaacatat cgtcacgtcg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tttttccttt gtggtgtaaa tagcaaagca agcaagagtt ctattactaa acacagcatg 960

901 aaaaaggaaa caccacattt atcgtttcgt tcgttctcaa gataatgatt tgtgtcgtac 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 actcaaaaaa cttagcaatt ctgaaggaaa gtccttgggg tcttctacct ttctcttctt 1020

961 tgagtttttt gaatcgttaa gacttccttt caggaacccc agaagatgga aagagaagaa 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttttggagga gtagaatgtt gagagtcagc agtagcctca tcatcactag atggcatttc 1080

1021 aaaacctcct catcttacaa ctctcagtcg tcatcggagt agtagtgatc taccgtaaag 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttctgagcaa aacaggtttt cctcattaaa ggcattccac cactgctccc attcatcagt 1140

1081 aagactcgtt ttgtccaaaa ggagtaattt ccgtaaggtg gtgacgaggg taagtagtca 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tccataggtt ggaatctaaa atacacaaac aattagaatc agtagtttaa cacattatac 1200

1141 aggtatccaa ccttagattt tatgtgtttg ttaatcttag tcatcaaatt gtgtaatatg 1200

.......... .......... ........ SV40_3_splice ........ ..........

.......... .......... .......... .......... .......... ..........

1201 acttaaaaat tttatattta ccttagagct ttaaatctct gtaggtagtt tgtccaatta 1260

1201 tgaattttta aaatataaat ggaatctcga aatttagaga catccatcaa acaggttaat 1260

.......... SV40_int .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgtcacacca cagaagtaag gttccttcac aaagatccct cgaccagaca tgataagata 1320

1261 acagtgtggt gtcttcattc caaggaagtg tttctaggga gctggtctgt actattctat 1320

.......... .......... .......... .......... ......... SV40_PA_term

.......... .......... .......... .......... .......... ..........

1321 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga 1380

1321 gtaactactc aaacctgttt ggtgttgatc ttacgtcact ttttttacga aataaacact 1380

........ EBV_rev_pri SV40_PA_term ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa 1440

1381 ttaaacacta cgataacgaa ataaacattg gtaatattcg acgttatttg ttcaattgtt 1440

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag 1500

1441 gttgttaacg taagtaaaat acaaagtcca agtccccctc cacaccctcc aaaaaatttc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 caagtaaaac ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac 1560

1501 gttcattttg gagatgttta caccataccg actaatacta gagatcagtt ccgtgatatg 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atcaaatatt ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga 1620

1561 tagtttataa ggaataattg gggaaatgtt taatttttcg atttccatgt gttaaaaact 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcatagttat taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta 1680

1621 cgtatcaata attatcgtct gtgagatacg gacacacctc attctttttt gtcatacaat 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgattataac tgttatgcct acttataaag gttacagaat atttttccat aattttcttg 1740

1681 actaatattg acaatacgga tgaatatttc caatgtctta taaaaaggta ttaaaagaac 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tatagcagtg cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta 1800

1741 atatcgtcac gtcgaaaaag gaaacaccac atttatcgtt tcgttcgttc tcaagataat 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctaaacacag catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct 1860

1801 gatttgtgtc gtactgagtt ttttgaatcg ttaagacttc ctttcaggaa ccccagaaga 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acctttctct tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca 1920

1861 tggaaagaga agaaaaaacc tcctcatctt acaactctca gtcgtcatcg gagtagtagt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctagatggca tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc 1980

1921 gatctaccgt aaagaagact cgttttgtcc aaaaggagta atttccgtaa ggtggtgacg 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt 2040

1981 agggtaagta gtcaaggtat ccaaccttag attttatgtg tttgttaatc ttagtcatca 2040

.......... .......... .......... ........ SV40_3_splice ........

.......... .......... .......... .......... .......... ..........

2041 ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt 2100

2041 aattgtgtaa tatgtgaatt tttaaaatat aaatggaatc tcgaaattta gagacatcca 2100

SV40_3_splice ...... SV40_int .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagat ccctcgacca 2160

2101 tcaaacaggt taatacagtg tggtgtcttc attccaagga agtgtttcta gggagctggt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa 2220

2161 ctgtactatt ctatgtaact actcaaacct gtttggtgtt gatcttacgt cacttttttt 2220

.......... ........ EBV_rev_pri SV40_PA_term ........ ..........

.......... .......... .......... .......... .......... ..........

2221 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat 2280

2221 acgaaataaa cactttaaac actacgataa cgaaataaac attggtaata ttcgacgtta 2280

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg 2340

2281 tttgttcaat tgttgttgtt aacgtaagta aaatacaaag tccaagtccc cctccacacc 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta tgatctctag 2400

2341 ctccaaaaaa tttcgttcat tttggagatg tttacaccat accgactaat actagagatc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tcaaggcact atacatcaaa tattccttat taaccccttt acaaattaaa aagctaaagg 2460

2401 agttccgtga tatgtagttt ataaggaata attggggaaa tgtttaattt ttcgatttcc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tacacaattt ttgagcatag ttattaatag cagacactct atgcctgtgt ggagtaagaa 2520

2461 atgtgttaaa aactcgtatc aataattatc gtctgtgaga tacggacaca cctcattctt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aaaacagtat gttatgatta taactgttat gcctacttat aaaggttaca gaatattttt 2580

2521 ttttgtcata caatactaat attgacaata cggatgaata tttccaatgt cttataaaaa 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccataatttt cttgtatagc agtgcagctt tttcctttgt ggtgtaaata gcaaagcaag 2640

2581 ggtattaaaa gaacatatcg tcacgtcgaa aaaggaaaca ccacatttat cgtttcgttc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 caagagttct attactaaac acagcatgac tcaaaaaact tagcaattct gaaggaaagt 2700

2641 gttctcaaga taatgatttg tgtcgtactg agttttttga atcgttaaga cttcctttca 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccttggggtc ttctaccttt ctcttctttt ttggaggagt agaatgttga gagtcagcag 2760

2701 ggaaccccag aagatggaaa gagaagaaaa aacctcctca tcttacaact ctcagtcgtc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tagcctcatc atcactagat ggcatttctt ctgagcaaaa caggttttcc tcattaaagg 2820

2761 atcggagtag tagtgatcta ccgtaaagaa gactcgtttt gtccaaaagg agtaatttcc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cattccacca ctgctcccat tcatcagttc cataggttgg aatctaaaat acacaaacaa 2880

2821 gtaaggtggt gacgagggta agtagtcaag gtatccaacc ttagatttta tgtgtttgtt 2880

.......... .......... .......... .......... ........ SV40_3_splice

.......... .......... .......... .......... .......... ..........

2881 ttagaatcag tagtttaaca cattatacac ttaaaaattt tatatttacc ttagagcttt 2940

2881 aatcttagtc atcaaattgt gtaatatgtg aatttttaaa atataaatgg aatctcgaaa 2940

........ SV40_3_splice ........ .......... SV40_int ..........

.......... .......... .......... .......... .......... ..........

2941 aaatctctgt aggtagtttg tccaattatg tcacaccaca gaagtaaggt tccttcacaa 3000

2941 tttagagaca tccatcaaac aggttaatac agtgtggtgt cttcattcca aggaagtgtt 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 agatccctcg accagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa 3060

3001 tctagggagc tggtctgtac tattctatgt aactactcaa acctgtttgg tgttgatctt 3060

.......... .......... ........ SV40_PA_term EBV_rev_pri ........

.......... .......... .......... .......... .......... ..........

3061 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca 3120

3061 acgtcacttt ttttacgaaa taaacacttt aaacactacg ataacgaaat aaacattggt 3120

.......... ......... SV40_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc 3180

3121 aatattcgac gttatttgtt caattgttgt tgttaacgta agtaaaatac aaagtccaag 3180

SV40_PA_term ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg 3240

3181 tccccctcca caccctccaa aaaatttcgt tcattttgga gatgtttaca ccataccgac 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 attatgatct ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat 3300

3241 taatactaga gatcagttcc gtgatatgta gtttataagg aataattggg gaaatgttta 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 taaaaagcta aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct 3360

3301 atttttcgat ttccatgtgt taaaaactcg tatcaataat tatcgtctgt gagatacgga 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gtgtggagta agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt 3420

3361 cacacctcat tcttttttgt catacaatac taatattgac aatacggatg aatatttcca 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tacagaatat ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta 3480

3421 atgtcttata aaaaggtatt aaaagaacat atcgtcacgt cgaaaaagga aacaccacat 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aatagcaaag caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa 3540

3481 ttatcgtttc gttcgttctc aagataatga tttgtgtcgt actgagtttt ttgaatcgtt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttctgaagga aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg 3600

3541 aagacttcct ttcaggaacc ccagaagatg gaaagagaag aaaaaacctc ctcatcttac 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttgagagtca gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt 3660

3601 aactctcagt cgtcatcgga gtagtagtga tctaccgtaa agaagactcg ttttgtccaa 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttcctcatta aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta 3720

3661 aaggagtaat ttccgtaagg tggtgacgag ggtaagtagt caaggtatcc aaccttagat 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aaatacacaa acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt 3780

3721 tttatgtgtt tgttaatctt agtcatcaaa ttgtgtaata tgtgaatttt taaaatataa 3780

.......... ........ SV40_3_splice ........ .......... SV40_int

.......... .......... .......... .......... .......... ..........

3781 taccttagag ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta 3840

3781 atggaatctc gaaatttaga gacatccatc aaacaggtta atacagtgtg gtgtcttcat 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aggttccttc acaaagatcc ctcgatcgag aaaaaaaata taaaagagat ggaggaacgg 3900

3841 tccaaggaag tgtttctagg gagctagctc ttttttttat attttctcta cctccttgcc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gaaaaagtta gttgtggtga taggtggcaa gtggtattcc gtaagaacaa caagaaaagc 3960

3901 ctttttcaat caacaccact atccaccgtt caccataagg cattcttgtt gttcttttcg 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atttcatatt atggctgaac tgagcgaaca agtgcaaaat ttaagcatca acgacaacaa 4020

3961 taaagtataa taccgacttg actcgcttgt tcacgtttta aattcgtagt tgctgttgtt 4020

.......... .......... .......... .......... .......... ..........

.......... ......... AflII(4045) ......... .......... ..........

4021 cgagaatggt tatgttcctc ctcacttaag aggaaaacca agaagtgcca gaaataacat 4080

4021 gctcttacca atacaaggag gagtgaattc tccttttggt tcttcacggt ctttattgta 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gagcaactac aataacaaca acggcggcta caacggtggc cgtggcggtg gcagcttctt 4140

4081 ctcgttgatg ttattgttgt tgccgccgat gttgccaccg gcaccgccac cgtcgaagaa 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tagcaacaac cgtcgtggtg gttacggcaa cggtggtttc ttcggtggaa acaacggtgg 4200

4141 atcgttgttg gcagcaccac caatgccgtt gccaccaaag aagccacctt tgttgccacc 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagcagatct aacggccgtt ctggtggtag atggatcgat ggcaaacatg tcccagctcc 4260

4201 gtcgtctaga ttgccggcaa gaccaccatc tacctagcta ccgtttgtac agggtcgagg 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aagaaacgaa aaggccgaga tcgccatatt tggtgtcccc gaggatccct gtggaaaaaa 4320

4261 ttctttgctt ttccggctct agcggtataa accacagggg ctcctaggga cacctttttt 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aagggacagg ataagtatga catcatcaag gaaaccctgg actactgcgc ccttcgacct 4380

4321 ttccctgtcc tattcatact gtagtagttc ctttgggacc tgatgacgcg ggaagctgga 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag 4440

4381 gctttaagat ggcccatccc ctccgcgaaa agggttccgt cagacctcgt acgcgaaatc 4440

.......... .......... .......... .......... ......... MSCV_rev_pri

.......... .......... .......... .......... .......... ..........

4441 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat 4500

4441 gtcggggcga cccgtgaacc gcgatgtgtt caccggagac cggagcgtgt gtaaggtgta 4500

MSCV_rev_pri ........ .......... .......... .......... ..........

AgeI(4503) .......... .......... .......... .......... ..........

4501 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc 4560

4501 ggtggccatc cgcggttggc cgaggcaaga aaccaccggg gaagcgcggt ggaagatgag 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa 4620

4561 gaggggatca gtccttcaag ggggggcggg gcgtcgagcg cagcacgtcc tgcactgttt 4620

.......... .......... .......... .......... .......... ..........

.......... SpeI(4638) .......... .......... .......... ..........

4621 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc 4680

4621 accttcatcg tgcagagtga tcagagcacg tctacctgtc gtggcgactc gttaccttcg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga 4740

4681 cccatccgga aaccccgtcg ccggttatcg tcgaaacgag gaagcgaaag acccgagtct 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg 4800

4741 ccgacccttc cccacccagg cccccgcccg agtccccgcc cgagtccccg ccccgcccgc 4800

.......... .......... .......... .......... .......... ORF 1

.......... .......... .......... .......... .......... ..........

4801 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc 4860

4801 gggcttccag gaggcctccg ggccgtaaga cgtgcgaagt tttcgcgtgc agacggcgcg 4860

.......... .......... ORF 1 mPGK_F_pri .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tgttctcctc ttcctcatct ccgggccttt cgacctgcat ccatctagat ctcgatcgag 4920

4861 acaagaggag aaggagtaga ggcccggaaa gctggacgta ggtagatcta gagctagctc 4920

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cagctgaagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 4980

4921 gtcgacttcg aatggtactg gctcatgttc gggtgccacg cggagcggtg ggcgctgctg 4980

.......... .......... ORF 1 ORF 1 puro .......... ..........

.......... .......... .......... .......... .......... ..........

4981 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5040

4981 caggggtccc ggcatgcgtg ggagcggcgg cgcaagcggc tgatggggcg gtgcgcggtg 5040

.......... ....... ORF 1 ORF 1 puro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5100

5041 tggcagctag gcctggcggt gtagctcgcc cagtggctcg acgttcttga gaaggagtgc 5100

.......... ....... ORF 1 ORF 1 puro ....... .......... ..........

.......... .......... .......... ......... SacII(5150) .........

5101 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 5160

5101 gcgcagcccg agctgtagcc gttccacacc cagcgcctgc tgccgcggcg ccaccgccag 5160

.......... ....... ORF 1 ORF 1 puro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 5220

5161 acctggtgcg gcctctcgca gcttcgcccc cgccacaagc ggctctagcc gggcgcgtac 5220

.......... ....... ORF 1 ORF 1 puro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 5280

5221 cggctcaact cgccaagggc cgaccggcgc gtcgttgtct accttccgga ggaccgcggc 5280

.......... ....... ORF 1 ORF 1 puro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 5340

5281 gtggccgggt tcctcgggcg caccaaggac cggtggcagc cgcagagcgg gctggtggtc 5340

.......... ....... ORF 1 ORF 1 puro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 5400

5341 ccgttcccag acccgtcgcg gcagcacgag gggcctcacc tccgccggct cgcgcggccc 5400

.......... ....... ORF 1 ORF 1 puro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 5460

5401 cacgggcgga aggacctctg gaggcgcggg gcgttggagg ggaagatgct cgccgagccg 5460

.......... ....... ORF 1 ORF 1 puro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 5520

5461 aagtggcagt ggcggctgca gctccacggg cttcctggcg cgtggaccac gtactgggcg 5520

.......... ....... ORF 1 ORF 1 puro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aagcccggtg cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga 5580

5521 ttcgggccac ggactgcggg cggggtgctg ggcgtcgcgg gctggctttc ctcgcgtgct 5580

ORF 1 puro .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ccccatgcat cgatgatatc agatccccgg gatgcagaaa ttgatgatct attaaacaat 5640

5581 ggggtacgta gctactatag tctaggggcc ctacgtcttt aactactaga taatttgtta 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca 5700

5641 tttctacagg tgattttacc ttcaaaaagg acagtatgaa acaattcttc ccactcttgt 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta 5760

5701 ctcatggatg taaaacttac cttcctaacc tcgatgcccc cacccccacc ccaccctaat 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag 5820

5761 ctatttacgg acgagaaatg acttccgaga aatgataacg aaatactatt acaaagtatc 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc 5880

5821 aacctatagt attaaatttg ttcgttttgg tttaattccc ggtcgagtaa ggagggtgag 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 atgatctata gatctataga tctctcgtgg gatcattgtt tttctcttga ttcccacttt 5940

5881 tactagatat ctagatatct agagagcacc ctagtaacaa aaagagaact aagggtgaaa 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gtggttctaa gtactgtggt ttccaaatgt gtcagtttca tagcctgaag aacgagatca 6000

5941 caccaagatt catgacacca aaggtttaca cagtcaaagt atcggacttc ttgctctagt 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gcagcctctg ttccacatac acttcattct cagtattgtt ttgccaagtt ctaattccat 6060

6001 cgtcggagac aaggtgtatg tgaagtaaga gtcataacaa aacggttcaa gattaaggta 6060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NotI(6118)

6061 cagaagctgg tcgagataac ttcgtataat gtatgctata cgaagttatg tcgacagcgg 6120

6061 gtcttcgacc agctctattg aagcatatta catacgatat gcttcaatac agctgtcgcc 6120

.......... .......... .......... loxP .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ccgctcgagc atgcatctag agggcccaat tcgccctata gtgagtcgta ttacaattca 6180

6121 ggcgagctcg tacgtagatc tcccgggtta agcgggatat cactcagcat aatgttaagt 6180

.......... .......... .......... .......... T7_pro ..........

.......... .......... .......... .......... .......... ..........

6181 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc 6240

6181 gaccggcagc aaaatgttgc agcactgacc cttttgggac cgcaatgggt tgaattagcg 6240

M13_f20 M13_pUC_f lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc 6300

6241 gaacgtcgtg tagggggaaa gcggtcgacc gcattatcgc ttctccgggc gtggctagcg 6300

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 ccttcccaac agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga 6360

6301 ggaagggttg tcaacgcgtc ggatatgcat gccgtcaaat tccaaatgtg gatattttct 6360

lacZ_a .......... .......... .......... .......... ccdB

.......... .......... .......... .......... .......... ..........

6361 gagagccgtt atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga 6420

6361 ctctcggcaa tagcagacaa acacctacat gtctcactat aataactgtg cggccccgct 6420

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 cggatggtga tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt 6480

6421 gcctaccact agggggaccg gtcacgtgca gacgacagtc tatttcagag ggcacttgaa 6480

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 tacccggtgg tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt 6540

6481 atgggccacc acgtatagcc cctactttcg accgcgtact actggtggct ataccggtca 6540

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gtgccggtct ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc 6600

6541 cacggccaga ggcaatagcc ccttcttcac cgactagagt cggtggcgct tttactgtag 6600

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 aaaaacgcca ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa 6660

6601 tttttgcggt aattggacta caagacccct tatatttaca gtccgtactc taatagtttt 6660

.......... ccdB .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 aggatcttca cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc 6720

6661 tcctagaagt ggatctagga aaagtgcatc tttcggtcag gcgtctttgc cacgactggg 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 cggatgaatg tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag 6780

6721 gcctacttac agtcgatgac ccgatagacc tgttcccttt tgcgttcgcg tttctctttc 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 caggtagctt gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca 6840

6781 gtccatcgaa cgtcacccga atgtaccgct atcgatctga cccgccaaaa tacctgtcgt 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 agcgaaccgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta 6900

6841 tcgcttggcc ttaacggtcg accccgcggg agaccattcc aacccttcgg gacgtttcat 6900

.......... .......... .......... NEOKAN_pro .......... ..........

.......... .......... .......... .......... .......... BclI(6954)

6901 aactggatgg ctttctcgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa 6960

6901 ttgacctacc gaaagagcgg cggttcctag actaccgcgt cccctagttc gagactagtt 6960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg 7020

6961 ctctgtccta ctcctagcaa agcgtactaa cttgttctac ctaacgtgcg tccaagaggc 7020

.......... .......... .......... ....... ORF 1 NeoR/KanR .......

.......... .......... .......... .......... .......... ..........

7021 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct 7080

7021 cggcgaaccc acctctccga taagccgata ctgacccgtg ttgtctgtta gccgacgaga 7080

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac 7140

7081 ctacggcggc acaaggccga cagtcgcgtc cccgcgggcc aagaaaaaca gttctggctg 7140

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg 7200

7141 gacaggccac gggacttact tgacgttctg ctccgtcgcg ccgatagcac cgaccggtgc 7200

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg 7260

7201 tgcccgcaag gaacgcgtcg acacgagctg caacagtgac ttcgcccttc cctgaccgac 7260

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa 7320

7261 gataacccgc ttcacggccc cgtcctagag gacagtagag tggaacgagg acggctcttt 7320

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca 7380

7321 cataggtagt accgactacg ttacgccgcc gacgtatgcg aactaggccg atggacgggt 7380

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt 7440

7381 aagctggtgg ttcgctttgt agcgtagctc gctcgtgcat gagcctacct tcggccagaa 7440

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc 7500

7441 cagctagtcc tactagacct gcttctcgta gtccccgagc gcggtcggct tgacaagcgg 7500

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc 7560

7501 tccgagttcc gctcgtacgg gctgccgctc ctagagcagc actgggtacc gctacggacg 7560

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg 7620

7561 aacggcttat agtaccacct tttaccggcg aaaagaccta agtagctgac accggccgac 7620

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt 7680

7621 ccacaccgcc tggcgatagt cctgtatcgc aaccgatggg cactataacg acttctcgaa 7680

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag 7740

7681 ccgccgctta cccgactggc gaaggagcac gaaatgccat agcggcgagg gctaagcgtc 7740

.......... ....... ORF 1 NeoR/KanR ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc 7800

7741 gcgtagcgga agatagcgga agaactgctc aagaagactt aataattgcg aatgttaaag 7800

....... ORF 1 NeoR/KanR ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat acaggtggca 7860

7801 gactacgcca taaaagagga atgcgtagac acgccataaa gtgtggcgta tgtccaccgt 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 7920

7861 gaaaagcccc tttacacgcg ccttggggat aaacaaataa aaagatttat gtaagtttat 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatagcac gtgaggaggg 7980

7921 acataggcga gtactctgtt attgggacta tttacgaagt tattatcgtg cactcctccc 7980

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ccaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag 8040

7981 ggtggtaccg gttcaactgg tcacggcaag gccacgagtg gcgcgcgctg cagcggcctc 8040

.......... .......... ......... bleo sh_ble ......... ..........

.......... .......... .......... .......... .......... ..........

8041 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg 8100

8041 gccagctcaa gacctggctg gccgagccca agagggccct gaagcacctc ctgctgaagc 8100

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8101 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc 8160

8101 ggccacacca ggccctgctg cactgggaca agtagtcgcg ccaggtcctg gtccaccacg 8160

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8161 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt 8220

8161 gcctgttgtg ggaccggacc cacacccacg cgccggacct gctcgacatg cggctcacca 8220

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8221 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg 8280

8221 gcctccagca caggtgcttg aaggccctgc ggaggcccgg ccggtactgg ctctagccgc 8280

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8281 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg 8340

8281 tcgtcggcac ccccgccctc aagcgggacg cgctgggccg gccgttgacg cacgtgaagc 8340

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

8341 tggccgagga gcaggactga cacgtgctaa aacttcattt ttaatttaaa aggatctagg 8400

8341 accggctcct cgtcctgact gtgcacgatt ttgaagtaaa aattaaattt tcctagatcc 8400

bleo sh_ble ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8401 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 8460

8401 acttctagga aaaactatta gagtactggt tttagggaat tgcactcaaa agcaaggtga 8460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

8461 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 8520

8461 ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa aaagacgcgc 8520

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

8521 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 8580

8521 attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca aacggcctag 8580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8581 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 8640

8581 ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc tatggtttat 8640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8641 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 8700

8641 gacaggaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat cgtggcggat 8700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8701 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 8760

8701 gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta ttcagcacag 8760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8761 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 8820

8761 aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc ccgacttgcc 8820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8821 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 8880

8821 ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac tctatggatg 8880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8881 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 8940

8881 tcgcactcga tactctttcg cggtgcgaag ggcttccctc tttccgcctg tccataggcc 8940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

8941 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 9000

8941 attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct ttgcggacca 9000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9001 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 9060

9001 tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa aacactacga 9060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9061 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 9120

9061 gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat gccaaggacc 9120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

9121 gcttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 9180

9121 cgaaaacgac cggaaaacga gtgtacaaga aaggacgcaa taggggacta agacacctat 9180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9181 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 9240

9181 tggcataatg gcggaaactc actcgactat ggcgagcggc gtcggcttgc tggctcgcgt 9240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

9241 gcgagtcagt gagcgaggaa gcggaag... .......... .......... .......... 9267

9241 cgctcagtca ctcgctcctt cgccttc... .......... .......... .......... 9267

.......... .......... .......... .......... .......... ..........