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    Plasmid map of Friend MLV Env YFP

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    Friend MLV Env YFP
    Gene/insert name: Envelope
    Alternative names: Env, FrMLVgp3
    Insert size (bp): 2800
    Gene/insert aliases: env
    Species of gene(s): MuLV
    Fusion proteins or tags: EYFP
    Terminal: Unknown
    Vector backbone: pcDNA3
    Type of vector: Mammalian expression
    Backbone size (bp): 8800
    Cloning site 5': ?
    Site destroyed during cloning: Don't Know
    Cloning site 3': ?
    Site destroyed during cloning: Don't Know
    5' Sequencing primer: T7
    3' Sequencing primer: SP6
    Bacteria resistance: Ampicillin
    High or low copy: High Copy
    Grow in standard E. coli @ 37C: Yes
    Selectable markers: Neomycin
    Plasmid Provided In: DH5a
    Comments: YFP was cloned into the NheI site of pFr-MuLV273/274 from Abraham Pinter. The entire Env gene was then transferred into pcDNA3.
    Article: Visualization of retroviral replication in living cells reveals budding into multivesicular bodies. Sherer NM et al. (Traffic 2003 Nov;4(11):785-801. Pubmed)

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