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Luciferase Plasmids


 FHRE-Luc to pAG184_NEK6 | pAG193_CORO1C to PGC-1-luciferase | pIS0 to pRL-TK_CXCR4_2x | 

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Plasmids 0 - 4Descriptions
small image of FHRE-LucFHRE-Luc - Gene/insert name: FHRE; Alternative names: FKHRL1; FOXO3a; forkhead responsive element; Gene/insert aliases: FOXO3, FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, MGC12739, MGC31925, DKFZp781A0677; Species of gene(s): H. sapiens (human); Relevant mutations/deletions: 3 copies of FRE; Vector backbone: pGL3 basic (Search Vector Database); Backbone manufacturer: Promega; Cloning site 5'':...
small image of pAG142_TNS3pAG142 TNS3 3'UTR - Gene/insert name: N7 3'UTR; Alternative names: TNS3; Tensin 3; GenBank/Entrez ID of insert: NM_022748; Species of gene(s): H. sapiens (human); Vector backbone: pIS2 (Search Vector Database); Backbone manufacturer: pIS2 derived from pRL-SV40 (Promega); Cloning site 5': SacI; Site destroyed during cloning: No; Cloning site 3': SpeI; Site destroyed during cloning: No...
small image of pAG143_AK024929pAG143 AK024929 3'UTR - Gene/insert name: N8 3'UTR; GenBank/Entrez ID of insert: AK024929; Species of gene(s): H. sapiens (human); Vector backbone: pIS2 (Search Vector Database); Backbone manufacturer: pIS2 derived from pRL-SV40 (Promega); Cloning site 5': SacI; Site destroyed during cloning: No; Cloning site 3': SpeI; Site destroyed during cloning: No; 5' Sequencing primer: EBV re...
small image of pAG147_BC072452pAG147 BC072452 3'UTR - Gene/insert name: N12 3'UTR; GenBank/Entrez ID of insert: BC072452; Species of gene(s): H. sapiens (human); Vector backbone: pIS2 (Search Vector Database); Backbone manufacturer: pIS2 derived from pRL-SV40 (Promega); Cloning site 5': SacI; Site destroyed during cloning: No; Cloning site 3': NheI; Site destroyed during cloning: No; 5' Sequencing primer: EBV r...
small image of pAG184_NEK6pAG184 NEK6 3'UTR - Gene/insert name: N17 3'UTR; Alternative names: NEK6; GenBank/Entrez ID of insert: NM_014397; Gene/insert aliases: NEK6, SID6-1512; Species of gene(s): H. sapiens (human); Vector backbone: pIS2 (Search Vector Database); Backbone manufacturer: pIS2 derived from pRL-SV40 (Promega); Cloning site 5': SacI; Site destroyed during cloning: No; Cloning site 3': SpeI; Site d...

Plasmids 5 - 9Descriptions
small image of pAG193_CORO1CpAG193 CORO1C 3'UTR - Gene/insert name: CORO1C 3'UTR; Alternative names: CORO1C; GenBank/Entrez ID of insert: NM_014325; Gene/insert aliases: CORO1C, HCRNN4; Species of gene(s): H. sapiens (human); Vector backbone: pIS2 (Search Vector Database); Backbone manufacturer: pIS2 derived from pRL-SV40 (Promega); Cloning site 5': SacI; Site destroyed during cloning: No; Cloning site 3': SpeI...
small image of pBV-LucpBV-Luc - Gene/insert name: None; Fusion proteins or tags: Luciferase; Terminal: C terminal on backbone; Vector backbone: pBV-Luc (Search Vector Database); Backbone manufacturer: Vogelstein Lab; Cloning site 5': See map; Site destroyed during cloning: No; Cloning site 3': See map; Site destroyed during cloning: No; 5' Sequencing primer: RVprimer3 (List of Sequencing Primers); 3' Sequencin...
small image of PGC-1PGC-1 alpha promoter luciferase delta MEF - Gene/insert name: PGC-1 alpha promoter dMEF; Alternative names: PGC1 promoter; PGC-1a promoter; Gene/insert aliases: Ppargc1a, Pgc1, PGC-1, Pgco1, PGC-1v, Ppargc1, Pgc-1alpha, A830037N07Rik; Species of gene(s): M. musculus (mouse); Relevant mutations/deletions: Site-directed mutagenesis to remove the MEF2 binding site.; Fusion proteins or tags: luciferase...
small image of PGC-1-CrePGC-1 alpha promoter luciferase delta CRE - Gene/insert name: PGC-1 alpha promoter dCRE; Alternative names: PGC1 promoter; PGC-1a promoter; Gene/insert aliases: Ppargc1a, Pgc1, PGC-1, Pgco1, PGC-1v, Ppargc1, Pgc-1alpha, A830037N07Rik; Species of gene(s): M. musculus (mouse); Relevant mutations/deletions: Site-directed mutagenesis to remove the CREB binding site.; Fusion proteins or tags: lucife...
small image of PGC-1-luciferasePGC-1 alpha promoter 2kb luciferase - Gene/insert name: PGC-1 alpha promoter; Alternative names: PGC1 promoter; PGC-1a promoter; Gene/insert aliases: Ppargc1a, Pgc1, PGC-1, Pgco1, PGC-1v, Ppargc1, Pgc-1alpha, A830037N07Rik; Species of gene(s): M. musculus (mouse); Fusion proteins or tags: luciferase; Terminal: C terminal on backbone; Vector backbone: pGL3-basic (Search Vector Database...

Plasmids 10 - 14Descriptions
small image of pIS0Vector backbone: pIS0 - Backbone manufacturer: Bartel Lab ; 5' Sequencing primer: n/a ; 3' Sequencing primer: EBV rev primer (GTGGTTTGTCCAAACTCATC) ; Bacteria resistance: Ampicillin ; High or low copy: High Copy ; Grow in standard E. coli @ 37C: Yes ; Plasmid Provided In: DH5a ; Principal Investigator: David Bartel ; Comments: The firefly luciferase vector was modified fr...
small image of pIS1Vector backbone: pIS1 - Backbone manufacturer: Bartel Lab ; 5' Sequencing primer: n/a ; 3' Sequencing primer: EBV rev primer (GTGGTTTGTCCAAACTCATC) ; Bacteria resistance: Ampicillin ; High or low copy: High Copy ; Grow in standard E. coli @ 37C: Yes ; Plasmid Provided In: DH5a ; Principal Investigator: David Bartel ; Comments: pIS1 was derived from pRL-TK by adding more c...
small image of pIS2Vector backbone: pIS2 - Backbone manufacturer: David Bartel Lab ; 5' Sequencing primer: EBV rev primer (GTGGTTTGTCCAAACTCATC) (List of Sequencing Primers) ; Bacteria resistance: Ampicillin ; High or low copy: High Copy ; Grow in standard E. coli @ 37C: Yes ; Plasmid Provided In: DH5a ; Principal Investigator: David Bartel ; Comments: pIS2 was derived from pRL-SV40. More cloni...
small image of pRL-TK CXCR4 2xpRL-TK CXCR4 2x - Gene/insert name: 2 bulged bind sites for CXCR4 siRNA antisense ; Fusion proteins or tags: Rr-luc ; Terminal: N terminal on backbone ; Vector backbone: pRL-TK ; Backbone manufacturer: Promega ; Cloning site 5': XbaI ; Site destroyed during cloning: No ; Cloning site 3': ApaI ; Site destroyed during cloning: No ; 5' Sequencing primer: See map ; 3...
small image of pRL-TK_CXCR4_2xpRL-TK CXCR4 2x - Gene/insert name: 2 bulged bind sites for CXCR4 siRNA antisense; Fusion proteins or tags: Rr-luc; Terminal: N terminal on backbone; Vector backbone: pRL-TK (Search Vector Database); Backbone manufacturer: Promega; Cloning site 5': XbaI; Site destroyed during cloning: No; Cloning site 3': ApaI; Site destroyed during cloning: No; 5' Sequencing primer: See map (List...



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