Gene/insert name: Envelope
Alternative names: Env, FrMLVgp3
Insert size (bp): 2800
Gene/insert aliases: env
Species of gene(s): MuLV
Fusion proteins or tags: EYFP
Terminal: Unknown
Vector backbone: pcDNA3
Type of vector: Mammalian expression
Backbone size (bp): 8800
Cloning site 5': ?
Site destroyed during cloning: Don't Know
Cloning site 3': ?
Site destroyed during cloning: Don't Know
5' Sequencing primer: T7
3' Sequencing primer: SP6
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: Neomycin
Plasmid Provided In: DH5a
Comments: YFP was cloned into the NheI site of pFr-MuLV273/274 from Abraham Pinter. The entire Env gene was then transferred into pcDNA3.
Article: Visualization of retroviral replication in living cells reveals budding into multivesicular bodies. Sherer NM et al. (Traffic 2003 Nov;4(11):785-801. Pubmed)